Seminal Fluid Of Patients Research Articles

Isolation of Circulating Tumor Cells from Seminal Fluid of Patients with Prostate Cancer Using Inertial Microfluidics.

Simple SummaryProstate cancer (PCa) is notoriously difficult to diagnose owing to the lack of reliable biomarkers and the invasiveness of obtaining a tissue biopsy from the prostate. As an alternative, we developed a liquid biopsy technique, based on isolating tumor cells from semen samples via a microfluidic device. To optimize the device, we first attempted to recover PCa cells from semen samples spiked with PCa cell lines, achieving an average efficiency of >87% cell recovery at the chosen flow rate. We then transitioned to a clinical setting using semen samples from PCa patients. The yield of isolated clinical PCa cells varied between 67 and 307 cells per mL of semen (in 15 cancer patients). These cells were stained and compared to the standard prognostic parameters such as Gleason score and PSA serum level. This study presents a potential liquid biopsy technique to augment the existing diagnosis and prognosis of PCa.Prostate cancer (PCa) diagnosis is primarily based on prostate-specific antigen (PSA) testing and prostate tissue biopsies. However, PSA testing has relatively low specificity, while tissue biopsies are highly invasive and have relatively low sensitivity at early stages of PCa. As an alternative, we developed a technique of liquid biopsy, based on isolation of circulating tumor cells (CTCs) from seminal fluid (SF). The recovery of PCa cells from SF was demonstrated using PCa cell lines, achieving an efficiency and throughput as high as 89% (±3.8%) and 1.7 mL min−1, respectively, while 99% (±0.7%) of sperm cells were disposed of. The introduced approach was further tested in a clinical setting by collecting and processing SF samples of PCa patients. The yield of isolated CTCs measured as high as 613 cells per SF sample in comparison with that of 6 cells from SF of healthy donors, holding significant promise for PCa diagnosis. The correlation analysis of the isolated CTC numbers with the standard prognostic parameters such as Gleason score and PSA serum level showed correlation coefficient values at 0.40 and 0.73, respectively. Taken together, our results show promise in the developed liquid biopsy technique to augment the existing diagnosis and prognosis of PCa.

Функциональная активность лейкоцитов в семенной жидкости при патоспермии

Assessment of leukocyte activity as the main source of ROS in seminal fluid of patients with normospermia and pathospermia using an original protocol based on the kinetic chemiluminescence method and adapted for semen analysis. A prospective study was attended by 95 men of reproductive age who applied to the Research Center of Medical Genetics (Moscow) for semen analysis. The material for the study were samples of native ejaculate. Chemiluminescent measurements were performed on a Lum-1200 chemiluminometer (DISoft, Russia) using the original method. Both in amplitude of basal and stimulated response, between the "normozoospermia", "pathozoospermia" and "pathozoospermia + leucospermia" groups, significant differences were obtained in the level of ROS production by leukocytes (p<0.05): the median level of basal chemiluminescence, normalized on the count of leukocytes was 0.13, 0.71 and 1.78, respectively; the median level of stimulated chemiluminescence normalized to the number of leukocytes was 0.62, 2.14, 5.94, respectively. The level of stimulated response did not exceed 0.5 arb. units in normozoospermic samples. In pathospermic groups, the level of stimulated response was low in about a third of semen samples, it was moderate in one third of patients, and high in one third of patients. A protocol previously developed for blood analysis was adapted to analyze the total ROS level produced by leukocytes in seminal fluid. In the groups of "pathozoospermia", "pathozoospermia + leucospermia", the level of basal ROS production by leukocytes was about 5 and 15 times higher than in the "normozoospermia" group, the level of stimulated ROS production was 3.5 and 9.5 times; this indicates oxidative stress, including with a normal number of leukocytes.

