Temperature stress has a significant impact on the structural stress of (super) large-span tunnel lining, which can easily lead to structural fatigue damage and premature cracking. With the increasing scale and quantity of super large-span tunnels, the issue of temperature stress in secondary lining has attracted widespread attention. Previous studies have paid little attention to the influence of temperature stress on the structural internal forces of ordinary small–medium-span tunnels, but this influence cannot be ignored for super large-span tunnels. We take the Letuan Tunnel (a double-hole eight-lane tunnel) of the Binzhou-Laiwu expressway renovation and expansion project in Shandong Province as a case study and analyze the mechanical response of the secondary lining through on-site measurement. Moreover, a numerical simulation was conducted to evaluate the effects of self-weight and temperature stress on the secondary lining of the case tunnel. The results indicate that: the stress of the secondary lining concrete and steel bars is greatly affected by seasonal temperature changes. The compressive stress of the concrete and steel bars is significantly greater in summer than in winter, and the tensile stress is greater in winter than in summer. Furthermore, multiple measurement points have shown a phenomenon of transition between tensile and compressive stress states. The stress of concrete and steel bars fluctuates periodically with a sine function over time, with a fluctuation period of one year. The structural stress increases with the increase of summer temperature and decreases with the decrease of winter temperature. The fluctuation amplitude of stress in the inner side of the lining concrete and steel bars is greater than that on the outer side. Among them, the stress amplitudes of the inner and outer sides of the concrete are between 0.77–1.75 MPa and 0.44–1.07 MPa, respectively, and the stress amplitudes of the inner and outer steel bars are between 5–31 MPa and 7–13 MPa, respectively. The safety factors in summer are lower than those in winter. The minimum safety factors for secondary lining in summer and winter are 3.4 and 4.6, respectively, which can meet the safety requirements for service. The average axial forces of the secondary lining under the coupling effects of self-weight and temperature in winter and summer are 528 MPa and 563 MPa, respectively, which are significantly greater than the combined axial forces under their individual effects. The bending moment distribution of the secondary lining at the tunnel vault, inverted arch, wall spring and other positions under the coupling effect of self-weight and temperature is different from or even opposite to the bending moment superposition result under the two individual actions. The achieved results reveal that the influence of temperature stress on the service performance of the lining structure cannot be ignored, and the research results can provide useful reference for similar tunnels and related studies.