Self-induced Back-action Research Articles

Computational and experimental study of AC measurements performed by a double-nanohole plasmonic nanopore sensor on 20 nm silica nanoparticles

A novel method of AC sensing is presented that uses a double nanohole (DNH) nanoaperture atop a solid-state nanopore (ssNP) to trap analytes and measure their optical and electrical properties. In this method analytes are propelled by an external applied voltage towards the sensor until they are trapped at the DNH-ssNP interface via a self-induced back action (SIBA) plasmonic force. We have previously named this method SIBA Actuated Nanopore Electrophoresis (SANE) sensing and have shown its ability to perform concurrent optical and DC electrical measurements. Here, we extend this method to AC sensing of 20 nm SiO2 (silica) nanoparticles, using voltage modulation over a wide range of frequencies applied on top of a baseline DC bias. The sensor was constructed using two-beam GFIS Focused Ion Beam (FIB) lithography, incorporating Ne FIB to mill the DNH and He FIB to drill a central 30 nm ssNP. We utilized COMSOL Multiphysics simulations to explore the multi-frequency AC current conductance properties of the silica nanoparticles trapped at the SANE sensor. These simulations computed conductance changes and phase shifts induced by the presence of the nanoparticle over an AC frequency range of 20 Hz to 100 kHz. Experimental measurements confirmed the trends seen in the computational data. Additional computational studies were then performed to dissect the underlying mechanisms driving the observed AC measurements. Looking forward, we aim to adapt this technology for probing therapeutic nanoparticles non-invasively, offering a promising tool for enhancing quality control of nanoparticle-mediated drug and gene delivery systems.

Self-induced back-action for aperture trapping: Bethe-Rayleigh theory.

A dielectric (nano)particle can influence the local electromagnetic field and thereby alter its interaction with that field through the process of self-induced back-action. While this phenomenon is usually considered theoretically as a change in a cavity resonance frequency, such theoretical approaches are not as appropriate when considering systems away from resonance, such as with a subwavelength aperture in a metal film. Here we consider the interaction between an aperture, modelled with Bethe theory as a magnetic dipole, and a Rayleigh particle, modelled as an electric dipole. Using this magnetic dipole - electric dipole interaction, we quantify the self-induced back-action of the particle on the aperture transmission and the optical trapping potential. The model shows quantitative agreement with finite-difference time-domain simulations. This shows that the physics of self-induced back-action for an aperture and a nanoparticle can be understood in terms of dipole-dipole coupling.

Resonant optical trapping of Janus nanoparticles in plasmonic nanoaperture

Controlled trapping of light-absorbing nanoparticles with low-power optical tweezers is crucial for remote manipulation of small objects. This study takes advantage of the synergetic effects of tightly confined local fields of plasmonic nanoaperture, self-induced back-action (SIBA) of nanoparticles, and resonant optical trapping method to demonstrate enhanced manipulation of Janus nanoparticles in metallic nanohole aperture. We theoretically demonstrate that displacement of Au-coated Janus nanoparticles toward plasmonic nanoaperture and proper orientation of the metal coating give rise to enhanced near-field intensity and pronounced optical force. We also explore the effect of resonant optical trapping by employing a dual-laser system, where an on-resonant green laser excites the metal-coated nanoparticle, whereas an off-resonant near-infrared laser plays trapping role. It is found that, at the optimum nanoparticle configuration, the resonant optical trapping technique can result in threefold enhancement of optical force, which is attributed to the excitation of surface plasmon resonance in Janus nanoparticles. The findings of this study might pave the way for low-power optical manipulation of light-absorbing nanoparticles with possible applications in nanorobotics and drug delivery.

Multi-physics simulations and experimental comparisons for the optical and electrical forces acting on a silica nanoparticle trapped by a double-nanohole plasmonic nanopore sensor

Bimodal optical-electrical data generated when a 20 nm diameter silica (SiO2) nanoparticle was trapped by a plasmonic nanopore sensor were simulated using Multiphysics COMSOL and compared with sensor measurements for closely matching experimental parameters. The nanosensor, employed self-induced back action (SIBA) to optically trap nanoparticles in the center of a double nanohole (DNH) structure on top a solid-state nanopores (ssNP). This SIBA actuated nanopore electrophoresis (SANE) sensor enables simultaneous capture of optical and electrical data generated by several underlying forces acting on the trapped SiO2 nanoparticle: plasmonic optical trapping, electroosmosis, electrophoresis, viscous drag, and heat conduction forces. The Multiphysics simulations enabled dissecting the relative contributions of those forces acting on the nanoparticle as a function of its location above and through the sensor's ssNP. Comparisons between simulations and experiments demonstrated qualitative similarities in the optical and electrical time-series data generated as the nanoparticle entered and exited from the SANE sensor. These experimental parameter-matched simulations indicated that the competition between optical and electrical forces shifted the trapping equilibrium position close to the top opening of the ssNP, relative to the optical trapping force maximum that was located several nm above. The experimentally estimated minimum for the optical force needed to trap a SiO2 nanoparticle was consistent with corresponding simulation predictions of optical-electrical force balance. The comparison of Multiphysics simulations with experiments improves our understanding of the interplay between optical and electrical forces as a function of nanoparticle position across this plasmonic nanopore sensor.