Reproductive Effects of some trace elements On Male Infertility In Thi-Qar Governorate/Iraq

&nbsp;&nbsp;&nbsp; The present study was carried out on 50 infertile males whose age range between (19-49) year and 16 male, who were apparently healthy, as a control group whose age range between (18-49) year. &nbsp;&nbsp;&nbsp; The studied males were classified into two groups according to their case health to infertile male and fertile male. &nbsp;&nbsp;&nbsp; The parameters measured were seminal fluid levels lead, cadmium, zinc, copper, chromium, magnesium, iron and cobalt. These parameters were estimated in seminal fluid of patients and control group. &nbsp;&nbsp;&nbsp; The results shown a highly significant different in levels of lead element(7.541a, 0.056b ), cadmium element(0.023a, 0.007b), zinc element(1.275a, 0.054b ), chromium element(0.408a, 0.090b), iron&nbsp; &nbsp; &nbsp; &nbsp;element(2.172a, 0.148b), cobalt element(0.022a, 0.008b), in primary infertile males when compared with in secondary infertile males(a: primary, b:secondry).The results shown a highly significant different in levels of lead element, cadmium element, zinc element, chromium element, iron element, cobalt element, in infertile men when compared with fertile men. &nbsp;&nbsp; The results illustrated highest level of concentrations elements in measurements was lead element levels and cadmium levels in infertile males when compared with control group. &nbsp;&nbsp;&nbsp; As well as lower levels of concentration metals was cobalt element levels in infertile males when compared with control group.&nbsp;&nbsp;&nbsp;&nbsp

P109 - Positive prostate 68GaPSMA-PET/CT correlates with detection of CD45-/PSMA+ non-sperm epithelial cells obtained by liquid biopsy of seminal fluid in patients with prostate cancer (PCa)

P109 - Positive prostate 68GaPSMA-PET/CT correlates with detection of CD45-/PSMA+ non-sperm epithelial cells obtained by liquid biopsy of seminal fluid in patients with prostate cancer (PCa)

The impact of obesity on seminal fluid in patients with male infertility

Data on the effect of obesity on seminal fluid and men fertility are inconsistent. The aim of this study was to evaluate the impact of body mass index (BMI) on semen characteristics in infertile men.A cross-sectional study was conducted on seventy-four infertile men who met inclusion criteria.Semen sample were collected and sperm concentration,progressive motility,total motility and normal sperm morphology were assessed in accordance with WHO 2010 criteria. For each patient weight and height were measure and patients were divided by BMI into normal weight (BMI: 18.5–24.9 kg/m2,n=30),overweight (BMI: 25–29.9 kg/m2,n=30) and obese (BMI:≥30 kg/m2, n=14). Seminal fluid parameters were compared among the three groups.Sperm concentration was lower in obese men but it did not differ significantly from those of normal weight and overweight infertile men (25.71±22.16,34.33±31.11,36.07±31.24 and million/ml respectively,P&gt;0.05). Sperm progressive motility,total motility and normal sperm morphology also were not significantly different among normal weight,overweight and obese infertile men.Our findings suggest that BMI may have no influence on sperm concentration,motility and normal morphology in infertile men.

The role of the level of biochemical markers of inflammation and protective systems of the organism in blood serum and in seminal fluid in patients with chronic prostatitis

Chronic prostatitis is one of the most common urological diseases. The most socially active groups of the population suffer the most from chronic prostatitis. Most complications of this disease lead to the most undesirable consequences: a decrease in potency, a pain syndrome that complicates the daily activities of patients, dysuria and the possibility of exacerbation of the inflammatory process, which manifests itself in a sharp increase in the exudative reaction, even before the abscess. The article discusses the results of observations of patients with chronic prostatitis in healthy men aged 20-45 years without somatic diseases. Studies were conducted to determine the role of the level of biochemical markers of inflammation of the body's defense systems in blood serum and in semen in patients with chronic prostatitis.

Роль уровня биохимических маркеров воспалительных и защитных систем организма в сыворотке крови и в семенной жидкости у больных с хроническим простатитом = The role of the level of biochemical markers of inflammation and protective systems of the organism in blood serum and in seminal fluid in patients with chronic prostatitis

