In this study, we present a convenient approach utilizing gold nanostructures coated cellulose membrane for the quantification of uric acid in an aqueous solution. The synthesis of system was achieved by functionalizing cellulose membrane with poly[2-(methacryloyloxy)ethyl]trimethylammonium chloride (PMETAC) and cross-linked with ethylene glycol dimethacrylate (EGDMA). FT-IR and XPS confirm the formation of PMETAC and PMETAC/EGDMA on the cellulose. The fabricated substrates were exposed to tetrachloroaurate solution, then reduced by NaBH4. We have systematically investigated the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) in various pH conditions, absent uric acid, using the fabricated substrates. The colorimetric response—observed through UV–Vis spectroscopy—revealed significant shifts in absorbance at 660 nm, correlating with uric acid concentrations across a range of pH levels. The films exhibited a pronounced color change from green to light yellow in basic to neutral environments and from yellow to dark green under more acidic conditions, demonstrating their potential for high-sensitivity uric acid detection. The assessment of the catalytic films' reusability and stability revealed insights into their enduring performance, identifying opportunities for enhancing material design and functionality for extended applications. This study not only underscores the films' versatile detection capabilities but also emphasizes the importance of pH in tuning the assay's sensitivity and specificity.