Semimembranosus Research Articles

Dystrophic Calcified Mass Found in the Semimembranosus Muscle in a Cadaver: A Case Report

Most of the soft tissue calcified mass formations are sporadic incidences that occur in an idiopathic form. Ectopic calcification changes were seen in various soft tissues beyond the skeleton. They are often asymptomatic masses located in the superficial or deeper aspect of the body, but may not be related to the bone directly. Increased levels of serum calcium, phosphate, or Vitamin D hypervitaminosis may be associated with the calcification process, causing mass accretion in the tissues like bones of limbs, and cranium, or showing some dental abnormalities. During the routine dissection of the hamstring compartment of the left lower limb in a male cadaver aged around 70 years in the Department of Anatomy, we noticed nodular fragments and lengthy slender pieces, which were hard in consistency and bony in its appearance found within the semimembranosus muscle. The histopathology of these masses was revealed as “Dystrophic Calcified Masses”. A holistic description of calcification changes in the soft tissues will be considered in the article. Keywords: calcification, dystrophy, hypervitaminosis

The architectural characteristics of the hamstring muscles do not differ between male and female elite-level rugby union players.

Purpose: To determine whether differences exist in the architectural characteristics of the hamstring muscles of elite-level male and female rugby union players. Methods: Forty elite-level rugby union players (male n = 20, female n = 20) participated in this cross-sectional study. A sonographer acquired static ultrasound images using a 92mm linear transducer to quantify (via a semi-automated tracing software tool) the architectural characteristics (muscle length, fascicle length, pennation angle, and muscle thickness) of the biceps femoris long head and semimembranosus muscles of participants' left limb. Muscle length and muscle thickness of the biceps femoris short head and semitendinosus muscles of participants' left limb were also quantified. Bonferroni adjusted independent samples t-tests were performed to evaluate whether differences exist in the architectural characteristics of the hamstring muscles of elite-level male and female rugby union players. Results: There were no significant differences in fascicle length or pennation angle of the hamstring muscles of elite-level male and female rugby union players. Some significant differences in muscle thickness (biceps femoris short head, and semimembranosus) and muscle length (biceps femoris short head, biceps femoris long head, semitendinosus, and semimembranosus) were observed; in all cases the male players had thicker and longer muscles. Conclusion: At a group level, hamstring muscle fascicle length and pennation angle are unlikely to be a sex-specific intrinsic risk factor for Hamstring strain injuries.

WYBRANE SKŁADNIKI MINERALNE MI ˛ESNI SZKIELETOWYCH DANIELI ( ´ DAMA DAMA) I JELENI (CERVUS ELAPHUS) Z EKOLOGICZNEGO SYSTEMU CHOWU

The aim of the present study was to identify and assess the determinants of the content of Ca, Mg, Zn, Fe, Cd, and Pb in longissimus lumborum (LL) and semimembranosus (SM) muscles of fallow deer and red deer reared in the organic system. The concentrations of 6 elements (Ca, Mg, Zn, Fe, Cd, Pb) in fallow deer and red deer meat produced in the organic system in Poland were analyzed with the use of inductively coupled plasma mass spectrometry. The animals, i.e. 12 ca. 2- and 3-year-old fallow deer (Dama dama) and 12 ca. 3- and 4-year-old red deer (Cervus elaphus), were culled during the autumn season. Both groups of animals comprised 6 males and 6 females. A significant effect of the species on the content of selected minerals in the meat was noted. It was found that the LL muscle from the fallow deer was a rich source of Ca, Mg, and Zn, and the meat from the red deer had high amounts of Zn and Fe. The age of the animals had a significant effect on the level of Mg in the red deer meat.

