Semi-natural Habitats Research Articles

Assessing the EU27 Potential to Meet the Nature Restoration Law Targets.

The Nature Restoration Law adopted by the European Union in 2024 aims to implement measures to restore at least 20% of its land and sea by 2030 and all ecosystems in need of restoration by 2050, focusing on among others agricultural land, forests, urban, marine, freshwater, and wetlands areas. The goal is to enhance the natural and semi-natural habitats' role in achieving climate targets and preserving biodiversity. Member States must submit detailed national restoration plans, outlining specific actions and mechanisms for monitoring progress. However, these plans should align with the ongoing Common Agricultural Policy and National Energy and Climate Plans objectives. Using data from European Commission reports and applying a semantic interval scale methodology, this study quantifies each Member State's ambitions and effectiveness under the National Energy Climate Plans and Common Agricultural Policy and establishes a benchmark for reporting under the Nature Restoration Law. The findings reveal the National Energy Climate Plans' wide disparities in implementing decarbonization measures, climate change adaptation and the implementation of nature-based solutions. The Common Agricultural Policy Plans exhibit only partial commitment to greening agriculture, yet their alignment with Nature Restoration Law objectives varies. Therefore, timely coordination between the three strategies is crucial to avoid conflicting goals, overlapping efforts, and wasting time and resources, ensuring the success of restoration actions.

The protection planning of small and micro habitats in urban fringe areas: Enlightenment from the management of high natural value farmland in the EU to the maintenance of bio-diversity in urban areas in China

<p>The habitat unit in urban fringe is an important link to support regional biodiversity; due to the influence of urban system and agricultural system, most of them are semi natural, appeared with diverse types, small patches, scattered layout, and generally relying on low intensity farming activities. Export-oriented expansion of urban construction land and intensive agricultural production are prone to reduce the scale and quality of semi natural habitats in urban fringe areas; and the existing measures cannot effectively protect them. High natural value farmland management is an effective measure of the EU to protect semi-natural habitats related to agriculture and maintain regional biodiversity. Through combing the main points of high natural value farmland such as type definition, evaluation, identification, maintenance management, etc., this paper proposes the suggestions for optimization of small and micro semi-natural habitat protection in China: (1) strengthening the recognition of the value of small and microhabitats and related land use; (2) identifying small and micro-habitats that are conducive to the maintenance of biodiversity, and incorporating them in the overall protection of regional network; (3) combining the rigidity and flexibility of land use control, with the consideration of complex function demands; (4) Converging with the existing statutory planning to enhance the protection efficiency of small and micro habitats.</p>

Economic efficiency of nature-based solutions: Theoretical framework and application to semi-natural habitat implementation.

The application of nature-based solutions to agriculture is promising because it allows the sustainable management of ecosystems and the reconciling of human well-being with the benefits of biodiversity. However, scientists lack robust economic arguments and concepts in the area of nature-based solutions that are well aligned with the expectations of the agricultural sector. This study addresses this gap by developing an interdisciplinary economic framework that integrates nature-based solutions and allows for an assessment of their efficient use. The conceptual framework is derived from a standard agricultural production model, making it possible to determine optimal levels of use and value. The framework is then applied to the establishment of semi-natural habitats, using econometric calibration and simulations based on agronomic, economic, psychological, and ecological data from a single case study in western France. The estimations show that the optimal semi-natural habitat coverage rate was 17.5% which was half the observed level, suggesting an underuse according to this framework. The present framework, which builds on standard economic efficiency models, demonstrates that ecosystems contribute to production similarly to conventional agricultural inputs, providing a productivity-based justification for their conservation.

