Semiautomatic Device Research Articles

Small atlas of residual stresses trajectories during surfacing or how to make steel ‘transparent’

ABSTRACT The article shows the unique capabilities of the physical non-destructive magnetoelastic method by the example of creating a small atlas of the principal stresses trajectories fields (isostat) during electric arc deposition. The object of the research is two plates from steel St3 with dimensions of 500x400x8 and 400x200x8 mm, attached along the edges. Six deposition variants are carried out on their surface using a semiautomatic device in a carbon dioxide environment: ‘Parallel rolls’, ‘Circles’, ‘Fork’, ‘Arcs’, ‘Cross’, ‘Angle’, imitating common weld seams. The work presents the sequence of the isostat detection process: drawing a coordinate net on a sample, determining the inclination angles values of tangents related to the trajectories of the greatest principal stresses in the net knots, graphic constructions. The magnetoelastic method is implemented through the use mechanical stress metre – MSM-4 M with a sensor base of 5 mm, an operating frequency of 1000 Hz, an inclinometer error of ±2 degrees. An atlas of typical isostat fields is created on the basis of control at 1900 net knots on the surface of a compact steel sample. Visualization of power flows is provided, which makes the steel appear to be ‘transparent’ and opens up additional opportunities for researchers and production engineers to optimize deposition and welding technologies, and to evaluate the effectiveness of reducing residual stresses in various ways.

Transcriptomic Basis of Serum Resistance and Virulence Related Traits in XDR P. aeruginosa Evolved Under Antibiotic Pressure in a Morbidostat Device.

Colistin is a last resort antibiotic against the critical status pathogen Pseudomonas aeruginosa. Virulence and related traits such as biofilm formation and serum resistance after exposure to sub-inhibitory levels of colistin have been underexplored. We cultivated P. aeruginosa in a semi-automated morbidostat device with colistin, metronidazole and a combination of the two antibiotics for 21 days, and completed RNA-Seq to uncover the transcriptional changes over time. Strains became resistant to colistin within this time period. Colistin-resistant strains show significantly increased biofilm formation: the cell density in biofilm increases under exposure to colistin, while the addition of metronidazole can remove this effect. After 7 days of colistin exposure, strains develop an ability to grow in serum, suggesting that colistin drives bacterial modifications conferring a protective effect from serum complement factors. Of note, strains exposed to colistin showed a decrease in virulence, when measured using the Galleria mellonella infection model. These phenotypic changes were characterized by a series of differential gene expression changes, particularly those related to LPS modifications, spermidine synthesis (via speH and speE) and the major stress response regulator rpoS. Our results suggest a clinically important bacterial evolution under sub-lethal antibiotic concentration leading to potential for significant changes in the clinical course of infection.

Evaluation of Newly Developed Easy-Open Assistive Devices for Pneumatic Tube System Carriers for the Reduction of Work-Related Musculoskeletal Disorders.

Musculoskeletal disorders may affect labor efficiency, cause disability, impair one's work ability, and lower one's quality of life. This consequently leads to a larger expenditure of medical resources. We aimed to design easy-to-open assistive devices for pneumatic tube systems to improve ergonomics and reduce musculoskeletal complaints of workers. We followed a design control process, including designs of motors, gears, sensors, and V-shaped connecting rods. Efficacy was evaluated by examining risks based on job strain index, user satisfaction, and musculoskeletal complaints of operators before and after the system's implementation on a Nordic musculoskeletal questionnaire. We designed three assistive devices: two semiautomatic and one automatic. Each semiautomatic device costs about 300 US dollars and required space of 10 × 18 × 38 cm3. The automatic device costs about 3000 US dollars and required space of 28 × 38 × 50 cm3. The job strain index score decreased from 36 (very high risk) to 3 (low risk) with the semiautomatic devices and to 0 with the automatic device. Musculoskeletal complaints in the neck and upper limbs were reduced, with a significantly higher satisfaction rate for female operators. Our novel design of an automatic cap opening device for a pneumatic tube system was effective in improving ergonomics and reducing musculoskeletal complaints.

