Semipermanent Dyes Research Articles

Associations between use of chemical hair products and epigenetic age: Findings from the Sister Study

Background: Hair products may be a source of harmful chemicals and have been linked to age-related health outcomes. We investigated whether the use of hair products is related to epigenetic age in a sample of Black (both Hispanic and non-Hispanic) and non-Hispanic White women. Methods: In a subset of 4358 participants aged 35–74 years from the Sister Study, we estimated cross-sectional associations between self-reported use of four chemical hair products (permanent dye, semipermanent dye, straighteners/relaxers, and hair permanents/body waves) in the year before enrollment (2003–2009) and three DNA methylation-based measures of epigenetic age (DunedinPACE, GrimAge age acceleration [GrimAgeAccel], and PhenoAge age acceleration [PhenoAgeAccel]) using survey-weighted multivariable linear regressions. Associations were estimated both overall and by self-identified race and ethnicity, adjusting for chronological age, socioeconomic and lifestyle factors, body mass index, menopausal status, and DNA methylation platform. Results: Associations between the use of hair products and the three epigenetic age measures were largely null. Use of hair permanents/body waves was modestly associated with higher DunedinPACE among all participants (β ever-never = 0.010; 95% confidence interval [CI] = 0.001, 0.019) and with lower PhenoAgeAccel among Black women (β ever-never = −1.53; 95% CI = −2.84, −0.21). Conclusion: In this US-based study, we found little evidence of associations between chemical hair product use and epigenetic age in Black and non-Hispanic White women. Observed associations were modest and largely not supported by dose–response relationships or were inconsistent across epigenetic age measures. Previously observed associations between chemical hair product use and aging-related health outcomes may not be explained by the biological aging pathways captured by DunedinPACE, GrimAgeAccel, or PhenoAgeAccel. Alternative biological pathways are worth investigating in racially diverse samples.

Surface-enhanced Raman spectroscopy enables confirmatory detection of dyes on hair submerged in hypolimnion water for up to twelve weeks.

Difficulties in the localization of bodies of homicidal or drowning victims in natural water result in their submergence for weeks if not months. Water insects and microbes drastically change the body's appearance, which significantly changes the determination of a victim's identity. DNA analysis is commonly used for identifying the decedent; however, this PCR-based approach is time-consuming and destructive of the evidence. Considering that nearly half of the people in the world dye their hair with a variety of permanent and semi-permanent dyes, one can expect that confirmatory identification of dyes on the body's hair can be used to shed light on the victim's identity. A growing body of evidence suggests that surface-enhanced Raman spectroscopy (SERS) can be used to detect and identify hair dyes. In this study, we investigated the extent to which SERS could be used to detect black and blue, permanent and semi-permanent dyes on hair submerged in hypolimnion water for up to twelve weeks. We found that SERS enabled 100% accurate identification of analyzed dyes on hair submerged in hypolimnion water for up to 8 weeks, whereas, on average, 87% accurate identification of the hair dyes could be achieved on hair exposed for 10 weeks and 50% for hair exposed 12 weeks in hypolimnion water. We also found that the aqueous environment caused progressive fading of some dyes, whereas other dyes showed substantial spectral transformations after prolonged submergence. Finally, we found that changes in the intensity of vibrational bands of dyes could be used to predict the duration of submergence of colored hair in hypolimnion water.

Elucidation of the effect of heat exposure on hair colored by permanent and semipermanent colorants using surface‐enhanced Raman spectroscopy

Confirmatory identification of hair colorants can be used to establish a connection between a suspect and the crime science or demonstrate the absence of such connections. A growing body of evidence shows that surface-enhanced Raman spectroscopy (SERS) could be a confirmatory, minimally destructive, and fully noninvasive analysis of hair colorants. In SERS, a signal that provide the information about the chemical structure of both permanent and semipermanent dyes present on hair is enhanced by a million-fold using noble metal nanostructures. However, it is unclear whether the information of hair colorants can be revealed if hair was contaminated or exposed to harsh environments such as sunlight and heat. In this work, we determine the effect of a short- and long-term heat exposure on SERS-based analysis of hair colored with blue and red permanent and semipermanent dyes. We found that short and especially long-term heat exposure at 220°C could alter chemical structure, and consequently SERS spectra, of permanent and semipermanent colorants. This thermal degradation of permanent dyes complicates their direct identification using SERS. We also found that partial least squares discriminant analysis can be used to overcome this issue allowing for highly accurate identification of both permanent and semipermanent dyes on colored hair that was exposed to 220°C for 6-12 min. These results show that heat exposure of colored hair should be strongly considered upon their SERS-based examination to avoid both false positive or false negative identification of chemical dyes.