Enabling Self-Induced Back-Action Trapping of Gold Nanoparticles in Metamaterial Plasmonic Tweezers.

The pursuit for efficient nanoparticle trapping with low powers has led to optical tweezers technology moving from the conventional free-space configuration to advanced plasmonic systems. However, trapping nanoparticles smaller than 10 nm still remains a challenge even for plasmonic tweezers. Proper nanocavity design and excitation has given rise to the self-induced back-action (SIBA) effect offering enhanced trap stiffness with decreased laser power. In this work, we investigate the SIBA effect in metamaterial tweezers and its synergy with the exhibited Fano resonance. We demonstrate stable trapping of 20 nm gold particles with trap stiffnesses as high as 4.18 ± 0.2 (fN/nm)/(mW/μm2) and very low excitation intensity. Simulations reveal the existence of two different groups of hotspots on the plasmonic array. The two hotspots exhibit tunable trap stiffnesses, a unique feature that can allow for sorting of particles and biological molecules based on their characteristics.

Self-induced backaction in optical waveguides.

In this paper, we study the backaction effect on the force exerted upon Rayleigh particles in guided structures. We show that the backaction becomes stronger as the group velocity of the guided modes is decreased, which is not unexpected since the fall of group velocity increases the interaction time between the particle and the electromagnetic field. Interestingly, the sign of the group velocity affects the pushing and pulling nature of the exerted electromagnetic force. We specifically investigate the case of a single mode optical waveguide both in the propagating and evanescent regimes, and show that the backaction enables us to enhance the ratio of the potential depth to the trapping intensity, and thereby can be a beneficial tool for nondestructive trapping of small nanoparticles. We further show that backaction can induce some resonances in the optical force in the evanescent regime. These resonances can be employed for sorting of nanoparticles.

Self-Induced Back-Action Actuated Nanopore Electrophoresis (SANE) Sensor for Label-Free Detection of Cancer Immunotherapy-Relevant Antibody-Ligand Interactions.

We fabricated a novel single molecule nanosensor by integrating a solid-state nanopore and a double nanohole nanoaperture. The nanosensor employs Self-Induced Back-Action (SIBA) for optical trapping and enables SIBA-Actuated Nanopore Electrophoresis (SANE) for concurrent acquisition of bimodal optical and electrical signatures of molecular interactions. This work describes how to fabricate and use the SANE sensor to quantify antibody-ligand interactions. We describe how to analyze the bimodal optical-electrical data to improve upon the discrimination of antibody and ligand versus bound complex compared to electrical measurements alone. Example results for specific interaction detection are described for T-cell receptor-like antibodies (TCRmAbs) engineered to target peptide-presenting Major Histocompatibility Complex (pMHC) ligands, representing a model of target ligands presented on the surface of cancer cells. We also describe how to analyze the bimodal optical-electrical data to discriminate between specific and non-specific interactions between antibodies and ligands. Example results for non-specific interactions are shown for cancer-irrelevant TCRmAbs targeting the same pMHCs, as a control. These example results demonstrate the utility of the SANE sensor as a potential screening tool for ligand targets in cancer immunotherapy, though we believe that its potential uses are much broader.

Quantification of low affinity binding interactions between natural killer cell inhibitory receptors and targeting ligands with a self-induced back-action actuated nanopore electrophoresis (SANE) sensor