A novel method for processing adipose-derived stromal stem cells using a closed cell washing concentration device with a hollow fiber membrane module

Cell-assisted lipotransfer (CAL) is an advanced lipoinjection method that uses autologous lipotransfer with addition of a stromal vascular fraction (SVF) containing adipose-derived stromal stem cells (ASCs). The CAL procedure of manual isolation of cells from fat requires cell processing to be performed in clean environment. To isolate cells from fat without the need for a cell processing center, such as in a procedure in an operation theater, we developed a novel method for processing SVF using a closed cell washing concentration device (CCD) with a hollow fiber membrane module. The CCD consists of a sterilized closed circuit, bags and hollow fiber, semi-automatic device and the device allows removal of >99.97% of collagenase from SVF while maintaining sterility. The number of nucleated cells, ASCs and viability in SVF processed by this method were equivalent to those in SVF processed using conventional manual isolation. Our results suggest that the CCD system is as reliable as manual isolation and may also be useful for CAL. This approach will help in the development of regenerative medicine at clinics without a cell processing center.

Optimization of Adsorbent Layer Type and Developing Solvent in Coccidiostats Sample Preparation with Procedure of Solvent Front Position Extraction.

Coccidiostats are drugs used against coccidiosis, a common disease among breeding animals. Their widespread application leads to the appearance of their residues in food, which is potentially harmful for human health and life. The European Union has established limits of concentrations of these drugs in premixtures and food. Nowadays, there are many methods for monitoring coccidiostats’ presence in market products, but their frequent weakness is sample preparation. Solvent Front Position Extraction is a planar chromatography-based sample preparation method that allows for effective assay of samples with coccidiostats when coupled with LC-MS/MS. The purpose of this research was to find common conditions for the effective isolation of eight coccidiostats from biological sample components with both lower and higher retention than the substances of interest. The acquired results were used for effective isolation of monensin and salinomycin from the premixture samples and allowed for their quantitative determination. The application of a semi-automatic device for the development of chromatograms positively impacted the results, confirming the effectiveness of the method for determining coccidiostats in biological samples.

Prevalence and Determinants of Masked Hypertension in Nigeria: The REMAH Study.

Estimating the burden of hypertension in Nigeria hitherto relied on clinic blood pressure (BP) measurement alone. This excludes individuals with masked hypertension (MH), i.e., normotensive clinic but hypertensive out-of-clinic BP. In a nationally representative sample of adult Nigerians, we obtained clinic BP using auscultatory method and out-of-clinic BP by self-measured home BP with semi-automated oscillometric device. Clinic BP was average of 5 consecutive measurements and home BP was average of 3 days duplicate morning and evening readings. MH was clinic BP <140 mm Hg systolic and 90 mm Hg diastolic and home BP ≥135 mm Hg systolic and/or 85 mm Hg diastolic. Among 933 participants, the prevalence of sustained hypertension, MH, and white-coat hypertension was 28.3%, 7.9%, and 11.9%, respectively. Among subjects whose clinic BP were in the normotensive range (n = 558), the prevalence of MH was 13%; 12% among untreated and 27% among treated individuals. The mutually adjusted odds ratios of having MH among all participants with normotensive clinic BP were 1.33 (95% confidence interval, 1.10-1.60) for a 10-year higher age, 1.59 (1.09-2.40) for a 10 mm Hg increment in systolic clinic BP, and 1.16 (1.08-1.28) for a 10 mg/dl higher random blood glucose. The corresponding estimates in the untreated population were 1.24 (1.03-1.51), 1.56 (1.04-2.44), and 1.16 (1.08-1.29), respectively. MH is common in Nigeria and increasing age, clinic systolic BP, and random blood glucose are the risk factors.

Development of a Semiautomatic Dura Mater Suturing Device for Preventing Cerebrospinal Fluid Leakage in Transsphenoidal Surgery.

Dural suturing in transsphenoidal surgery requires well-honed technical skills. We have developed a semiautomatic dural suturing device and confirmed its effectiveness by comparing it with the conventional method. This device significantly shortens the suturing time compared with the conventional method. The dural suturing time in transsphenoidal surgery could be decreased significantly by use of this novel device.