Unveiling the effects of land use and intra-seasonal variation on bee and plant diversity and their ecological interactions in vegetation surrounding coffee plantations

Land use, local management, and seasonal variation significantly impact the ecological dynamics of bee–plant communities and their ecological interactions. These variables negatively affect diversity and ecological interaction networks within human-dominated landscapes. Additionally, seasonal variables such as temperature, rainfall, and resource availability across different seasons play essential roles in shaping bee communities and their interactions with flowering plants. However, little is known about how diversity and ecological interaction networks of non-crop plants in agricultural landscapes respond to intra-seasonal variations, specifically within the rainy season. In this study, we assessed how land use types, coffee crop management, and intra-seasonal variation within the rainy season influenced the composition and diversity of bee and plant communities, and their interaction networks in semi-natural habitats surrounding coffee plantations. We recorded the diversity of bees and plants and analysed their interactions networks metrics, such as specialisation, nestedness, modularity, connectance and bee/plant generality, in 8 pairs of sites. Our findings indicate that human settlements negatively influence bee generality, suggesting that human-dominated land and the introduction of exotic plants reduce floral resources for bees, which may decrease bee visitation. In contrast, extensive semi-natural and forested areas seemed to support bee generality. Additionally, we observed higher visit frequency and richness of bees and plant generality during the second period of the rainy season (July to October), leading to more robust bee–plant interaction networks in the same period. This study enhances our understanding of how land-use types and intra-seasonal climatic variation shape structure of bee floral visitor communities and their interactions with flowering plants. Furthermore, our findings underline the negative impact of human-dominated landscapes on the ecological dynamics of plants visited by bees and their interaction networks.

Capturing the effects of flower strips on natural pest control in agronomic land use models

ContextGiven policy objectives for pesticide reduction, functional biodiversity is increasingly important for agricultural production. However, economic land use optimisation models rarely account for the interplay between agricultural production and functional biodiversity, like natural pest control (NPC). ObjectiveWe present and discuss an approach to consider feedback effects between NPC and agricultural production in a geodata-based farm economic land use model. MethodsThe geodata-based land use model PALUD was extended to incorporate interactions between NPC and crop yields. This included linking a generic NPC model, based on semi-natural habitats, with an econometric approach to estimate yield gaps as a function of NPC potential. As a case study, the model was then applied to the German state Brandenburg, using permanent flower strips as example to promote NPC. Here, we analysed the impacts of enhancing NPC potential on food production and gross margins from arable land use. Results and conclusionsOur analysis revealed that a higher level of NPC through flower strips improved gross margins. by up to 6.4 % and crop production by up to 1.2 % within a pesticide-free environment in Brandenburg. Despite inherent limitations and uncertainties of the approach, our study provides a pivotal advancement in integrating NPC into economic land use models. Future refinements incorporating more detailed knowledge on context-specific interactions between crops, pests and predators will improve model robustness. Our approach and case study results provide valuable insights for policy-making on tailored sustainable landscape development and serve as a foundation for future modelling efforts. SignificanceTo our knowledge the interaction between NPC and crop yields has not yet been integrated into any geospatially based economic land use model before. Incorparating these interactions is vital for guiding policies towards sustainable agricultural systems. In this respect it is important to develop and discuss further methodological approaches.

Agricultural irrigation development in Castilla y León (Spain): driving forces and outcomes for landscape and sustainability in the 21st century

ContextAgriculture relies on irrigation in many parts of the world, and the need for irrigation is increasing due to rising demands for agricultural products and climate change-induced alterations in rainfall patterns. However, irrigated agriculture has been found to damage ecosystems and threaten landscape sustainability.ObjectivesAgainst this background, there has been a recent development towards large-scale irrigation in Spain. The aim of this study is to understand this development at the landscape level and its impact in the context of landscape sustainability.MethodsWe focused on two study sites in Castilla y León using a mixed-methods approach. We studied driving forces, landscape changes, and sustainability outcomes through document analysis, interviews, and aerial photograph analysis.ResultsThe development of a landscape-level underground pipe network took place at one study site and is planned for the second study site. Interviewees perceived institutional and social driving forces as particularly influential and technological driving forces as less influential. Political and economic driving forces were often interlinked. The irrigation development tied to land consolidation led to landscape changes, such as the removal of trees and increases in field size. Thus, in terms of environmental sustainability, trade-offs were found, while social sustainability outcomes were mainly positive. The impact on farmers’ economic security varied.ConclusionsFor further landscape-level irrigation developments, we recommend integrating preserving seminatural habitats and the structural diversity of the agricultural landscape in planning processes. We also recommend a shift towards more water efficient crops, evapotranspiration management, and a new funding scheme for farmers to offset rising electricity costs.