Anatomical classification of adductor magnus perforator flap and its application in head and neck reconstruction

Objective: To investigate the anatomical classification of adductor magnus perforator flap and its application in head and neck reconstruction. Methods: From January 2017 to January 2020, Hunan Cancer Hospital treated 27 cases of oral tumor patients (15 cases of tongue cancer, 9 cases of gingival cancer and 3 cases of buccal cancer), including 24 males and 3 females, aged 31-56 years old. The course of disease was 1-12 months. Secondary soft tissue defects with the sizes of 5.0 cm × 3.5 cm to 11.0 cm × 8.0 cm were left after radical resection of the tumors, and were repaired with free adductor magnus perforator flaps. The flaps based on the origing locations of perforator vessels were divided into three categories: ① intramuscular perforator: vessel originated between the gracilis muscle and the adductor magnus or passed through a few adductor magnus muscles; ② adductor magnus middle layer perforator: vessel run between the deep and superficial layers of adductor magnus; ③ adductor magnus deep layer perforator: vessel run between the deep layer of adductor magnus and the semimembranous muscle. Descriptive analysis was used in this research. Results: Perforator vessels of adductor magnus were found in all cases, with a total of 62 perforator branches of adductor magnus. The anatomical classification of the perforator vessels was as follows: 12 branches for class ①, 31 branches for class ② and 19 branches for class ③. The vascular pedicles of the free adductor major perforator flaps included type ① for 3 cases, type ② for 16 cases and type ③ for 8 cases. All 27 flaps survived and the donor sites were closed directly. In 18 cases, the perforator arteries and the venae comitan were respectively anastomosed with the superior thyroid arteries and veins. In 9 cases, the pedicle arteries and the venae comitan were respectively anastomosed with the facial arteries and veins. Follow up for 12-40 months showed that the appearances of the flaps and the swallowing and language functions of patients were satisfactory, apart from linear scars were left in the donor sites with no significant affect on the functions of thigh. Local recurrence occurred in 3 cases and radical surgeries were performed again followed by repairs with pedicled pectoralis major myocutaneous flaps. Cervical lymph node metastasis occurred in 2 cases and cervical lymph node dissection was performed again. Conclusions: The adductor magnus perforator flap has soft texture, constant perforator vessel anatomy, flexible donor location and harvesting forms, and less damage to the donor site. It is an ideal choice for postoperative reconstruction in head and neck tumors.

Supplementation of palmitoleic acid improved piglet growth and reduced body temperature drop upon cold exposure.

Piglet survival is a major challenge in the first few days postpartum and interventions during this period may improve survival and growth. This study investigated the effects of palmitoleic acid (C16:1n-7; PA) supplementation on growth performance, body temperature, fatty acid (FA), and energy metabolism in milk-replacer-fed piglets. Forty-eight piglets were stratified by body weight and randomly assigned to one of four dietary treatments (0%, 1%, 2%, and 3% PA supplementation as a percent of milk replacer) and given the diet through an orogastric tube. They were fed dietary treatments every 2 h for 4 d in the first week postpartum and all were sacrificed at the end of the experiment. The piglets were weighed daily, and half in each dietary treatment group, the same piglets each day, were exposed daily to a lower temperature for 2 h. Plasma samples were collected immediately before sacrifice for analyses of FA and other plasma metabolites. The weight of organs and empty body weight were determined after sacrifice. Liver and semimembranosus muscle tissue samples were collected and analyzed for FA content. Contents of C16:1n-7 and C18:1n-7 in both plasma and liver (P < 0.001), and C16:1n-7 in semimembranosus muscle (P < 0.001) increased linearly as PA supplementation increased. Most plasma FA levels (except C16:1n-7, C16:1n-9, and C22:5n-3) were lower in piglets exposed to lower temperatures than those that were not. Plasma glucose, triglycerides, and lactate dehydrogenase levels increased linearly with PA supplementation (P < 0.001). Piglets' average daily gain, liver glycogen pool, liver weight, and gallbladder weight increased linearly (P < 0.05, P < 0.01, P < 0.05, and P < 0.001, respectively), but lung weight, liver nitrogen content, and body temperature drop decreased linearly (P < 0.01, P < 0.001, and P < 0.05, respectively) with PA supplementation. Piglets exposed to low temperature had greater liver nitrogen (P < 0.05) and lactate dehydrogenase (P < 0.001) contents but had lower liver weight (P < 0.01) and plasma lactate concentration (P < 0.05) than those that were not. In conclusion, this study demonstrated the importance of PA on the growth performance of the piglets by increasing their average daily gain and decreasing a drop in body temperature upon cold exposure, most likely due to a modified energy metabolism.