A plasmonic nanopore sensor enabling detection of bimodal optical and electrical molecular signatures was fabricated and tested for its ability to characterize low affinity ligand-receptor interactions. This plasmonic nanosensor uses self-induced back-action (SIBA) for optical trapping to enable SIBA-actuated nanopore electrophoresis (SANE) through a nanopore located immediately below the optical trap volume. A natural killer (NK) cell inhibitory receptor heterodimer molecule CD94/NKG2A was synthesized to target a specific peptide-presenting Qa-1b Qdm ligand as a simplified model of low-affinity interactions between immune cells and peptide-presenting cancer cells that occurs during cancer immunotherapy. A cancer-irrelevant Qa-1b GroEL ligand was also targeted by the same receptor as a control experiment to test for non-specific binding. The analysis of different pairs of bimodal SANE sensor signatures enabled discrimination of ligand, receptor and their complexes and enabled differentiating between specific and non-specific ligand interactions. We were able to detect ligand-receptor complex binding at concentrations over 500 times lower than the free solution equilibrium binding constant (K D ). Additionally, SANE sensor measurements enabled estimation of the fast dissociation rate (k off) for this low-affinity specific ligand-receptor system, previously shown to be challenging to quantify with commercial technologies. The k off value of targeted peptide-presenting ligands is known to correlate with the subsequent activation of immune cells in vivo, suggesting the potential utility of the SANE senor as a screening tool in cancer immunotherapy.

Optofluidic Microengine in A Dynamic Flow Environment via Self-Induced Back-Action

Most existing optofluidic particle engines only operate in a static environment. Here, we present a four-energy-state optofluidic microengine that operates stably in a dynamic flow environment, a f...

Detection of specific antibody-ligand interactions with a self-induced back-action actuated nanopore electrophoresis sensor

Recent advances in plasmonic nanopore technologies have enabled the use of concurrently acquired bimodal optical-electrical data for improved quantification of molecular interactions. This work presents the use of a new plasmonic nanosensor employing self-induced back-action (SIBA) for optical trapping to enable SIBA-actuated nanopore electrophoresis (SANE) for quantifying antibody-ligand interactions. T-cell receptor-like antibodies (TCRmAbs) engineered to target peptide-presenting major histocompatibility complex (pMHC) ligands, representing a model of target ligands presented on the surface of cancer cells, were used to test the SANE sensor’s ability to identify specific antibody-ligand binding. Cancer-irrelevant TCRmAbs targeting the same pMHCs were also tested as a control. It was found that the sensor could provide bimodal molecular signatures that could differentiate between antibody, ligand and the complexes that they formed, as well as distinguish between specific and non-specific interactions. Furthermore, the results suggested an interesting phenomenon of increased antibody-ligand complex bound fraction detected by the SANE sensor compared to that expected for corresponding bulk solution concentrations. A possible physical mechanism and potential advantages for the sensor’s ability to augment complex formation near its active sensing volume at concentrations lower than the free solution equilibrium binding constant (KD) are discussed.

Contactless optical trapping and manipulation of nanoparticles utilizing SIBA mechanism and EDL force.

On-chip optical tweezers based on evanescent fields overcome the diffraction limit of the free-space optical tweezers and can be a promising technique for developing lab-on-a-chip devices. While such trapping allows for low-cost and precise manipulation, it suffers from unavoidable contact with the device surface, which eliminates one of the major advantages of the optical trapping. Here, we use a 1D photonic crystal cavity to trap nanoparticles and propose a novel method to control and manipulate the particle distance from the cavity utilizing a self-induced back-action (SIBA) mechanism and electrical-double-layer (EDL) force. It is numerically shown that a 200 nm radius silica particle can be trapped near the cavity with a potential well deeper than 178kBT by 1 mW of input power without any contact with the surface and easily moved vertically with nanometer precision by wavelength detuning.

Self-induced back action actuated nanopore electrophoresis (SANE)

We present a novel method to trap nanoparticles in double nanohole (DNH) nanoapertures integrated on top of solid-state nanopores (ssNP). The nanoparticles were propelled by an electrophoretic force from the cis towards the trans side of the nanopore but were trapped in the process when they reached the vicinity of the DNH-ssNP interface. The self-induced back action (SIBA) plasmonic force existing between the tips of the DNH opposed the electrophoretic force and enabled simultaneous optical and electrical sensing of a single nanoparticle for seconds. The novel SIBA actuated nanopore electrophoresis (SANE) sensor was fabricated using two-beam GFIS FIB. Firstly, Ne FIB milling was used to create the DNH features and was combined with end pointing to stop milling at the metal-dielectric interface. Subsequently, He FIB was used to drill a 25 nm nanopore through the center of the DNH. Proof of principle experiments to demonstrate the potential utility of the SANE sensor were performed with 20 nm silica and Au nanoparticles. The addition of optical trapping to electrical sensing extended translocation times by four orders of magnitude. The extended electrical measurement times revealed newly observed high frequency charge transients that were attributed to bobbing of the nanoparticle driven by the competing optical and electrical forces. Frequency analysis of this bobbing behavior hinted at the possibility of distinguishing single from multi-particle trapping events. We also discuss how SANE sensor measurement characteristics differ between silica and Au nanoparticles due to differences in their physical properties and how to estimate the charge around a nanoparticle. These measurements show promise for the SANE sensor as an enabling tool for selective detection of biomolecules and quantification of their interactions.

