Internet trolling is considered a negative form of online interaction that can have detrimental effects on people's well-being. This pre-registered, experimental study had three aims: first, to replicate the association between internet users' online trolling behavior and the Dark Tetrad of personality (Machiavellianism, narcissism, psychopathy, and sadism) established in prior research; second, to investigate the effect of experiencing social exclusion on people's motivation to engage in trolling behavior; and third, to explore the link between humor styles and trolling behavior. In this online study, participants were initially assessed on their personality, humor styles, and global trolling behavior. Next, respondents were randomly assigned to a social inclusion or exclusion condition. Thereafter, we measured participants' immediate trolling motivation. Results drawn from 1,026 German-speaking participants indicate a clear correlation between global trolling and all facets of the Dark Tetrad as well as with aggressive and self-defeating humor styles. However, no significant relationship between experiencing exclusion/inclusion and trolling motivation emerged. Our quantile regression findings suggest that psychopathy and sadism scores have a significant positive effect on immediate trolling motivation after the experimental manipulation, whereas Machiavellianism and narcissism did not explain variation in trolling motivation. Moreover, being socially excluded had generally no effect on immediate trolling motivation, apart from participants with higher immediate trolling motivation, for whom the experience of social exclusion actually reduced trolling motivation. We show that not all facets of the Dark Tetrad are of equal importance for predicting immediate trolling motivation and that research should perhaps focus more on psychopathy and sadism. Moreover, our results emphasize the relevance of quantile regression in personality research and suggest that even psychopathy and sadism may not be suitable predictors for low levels of trolling behavior.