Self-assembly Of Nanosheets Research Articles

Liquid Phase Exfoliation of Protein Parent Crystals into Nanosheets and Fibrils Based on Orthogonal Supramolecular Interactions.

Proteins are attractive building blocks for fabricating diverse and precise nanomaterials. However, the facile fabrication of multidimensional artificial assemblies is highly challenging. Here, inspired by the large-scale production technique of inorganic nanomaterials, we demonstrate the application of liquid phase exfoliation (LPE) on native protein ConA by the design of synthetic ligands. These ligands provide distinct in-plane and out-of-plane supramolecular interactions, allowing the generation of multidimensional architectures based on the same protein by dissociating a single interaction in solution, including 3D porous protein crystals, 2D sizable nanosheets, and 1D fibrils. Importantly, the exfoliated 2D sheets were dozens of times larger than the self-assembled nanosheets, resulting in a dramatic enhancement of the intrinsic bioactivity of the building blocks by receptor clustering and less endocytosis. These findings enable the successful application of LPE on biomacromolecules and open up an alternative avenue to generate advanced multidimensional nanomaterials, without the need for complex protein design and careful adjustment of self-assembly conditions.

Engineered self-assembled protein nanosheets for in situ biosynthesis of peptide anchored protein-semiconductor hybrids for efficient hydrogen production

Catalytic hydrogen production using solar energy is of great significance in addressing the global energy crisis. Herein, an efficient artificial photocatalytic hydrogen production system based on hierarchical protein self-assembly is designed and developed by in situ biosynthesis of peptide-anchored protein-semiconductor hybrids. The self-grown protein-CdS quantum dot hybrids stabilize and array orderly by the predesigned bioengineered proteins assembled into monolayer nanosheets, perfectly mimicking both the structure and function of the natural photosynthetic system. By mild deposition of Pt nanoparticles, the photocatalytic hydrogen production performance of the system is further enhanced to 69100 μmolh−1 (Cd2+) g −1, 80 times the catalytic activity of free CdS. Remarkably, the protein 2D network effectively enhances the water solubility, dispersibility, and solution stability of the inorganic catalytic centers, affording efficient photocatalytic hydrogen evolution. The system maintains 91.2 % catalytic efficiency after 30 days. Furthermore, its hydrogen production rate can be artificially regulated between 69100 and 52100 μmolh−1 (per g Cd2+) by the assembly-disassembly process of the protein templates. Thus, our work showcases the importance of the protein template and its assembly in maintaining the structural stability and high catalytic performance of the photocatalytic system and provides promising ideas for the simulation of natural photosynthetic systems.

Self-Assembled Blossom-Shaped NiCo2S4 Nanosheets In Situ Deposited Electrodes: Possessing High Reactivity and Selectivity for Bromine-Based Flow Batteries.

Bromine-based flow batteries (Br-FBs) are emerging rapidly due to their high energy density and wide potential window for renewable energy storage systems. Nevertheless, the sluggish kinetics of the Br2/Br- reaction on the electrode is considered to be the main challenge contributing to the poor performance of Br-FBs. Herein, we report self-assembled blossom-shaped NiCo2S4 nanosheets, enabling in situ growth on graphite felt (GF) via a one-step hydrothermal method. In the prepared NiCo2S4-GF, the gaps formed by the nanosheets restrict bromine diffusion, the sulfuretted blossom-shaped structure provides active sites with bromine adsorption capacity, and the synergistic effect of Ni and Co accelerates the electron transfer rate, which allow the electrode to exhibit excellent electrocatalytic activity compared to commercial GF, CoS-GF, and NiS-GF. Moreover, NiCo2S4-GF demonstrates unique selectivity for enhancing the Br2/Br- redox reaction compared to the bimetallic oxide of NiCo2O4-GF. Consequently, the zinc-bromine flow battery (ZBFB) with NiCo2S4-GF achieves an energy efficiency of 80.16%, which is 16.18% higher than that of the battery with commercialized GF at a current density of 60 mA cm-2, as well as a maximum power density of 260.75 mW cm-2 at 280 mA cm-2. The effective enhancement of the performance of ZBFB suggests that NiCo2S4-GF possesses great application potential in Br-FBs.