Semi-natural habitats are key to breeding bird diversity in intensified vineyard landscapes across Europe

In climatically suitable regions across Europe, vineyards can be the dominant perennial crop type. While many wine-growing landscapes are intensively managed, they may still be an attractive habitat for a wide range of bird species. In this study we investigated how breeding birds in three wine-growing regions in Europe (Germany: Palatinate, France: Bordeaux, Austria: Leithaberg) are influenced by the composition of the landscape, focussing on woody semi-natural vegetation.We recorded bird vocalizations with autonomous sound recorders in 93 landscapes across Europe. Bird species were identified according to their songs and calls. The landscape in a 200-m buffer around the recording points was mapped. In total, we recorded 72 bird species, including species typical for vineyard landscapes such as cirl bunting (Emberiza cirlus), hoopoe (Upupa epops) and turtle dove (Streptopelia turtur). For all three countries we found that an increase in overall woody vegetation in the landscape led to an increase in species richness and altered community composition. Most species were recorded in landscapes with abundant hedges, small woods and tree rows rather than in vineyard-dominated landscapes but e.g. woodlark (Lullula arborea) and linnet (Linaria cannabina) showed an opposite preference.We conclude that in intensively used wine-growing landscapes the ongoing decline in farmland birds and the ecosystem services they provide can be reversed by the reintroduction of semi-natural woody vegetation between vineyards. These in frequently many cases linear structural elements can be established included in the landscape with only small losses in production area.

Short‐Term Grasslands in Agriculture Support Both Natural Enemy and Phytophagous Arthropod Populations

ABSTRACTSimplified agricultural land with high chemical input is increasingly replacing natural habitats in many parts of the world. This loss and fragmentation of natural areas may have profound impacts on an array of wildlife, including predatory arthropods that provide natural biocontrol of agricultural pests. There is increasing interest in utilising short‐term seminatural habitat restoration in agricultural systems to support populations of beneficial organisms. We assessed the impact of two types of short‐term set‐asides on the diversity and abundance/activity density of phytophagous and predatory arthropods. We found that flower‐enhanced set‐asides supported higher abundances of both phytophagous and predatory arthropods compared to control crop fields. In addition, we found that both set‐aside types (flower enhanced and traditional) supported a more active and diverse community of beneficial predatory carabid beetles, with particularly strong trends in set‐asides supplemented with flowering plants. These results suggest that small‐scale, temporary, seminatural habitats in agricultural systems provide support for a diverse and abundant community of both beneficial and phytophagous arthropods.

Landscape heterogeneity and a competing herbivore reduce olive fruit infestation by the pest Prays oleae

Monocultural agricultural systems can favour arthropod pests through direct effects, such as promoting pest dispersion, and indirect effects, such as limiting natural pest control. Therefore, promoting the presence of non-crop habitats in agricultural landscapes has the potential to reduce pest damage. However, likely in part due to the trophic complexity surrounding arthropod pests, these effects vary strongly among different pests and crops, and they are rarely consistent on a year-to-year basis. Here, we formulated Generalized Additive Mixed Models with data of 11 years from 25 olive orchards from southern Spain to evaluate the effects of landscape composition at different spatial scales on fruit infestation by the moth Prays oleae, a key Mediterranean olive pest. Then, we evaluated whether these effects were modulated by natural enemies or heterospecific herbivores. Fruit infestation by P. oleae was affected by landscape composition at small spatial scales (100 m radius), but not at medium or large scales (500 m and 1000 m radii). Fruit infestation was negatively affected by the proportion of semi-natural habitats, while it was positively affected by the proportion of olive groves in the landscape. We did not find evidence that these effects were modulated by natural enemies. However, fruit infestation by P. oleae was negatively affected by the presence of Euphyllura olivina, which is considered a secondary olive pest. Overall, this study shows that the negative response of P. oleae to habitat heterogeneity is consistent across years, but these effects were found only at smaller spatial scales. Therefore, we recommend implementing patches of semi-natural vegetation within olive groves as it can help reduce crop damage caused by P. oleae. Specifically, our models showed that maintaining 25 % of non-crop vegetation within 100 m radius buffer zones would reduce by half the proportion of fruits infested by P. oleae. Interestingly, this study also suggests that the secondary pest E. olivina can prevent fruit infestation by P. oleae, which in turn could increase crop yield.