Development of safe semi-automatic and economic blood cross matching using image processing

Abstract Naming the Blood group is an imperative test for blood transfer and innumerable medical intervention. Generally, the medical practitioner confirms blood typing and its cross-matching suitability before transferring the blood from a person to another. Crossmatch is a fundamental test and is required for wherever blood transfer is needed. While handling an accident case or some emergency, the technicians are performing this test in an open environment which may affect the process and sometimes harmful for technicians. In many hospitals and laboratories, these kinds of tests are carried out in manual using micro plate and test tube. It is time-consuming, error-prone and sometimes it ends up with disease infection for laboratory technicians. Moreover, there is no possibility to revisit the same test results because once after marking it they clean the entire test outcomes. To mitigate the above issues, we propose to design and develop semi-automatic and economical blood cross-matching identification device. This device provides the cross-matching results based on the bonding and its image morphology of the captured image. Further, it provides a captured image for further reference.

Implementing a community-level intervention to control hypertensive disorders in pregnancy using village health workers: lessons learned

IntroductionHypertensive disorders in pregnancy result in about 76,000 maternal deaths per year worldwide. Pre-eclampsia and eclampsia cause the most deaths. Interventions for managing these disorders are available in health facilities. We assess the effect of monitoring pregnant women’s blood pressure (BP) in their homes using village health workers (VHWs) equipped with a BP-measuring device on hypertension in pregnancy, in a resource-poor setting. Also, we assess the VHWs’ competence with the BP device, acceptability and appropriateness of the intervention, and factors that affect the implementation of the intervention.MethodThis is a mixed method study comprising quantitative and qualitative data collection. We implemented the intervention over 6 months across three local government areas in Gombe state, northeast Nigeria. The Replicating Effective Program (REP) framework guided the development of the implementation strategy. The quantitative data include routine measurement of pregnant women’s blood pressure and observation of 118 VHW-client interactions. The routine data collection occurred between February and June 2019, and the observation occurred in January and June 2019. The qualitative data collection occurred via six focus group discussions with VHWs and ten in-depth interviews with community health extension workers in June 2019. We analyzed the data from the quantitative arm with SPSS version 23. For the qualitative arm, we transcribed the audio files, coded the texts, and categorized them using thematic analysis.ResultNine thousand pregnant women were recruited into the program. We observed a significant reduction in the prevalence of hypertension in pregnancy from 1.5 to 0.8% (Z = 4.04; p < 0.00001) after starting the program. Also, we found that VHWs can assess pregnant women’s BP using a semi-automatic BP-measuring device. The intervention is acceptable and appropriate in resource-poor settings. Poor payment of VHW stipend and cooperation of local health staff are barriers to sustaining the intervention.ConclusionIn resource-poor settings, health systems can train and equip non-technical people to identify and refer cases of high blood pressure in pregnancy to local health facilities on time. This may contribute to reducing maternal mortality and morbidity in these settings.

Solvent front position extraction with semi-automatic device as a powerful sample preparation procedure to quantitatitation of tryptophan in human plasma

In the paper the results of the tryptophan determination in human plasma samples prepared with the novel Solvent Front Position Extraction (SFPE) technique are presented. The SFPE procedure is used for preparation of real biological sample for the first time. The results obtained using SFPE are compared with those using the classical sample preparation procedure. Under the optimal conditions, tryptophan and its internal standard were separated from other plasma compounds (matrix) as a small common zone/spot on a chromatographic plate using semiautomatic device equipped with moving pipet, which distributed developing solvent on the adsorbent layer. Tryptophan and the internal standard were evenly distributed within the small common zone from that the both substances were extracted and the solution obtained was transferred to quantitation with LC–MS and MS techniques. The determination results are satisfactory, the percentage values of relative error and RSD relative standard deviation do not exceed 5%. The procedure is characterized by simplicity, high analysis throughput, very good sample purification and seems to be easy applicable to other biological samples with these advantages mentioned.

A positive pressure workstation for semi-automated peptide purification of complex proteomic samples.

High-throughput reliable data generation has become a substantial requirement in many "omics" investigations. In proteomics the sample preparation workflow consists of multiple steps adding more bias to the sample with each additional manual step. Especially for label-free quantification experiments, this drastically impedes reproducible quantification of proteins in replicates. Here, a positive pressure workstation was evaluated to increase automation of sample preparation and reduce workload as well as consumables. Digested peptide samples were purified utilizing a new semi-automated sample preparation device, the Resolvex A200, followed by nanospray liquid chromatography/electrospray ionization (nLC/ESI) Orbitrap tandem mass spectrometry (MS/MS) measurements. In addition, the sorbents Maestro and WWP2 (available in conventional cartridge and dual-chamber narrow-bore extraction columns) were compared with Sep-Pak C18 cartridges. Raw data was analyzed by MaxQuant and Perseus software. The semi-automated workflow with the Resolvex A200 workstation and both new sorbents produced highly reproducible results within 10-300 μg of peptide starting material. The new workflow performed equally as well as the routinely conducted manual workflow with similar technical variability in MS/MS-based identifications of peptides and proteins. A first application of the system to a biological question contributed to highly reliable results, where time-resolved proteomic data was separated by principal component analysis (PCA) and hierarchical clustering. The new workstation was successfully established for proteolytic peptide purification in our proteomic workflow without any drawbacks. Highly reproducible results were obtained in decreased time per sample, which will facilitate further large-scale proteomic investigations.