Meat Quality in Rabbit (Oryctolagus cuniculus) and Hare (Lepus europaeus Pallas)—A Nutritional and Technological Perspective

This study aimed to nutritionally and technologically characterize the meat produced by rabbit (Oryctolagus cuniculus, Flemish Giant breed, 50 farmed individuals) and hare (Lepus europaeus Pallas, 50 hunted individuals). Muscles were sampled from several carcass regions: dorsal torso—Longissimus dorsi (LD), thigh—Semimembranosus (SM), and upper arm—Triceps brachii (TB). To better depict the meat’s nutritional quality, the proximate composition and fatty acid profile were assessed, and then gross energy content and lipid sanogenic indices (Polyunsaturation—PI, atherogenic—AI, thrombogenic—TI, hypocholersyerolemic/hypercholesterolemic ratio—h/H, Nutritional Value Index—NVI) were calculated. pH values at 24 and 48 h post-slaughter, cooking loss (CL), and water-holding capacity (WHC) were the investigated technological quality traits. Gross energy was higher in rabbit TB samples, compared with hare, due to more accumulated lipids (p &lt; 0.001). pH value was higher for TB muscles in both species; the WHC was higher for hare (p &lt; 0.001), and CL was higher for rabbit (p &lt; 0.001). The PI values were 6.72 in hare and 4.59 in rabbit, AI reached 0.78 in hare and 0.73 in rabbit, TI was calculated at 0.66 in hare and 0.39 in rabbit, and the h/H ratio reached 3.57 in hare and 1.97 in rabbit, while the NVI was 1.48 in hare and 1.34 in rabbit samples. Meat from both species is nutritionally valuable for human consumers, meeting nutritional values better than the meat of farmed or other wild species of fowl and mammals. Hare meat was found to be healthier than rabbit in terms of lower fat content, lighter energy, and better lipid health indices.

Correlations Between Hamstring Muscle Architecture, Maturation, and Anthropometric Measures in Academy Soccer Players.

Muscle architecture is associated with motor performance and muscle injury. While muscle architecture and knee-flexor eccentric strength change with growth, the influence of anthropometric measures on these properties is rarely considered. This study aimed to investigate the relationship between hamstring muscle architecture and knee-flexor eccentric strength with anthropometric measurements. Sixty male footballers (16.6 [1.05]y) from the U16, U17, and U19 teams of an elite soccer club were included in this study. Fascicle length, pennation angle, and muscle thickness of the biceps femoris long head (BFlh) and semimembranosus muscles were measured in both legs using ultrasound. Knee-flexor eccentric strength, height, body mass, leg length, femur length, and peak height velocity (PHV) were measured within 1 week of the ultrasound images. A stepwise regression and 1-way analysis of variance tests were used to evaluate the effects of age, maturity, and anthropometric measurements on muscle properties. Variance within BFlh and semimembranosus muscle thickness (r < .61), semimembranosus pennation angle (r < .58), and knee-flexor eccentric strength (r = .50) were highly related to body mass. We observed no significant correlations between muscle architecture and age (P > .29). However, moderately greater BFlh muscle thickness was shown for the post-PHV compared with the PHV group (effect size ± 90% CI: 0.72 ± 0.49). In conclusion, weak correlations between muscle architecture and anthropometric measurements suggest that other factors (ie,genetics, training regimen) influence muscle architecture. The moderate effect of maturity on BFlh muscle thickness strongly suggests post-PHV hypertrophy of the BFlh muscle. Our results confirmed previous findings that eccentric knee-flexor strength is influenced by body mass.

Blood hormone concentrations as markers and predictors of porcine meat production and quality?