A Silver Modified Nanosheet Self-Assembled Hollow Microsphere with Enhanced Conductivity and Permeability.

The utilization of sheet structure composites as a viable conductive filler has been implemented in polymer-based electromagnetic shielding materials. However, the development of an innovative sheet structure to enhance electromagnetic shielding performance remains a significant challenge. Herein, we propose a novel design incorporating silver-modified nanosheet self-assembled hollow spheres to optimize their performance. The unique microporous structure of the hollow composite, combined with the self-assembled surface nanosheets, facilitates multiple reflections of electromagnetic waves, thereby enhancing the dissipation of electromagnetic energy. The contribution of absorbing and reflecting electromagnetic waves in hollow nanostructures could be attributed to both the inner and outer surfaces. When multiple reflection attenuation is implemented, the self-assembled stack structure of nanosheets outside the composite material significantly enhances the occurrence of multiple reflections, thereby effectively improving its shielding performance. The structure also facilitates multiple reflections of incoming electromagnetic waves at the internal and external interfaces of the material, thereby enhancing the shielding efficiency. Simultaneously, the incorporation of silver particles can enhance conductivity and further augment the shielding properties. Finally, the optimized Ag/NiSi-Ni nanocomposites can demonstrate superior initial permeability (2.1 × 10-6 H m-1), saturation magnetization (13.2 emu g-1), and conductivity (1.2 × 10-3 Ω•m). This work could offer insights for structural design of conductive fillers with improved electromagnetic shielding performance.

Interfacial Electrochemistry of Catalyst-Coordinated Graphene Nanoribbons.

The immobilization of molecular electrocatalysts on conductive electrodes is an appealing strategy for enhancing their overall activity relative to those of analogous molecular compounds. In this study, we report on the interfacial electrochemistry of self-assembled two-dimensional nanosheets of graphene nanoribbons (GNR-2DNS) and analogs containing a Rh-based hydrogen evolution reaction (HER) catalyst (RhGNR-2DNS) immobilized on conductive electrodes. Proton-coupled electron transfer (PCET) taking place at N-centers of the nanoribbons was utilized as an indirect reporter of the interfacial electric fields experienced by the monolayer nanosheet located within the electric double layer. The experimental Pourbaix diagrams were compared with a theoretical model, which derives the experimental Pourbaix slopes as a function of parameter f, a fraction of the interfacial potential drop experienced by the redox-active group. Interestingly, our study revealed that GNR-2DNS was strongly coupled to glassy carbon electrodes (f = 1), while RhGNR-2DNS was not (f = 0.15). We further investigated the HER mechanism by RhGNR-2DNS using electrochemical and X-ray absorption spectroelectrochemical methods and compared it to homogeneous molecular model compounds. RhGNR-2DNS was found to be an active HER electrocatalyst over a broader set of aqueous pH conditions than its molecular analogs. We find that the improved HER performance in the immobilized catalyst arises due to two factors. First, redox-active bipyrimidine-based ligands were shown to dramatically alter the activity of Rh sites by increasing the electron density at the active Rh center and providing RhGNR-2DNS with improved catalysis. Second, catalyst immobilization was found to prevent catalyst aggregation that was found to occur for the molecular analog in the basic pH. Overall, this study provides valuable insights into the mechanism by which catalyst immobilization can affect the overall electrocatalytic performance.

Self-assembled copper herbal carbon dots nanosheets with powerful antibacterial properties: A robust cascade antioxidant nanozyme for scavenging reactive oxygen species

Oxidative stress is a growing concern, necessitating more effective antioxidants for complex environments. Herein, we developed a composite nanozyme, DVI-CDs-Cu, with exceptional antioxidant and antibacterial properties by utilizing DVI-CDs derived from Chinese herbs: Dandelion, Violet, and Isatis root, complexed with Cu2+ ions. The DVI-CDs with abundant surface functional groups exhibited excellent superoxide dismutase-like (SOD-like) activity while forming stable complexes with copper ions. The attached copper ions performed the peroxidase-like (POD-like) function and formed a cascade effect, greatly enhancing the antioxidant capacity of the DVI-CDs-Cu nanozyme and strengthening its antibacterial property. The nanozyme was able to comprehensively clear 90 % of reactive oxygen species (ROS) while killing approximately 98 % of bacteria. The potential application of the nanozyme as a ROS scavenger was demonstrated by exploring its application to cigarette filters, capable of eliminating approximately 80 % of ROS. This provides a new case for the design and development of CDs-metal ions synergistic bionano-enzymes.