Semi‐automatic intraosseous device (EZ‐IO) in a paediatric emergency department

Intraosseous access is an alternative to conventional intravenous access. A number of studies have demonstrated the utility and safety of EZ-IO, a recently introduced semi-automatic intraosseous device. However, few studies have investigated the use of EZ-IO in the paediatric emergency setting. The aim of this study was to describe the epidemiological characteristics EZ-IO use including complications among paediatric patients in a paediatric emergency department. We conducted a retrospective descriptive study enrolling children younger than 16 years who visited our emergency department between January 2013 and August 2018. Data on age, gender, diagnosis, insertion success rate and complications were collected and analysed. Seventy-two patients were enrolled; of these 38 (52.8%) were male. The median age was 9 months (interquartile range: 3-34.5 months). Of the total, 22 (30.6%) received more than one IO insertion. Ninety-seven consecutive IO insertions were identified. The rate of successful insertions was achieved at 92.7% (90 insertions). There were 21 complications (21.6%), including 17 extravasations (17.5%) of fluid and four dermal abrasions (4.1%). We found no cases of complications that may have compromised patient safety. The success rate of the EZ-IO insertion was high in paediatric patients. However, we found a higher incidence of dermal abrasions, which might be a complication specific to children.

The Influence of Finish Treatment on the Service Life of the Bearing

Roughness in rolling bearings plays a very important role. Improving the reliability of rubbing parts of equipment is an urgent task. Finishing, superfinishing and laping were used as finishing operations. (Research purpose) The research purpose is in investigating the effect of various finishing operations on the durability of the needle bearing of the cardan joint. (Materials and methods) Needle bearings of the GPP 704702K model (with a nominal dynamic load capacity of 160 kilonewtons) were studied. Tests were carried out on a test rig for four cups and spikes of bearings made of 20KhVG high-carbon chromium bearing steel. The article presents the results of measuring the roughness in the axial direction of the spike and the working surface of the bearing, carried out using a T1000 Basic portable semi-automatic device for measuring surface roughness of first accuracy class. (Results and discussion) As the roughness of the raceway increases, the bearing life decreases. It has been noted a difference in the increasing bearing temperature depending on the roughness of the raceway surface and finishing methods. The sample subjected to superfinishing or laping had the smallest temperature increase and there was a tendency to increase the bearing temperature as the surface roughness increased. (Conclusions) The roughness of the raceway has a close relationship with the service life, with a decrease in roughness, the service life increases by about 1.8 times. It was found that superfinishing or lapping with a sand paper after finishing to a certain roughness nmach = 3 micrometers by grinding is more effective than processing a smooth surface by grinding alone. It was found that when the surface roughness of the raceway decreases, the bearing temperature decreases.

Ustroystvo poluavtomaticheskoy probopodgotovki dlya analiza sledov berilliya v vozduche rabochego pomescheniya s pomosch'yu mass-spektrometrii ERIAD (elektrosprey)

Ustroystvo poluavtomaticheskoy probopodgotovki dlya analiza sledov berilliya v vozduche rabochego pomescheniya s pomosch'yu mass-spektrometrii ERIAD (elektrosprey)

P183 Arterial stiffness was inversely associated with left ventricular diastolic function in indigenous Papuan populations