Context The applicability of hormones in the prediction of meat production in non-ruminant species has been poorly examined. Aims To examine the applicability of plasma concentrations of leptin, estradiol, progesterone and insulin-like growth-factor I (IGF-I) for characterisation and early prediction of the production and quality of meat in gilts. Methods Animals were compared at 150 days of age with below (‘low’) and above (‘high’) median hormone concentrations and their traits of meat production and quality at the age of 200 days. Key results Animals with a high leptin concentration in blood at the age of 150 days had higher slaughter and carcass weights and weight of ham, but a lower lean meat content at the age of 200 days, than did the gilts with a low leptin concentration. Animals with a high estradiol concentration had a higher carcass weight, weight of ham and backfat thickness and a lower electrical conductivity of musculus semimembranosus than did animals with a low estradiol concentration. High blood IGF-I concentration was associated with a higher slaughter weight, carcass weight and lean meat content. No significant differences between the animals with ‘high’ and ‘low’ progesterone concentration and meat characteristics have been found. Conclusions These observations confirm the involvement of leptin, IGF-I and estradiol in up-regulation of meat production in pigs. Furthermore, they suggest that leptin can be useful for characterisation and prediction of fat production, whereas IGF-I and estradiol (but not progesterone) could be both markers and predictors of porcine growth and meat production. Implications The blood concentrations of these hormones could help in characterisation and selection of pigs for meat production.

Leg cuts from Awaasi lambs fed a diet with varying levels of Rhus coriaria L., Physical dissection and chemical composition.

This research was conducted in a private slaughterhouse in Samarra from 1/5/2019 to 1/8/2019. Sixteen Awassi lambs were raised with an average body weight (of 20±25) kg and an age of about (5.5 to 6) months. We divided the lambs into four treatments. The first treatment was without additives, called control; the second treatment was 1% Sumac, the third treatment was 3% Sumac, and the last one was the fourth treatment with 5% Sumac. The results showed significant differences between treatments in biceps femoris and semimembranosus muscle (treatment 3 the female) were (180.9±10.66)g, ( 168.4±5.72)g respectively. And a highly significant difference in adductor muscle (treatment 3, the female) was (75.6±2.58)g compared with the control (62.85±5.93)g. significant differences in treatment 3 in Semitendinosus muscle between males and females were( 57.2±3.65) and (74.4±3.02)g, respectively. The rectus femurs showed significant differences between treatments 3 and 4 in females were( 99.6±8.76) and (76.3±6.98)g, respectively. In physical dissection, a low significant difference in treatment 4 in lean was (56.3+3.66)%. The lean percentage in treatment 3 of the female was (59.2±0.99) %. About chemical analysis, the high percentage of moisture in the male in treatments 2,3 and 4 were (72.7±0.14),( 72.8±0.21),(and 72.8±0.21)%, respectively. Keywords: leg cut, physical dissection, Rhus Coriaria L.

Fatigue-induced changes in hamstrings’ active muscle stiffness: effect of contraction type and implications for strain injuries

PurposeHamstring strain injuries may occur due to differential fatigue and compromised mechanical properties among the hamstring muscles. We examined (1) the effect of fatigue on hamstrings active muscle stiffness, and (2) whether contraction type affects active muscle stiffness changes during a submaximal fatiguing task.MethodsNine healthy males completed 99 submaximal knee flexions in isometric (ISO), concentric (CON), and eccentric (ECC) conditions. We measured the knee flexor maximal voluntary torque (MVT) (pre/post), shear wave velocity (SWV) during contraction and transverse relaxation times (T2) (pre/post) in biceps femoris long head (BFlh), semitendinosus (ST), and semimembranosus (SM) muscles.ResultsMVT decreased substantially after all conditions (− 18.4 to − 33.6%). The average relative torque sustained during the task was lower in CON than ISO and ECC, but absolute torque was similar. SWV interindividual responses were highly variable across muscles and contraction types. On average, BFlh SWV tended to increase in ISO (0.4 m/s, 4.5%, p = 0.064) but decreased in ECC condition (− 0.8 m/s, − 7.7%, p < 0.01). ST SWV decreased in CON (− 1.1 m/s, − 9.0%, p < 0.01), while it remained unchanged in ISO and ECC. SM SWV decreased in CON (− 0.8 m/s, − 8.1%, p < 0.01), but it was unaffected in ISO and variable in ECC.ConclusionFatigue has a differential effect on the mechanical properties of the constituent hamstring muscles, as measured with shear wave elastography, depending upon contraction type. We found preliminary evidence that BFlh is more fatigued than ST or SM during eccentric contractions, which may explain its susceptibility to strain injuries.