Gold Nanoparticles-Modified 2D Self-Assembled Amphiphilic Peptide Nanosheets with High Biocompatibility and Photothermal Therapy Efficiency.

Amphiphilic peptides have garnered significant attention due to their highly designable and self-assembling behaviors. Self-assembled peptides hold excellent potential in various fields such as biosensing, environmental monitoring, and drug delivery, owing to their remarkable biological, physical, and chemical properties. While nanomaterials formed by peptide self-assembly have found widespread use in biomedical applications, the development of 2D peptide nanosheets based on the self-assembly of amphiphilic peptides remains challenging in terms of rational design and morphology modulation. In this study, rationally designed amphiphilic peptide molecules are self-assembled into peptide nanosheets (PNS) under specific conditions to encapsulate gold nanoparticles (AuNPs), resulting in the formation of AuNPs/PNS hybrid materials with high photothermal conversion efficiency. The findings demonstrate that 2D PNS enhances the overall photothermal therapy effect of the nanohybrid materials due to their larger hosting area for AuNPs and higher biocompatibility. The well-designed amphiphilic peptides in this study offer insights into the structural design and functional modulation of self-assembled molecules. In addition, the constructed biomimetic-functional 2D inorganic/organic nanohybrid materials hold potential applications in biomedical engineering.

Self-assembled flower-like carbon nanosheets for magnetic solid-phase extraction of microcystins from aquatic organism

Adsorbents with good dispersibility and high efficiency are crucial for magnetic solid-phase extraction (MSPE). In this study, flower-like magnetic nanomaterials (F-Ni@NiO@ZnO2-C) were successfully prepared by calcination of metal-organic framework (MOF) precursors that was stacked by two-dimensional (2D) nanosheet. The synthesized F-Ni@NiO@ZnO2-C has a flower-like layered structure with a large amount of pore space, promoting the rapid diffusion of targets. In addition, Zn2+ doped in MOF precursors was still retained that further produced strong metal chelation with targets. The unique structure of F-Ni@NiO@ZnO2-C was used as MSPE adsorbent, and combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for extraction of three microcystins (MCs) detection, including microcystin-LR (MC-LR), microcystin-RR (MC-RR), microcystin-YR (MC-YR). The resulting method has a detection limit of 0.2–1.0 pg mL−1, a linear dynamic range of 0.6–500.0 pg mL−1 and has good linearity (R ≥ 0.9996). Finally, the established method was applied to the highly selective enrichment of MCs in biological samples, successfully detecting trace amounts of MCs (8.4–15.0 pg mL−1) with satisfactory recovery rates (83.7–103.1 %). The results indicated that flower-like magnetic F-Ni@NiO@ZnO2-C was a promising adsorbent, providing great potential for the determination of trace amounts of MCs in biological samples.

Unleashing the potential of self-assembled binder-free NiCoLDH nanosheets for supercapacitor

Unleashing the potential of self-assembled binder-free NiCoLDH nanosheets for supercapacitor

Multi-Responsive Peptide-Based Ultrathin Nanosheets Prepared by a Horizontal Monolayer Assembly.

In this study, peptide-based self-assembled nanosheets with a thickness of approximately 1 nm were prepared using a hierarchical covalent physical fabrication strategy. The covalent alternating polymerization of helical peptide E3 with an azobenzene (AZO) structure yielded copolymers CoP(E3-AZO), which physically self-assembled into ultrathin nanosheets in an unanticipated two-dimensional horizontal monolayer arrangement. This special monolayer arrangement enabled the thickness of the nanosheets to be equal to the cross-sectional diameter of a single linear copolymer, which is a rare phenomenon. Molecular dynamics simulations suggested that the synergistic effect of multiple molecular interactions drives the self-assembly of CoP(E3-AZO) into nanosheets and that various methods, including phototreatment, pH adjustment, the addition of additives, and introduction of cosolvents, can alter the molecular interactions and modulate the self-assembly of CoP(E3-AZO), yielding diverse nanostructures. Remarkably, the ultrathin nanosheets selectively inhibited cancer cells at certain concentrations.