Shuhtin V. V., Sknar V. N., Shuhtina I. N., Zukow W. Роль некоторых биохимических показателей у больных хроническим простатитом = The role of some biochemical parameters in patients with chronic prostatitis. Journal of Education, Health and Sport. 2016;6(2):11-17. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.45485 http://ojs.ukw.edu.pl/index.php/johs/article/view/3373 https://pbn.nauka.gov.pl/works/711693 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 01.01.2016. Revised 12.01.2016. Accepted: 31.01.2016. УДК 616.699:616.697-07:616.69-008.8-097 РОЛЬ НЕКОТОРЫХ БИОХИМИЧЕСКИХ ПОКАЗАТЕЛЕЙ У БОЛЬНЫХ ХРОНИЧЕСКИМ ПРОСТАТИТОМ THE ROLE OF SOME BIOCHEMICAL PARAMETERS IN PATIENTS WITH CHRONIC PROSTATITIS В. В. Шухтин 1 , В. Н. Скнарь 2 , І. М. Шухтіна 2 , В. А. Жуков 3 V. V. Shuhtin 1 , V. N. Sknar 2 , I. N. Shuhtina 2 , W. Zukow 3 1 ГП «Украинский научно-исследовательсий институт медицины транспорта» 2 Одесский национальный медицинский университет 3 Uniwersytet Kazimierza Wielkiego, Bydgoszcz 1 SE Ukrainian Scientific Research Institute of Transport Medicine, Ministry of Health of Ukraine, Odessa 2 Odessa National Medical University, Odessa 3 Kazimierz Wielki University, Bydgoszcz Abstract Chronic prostatitis, inflammatory disease of infectious origin with the possible accession of autoimmune and dysbiotic violations occur in almost 50% of men of working age. The aim of the study was to determine the level of biochemical markers of inflammation and protective systems in the two biological fluids - blood serum and in seminal fluid of patients with chronic prostatitis. Studies have been conducted in 15 patients with chronic prostatitis and 12 healthy individuals (males aged 20-45 years with no systemic diseases). The results show a decrease in somatic nonspecific immunity and the level of physiological antioxidant system, as well as the leading role of non-infectious factor in the development of chronic prostatitis. Determination of biochemical markers of the above will contribute to the improvement of the efficiency of diagnostic and pathogenetic therapy of this disease. Keywords: chronic prostatitis, biochemical markers, diagnosis. Реферат Хронический простатит, воспалительное заболевание инфекционного генеза с возможным присоединением аутоиммунных и дисбиотических нарушений, встречается почти у 50% мужчин трудоспособного возраста. Целью исследования явилось определение уровня биохимических маркеров воспаления и защитных систем организма в двух биологических средах - сыворотке крови и в семенной жидкости у больных хроническим простатитом. Исследования были проведены у 15 больных хроническим простатитом и у 12 здоровых лиц (мужчины в возрасте 20-45 лет без соматических заболеваний). Результаты свидетельствуют о снижении общесоматического неспецифического иммунитета и уровня физиологической антиоксидантной системы, а также о ведущей роли неинфекционного фактора в развитии хронического простатита. Определение показателей вышеуказанных биохимических маркеров будет способствовать усовершенствованию алгоритма диагностики и эффективности патогенетической терапии данной патологии. Ключевые слова: хронический простатит, биохимические маркеры, диагностика.

Роль уровня биохимических маркеров воспалительных и защитных систем организма в сыворотке крови и в семенной жидкости у больных с хроническим простатитом = The role of the level of biochemical markers of inflammation and protective systems of the organism in blood serum and in seminal fluid in patients with chronic prostatitis

Роль уровня биохимических маркеров воспалительных и защитных систем организма в сыворотке крови и в семенной жидкости у больных с хроническим простатитом = The role of the level of biochemical markers of inflammation and protective systems of the organism in blood serum and in seminal fluid in patients with chronic prostatitis

The role of the level of biochemical markers of inflammation and protective systems of the organism in blood serum and in seminal fluid in patients with chronic prostatitis

The role of the level of biochemical markers of inflammation and protective systems of the organism in blood serum and in seminal fluid in patients with chronic prostatitis