Effect of intramuscular fat in the thigh muscles on muscle architecture and physical performance in the middle-aged women with knee osteoarthritis

BackgroundWe investigated intramuscular fat (IMF) in quadriceps femoris (QF) and hamstring muscles in the middle-aged women with knee osteoarthritis (KOA). We also examined the relationship between muscular infiltration of QF and hamstring muscles and muscle architecture and physical performance of the women with KOA. MethodsIn this cross-sectional study, 72 women were included. Body muscle and fat mass were measured by BIA, isometric muscle strength was evaluated by hand-held dynamometer. IMF and muscle architecture were calculated from rectus femoris (RF), vastus intermedius (VIM), vastus lateralis (VL), vastus medialis (VM), biceps femoris (BF), semitendinosus (ST) and semimembranosus (SM) using B-mode ultrasonography. KOA-related symptoms and functions were assessed with KOOS. The functional performance assessments were evaluated with Stair Climbing Test, 20-Meter Walking Test. ResultsWomen with KOA had more IMF in RF, VIM, VL, VM and BF, ST, SM muscles compared to the healthy women. Pennation angles decreased as the IMF in the RF, VM, BF and ST decreased. As the IMF of the RF and VM increased isometric knee extensor strength decreased and KOOS symptom score, pain score and ADL score increased in women with KOA. Walking and stair climbing speed deteriorated as the IMF in RF, VIM, VM, BF increased in the middle-aged women. As the IMF in BF increased isometric knee flexor strength decreased and KOOS scores increased. Physical performance scores deteriorated as the IMF in BF increased in middle-aged women with KOA. ConclusionIMF in QF and hamstring muscles were higher in the middle-aged women with KOA group compared with that in the healthy group. Weakness of the QF and hamstring muscles may due to the changes in architectural properties of muscle depending on muscular infiltration. IMF in knee muscles is an important determining factor in performance and physical function of middle-aged women with KOA.

Role of Intramuscular Connective Tissue in Water Holding Capacity of Porcine Muscles

This study evaluated the influence of intramuscular connective tissue (IMCT) on structural shrinkage and water loss during cooking. Longissimus thoracis (LT), semimembranosus (SM) and semitendinosus (ST) muscles were cut and boiled for 30 min in boiling water, followed by detection of water holding capacity (WHC), tenderness, fiber volume shrinkage and protein denaturation. Compared with LT and SM, ST had the best WHC and lowest WBSF and area shrinkage ratio. The mobility of immobilized water (T22) was key to holding the water of meat. ST contained the highest content of total and heat-soluble collagen. On the contrary, ST showed the lowest content of cross-links and decorin, which indicate the IMCT strength of ST is weaker than the other two. The heat-soluble collagen is positively correlated to T22. The shrinkage of heat-insoluble IMCT on WHC and WBSF may partly depend on the structural strength changes of IMCT components rather than solely caused by quantitative changes of IMCT.

Fatty acid and conjugated linoleic acid content of Anatolian buffaloes at different muscle types and slaughter weight.

This research was performed to detect tissue fatty acid (FA) composition and conjugated linoleic acid (CLA) content in Anatolian buffaloes at different muscle types (MTs) and slaughter weights (SWs). The research was carried out on a private commercial livestock farm in Tokat. The research's animal material comprised 20 Anatolian buffalo calves with approximately 100kg body weights, weaned at about 150days of age.Before the experiment started, the calves were randomly dividedinto two slaughter groups (SW) as low weight (LW=225 kg) and high weight (HW=325 kg). Ten calves from each of the two experimental groups, which were intensively fed, were slaughtered in two different weights. After the slaughtering, FA composition and CLA content of the Semimembranosus (SM), Semitendinosus (ST), and Triceps brachii (TB) muscle tissues of the animals were examined. The study determined that palmitoleic acid (C16:1) and stearic acid (C18:0) were affected by MTs and oleic acid (C18:1) and α-linolenic acid (C18:3 n-3) were affected by SW (P < 0.05). The highest CLA was in the ST muscle type (0.298) and the LW group (0.289) of the SW groups (P > 0.05). With the increase of SW (in LW and HW groups), n-6/n3 (SM: 7.783 and 6.533; ST: 8.115 and 7.859; TB: 8.416 and 8.215) (P > 0.05) and PUFA ratio decreased (P < 0.05). The SW increase raised the SFA ratio in the SM muscle (P < 0.05) while lowering it in the TB muscle (P > 0.05). Again, with the increase in SW, AI and TI values increased in SM and ST muscles, while the same index values decreased in TB muscle (P > 0.05). In conclusion,when considering the PUFA/SFA ratio and the beneficial effects of CLAs on human health, ST in the MT and LW groups in SW, and thus ST and LW in MT and SW were prominent in Anatolian buffaloes.

