The influence of neutron irradiation on microstructural evolution and mechanical properties of mono- and poly-crystalline molybdenum and its alloys has been investigated. Tensile specimens and 3 mm diameter discs of monocrystals of pure molybdenum and Mo–5%Re were irradiated with fission neutrons at ~320 K to displacement doses in the range 5.4 × 10 −4 to 1.6 × 10 −1 dpa (NRT) in the DR-3 reactor at Risø National Laboratory. For comparison, polycrystalline specimens of Mo–5% Re and TZM were also irradiated together with the monocrystalline specimens. Both unirradiated and irradiated specimens were tensile tested at 295 K. Post-irradiation microstructures were quantitatively characterized using a transmission electron microscope (TEM). Fracture surfaces were examined in a scanning electron microscope (SEM). The results of tensile testing as well as of transmission and scanning microscopy are presented and discussed in terms of intracascade clustering of self-interstitial atoms and the role of one-dimensional glide of these clusters in controlling microstructural evolution and the resulting mechanical properties.