Summary The active transport of β-alanine (β-Ala) and of γ-amino butyric acid (GABA), contrarily to what is observed with α-amino acids, appears to be an inducible process in Pseudomonas aeruginosa A 237 , strain. The transport of β-Ala is induced not only by β-Ala, but also by GABA, β-amino isobutyric acid (β-AIB), 2-amino ethylphosphonic acid (2-AEPh) and 3-amino propylphosphonic acid (3-APrPh). The three above mentioned carboxylic compounds will induce a single β-Ala transport system, whereas their phosphonic analogues give rise to a biphasic uptake. Whatever inducing molecule experimented, β-Ala uptake is inhibited only by GABA and by β-AIB, either competitively (β-Ala or β-AIB induction) or non competitively (GABA, 2-AEPh or 3-APrPh induction). The transport of GABA is similar to that of β-Ala, the same inductors and inhibitors being observed in both cases ; GABA is however the only compound able to induce a single and strictly specific system. The two β-amino acids, β-Ala and β-AIB, inhibit both low and high affinity self-induced systems ; Dixon plots show a purely competitive inhibition at high GABA concentrations and an hyperbolic competitive inhibition at low GABA concentrations. These β-amino acids also appeared as competitive inhibitors for the high affinity systems which were induced by 2-AEPh and 3-APrPh. Despite the fact that β-Ala and β-AIB, as well as 2-AEPh and 3-APrPh, induce transport systems possessing similar kinetics and specificty, the behaviour of the permease activities towards osmotic shock treatment was different : a severe reduction of β-Ala and GABA entry into the cells was observed only after growth on β-Ala, 2-AEPh and GABA. Our results emphasize the complexity of ω-amino acid uptake in Ps. aeruginosa .