Frequency Of Semen Collection Research Articles

Frequency of Semen Collection in Relation to Semen Volume, Sperm Concentration and Fertility in the Chicken

Data on frequency of semen collection were evaluated over a 13-week period for broiler breeder-type males, and over a 20-week period for Leghorn males. Semen was collected from the broiler-type males daily, twice daily, and three times weekly and from Leghorn males weekly, three times weekly, and five times weekly. Semen volume and sperm cell numbers were determined each week for the various treatments. In the broiler breeders, the males that were ejaculated three times weekly produced a significantly greater semen volume, and more sperm cells per ejaculation than did the males in any of the other treatments. In the Leghorns, males that were ejaculated three times weekly produced a significantly greater semen volume and had a higher cell concentration than the males that were ejaculated five times weekly. Leghorn males that were ejaculated once weekly had sperm concentrations equal to those of males that were ejaculated three times weekly, and had significantly more sperm cells than those ejaculated five times weekly. Frequency of collection, within the ranges studied in either broiler breeders or Leghorns, apparently had no effect on fertility.It was concluded from these studies that the frequency of semen collection had a definite effect on semen volume and sperm cell numbers, but not on fertility. Sperm cell numbers varied considerably within treatments; however, all treatments did contain the minimum number of sperm cells recommended for optimum fertility.

Free Amino Acids and Amino Compounds in Bovine Seminal Plasma

Factors affecting free amino acid and amino compound content of bovine seminal plasma and the relationship with fertility, motility, and spermatozoa concentration were studied. The effect of addition of free amino acids to semen extenders on spermatozoa metabolism also was studied. The sulfosalicylic method of sample preparation yielded a higher recovery rate of free amino acids than did the picric method. Samples centrifuged immediately after collection and stored at −196C until preparation for analysis yielded more consistent results than with four other methods investigated. Amino acid content of samples declined significantly with four consecutive semen collections. Decreasing frequency of semen collection from weekly to biweekly to triweekly also significantly decreased amino acid concentration. Twenty free amino acids and amino compounds were identified in bovine seminal plasma and 29 other amino compounds were detected. Glutamic acid provided 50% of the 8.55μmoles/ml total free amino acids quantitated. No amino acids were correlated significantly with fertility. Methionine sulfoxide, valine, ethanolamine, and arginine were positively correlated, whereas half-cystine was negatively and highly correlated to motility of spermatozoa. No change in spermatozoa metabolism was found due to addition of five free amino acids to semen extenders.

Effect of Frequency of Semen Collection on Fertility, Hatchability and Spermatozoa Concentration in the Turkey

Effect of Frequency of Semen Collection on Fertility, Hatchability and Spermatozoa Concentration in the Turkey

Effect of Frequency of Semen Collection on Fertility, Hatchability and Spermatozoa Concentration in the Turkey

ABSTRACT THE increased interest in the practice of artificial insemination by turkey hatching egg producers to improve the reproductive efficiency of their flocks has resulted in many unanswered problems. These problems must be solved in order to increase the efficiency of artificial breeding and to make sound recommendations to flock owners employing this practice in their breeding program. Since the volume of semen obtained from turkey males at any one time is relatively small, varying from barely measurable amounts to slightly more than 0.3 ml. (Carson et al., 1955; and McCartney, 1956), information was needed to determine the effect of collection frequency on quantity of semen and fertilizing capacity. Lorenz et al. (1955) studied the effect of collection frequency on the quantity of semen produced by turkey males and found that the volume of semen decreased gradually during the first week of daily collection; however, this decrease in volume had no . . .

The sperm production of adult rams in relation to frequency of semen collection

Many estimates have been made of sperm production in various species, but in most cases examinations have been made infrequently or with irregular intervals between successive collections. Since during a period of sexual rest spermatozoa may accumulate in the epididymis, or with longer periods may undergo disintegration and absorption in the vas and ampulla (Simeone & Young, 1931), the number ejaculated at an particular collection will not necessarily reflect the level of sperm production in the testis. Moreover, there is the possibility that copulation may actually stimulate spermatogenesis. In horses, Chang (1943) found that more sperms were ejaculated if collections were made frequently and at regular intervals. In the present experiments an attempt has been made to determine the effect of frequency of collection upon the sperm production of rams, the collections being taken at regular intervals and for a definite period, in this case ten days.