Multi-Annual Study of Eriogaster catax (Linnaeus, 1758) (Lepidoptera, Lasiocampidae) Oviposition Strategy in Transylvania's Largest Population: Key Insights for Species Conservation and Local Land Management.

This study provides new insights into the oviposition strategy of Eriogaster catax (Linnaeus, 1758) (Lepidoptera, Lasiocampidae), an endangered species of moth found in semi-natural habitats within agricultural landscapes. Protected under various European directives and listed as Data Deficient by the IUCN, E. catax inhabits warmer regions of the Western Palearctic. Despite noted geographic variations in its ecological preferences, few studies have statistically significant data on its ecology. Our six-year study, conducted within the largest known population of E. catax. in Romania, reveals critical data on its oviposition preferences, including the species' tendency to utilize Prunus spinosa L. and Crataegus monogyna Jacq. shrubs at an average height of 80.48 ± 34.3 cm, with most nests placed within the 41-80 cm range and containing an average of 186 ± 22 eggs. The study also addresses the species' vulnerability to human activities such as bush trimming, agricultural burning, and uncontrolled grazing, particularly due to its low oviposition height. These findings underscore the negative impact of overgrazing and burning practices, particularly when conducted on a large scale, on the conservation of E. catax. The detailed ecological requirements identified in this study are essential for developing effective conservation strategies and habitat management practices. Furthermore, the study highlights the importance of local community involvement and public education in raising awareness about biodiversity and the conservation of endangered species.

A New Record of Elymnias Caudata Butler, 1871 (Insecta: Lepidoptera: Nymphalidae) from Nagpur in Central India

Urban areas harbour diverse butterfly species in isolated and fragmented habitats such as parks, remnants of natural and semi-natural habitats and other such green areas. An Indian endemic nymphalid butterfly species, Elymnias caudata Butler, 1871 (tailed palmfly), has mostly been reported from south-western parts of the southern region of India. According to the previous record, this species is unknown from Nagpur, a city in Central India. Here, Elymnias caudata is recorded from Nagpur for the first time, and it shows that the distribution range of this species is wider than previously known. This is the northernmost record of Elymnias caudata in India.

Effects of sown and spontaneous inter-row vegetation on weeds and beneficial arthropods in vineyards

The intensification of crop management has resulted in a decline of biodiversity in the last decades, in particular through habitat loss, fragmentation and degradation. Semi-natural habitats within agricultural landscapes such as hedges, grasslands or herbaceous field margins, provide resources and refuge to beneficial arthropods. In vineyards, extensively used inter-row vegetation may be functionally equivalent to such semi-natural habitats, and sowing of plant species rich in floral resources may improve habitat functions. In this study, three types of vineyard inter-row vegetation treatments were compared in 15 vineyards of South-eastern France: (1) sowing a high-diversity seed mixture (HD) with a high number of nectariferous plant species, (2) spontaneous vegetation, and (3) tilled inter-rows. We monitored the inter-row vegetation including problematic weeds, the abundance of beneficial arthropods, and the predation of sentinel prey. The invasive weedy grass species Cynodon dactylon showed a lower cover in HD inter-rows than in spontaneous vegetation, whereas no differences were found for the invasive herb Erigeron sumatrensis. Both weed species were still best controlled in tilled inter-rows. Beneficial arthropods were less abundant in tilled than in spontaneously vegetated and sown inter-rows. Day predation was higher in HD inter-rows than in spontaneous vegetation although no significant differences were found for observed predators. Over all treatments, plant species richness, flower and grass cover had a positive influence on several beneficial arthropod groups. Our results highlight the positive effects of species-rich inter-row vegetation on weed control, beneficial arthropod abundance and predation but also showed that further research is needed to improve the efficiency beyond services already provided by spontaneous vegetation.

Wild Bee Conservation in Viticulture: Effects of Semi-Natural Habitats, Organic Management, and Fungicide Reduction.