Self-Induced Backaction Optical Pulling Force.

We achieve long-range and continuous optical pulling in a periodic photonic crystal background, which supports a unique Bloch mode with the self-collimation effect. Most interestingly, the pulling force reported here is mainly contributed by the intensity gradient force originating from the self-induced backaction of the object to the self-collimation mode. This force is sharply distinguished from the widely held conception of optical tractor beams based on the scattering force. Also, this pulling force is insensitive to the angle of incidence and can pull multiple objects simultaneously.

Fano Resonance-Assisted Plasmonic Trapping of Nanoparticles

Plasmonic optical trapping is widely applied in the field of bioscience, microfluidics, and quantum optics. It can play a vital role to extend optical manipulation tools from micrometer to nanometer scale level. Currently, it is a challenge to obtain the highly stable optical trapping with low power and less damage. In this paper, we propose Fano resonance-assisted self-induced back-action (FASIBA) method, through which a single 40-nm gold particle can be trapped in hole-slit nano-aperture milled on metallic film. It is used to achieve ultra-accurate positioning of nanoparticle, metallic nanostructures at wide infrared wavelength range, quite effectively and evidently. The stable plasmonic trapping is achieved by tuning the transmission wavelengths and modifications of nanoslit, indicating that the depth of potential well can be increased from minus 8KT to 12KT, with the input power of 109 W/m2. This can be attributed to great modifications in Fano resonance transmissions according to self-induced back-action (SIBA) theory. The results are basically helpful to facilitate the trapping with lower power and less damage to the objects, which enables new scenario for the treatment of undesirable spread of a single nanoscale creature, such as virus.

Arrested Dimer’s Diffusion by Self-Induced Back-Action Optical Forces

The diffusion of a dimer made out of two resonant dipolar scatters in an optical lattice is theoretically analyzed. When a small particle diffuses through an optically induced potential landscape, its Brownian motion can be strongly suppressed by gradient forces, proportional to the particle’s polarizability. For a single lossless monomer at resonance, the gradient force vanishes and the particle diffuses as in absence of external fields. However, we show that when two monomers link in a dimer, the multiple scattering among the monomers induces both a torque and a net force on the dimer’s center of mass. This “self-induced back-action” force leads to an effective potential energy landscape, entirely dominated by the mutual interaction between monomers, which strongly influences the dynamics of the dimer. Under appropriate illumination, single monomers in a colloidal suspension freely diffuse while dimers become trapped. Our theoretical predictions are tested against extensive Langevin molecular dynamics s...

Unraveling the optomechanical nature of plasmonic trapping.

Noninvasive and ultra-accurate optical manipulation of nanometer objects has recently gained interest as a powerful tool in nanotechnology and biophysics. Self-induced back-action (SIBA) trapping in nano-optical cavities has the unique potential for trapping and manipulating nanometer-sized objects under low optical intensities. However, thus far, the existence of the SIBA effect has been shown only indirectly via its enhanced trapping performances. In this article, we present the first time direct experimental evidence of the self-reconfiguration of the optical potential that is experienced by a nanoparticle trapped in a plasmonic nanocavity. Our observations enable us to gain further understanding of the SIBA mechanism and to determine the optimal conditions for boosting the performances of SIBA-based nano-optical tweezers.

3D nano-structures for laser nano-manipulation

The resputtering of gold films from nano-holes defined in a sacrificial PMMA mask, which was made by electron beam lithography, was carried out with a dry plasma etching tool in order to form well-like structures with a high aspect ratio (height/width ≈ 3–4) at the rims of the nano-holes. The extraordinary transmission through the patterns of such nano-wells was investigated experimentally and numerically. By doing numerical simulations of 50-nm and 100-nm diameter polystyrene beads in water and air, we show the potential of such patterns for self-induced back-action (SIBA) trapping. The best trapping conditions were found to be a trapping force of 2 pN/W/μm2 (numerical result) exerted on a 50-nm diameter bead in water. The simulations were based on the analytical Lorentz force model.