The Quality of Salted Sun-Dried Meat from Young Nellore Bulls Fed Diets with Lauric Acid.

This study aims to evaluate the quality of salted sun-dried meat from young bulls (Nellore cattle) fed with a diet containing 0.0, 0.5, 1.0 and 1.5% of lauric acid in the total dry matter (DM). Thirty-two Nellore bulls with initial body weight of 368 ± 32 kg were used. A linear decrease (p < 0.05) in pH and protein content of the salted sun-dried meat was observed with the inclusion of lauric acid. The moisture, ash, lipid, collagen content, water-holding capacity, cooking loss, color indexes (L*, a*, b*, C*), and shear force were not affected. Lipid oxidation at 7 days of storage increased linearly in the salted sun-dried meat. Most of the fatty acid composition of the salted sun-dried meat from the semimembranosus muscle of young bulls was not influenced (p > 0.05) by the lauric acid inclusion in the bulls’ diet. However, there was a linear increase (p < 0.05) in the SFA lauric acid (C12:0), PUFAn-3 EPA (C20:5n − 3) and DHA (C22:6n − 3), and a quadratic increase in the PUFAn-6 arachidonic (C20:4n − 6) due to lauric acid addition from palm kernel oil in the diet. There was a liner increase (p < 0.05) in the total ∑PUFA, ∑n − 6, ∑n − 3 contents of salted sun-dried meat from the semimembranosus muscle of young bulls and the h:H health index of the level of lauric acid inclusion in bull’s diet. In contrast, the thrombogenicity health index (TI) and ∑n − 6:∑n − 3 ratio content in salted sun-dried meat from the semimembranosus muscle of young bulls presented a linear decrease (p < 0.05) due to lauric acid addition in the bulls’ diet. Lauric acid (C12:0) inclusion up to 1.5% in the diet of young Nellore bull improved the fatty acid composition of the salted sun-dried meat, increasing EPA, DHA, n − 6 and n − 3, TI, and h:H indexes, which are associated with a better lipid quality of meat products, and further improves tenderness at the highest concentration.

Aging-related changes in knee flexor muscle strength and cross-sectional area.

Weakening muscle strength around the knee tends to render it vulnerable to aging-related damage. This study aimed to examine the association between knee flexor muscle strength and its cross-sectional area (CSA). We also evaluated aging-related changes in flexor muscle strength and CSA. We retrospectively analyzed 252 patients with acute-onset knee pain (<1 month) between September 2006 and August 2009 in accordance with the Strengthening the Reporting of Observational studies in Epidemiology statement. The CSA of each knee flexor muscle (biceps femoris, sartorius, gracilis, semitendinosus (ST), and semimembranosus (SM)) was measured on magnetic resonance imaging axial images at the suprapatellar level. We evaluated flexor muscle strength (peak torque in N.m) using a Cybex dynamometer at 60°/second and 180°/second and its correlation with CSA. In total, 252 patients (mean age, 34.5 years; range, 11 to 66 years; 184 men and 68 women) were included in this study. No significant intergroup differences in demographic data such as sex or body mass index were found. Mean CSA was 605.4 mm2 for the SM, 444.7 mm2 for the biceps femoris, 282 mm2 for the sartorius, 55.4 mm2 for the ST, and 34.1 mm2 for the gracilis. Mean peak torques were 67.4 N.m and 52.7 N.m at 60°/second and 180°/second, respectively. CSA was positively correlated with flexion strengths of 60°/second (R = 0.363, P < .001) and 180°/second (R = 0.354, P < .001). Muscle strength was associated with CSA in all muscles but the gracilis (R = 0.056, P = .375). Flexion strength decreased significantly with aging from the thirties. Total CSA decreased with aging (r = −0.247, P < .001). The CSA of the biceps femoris, sartorius, SM, and ST decreased significantly, whereas that of the gracilis tended to decrease non-significantly with aging. Flexor muscle strength was associated with total muscle CSA on magnetic resonance imaging and the CSA of every muscle except the gracilis. Flexion strength decreased significantly with aging after the twenties, while total CSA decreased significantly with aging. The CSA of all flexor muscles decreased significantly with aging, whereas that of the gracilis decreased only slightly.