In agricultural landscapes, the removal of semi-natural habitats (SNH) and the intensive use of pesticides contribute to declines of biodiversity, including crop pollinators such as bees (Hymenoptera: Apoidea). However, effects of pesticide use and landscape characteristics on bees have rarely been studied together. In this study, we investigated how SNH in the surrounding landscape, organic and conventional management, and the reduction of fungicides affect wild bee diversity in 32 vineyards in southwest Germany. We used yellow pan traps to sample bees in a crossed design of management (organic vs. conventional) and fungicide use (reduced in fungus-resistant grape varieties vs. regular) along a gradient with increasing proportions of SNH in the surrounding landscape. Higher proportions of SNH influenced species composition of bees and increased the abundance and richness of above-ground-nesting species. Organic vineyards had a 49% higher abundance of bees compared to conventional vineyards. The reduction of fungicides did not affect bee diversity nor abundance. The absence of a response to fungicide intensity suggests that the benefit of organically managed vineyards to wild bees was through differences in their vegetation management, which is in line with the positive response of bees to SNH in the surrounding landscape. Synthesis and applications: Our study underlines that the local provision of diverse vegetation in vineyards and the landscape-scale provision of suitable SNH are key factors for wild bee conservation in viticulture.

Assessment of Common Ragweed (Ambrosia Artemisiifolia L.) Seed Predation in Crop Fields and Their Adjacent Semi-Natural Habitats in Hungary

Ambrosia artemisiifolia has turned into a noxious weed species in agricultural fields and landscapes in Europe. Durable control options are still needed to limit the abundance of this species. Weed seed consumption by naturally occurring seed predators is a key ecosystem service in agricultural areas. Seed predation levels of common ragweed were examined in wheat and maize fields and adjacent semi-natural habitats (SNHs). To evaluate the weed seeds’ exposure to invertebrate seed predators, 20 cards each were set on the soil surface inside the crop field and in SNHs with four replications. Twenty seeds of ragweed were attached to sandpaper. Seed removal was assessed every 24 h of exposure for 5 days in June and November 2019, October 2020, and June 2021. The seed consumption level was measured according to the number of removed seeds from the seed cards. High consumption rates of ragweed seeds were found in all sampling rounds in both seasons and habitats. The seed predation rates in 2019 were stronger within crop fields in summer than in autumn with a slight difference between SNHs and inside fields. Our results demonstrate the possibility of seed predation contributing to Integrated Plant Protection (IPM) of common ragweed in rural areas.

The role of wild bees and cavity-nesting wasps as ecological indicators of the last traditionally managed meadows in Eastern Europe.

The number of wild bees and cavity-nesting wasps is abundant in agricultural areas and they contribute significantly to ecosystem services. Due to their specialization in nesting sites and food sources, these groups are sensitive to habitat condition changes and they are therefore important indicators for environmental impact assessments. As semi-natural habitats are steadily declining and often understudied, their significance for research is increasingly recognized. During this research, the role of wild bee species and cavity-nesting Hymenopteran taxa as indicators was examined, along the unique combination of high nature value and traditional land use habitats in Eastern Europe, Transylvania. Transects and trap nests were used to test the diversity and abundance of wild bees and cavity-nesting Hymenopterans to identify possible differences between highly protected and less protected areas. The differences in taxonomic groups between the sites and the potential effects of landscape structure on wild bees and cavity-nesting Hymenopterans were also assessed. We detected a high diversity of wild bee species and a significant species replacement from one study year to another. Among the nest-building Hymenopteran taxa, the majority of nests was built by Trypoxylon sp. during both study years, with a stronger dominance in the second year. The different taxonomic groups of wild bees and cavity-nesting Hymenopterans showed differences in their habitat affinities. The majority of the sampled bumblebee species as well as Trypoxylon sp. had an affinity towards the study sites located within the highly protected study area. Altogether, we found different habitat preferences for different Hymenopteran groups (both wild bees and wasps) and conclude that these groups definitely have the potential to serve as indicators for differences in the intensity of land use.