The effects of sun exposure on colorant identification of permanently and semi-permanently dyed hair

During bloating and active decay, human remains begin to deform and warp their physical identity. After the skin and muscles loosen and detach from their skeletal structuration, everything but bones, teeth, and hair will fully disintegrate into the soil that surrounds the body. Nearly half of people in the world dye their hair with a variety of permanent and semi-permanent colorants. Expanding upon this, we hypothesized that confirmatory analysis of hair colorants can be used to facilitate and advance forensic analysis of human remains. A growing body of evidence suggests that hair colorants can be identified directly on hair using surface-enhanced Raman spectroscopy (SERS). In this study, we investigate the extent to which SERS can be used to detect black and blue permanent and semi-permanent dyes on hair exposed to sunlight. Our results showed that although substantial photodegradation of all dyes was observed by week 7, SERS enabled highly accurate detection and identification of hair colorants during all 10 weeks of hair exposure to the sunlight with on average 99.2% accuracy. We also found that SERS could be used to predict fading rates of hair colorants. This information can shed light on the exposure of human remains to the exterior environment.

728 Chemical Burns and Eyebrow Tinting: An Unusual Case

IntroductionHere we present the case of chemical burns following professional eyebrow tinting, a phenomenon rarely described in the literature.MethodsA 50-year-old previously healthy female presented to our emergency department for evaluation and treatment of chemical burns to her eyebrows. Fifteen days prior, she underwent professional eyebrow tinting by a local esthetician. Blistering developed the following day, and progressive swelling prompted her to present to an outside hospital. There she was prescribed acyclovir, ketoconazole cream, and silver sulfadiazine, but despite these measures, her symptoms progressed, leading to her presentation to our facility. Both brows were remarkable for significant swelling with exudative crushing on the surface concerning for superficial infection of partial-thickness chemical burns. She was prescribed bacitracin, prednisone, and clindamycin and discharged with instructions to follow up in burn clinic. Six days later, both brows had developed 2x3 cm scabs, but no signs of infection were appreciated. She was instructed to apply bacitracin/polymyxin B ointment to the scabbing areas and open wounds. Forty-nine days after her tinting, both brows were noted to be completely healed with no alopecia.ResultsBurns following cosmetic procedures most commonly occur during hair lightening treatments where products frequently contain caustic chemicals such a hydrogen peroxide or persulphates leading to oxidation reactions lightening the hair. Thermal burns in hair salons have also been reported to heated hair-dressing instruments or external heat to hasten the highlighting process.The periorbital area is becoming an increasingly popular target for nonsurgical cosmetic procedures, including permanent eyelid tattooing, eyelash dying, and extensions, more recently, eyebrow tinting. Eyebrow tinting involves the application of semipermanent dyes to give the appearance of a fuller brow. There are no FDA-approved brow tinting formulations, and many contain para-phenylenediamine (PPD), a dying agent frequently associated with allergic dermatitis and less commonly chemical burns. This patient underwent brow tinting in a professional setting and still experienced a chemical burn. As the popularity of brow tinting increases, some patients will undoubtfully seek out readily available, unregulated products for self-application. The potential for dermatitis and chemical burns following eyebrow tinting will only increase.ConclusionsEyebrow tinting is not a benign cosmetic procedure, and even professional application of dyes may lead to chemical burns. We believe an increased awareness of brow tinting and its potential complications is warranted given its increasing popularity, the preponderance of unregulated products, and the potential for poor cosmetic outcomes.

