A surface was developed for surface-enhanced resonance Raman spectroscopy in the near-ultraviolet range. For this purpose, silver nanoparticles were synthesized, exhibiting localized surface plasmon resonance at an excitation wavelength of 410 nm. The nanoparticles were adsorbed onto the plane of a silver rod where the number of particles per unit area was controlled by the adsorption time. SERR spectra of cytochrome c were used as a reference system to quantify the enhancement factor of the newly developed surface. Optimal enhancement was obtained when the individual nanoparticle coverage on the surface reached a maximum. Nanoparticles touching each other in pairs or clusters led to shifts in the resonance frequency of resonantly coupled localized surface plasmons. Clusters therefore did not contribute to the enhancement factor at the wavelength of 413 nm. The overall surface enhancement factor finally achieved at this particular wavelength was in the range of 104−105.