Remote sensing reveals the importance of seminatural habitat and irrigation on aphid biocontrol in arid agroecosystems

Abstract Remote sensing and spatial analyses are increasingly used to understand the role of seminatural habitats in biocontrol, but knowing how to best leverage these tools is a persistent challenge. In arid agroecosystems, irrigation often exaggerates the differences between crop fields and their interspaces. Weeds adjacent to irrigated fields are thus often some of the only persistent vegetation. Remote sensing may be essential to capture the effect of this fine‐scale variation on pest biocontrol. We examined the role of land‐cover in determining the degree of aphid pest pressure and biocontrol in irrigated alfalfa fields in the Great Basin Desert (Nevada, USA). We experimentally manipulated predator densities to determine true agents of aphid biocontrol in this system. We then assessed land‐cover and spatial scale using alternative methods to determine the approaches that best defined the combined direct and indirect effects of seminatural habitat on aphid pest suppression. A remote‐sensing approach revealed the importance of land‐cover to aphid biocontrol more clearly than conventional vegetation surveys. In particular, remote sensing revealed that weedy patches within alfalfa fields reduced aphid densities, whereas flood irrigation increased aphid densities. Our experiments confirmed that coccinellid beetles are important aphid biocontrol agents. Coccinellids were positively associated with weedy land‐cover that was spatially close to alfalfa fields, particularly in the spring. Other predator taxa were also associated with distinct landscape features, though these associations varied between spring and fall. Solution: Weedy seminatural habitat near alfalfa fields, occurring along field margins and the banks of irrigation ditches, was positively associated with the density of coccinellid beetles and associated aphid pest suppression. Remote sensing excelled at identifying these areas and their effects, highlighting its utility as a management tool. Preserving these weedy areas can enhance aphid biocontrol, but farmers must consider potential trade‐offs between the control of insect‐pests and weeds.

Gardens reduce seasonal hunger gaps for farmland pollinators.

Gardens can benefit pollinators living in surrounding farmland landscapes, but the reason for their value is not clear. Gardens are no different from many semi-natural farmland habitats in terms of the quantity of floral resources (pollen and nectar) they produce, but the timing of their resource supply is very different, which may explain their value. We show that gardens provide 15% of overall annual nectar in farmland landscapes in Southwest UK, but between 50% and 95% during early spring and late summer when farmland supplies are low. Gardens can therefore reduce seasonal nectar gaps experienced by farmland bumblebees. Consistent with this pattern, bumblebee activity increased in gardens relative to farmland during early spring and late summer. An agent-based model reinforces this point, showing that timing, not quantity, of garden nectar supply enhances bumblebee colony growth and survival in farmland. We show that over 90% of farmland in Great Britain is within 1 km of a garden and therefore positive actions by gardeners could have widespread spillover benefits for pollinators across the country. Given the widespread distribution of gardens around the world, we highlight their important interplay with surrounding landscapes for pollinator ecology and conservation.

Insect conservation in agricultural landscapes needs both high crop heterogeneity and semi-natural habitats

Identifying landscapes that are suitable for both biodiversity conservation and agricultural production is a major challenge. Traditionally, much research has focused on biodiversity conservation outside of agricultural production areas, e.g., in semi-natural habitats. In contrast, recent research has mainly focused on the potential of crop heterogeneity. This includes both compositional (crop diversity) and configurational heterogeneity (field border density). However, if and how crop heterogeneity, and semi-natural habitats interact to shape insect diversity in agricultural landscapes remains poorly understood. Here we investigated the combined effects of crop diversity, field border density, and semi-natural habitats (i.e., grassland proportion, hedge density) on insect diversity. We sampled insect communities from 14 – 17 June 2021 with pan traps in 27 study landscapes (500 m x 500 m) covering independent gradients of these landscape variables and identified a total of 587 insect species with DNA metabarcoding. We found that field border density mediated the effects of crop diversity, grassland proportion, and hedge density on insect richness. At low levels of field border density (i.e., landscapes with mostly large fields), effects were either neutral (crop diversity), negative (grassland proportion) or weakly positive (hedge density). By contrast, at high levels of field border density, crop diversity, grassland proportion, and hedge density all exerted positive effects on insect richness. Responses to crop heterogeneity and semi-natural habitat differed among trophic groups of insects (decomposers, herbivores, parasitoids, predators). While variation in richness of herbivorous insects followed the patterns of the overall richness, decomposer richness was not related to any of the investigated variables. Predator richness increased with hedge density in landscapes, whereas parasitoid richness increased when high levels of field border density and grassland proportion coincided. Our study shows that increasing crop heterogeneity is a viable strategy for promoting insect diversity in agricultural landscapes. However, the effects of the amount of remaining semi-natural habitats, such as grassland or hedges, are mediated by configurational heterogeneity, and vary between trophic groups. Efforts to conserve insects in agricultural landscapes must therefore focus on both increasing the heterogeneity of the crop matrix by promoting crop diversity and increasing the density of field borders, while also maintaining or restoring semi-natural habitats as important source habitats for insect species.