Abstract Background Age-related increase in systolic pressure is known to be absent in indigenous Papuan populations, partially because of their life-long low-sodium and high-potassium diets. Purpose The aim of this study was to investigate the age-related change in left ventricular (LV) systolic and diastolic function and the relationship with arterial hemodynamics among the indigenous Papuan highland people. Methods Indices of arterial hemodynamics were measured using oscillometric brachial cuff-based sphygmomanometer (Mobil-O-Graph). Brachial-ankle pulse wave velocity (baPWV) was measured using a semiautomatic device (form ABI/baPWV). LV systolic and diastolic function was evaluated by 2-dimensional echocardiography and tissue-Doppler imaging using Vivid iq. Results A total of 81 native Papuans (median age 42 years, 47% women) were enrolled in this study. None of the subjects took any medicines, particularly antihypertensive drugs. All participants presented in sinus rhythm. With ageing, LV mass index remained unchanged, and LV ejection fraction increased, but mildly reduced (40–50%) in 3 participants. Transmitral E-wave velocity slightly decrease but the change was not significant. Transmitral A-wave velocity markedly increased (ρ=0.60, P &amp;lt; 0.001) and E/A ratio decreased with age (ρ=-0.52, P &amp;lt; 0.001). Age-related change of E-wave deceleration time was not significant. Septal e′ (ρ=-0.60, P &amp;lt; 0.001) and lateral e′ decreased (ρ=-0.53, P &amp;lt; 0.001) and average E/e′ increased with age (ρ=0.44, P &amp;lt; 0.001). Left atrial volume index did not change significantly with age. In multivariate regression analysis, baPWV was independently associated with E/e" ratio both before and after adjustment for age, sex, body mass index, mean arterial pressure, and heart rate; however, indices of wave reflection including augmentation index and reflection magnitude were not associated with E/e′. Only age and heart rate were inversely associated with E/A ratio. Conclusion LV systolic function was preserved in indigenous Papuan populations; however, LV diastolic function decreased with ageing. Age-related arterial stiffening, not wave reflection, was inversely related to LV diastolic function.

PELATIHAN BERKESINAMBUNGAN PEMBUATAN ALAT PERANGKAP HAMA SEMI OTOMATIS TANAMAN HORTIKULTURA UNTUK PENINGKATAN PRODUKTIVITAS PETANI KECAMATAN KOTA GAJAH, LAMPUNG TENGAH

Serangga hama pada umumnya menyerang tanaman hortikultura dan membawa virus yang berbahaya bagi tanaman. Untuk itu, diperlukan sistem kendali yang tangguh dan presisi agar mampu mengendalikan serangga hama. Peneliti dari Universitas Lampung telah merancang sebuah alat perangkap serangga hama semi otomatis memanfaatkan energi cahaya matahari yang dapat mengendalikan perkembangan serangga hama di lahan budidaya tanaman hortikultura. Teknologi yang ditransformasikan pada masyarakat memiliki biaya perakitan yang terjangkau dengan manfaat yang besar untuk mengurangi pengaruh kegagalan panen dari serangan serangga hama. Tujuan pengabdian ini adalah transformasi teknologi melalui sosialisasi, pelatihan, pembuatan, perawatan perangkap serangga hama semi otomatis dari Universitas Lampung secara berkesinambungan. Pengabdian ini dilakukan untuk produsen tanaman hortikultura pada kelompok tani Maju, Kecamatan Kota Gajah Lampung Tengah. Sistem kerja alat ini Pada saat malam hari, lampu ultraviolet dan lampu TL yang terpasang mampu menarik serangga hama untuk datang di sekitar lampu untuk jatuh ke perangkap. Sedangkan siang hari, aroma atraktan dan fermomon mampu menarik serangga hama untuk terjerat. Teknologi yang digunakan ini telah terintegrasi dengan sumber listrik dari energi terbarukan yaitu solar cell.