A Study on the Safety Management Educational Plan for Hazardous Chemicals in Semi-Permanent Make-up Dyes

It is urgent to provide education against semi-permanent makeup cosmetologists to examine the hazard and safety of dyes. For this, various literature materials including domestic laws were analyzed, and previous studies were reviewed. First, the Ministry of Environment regulates chemical materials for tattoo dyes through the Safety & Labeling Standards for Household Chemical Products. Therefore, semi-permanent makeup training institutes are required to organize safety & health-theory curriculums and educate safety bureaus and related laws concerning semi-permanent dyes. Second, according to the Occupational Safety and Health Act of the Ministry of Employment and Labor, information about the hazard and handling methods of various chemicals is developed through the Material Safety Data Sheets(MSDS) system, and information service and training systems are established. Hence, there should be efforts to enhance awareness in cooperation with associations and training institutes such as promotion of the MSDS system. If specific training programs on the MSDS information utilization methods and systems are given, a united training system for household chemical products and occupational safety & health, not just acquisition of information on chemicals, can be established.

Influence of cosmetic hair treatments on hair of methamphetamine abuser: Bleaching, perming and coloring

Various cosmetic hair manipulations are known to interfere with the drug of abuse concentrations in hair. It is important to know the effects of cosmetic hair treatments as they may cause false-positive or false-negative results. The article aimed to investigate the effect of bleaching, perming, and dyeing treatment on the hair of methamphetamine（MA）abusers. The research results show that during the bleaching treatment, the content of MA in the hair is positively correlated with the degree of the treatment. Bleaching caused strong chemical degradation on MA, while perming exerted more a leaching out effect. Temporary dyes in single applications had only little effects on MA, Semi-permanent dyes can penetrate into the hair and exert a stronger matrix effect. The effect of permanent hair dye on the content of MA in hair mainly depends on the concentration of hydrogen peroxide.

New study links hair chemicals to breast cancer.

New study links hair chemicals to breast cancer.

Hair dye and chemical straightener use and breast cancer risk in a large US population of black and white women.

Many hair products contain endocrine-disrupting compounds and carcinogens potentially relevant to breast cancer. Products used predominately by black women may contain more hormonally-active compounds. In a national prospective cohort study, we examined the association between hair dye and chemical relaxer/straightener use and breast cancer risk by ethnicity. Sister Study participants (n = 46,709), women ages 35-74, were enrolled between 2003 and 2009, and had a sister with breast cancer but were breast cancer-free themselves. Enrollment questionnaires included past 12-month hair product use. Cox proportional hazards models estimated adjusted hazard ratios (HRs) and 95% confidence intervals (95% CIs) for the association between hair products and breast cancer; effect measure modification by ethnicity was evaluated. During follow-up (mean = 8.3 years), 2,794 breast cancers were identified. Fifty-five percent of participants reported using permanent dye at enrollment. Permanent dye use was associated with 45% higher breast cancer risk in black women (HR = 1.45, 95% CI: 1.10-1.90), and 7% higher risk in white women (HR = 1.07, 95% CI: 0.99-1.16; heterogeneity p = 0.04). Among all participants, personal straightener use was associated with breast cancer risk (HR = 1.18, 95% CI 0.99-1.41); with higher risk associated with increased frequency (p for trend = 0.02). Nonprofessional application of semipermanent dye (HR = 1.28, 95% CI 1.05-1.56) and straighteners (HR = 1.27, 95% CI 0.99-1.62) to others was associated with breast cancer risk. We observed a higher breast cancer risk associated with any straightener use and personal use of permanent dye, especially among black women. These results suggest that chemicals in hair products may play a role in breast carcinogenesis.

Surface-Enhanced Raman Analysis of Underlaying Colorants on Redyed Hair.