Procoagulant tissue factor-exposing vesicles in human seminal fluid

Recent studies indicate that various types of vesicles, like microparticles (MP) and exosomes, are present in blood, saliva, bone marrow, urine and synovial fluid. These vesicles, which are released upon activation or shear stress, are thought to play a role in coagulation, neovascularisation, inflammation and intercellular signalling. Seminal fluid is a cell-, sperm- and protein-rich suspension. Although seminal fluid is known to contain vesicles like prostasomes, MP and exosomes have never been characterised. Therefore, the aim of our study was to analyse and characterise vesicles in seminal fluid in male partners of patients undergoing controlled ovarian stimulation for IVF/ICSI. MP from seminal fluid of patients during routine IVF/ICSI procedures were detected and analysed with flow cytometry (FACS) and transmission electron microscopy (TEM), using antibodies against tissue factor (TF), CD10, CD13, CD26 and annexin V. The coagulant properties of vesicles were studied using a fibrin generation test. MP were detected in human seminal fluid by both flow cytometry and TEM. Seminal fluid-derived MP expressed CD10, CD13, CD26 and TF, which was highly procoagulant and a powerful trigger of the extrinsic pathway of coagulation. The extent to which the procoagulant activity of MP in seminal fluid contributes to the implantation process itself and therefore affects human reproduction needs to be further elucidated.

Are Metal Ions in the Seminal Fluid of Patients After Metal-on-Metal Total Hip Arthroplasty a Concern?

Are Metal Ions in the Seminal Fluid of Patients After Metal-on-Metal Total Hip Arthroplasty a Concern?

Imbalance in Seminal Fluid MIF Indicates Male Infertility

Macrophage migration inhibitory factor (MIF) is a ubiquitous cytokine that functions in reproduction and plays an important role in sperm maturation and motility. Here we reveal a correlation between MIF levels in human seminal fluid and fertility status. We identify an abnormal biphasic profile of MIF in the seminal fluid of patients with impaired sperm parameters. Our findings may be of interest for the development of a diagnostic method for fertility status.

Imbalance in seminal fluid MIF indicates male infertility.

Macrophage migration inhibitory factor (MIF) is a ubiquitous cytokine that functions in reproduction and plays an important role in sperm maturation and motility. Here we reveal a correlation between MIF levels in human seminal fluid and fertility status. We identify an abnormal biphasic profile of MIF in the seminal fluid of patients with impaired sperm parameters. Our findings may be of interest for the development of a diagnostic method for fertility status.

Altered sperm function or sperm antibodies are not associated with chlamydial antibodies in infertile men with leucocytospermia

Leucocytospermia, defined as a concentration of more than 10(6) leucocytes/ml of seminal fluid in patients without clinical symptoms due to an adnexitis, is seen in about 10% of patients in an infertility department. Infection with Chlamydia trachomatis is possibly relevant as other pathogenic bacteria were not cultured from the semen in significant numbers. University Clinic, Department of Andrology. Two hundred and seven patients attending the department for male infertility investigation. Analysis on each semen sample included determination of leucocyte count and the MAR test for the detection of sperm antibodies. Chlamydial antibodies in semen were determined using an on-slide enzyme immunoassay. No differences between leucocyte counts in patients with and without chlamydial antibodies were detected. In addition, no differences in the sperm parameters or results of MAR-tests in these two groups was seen. There were no correlations between the leucocyte count and sperm parameters, including the MAR-test results. We conclude that antibodies to chlamydiae in semen are not associated with leucocytospermia. Leucocytospermia per se does not appear to be significant for the sperm functions and immune responses to sperm.

Altered sperm function or sperm antibodies are not associated with chlamydial antibodies in infertile men with leucocytospermia

Objective Leucocytospermia, defined as a concentration of more than 106 leucocytes/ml of seminal fluid in patients without clinical symptoms due to an adnexitis, is seen in about 10% of patients in an infertility department. Infection with Chlamydia trachomatis is possibly relevant as other pathogenic bacteria were not cultured from the semen in significant numbers.Setting University Clinic, Department of Andrology.Patients Two hundred and seven patients attending the department for male infertility investigation.Methods Analysis on each semen sample included determination of leucocyte count and the MAR test for the detection of sperm antibodies. Chlamydial antibodies in semen were determined using an on-slide enzyme immunoassay.Results No differences between leucocyte counts in patients with and without chlamydial antibodies were detected. In addition, no differences in the sperm parameters or results of MAR-tests in these two groups was seen. There were no correlations between the leucocyte count and sperm parameters, including the MAR-test results.Conclusions We conclude that antibodies to chlamydiae in semen are not associated with leucocytospermia. Leucocytospermia per se does not appear to be significant for the sperm functions and immune responses to sperm.