Comparison of Lipid Properties and Cadmium and Lead Content in Red Deer (Cervus elaphus) Meat from Three Feeding Grounds.

Simple SummaryAs a product originating from the most natural living conditions with free foraging, red deer (Cervus elaphus) meat is becoming increasingly popular among consumers. A greater supply of this type of meat can be ensured by dynamically developing red deer farming on permanent grasslands. The study presents a comparison of the properties of lipids and the content of cadmium and lead in the LL and SM muscles of red deer kept in forest, organic farm, and conventional farm feeding grounds. The greater species and phytoncide diversity in the organic feeding ground enriched the quantitative profile and significantly increased the content of beneficial polyunsaturated fatty acids (FAs) (CLA—conjugated linoleic acid, ALA—alpha-linolenic acid, AA—arachidonic acid, and EPA—eicosapentaenoic acid) and the sum of polyunsaturated fatty acids (PUFAs), n-6 PUFA, and n-3 PUFA, in comparison with conventional farm and forest feeding grounds. It also contributed to a significantly lower n-6/n-3 ratio in both muscles. The study provides valuable data on the nutritional value and ecological safety of venison, which should be regarded as a health-enhancing food product. Furthermore, the present results can help breeders to make decisions about the locations of farms and the choice of red deer nutrition strategies.The aim of the study was to compare the properties of lipids and the content of cadmium and lead in the meat of red deer (Cervus elaphus) kept in a forest feeding ground (FFG) and on organic and conventional farms in Poland. Longissimus lumborum (LL) and Musculus semimembranosus (SM) muscles were collected for the study from 36 red deer carcasses in an equal sex and age ratio (3 and 4 years old). From April to October, the animals consumed only the vegetation growing in the feeding grounds. The floristic and phytoncide composition, as well as the fodder value, of the feeding grounds were assessed. Additionally, the intramuscular fat and cholesterol content, the profile, sum, and ratios of fatty acids (FAs), lipid nutraceutical parameters, and cadmium and lead content were determined in LL and SM. The plant composition comprised 116 species in the organic feeding ground (OFG) and 78 species in the conventional feeding ground (CFG). The LL and SM muscles of the red deer from the organic and forest feeding grounds exhibited significantly lower amounts of fat and cholesterol than those from the conventional system. The differences in the fatty acid composition between the three groups were quite small. Differences in intramuscular fat content contributed to a higher percentage of PUFAs in the FFG and OFG, in comparison to the CFG. In all types of feeding ground, the cadmium (0.002 to 0.008 mg/kg) and lead (0.009 to 0.019 mg/kg) content determined in the meat did not pose a threat to consumers.

Marinating and Grilling as Methods of Sensory Enhancement of Sous Vide Beef from Holstein-Friesian Bulls

An attempt was made to identify technological solutions that would improve the quality of products from the meat of Holstein-Friesian (HF) bulls, with particular emphasis on standardising the quality of the longissimus lumborum (LL) and semimembranosus (SM) muscles. Marinating (Mar) and grilling (Grill) were used in combination with the sous vide (SV) method. The effects of the type of muscle (LL or SM) and the applied treatments (SV, SVMar, and SVGrill) on the yield, colour, maximum shear force (WBSF), tenderness, juiciness, and aroma intensity of grilled and marinated meat were determined. The applied treatments had a significant influence on the meat yield (p &lt; 0.001), WBSF (p &lt; 0.001), tenderness (p &lt; 0.01), and juiciness (p &lt; 0.05) assessed in sensory evaluation. Additional treatments supported the optimisation of the aroma and taste of LL and SM and reduced the WBSF. The SVMar method optimised the beef’s tenderness, and therefore, it can be recommended for preparing meat from HF bulls for consumption.