Forensic examination of hair evidence can help with establishing a connection between a suspect and a crime scene or demonstrate the absence of such connections. Currently, it is primarily done by a subjective microscopic examination which can only elucidate the species of origin and, if human, the part of the body the hair came from. Several years ago, surface-enhanced Raman spectroscopy (SERS) was proposed for advanced forensic analysis of hair ( Kurouski , D. ; Van Duyne , R. P. In situ detection and identification of hair dyes using surface-enhanced Raman spectroscopy (SERS) . Anal. Chem. 2015 , 87 , 2901 - 2906 . DOI: 10.1021/ac504405u ). It was shown that SERS could be used to determine whether hair was dyed or not and even reveal what commercial hair colorant was used. Expanding upon those findings, we show that SERS is capable of probing the original colorant even if hair was redyed afterward. Specifically, we were able to detect and identify the underlaying blue semipermanent colorant on hair redyed by both black semipermanent and black permanent colorants. We also demonstrate that original black permanent colorant could be detected by SERS if the hair was recolored by blue semipermanent dye. However, it could not if the hair was recolored by another (blue or black) permanent dye. We also provide experimental evidence that SERS can be used to detect the dye on hair colored more than two months prior to its spectroscopic examination. These experimental findings substantially expand capabilities of SERS in forensics.

Trends in use of hair Dye: A cross-sectional study

Objective:The objective of the following study was to assess the knowledge, practice, perception and adverse reactions among hair dye users.Settings and Design:A cross-sectional study was conducted on patients, their attenders and staff of SDUMC.Materials and Methods:A questionnaire (28) pertaining to use of hair dyes and reactions to them was administered randomly.Statistical Analysis:The demographic data are expressed as mean ± standard deviation, quantitative data expressed as percentages.Results:A total of 263 volunteers responded of which 52.5% and 47.5% were females and males respectively with mean age of 32.40 ± 6.01 years. The participants started using hair dye at an age of 27 (±3.63) years. Synthetic dyes were used by 91%. Frequency of use in a year was 2-5 times in 51%. Instruction of the package insert was followed by 63.5%. Skin test was never performed in 34% prior to their use. Nearly 96% expressed hair dyeing was unsafe during pregnancy, lactation and in children. Adverse reactions were reported by 42%, with headache (63%) and itching (38%) being the most common.Conclusion:Hair dyeing was started at very early age. Majority used semi-permanent dyes even though they perceived natural dyes were safe. They continued dyeing despite adverse reactions, which indicates cosmetic importance.

Use of hair colouring products and risk of multiple myeloma among US women

Objective: To evaluate the association between personal hair dye use and risk of multiple myeloma among women. Methods: A population-based case–control study of 175 cases of multiple myeloma and 679...

Understanding Dermal and Inhalation Exposure for Hairdressers Through Questionnaire, Diaries and Surveys

P-096 Introduction: A number of hairdressers suffer from occupational dermatitis and asthma related reactions as a result of harsh occupational chemical products. There is a need to evaluate and manage exposure practices in salons and old, new and emerging chemical products. The analyzed results of data collected from questionnaires, diaries, and surveys from a preliminary project of ten salons and ten hairdressers in the Little Rock Metropolitan area will be presented. Methods: In this study, hairdressers were required to maintain a five-day diary of treatment procedures, the number of daily clients, and any personal protective equipment used. Hairdressers were also required to answer a 60 minute questionnaire covering demographics, product usage, personal care, workplace practices, protective equipment, safety training, workplace ergonomics, general health, and risk perception. Salon owners were also required to answer a 60 minute questionnaire on demographics, salon characteristics, product purchases, workplace practices and ventilation. Researchers also conducted a short walk-through survey/sketch to identify the location of HVAC, exhaust systems and overall layout. Results: Analyzed results across the salons and across hairdressers indicate that there is much variability for these hairdressers, especially in the types and brands of products used and in the number of clients seen each day (approx 1–10 clients, over 200 different product brands). This results in highly variable exposures. A range of 30–200 minutes a day is spent using harsh hair care treatments on clients (e.g., streaking, permanent and semi-permanent dyes, and relaxers). Those that mostly preformed these harsher hair care treatments complained of sinus problems. Consistently, gloves were used by all during the application of strong dyes and relaxers but were not used during washing and styling. Face masks were never used and exhaust fans were found in only two of the salons. Face mask and exhaust fans can protect against inhalation exposure. A number of other summarized findings from the questionnaires, diaries and surveys will be discussed. Discussion: This preliminary research will aid in designing more extended monitoring for this population. The overall project also involves the use of tailored videotaping and translation methods to collect and describe the intermittent dermal (i.e., changing frequency and duration of contact with hair care products), and inhalation activities (i.e., frequency and duration in specific micro-environments within a salon's facility) leading to chemical exposures in the workplace. These micro-activities will later be compared to the activities gathered through the questionnaires, diaries and surveys.