Serotonin drives aggression and social behaviors of laboratory male mice in a semi-natural environment.

Aggression is an adaptive social behavior crucial for the stability and prosperity of social groups. When uncontrolled, aggression leads to pathological violence that disrupts group structure and individual wellbeing. The comorbidity of uncontrolled aggression across different psychopathologies makes it a potential endophenotype of mental disorders with the same neurobiological substrates. Serotonin plays a critical role in regulating impulsive and aggressive behaviors. Mice lacking in brain serotonin, due to the ablation of tryptophan hydroxylase 2 (TPH2), the rate-limiting enzyme in serotonin synthesis, could serve as a potential model for studying pathological aggression. Home cage monitoring allows for the continuous observation and quantification of social and non-social behaviors in group-housed, freely-moving mice. Using an ethological approach, we investigated the impact of central serotonin ablation on the everyday expression of social and non-social behaviors and their correlations in undisturbed, group-living Tph2-deficient and wildtype mice. By training a machine learning algorithm on behavioral time series, "allogrooming", "struggling at feeder", and "eating" emerged as key behaviors dissociating one genotype from the other. Although Tph2-deficient mice exhibited characteristics of pathological aggression and reduced communication compared to wildtype animals, they still demonstrated affiliative huddle behaviors to normal levels. Altogether, such a distinct and dynamic phenotype of Tph2-deficient mice influenced the group's structure and the subsequent development of its hierarchical organization. These aspects were analyzed using social network analysis and the Glicko rating methods. This study demonstrates the importance of the ethological approach for understanding the global impact of pathological aggression on various aspects of life, both at the individual and group levels. Home cage monitoring allows the observation of the natural behaviors of mice in a semi-natural habitat, providing an accurate representation of real-world phenomena and pathological mechanisms. The results of this study provide insights into the neurobiological substrate of pathological aggression and its potential role in complex brain disorders.

Patch shape, connectivity and functional groups moderate the responses of bird diversity to agricultural diversification — A meta-analysis

Agricultural expansion and intensification pose significant threats to global biodiversity. However, the conservation of agricultural biodiversity is essential for harmonizing agricultural production with ecological sustainability. Agricultural diversification can mitigate the negative impacts of agriculture on ecosystem functioning and biodiversity. Characteristics of the broader landscape may also play an important moderating role in diversified agricultural landscapes, but are not fully solved. To bridge this gap, we conducted a meta-analysis of peer-reviewed studies to compare bird abundance and species richness in diversified agricultural landscapes compared to those in simplified farmlands and natural landscapes. We also explored the moderating role of species functional groups and 11 metrics of landscape composition, configuration and heterogeneity. We found a generally positive effect of perimeter-to-area ratio of farmland patches, connectivity within farmland patches, and connectivity within patches of natural and semi-natural habitats on bird abundance and species richness. Responses to other landscape metrics were less consistent. For pollinators, the abundance was higher in diversified agricultural landscapes compared to control groups, and particularly when compared to natural landscapes. For natural enemies, the species richness was significantly lower in diversified agricultural landscapes than in natural landscapes, while their abundance was not. The overall abundance and species richness of granivores and frugivores in diversified agricultural landscapes were comparable to those in control landscapes. These results indicated landscape type of control groups, species functional groups, and their interactions had consistent impacts on the responses of bird diversity. We found a vital role of farmland patch shape and connectivity within farmland and habitat patches for bird conservation in diversified agricultural landscapes, which has been rarely reported in previous studies. These insights underscore the need for policymakers and managers to develop farmland management and planning strategies at multiple scales to fulfill growing human demands while achieving biodiversity conservation objectives.