Relación entre el crecimiento muscular y la expresión de miosina en cerdos en diferentes etapas de crecimiento

Nineteen pigs (Landrace x Duroc) were used, distributed based on their initial PV as light (15.2 kg), medium (18.2 kg) and heavy (21.3 kg), to identify the muscle in which the abundance of mRNA encoding the Type IIB isoform of myosin heavy chain reflects pig muscle growth. In the light and medium, the Semimembranosus (SM) was the heaviest muscle, but in the heavy the Biceps femoralis (BF) was the heaviest (P < 0.01). In light pigs, the higher expression of RNAm IIB was found in BF and SM (P≤0.05), while the lowest values were for GM, LD and SMT in all pigs. In conclusion, BF and SM are the muscles whose growth is best associated with myosin expression.

Quality changes of Longissimus dorsi and Semimembranosus muscles and perirenal adipose tissue during frozen storage of lambs fed dihydroquercetin or dry distilled rose petals supplemented diet

The aim of study was to determine the changes of frozen stored of Longissimus dorsi and Semimembranosus muscles and perirenal adipose tissue from Bulgarian Dairy Synthetic population lambs, fed a diet supplemented by 7.5 mg dihydroquercetin (DQ) or 545 mg dry distilled rose petals (DDRP)/kg life weight/d. Three groups of 10 male lambs aged 65 days were fed 50 days ad libitum: a control (ground alfalfa + granular compound feed) and two experimental groups (the same diet + the addition of phytonutrients). Samples were collected 1 d post mortem. A half of them were analyzed immediately. Another half was vacuum-packed, quickly frozen at -40°C and stored for 365 d at -18°C. Supplementing of the lamb’s diet with polyphenol-rich phytonutrients reduces with 6.9 -12.9% the aerobic plate count, 9.3 - 25.3% the α-aminoacidic nitrogen, 31.8 - 45.9% the protein carbonyls and 38.9 - 63.4% TBARS (p ≤ 0.05) more pronounced in the m. Longissimus dorsi from lambs fed with 545 mg DDRP/kg/d. Further studies are needed to understand whether the use of higher doses of DDRP or DQ as supplements in the small ruminant’s diet will be able to provide a more pronounced inhibitory effect on oxidation processes in frozen lamb.

FP.31 ANXA11 related adult-onset muscular dystrophy in Greek families

Pathogenic mutations in the low complexity domain of many RNA binding proteins have been reported in both neurogenic and myopathic disorders. Frequent involvement of multiple organs in patients often leads to associated diseases classified as multisystem proteinopathies (MSP) because of the shared aggregate pathology in the affected tissues. These mutations and associated molecular pathomechanisms are part of the dysfunctional cellular clearance of protein aggregates and stress responses. Interestingly, the same causative variant has often been observed in patients with ALS and large families with primary myopathies with or without ALS. Recently, we identified the c.118G>T; p.D40Y variant in <i>ANXA11</i> (NM_0145869.1) segregating with an autosomal dominant myopathy phenotype in five large Greek families from the relatively isolated island of Aegean Sea. However, this variant has been reported in French ALS patients and Brazilian MSP families with ALS patients. In our cohort, none of the patients have an ALS phenotype or family history of ALS. The age of onset in our patients was in the 3^rd-5^th decade of life. Muscle MRI shows myopathic fatty degeneration-replacement of adductors, semitendinosus, semimembranosus, and gastrocnemius muscles. However, none of our patients report any axial involvement with head drop described in the Brazilian MSP families with the same mutation. We present strong genetic evidence that <i>ANXA11</i> p.D40Y causes the observed phenotype in the Greek families. Further studies will unravel the additional molecular factors that can explain the involvement of different target organs causing either ALS or the primary myopathy phenotype.