Sometimes we need to minimize the conditions of topology for different reasons such as obtaining more convenient structures to describe some real-life problems, or constructing some counterexamples whom show the interrelations between certain topological concepts, or preserving some properties under fewer conditions of those on topology. To contribute this research area, in this paper, we establish some new concepts on supra topological spaces using supra semi-open sets and give some characterizations of them. First, we introduce a concept of supra semi limit points of a set and study main properties, in particular, on the spaces that possess the difference property. Second, we define and investigate new separation axioms, namely supra semi Ti-spaces (i = 0, 1, 2, 3, 4) and give complete descriptions for each one of them. We provide some examples to show the relationships between them as well as with STi-space.