BACKGROUND One of the most widely used methods to record horizontal condylar guidance is the use of protrusive interocclusal records. Therefore, this study was done to evaluate the reliability of protrusive interocclusal record with and without the influence of anterior determinant on horizontal condylar guidance among dentulous patients. METHODS Diagnostic impressions of 21 participants were made and casts were poured. Facebow record were obtained and transferred to semi-adjustable articulator. Protrusive interocclusal records were made using Alu-wax. In group 1, protrusive interocclusal records were obtained at incisal edge to edge position (conventional method). In group 2, 3 and 4 protrusive interocclusal records were obtained at a distance of 4 mm, 6 mm and at incisal edge to edge position respectively using a customized flat anterior jig. Programming of the Hanau articulator were done for all the 4 groups. One-way ANOVA test and Pearson’s correlation tests were done to compare and correlate horizontal condylar guidance. RESULTS The mean values obtained for flat anterior jig at incisal edge to edge position, at 4 mm and 6 mm provided good Pearson correlation values for the right (R = 0.773, R = 0.779, R = 0.632) as well as the left side (R = 0.631, R = 0.601, R = 0.545) respectively, on comparison with the conventional incisal edge to edge values. This indicated a positive correlation which was statistically significant. CONCLUSIONS The horizontal condylar guidance is influenced by anterior determinant. Therefore, the reliability of protrusive records is dependent on eliminating the influence of anterior determinant. KEY WORDS Horizontal Condylar Guidance, Anterior Determinant, Protrusive Interocclusal Records