Semisolid Surfaces Research Articles

A semi-solid surface fluorescence method for the determination of lactate dehydrogenase

A semi-solid surface fluorescence method for the determination of lactic dehydrogenase (LDH) is reported. The commercially available enzyme in aqueous solution, and the enzyme in blood serum were studied. The serum analysis is compared to a method described by Bergmeyer . As little as 160 units of LDH per ml of blood serum can be determined with an accuracy of better than 3%. The reaction system used was : [Display omitted] The rate of production of the fluorescent product NADH was monitored. An investigation of pad color and pad stability was also performed.