Optical Trapping of Nanoparticles

Optical trapping is a technique for immobilizing and manipulating small objects in a gentle way using light, and it has been widely applied in trapping and manipulating small biological particles. Ashkin and co-workers first demonstrated optical tweezers using a single focused beam. The single beam trap can be described accurately using the perturbative gradient force formulation in the case of small Rayleigh regime particles. In the perturbative regime, the optical power required for trapping a particle scales as the inverse fourth power of the particle size. High optical powers can damage dielectric particles and cause heating. For instance, trapped latex spheres of 109 nm in diameter were destroyed by a 15 mW beam in 25 sec, which has serious implications for biological matter. A self-induced back-action (SIBA) optical trapping was proposed to trap 50 nm polystyrene spheres in the non-perturbative regime. In a non-perturbative regime, even a small particle with little permittivity contrast to the background can influence significantly the ambient electromagnetic field and induce a large optical force. As a particle enters an illuminated aperture, light transmission increases dramatically because of dielectric loading. If the particle attempts to leave the aperture, decreased transmission causes a change in momentum outwards from the hole and, by Newton's Third Law, results in a force on the particle inwards into the hole, trapping the particle. The light transmission can be monitored; hence, the trap can become a sensor. The SIBA trapping technique can be further improved by using a double-nanohole structure. The double-nanohole structure has been shown to give a strong local field enhancement. Between the two sharp tips of the double-nanohole, a small particle can cause a large change in optical transmission, thereby inducing a large optical force. As a result, smaller nanoparticles can be trapped, such as 12 nm silicate spheres and 3.4 nm hydrodynamic radius bovine serum albumin proteins. In this work, the experimental configuration used for nanoparticle trapping is outlined. First, we detail the assembly of the trapping setup which is based on a Thorlabs Optical Tweezer Kit. Next, we explain the nanofabrication procedure of the double-nanohole in a metal film, the fabrication of the microfluidic chamber and the sample preparation. Finally, we detail the data acquisition procedure and provide typical results for trapping 20 nm polystyrene nanospheres.

Optical Trapping of Nanoparticles

Optical trapping is a technique for immobilizing and manipulating small objects in a gentle way using light, and it has been widely applied in trapping and manipulating small biological particles. Ashkin and co-workers first demonstrated optical tweezers using a single focused beam1. The single beam trap can be described accurately using the perturbative gradient force formulation in the case of small Rayleigh regime particles1. In the perturbative regime, the optical power required for trapping a particle scales as the inverse fourth power of the particle size. High optical powers can damage dielectric particles and cause heating. For instance, trapped latex spheres of 109 nm in diameter were destroyed by a 15 mW beam in 25 sec1, which has serious implications for biological matter2,3. A self-induced back-action (SIBA) optical trapping was proposed to trap 50 nm polystyrene spheres in the non-perturbative regime4. In a non-perturbative regime, even a small particle with little permittivity contrast to the background can influence significantly the ambient electromagnetic field and induce a large optical force. As a particle enters an illuminated aperture, light transmission increases dramatically because of dielectric loading. If the particle attempts to leave the aperture, decreased transmission causes a change in momentum outwards from the hole and, by Newton's Third Law, results in a force on the particle inwards into the hole, trapping the particle. The light transmission can be monitored; hence, the trap can become a sensor. The SIBA trapping technique can be further improved by using a double-nanohole structure. The double-nanohole structure has been shown to give a strong local field enhancement5,6. Between the two sharp tips of the double-nanohole, a small particle can cause a large change in optical transmission, thereby inducing a large optical force. As a result, smaller nanoparticles can be trapped, such as 12 nm silicate spheres7 and 3.4 nm hydrodynamic radius bovine serum albumin proteins8. In this work, the experimental configuration used for nanoparticle trapping is outlined. First, we detail the assembly of the trapping setup which is based on a Thorlabs Optical Tweezer Kit. Next, we explain the nanofabrication procedure of the double-nanohole in a metal film, the fabrication of the microfluidic chamber and the sample preparation. Finally, we detail the data acquisition procedure and provide typical results for trapping 20 nm polystyrene nanospheres.

Enhanced Optical Trapping and Arrangement of Nano-Objects in a Plasmonic Nanocavity

Gentle manipulation of micrometer-sized dielectric objects with optical forces has found many applications in both life and physical sciences. To further extend optical trapping toward the true nanometer scale, we present an original approach combining self-induced back action (SIBA) trapping with the latest advances in nanoscale plasmon engineering. The designed resonant trap, formed by a rectangular plasmonic nanopore, is successfully tested on 22 nm polystyrene beads, showing both single- and double-bead trapping events. The mechanism responsible for the higher stability of the double-bead trapping is discussed, in light of the statistical analysis of the experimental data and numerical calculations. Furthermore, we propose a figure of merit that we use to quantify the achieved trapping efficiency and compare it to prior optical nanotweezers. Our approach may open new routes toward ultra-accurate immobilization and arrangement of nanoscale objects, such as biomolecules.