FLOW MEDIATED VASODILATION/VASOCONSTRICTION IN DIFFERENT DISEASE POPULATIONS

Objective: Flow-mediated vasodilation (FMD) has become one of the most widely assessed parameter to analyze endothelial function. However, some techniques used to measure FMD have limitations. Recently, finger plethysmography (EndoPAT) failed to show any kind of reproducibility. A semi-automatic device called UNEX has been developed, which overcomes the limitation of classic systems (in particular the investigator-dependency of conventional, “hand-held” FMD measurements). We report about our first experience by using this device.Design and method: UNEX is a semi-automatic ultrasound system using an H-type ultrasound probe. This system comprised a 7.5-MHz linear array transducer and a novel stereotactic probe-holding device (UNEX EF 18G; UNEX Co., Nagoya, Japan). Another advantage of this device is the continuous recording of B-mode images and A-mode waves of the artery in the longitudinal plane, so that continuous measurement of arterial diameter in the 4.5 min following cuff deflation occurs. Finally, this semi-automatic assessment of FMD has been shown to improve the precision and repeatability of the measurement. FMD and flow mediated vasoconstriction (L-FMC) were measured in patients with heart failure, patients with estimated glomerular filtration rate less and more than 60 ml/min/1,73m2 and healthy subjects using UNEX system. Results: 67% of patients without nephropathy were patients with type 2 diabetes and 33% with hypertension. 22% of patients with nephropathy were on dialysis and 78% had an eGFR less than 60 ml/min/1,73m. Compared to other disease population patients with heart failure were older and healthy population was younger. Conclusions: Our data reveal FMD- and L-FMC values in 3 different disease populations. Updated values on FMD and L-FMC and more detailed analysis will be presented at the meeting.

Validation of a semi automatic device to standardize quantification of Colony-Forming Unit (CFU) on hematopoietic stem cell products

Accurate characterization of hematopoietic stem cells (HSC) products is needed to better anticipate the hematopoietic reconstitution and the outcome in patients. Although CD34+ viable cells enumeration is a key predictor of time to correction of aplasia, it does not fully inform about functionality of cells contained in the graft. CFU assay is the gold standard in vitro potency assay to assess clonogenicity of HSC and consists on the count and identification of colonies several days after culture in a semi solid media. Manual count of colonies with optic microscope is the most commonly used method but its important variability and subjectivity hinders the universal implementation of this potency assay. The aim of this study is to validate a standardized method using the STEMvision™ system, the first semi-automated instrument for imaging and scoring hematopoietic colonies, according to French and European recommendations. Results obtained highlight better performance criteria with STEMvision™ system than the manual method. This semi-automatic device tends to reduce the coefficients of variation of repeatability, inter-operator variability and intermediate precision. This newly available platform could represent an interesting option, significantly improving performances of CFU assays used for the characterization of hematopoietic progenitors.

A Semiautomatic Device for Optic Fiber Manipulation for Laser Proctology

The aim of the present work was to develop a device for semiautomatic manipulation of an optical light guide for laser proctology solving the problem of increasing the accuracy of light guide positioning in low-invasive proctological laser operations using a laser coagulator for the treatment of vascular and oncological diseases. The proposed technical solution increases positioning accuracy by manipulating the optic light guide with a device with two degrees of freedom, firmly attached to the proctoscope, with adjustment of the angle using a lever mechanism and linear semiautomatic movement of the optical light guide using stepper drives. Preclinical and subsequent clinical trials of the device for laser coagulation are planned.

Diagnosis of feline filariasis assisted by a novel semi-automated microfluidic device in combination with high resolution melting real-time PCR

BackgroundThe diagnosis of filariasis traditionally relies on the detection of circulating microfilariae (mf) using Giemsa-stained thick blood smears. This approach has several limitations. We developed a semi-automated microfluidic device to improve and simplify the detection of filarial nematodes.MethodsThe efficiency and repeatability of the microfluidic device was evaluated. Human EDTA blood samples were ‘spiked’ with B. malayi mf at high, moderate, and low levels, and subsequently tested 10 times. The device was also used for a field survey of feline filariasis in 383 domesticated cats in an area of Narathiwat Province, Thailand, the endemic area of Brugia malayi infection.ResultsIn the control blood arbitrarily spiked with mf, the high level, moderate level and low level mf-positive controls yielded coefficient variation (CV) values of 4.44, 4.16 and 4.66%, respectively, at the optimized flow rate of 6 µl/min. During the field survey of feline filariasis in Narathiwat Province, the device detected mf in the blood of 34 of 383 cats (8.9%) whereas mf were detected in 28 (7.3%) cats using the blood smear test. Genomic DNA was extracted from mf trapped in the device after which high-resolution melting (HRM) real-time PCR assay was carried out, which enabled the simultaneous diagnosis of filarial species. Among the 34 mf-positive samples, 12 were identified as B. malayi, 15 as Dirofilaria immitis and 7 as| D. repens.ConclusionsWe developed a semi-automated microfluidic device to detect mf of filarial parasites that could be used to diagnose lymphatic filariasis in human populations. This novel device facilitates rapid, higher-throughput detection and identification of infection with filariae in blood samples.