West Coast study of childhood brain tumours and maternal use of hair-colouring products

The immature nervous system of the fetus is characterised by rapid cell growth and division and is particularly vulnerable to carcinogens and mutagens. Several epidemiological studies have reported an increased risk for childhood brain tumours (CBT) associated with exposure to N-nitroso compounds (NOC). Hair-colouring products (hair "dyes") that contain NOC-related aromatic amines have shown mutagenicity in vitro and carcinogenic properties in vivo. The potential public health impact of the relationship between hair dye use and carcinogenesis has prompted epidemiological research, given that a large proportion of American women have used hair dyes. A large population-based case-control study was conducted on the west coast of the USA to investigate risk factors for CBT including exposure to NOC. Eligible CBT patients (<20 years of age and diagnosed between 1984 and 1991) were identified from cancer registries in Los Angeles County, the San Francisco Bay Area in California and the Seattle area in Washington state. A total of 540 biological mothers of these children were interviewed, and 801 control subjects who were frequency matched to the CBT patients on birth year and sex were obtained using random digit dialling. Mothers were asked details about personal use of hair dyes during the index pregnancy including frequency of use, trimester of use and type of dye used. Results from age- and sex-adjusted unconditional logistic regression analyses showed no association between risk for CBT and use of hair dyes 1 month before and/or during pregnancy nor during specific trimesters. A nearly twofold increased risk for CBT was associated with single-interval use during the 1 month before pregnancy, but the confidence interval (CI) was imprecise and the estimate was not different from unity (OR = 1.9, 95% CI [0.5, 7.0]). Exclusive use of permanent dye, temporary dye or hair darkeners was not associated with risk for CBT. A twofold increased risk (OR = 2.0, 95% CI [0.83, 4.7]) was observed with exclusive use of semi-permanent dye during the month before or during pregnancy. Exclusive use of semi-permanent dye during the month before pregnancy and/or first trimester also was associated with an elevated risk for CBT, again not different from unity and with an imprecise CI (OR = 2.5, 95% CI = [0.58, 10.3]). There was no evidence of an association between risk for CBT by histological subtypes and use of hair dyes during the index pregnancy or the month before conception. Together with results from previous studies, these results provide no consistent evidence of an association between risk for CBT and use of hair dyes during pregnancy.

Application of TLC and HPTLC in the analysis of semipermanent hair dyes

Synopsis Semipermanent or direct colouring includes any product capable of affecting to some extent a change in the natural hair colour that will last through at least five shampoo washings. Semipermanent dyes are simple and easy to use, as opposed to oxidation dyes, and are normally formulated for application on nonbleached hair. Following increases in supply of such formulations, we have started an analysis for quality control purposes of 21 commonly marketed dyestuffs (nitroaminobenzenes, anthraquinone and Arianor dyes) and 20 colouring products manufactured by four leading companies. By using TLC (silica gel and reversed phase) and HPTLC (silica gel) procedures we have determined relative retention values to 1,3-diamino-4-nitrobenzene of standards and dyes found in the commercial products. All the values reported (standards and samples) are the average of five analytical results (+/-SD).

Case-Control Study of Hair Dye Use by Patients With Breast Cancer and Endometrial Cancer&lt;xref ref-type="fn" rid="FN2"&gt;2&lt;/xref&gt;

A case-control study was undertaken of use of permanent and semipermanent hair dyes by women with cancers of several sites, including breast and endometrium. In London, Ontario, 50 cases of of breast cancer and in Toronto 35 cases of breast cancer and 36 cases of endometrial cancer were identified in cancer treatment centers. In London, controls were selected from hospitalized women with diseases other than cancer; in Toronto, controls were selected from women living in the same neighborhood as the patients with cancer. The results did not suggest an increased risk of either breast or endometrial cancer in users of permanent or permanent and semipermanent dyes combined. Although the numbers of cases and controls were small, the consistency of the results for both sites, in both study centers, and the absence of any clear positive relationship between various measures of intensity of use and risk of cancer provided evidence that a large increase in risk was not missed.