Detection of HIV-Related Protein in Testes and Prostates of Patients with AIDS

The testes and prostates of 14 patients with acquired immune deficiency syndrome (AIDS) for whom autopsies were performed were examined for the presence of human immunodeficiency virus (HIV) and the pathologic alterations seen in AIDS. Histologically, the testes contained peritubular fibrosis and variable spermatogenic arrest, which were inconsistent with the young age of these patients. There were also numerous foci of germ cell degeneration and occasional germ cell loss. The Leydig cells were atrophic and decreased in number. The prostates contained increased numbers of concretions. Sections of testis and prostate were stained with an anti-HIV P17 monoclonal antibody with the use of the avidin-biotin technique. Small scattered foci of positive staining were identified in 8 of 14 testes (57%). They were located over one or several degenerating germ cells and the surrounding Sertoli cells. In addition, in 9 of 14 prostates (64%) there were a few minute foci of positive staining in several adjacent glandular epithelial cells. In one case the testis was positive and the prostate was negative, whereas in two cases the testes were negative and the prostate positive. In contrast, 22 testes and 22 prostates of control non-AIDS patients, read double-blind, were negative. The positive controls were HIV-infected tissue culture HUT 78 lymphoma cells in which there were many scattered positive cells. The results indicate the presence of focal HIV-associated protein in the testes and prostates of patients with AIDS, particularly within the foci of germ cell degeneration. The present observations are in accordance with previous research demonstrating the presence of HIV in the seminal fluid of patients with AIDS and appears to indicate the presence of an infection of the male genital tract by the HIV virus.

Carcinoma in situ of the testis: aneuploid cells in semen.

The content of cellular DNA in ejaculates from eight patients with carcinoma in situ of the testis and 26 controls without evidence of testicular neoplasia was studied by flow cytometry. An aneuploid cell population with a ploidy value similar to that found for carcinoma in situ cells was detected in seminal fluid from four of the eight men with carcinoma in situ but in none of the controls. One year after orchidectomy or local irradiation in these four men no aneuploid cells were found in the semen. These findings show that a detectable proportion of malignant germ cells may be released into the seminal fluid of patients with carcinoma in situ of the testis. Analysis of seminal fluid may therefore aid in screening for early neoplasia of the testis.

Detection of Testicular Neoplasms by Cytological Examination of Seminal Fluid

The technique for detection of neoplastic cells in the seminal fluid of patients with testicular malignancies is presented. This preliminary study of 7 patients with testicular tumors and 50 controls demonstrates the possibility of using cytological examination of the sperm as the screening method for testicular tumors.

Reduced activity of Mg2+- and Ca2+-dependent adenosine triphosphatase in seminal fluid of patients with oligozoospermia.

Mg2+- and Ca2+-dependent ATPase activity, fructose and divalent cation concentrations were determined in seminal plasmas from men with oligozoospermic ejaculates and from those with normal sperm count. The mean activity of the Mg2+- and Ca2+-stimulated ATPase from the former was 59 nmol . 0.1 ml-1 . This is significantly (p less than 0.005) lower than for men with a normal sperm count (corresponding figure is 87 nmol . 0.1m1-1 . min-1). As regards the concentrations of Mg2+, Ca2+ and Zn2+ in the seminal plasmas from these two groups there was no significant difference. The same was also valid for the fructose concentrations. The Mg2+- and Ca2+-stimulated ATPase activity as well as fructose and divalent cation concentrations were also determined in seminal plasmas from men before and after vasectomy. After vasectomy, only the ejaculates devoid of spermatozoa were included in the comparative studies. No significant difference was observed between the seminal plasmas from men before and after vasectomy. These findings support the view that the Mg2+- and Ca2+-stimulated ATPase system does not derive from the spermatozoa. Possible explanations for the significantly lowered Mg2+- and Ca2+-dependent ATPase activity from patients with oligozoospermia are given.

Diagnostic significance of levels of immunoglobulin a in seminal fluid of patients with prostatic disease

Immunoglobulin A (IgA) levels in semen and in prostatic fluid of 48 patients with benign and malignant diseases of the prostate were estimated. It is speculated from this small study that the IgA levels rise in benign prostatic hyperplasia; in early adenocarcinoma of the prostate (Stages A and B) the rise is furthered, while in the late stages (Stages C and D) the levels fall.