Hydrophobic and zincophilic organic hierarchical nano-membranes with ordered molecular packing for stable zinc metal anodes

Rechargeable aqueous Zn metal batteries hold significant potentials as one of the next-generation energy-storage devices due to their low cost and high safety. However, the uncontrollable dendrite growth and low efficiency in plating/stripping of Zn anodes hinder the commercial application of aqueous Zn-ion batteries (AZIBs). Herein, inspired by hydrophobic lotus, a multifunctional organic hierarchical nano-membrane serving as an artificial protective layer is constructed through the gas-liquid interface method, using self-assembled organic nanosheets (NOS). The organic nano-membrane exhibits ordered molecular packing, accompanied by uniform distribution of zincophilic carbonyl (CO) groups on the Zn metal surface. These groups function as active sites, homogenizing Zn2+ flux and accelerating Zn2+ deposition. Simultaneously, the hydrophobic membrane isolates active water, thereby restraining side reactions, effectively suppressing corrosion and extremely inhibiting dendrite growth. Consequently, NOS@Zn anode exhibits stable cycling over 2400 h and an average coulombic efficiency (CE) of 99.69 % (600 cycles) at 1 mA cm−2. Furthermore, NOS@Zn||CNT-MnO2 full cell presents remarkable capacity retention, approximately 5 times higher than that of Zn||CNT-MnO2 after 3000 cycles at 2 A g−1. This work develops an innovative organic interface modification material paving the way for dendrite-free Zn metal anodes and advancing the practical application of large-scale Zn-based energy storage systems.

The nano-sheet structure adjustment and long-term stability of Zn-doped NiO electrochromic films

NiO is a typical anode electrochromic material based on its excellent electrochemical performance. However excellent cycle stability has always been the most important goal pursued by researchers. This study successfully prepared the complex multi-channel self-assembled nanosheet structure of low concentration and different Zn content doped NiO films with outstanding cycle stability on FTO by the low-temperature hydrothermal method. This excellent cycle stability provides a basis for applying NiO as a complementary layer in electrochromic devices. Zn doping influences the surface microstructure and electrochromic properties of NiO films. It is worth noting that all samples show large modulation amplitude, high coloring efficiency, and fast response time at the wavelength of 550 nm. The samples doped with 1% Zn exhibited more outstanding electrochromic properties than the samples without Zn doping, including fast switching speed (6.4 s and 3.8 s), significantly high coloration efficiency (52.49 cm2·C−1), transmittance modulation (68.57 % at 550 nm), and especially outstanding cycle stability (99.47 % after 5000 cycles). Zn doping affects the microstructure of the film surface, which greatly improves the cycle stability of the film by combining doping and structure control. We consider that the 1 % Zn-doped sample has the best cyclic stability because of the lower interface barrier between the 1 % Zn-doped sample and the FTO, the Zn-doped film forms a complex multi-channel structure, and the coupling effect makes the sample more conducive to the charge entry and exit.

Injectable Chitosan Hydrogels Doped with 2D Peptide Nanosheet-Drug Conjugates for Glutathione-Responsive Sustained Drug Delivery.

The development of novel and effective drug delivery systems aimed at enhancing therapeutic profile and efficacy of therapeutic agents is a critical challenge in modern medicine. This study presents an intelligent drug delivery system based on self-assembled two-dimensional peptide nanosheets (2D PNSs). Leveraging the tunable properties of amino acid structures and sequences, we design a peptide with the sequence of Fmoc-FKKGSHC, which self-assembles into 2D PNSs with uniform structure, high biocompatibility, and excellent degradability. Covalent attachment of thiol-modified doxorubicin (DOX) drugs to 2D PNSs via disulfide bond results in the peptide-drug conjugates (PDCs), which is denoted as PNS-SS-DOX. Subsequently, the PDCs are encapsulated within the injectable, thermosensitive chitosan (CS) hydrogels for drug delivery. The designed drug delivery system demonstrates outstanding pH-responsiveness and sustained drug release capabilities, which are facilitated by the characteristics of the CS hydrogels. Meanwhile, the covalently linked disulfide bond within the PNS-SS-DOX is responsive to intracellular glutathione (GSH) within tumor cells, enabling controlled drug release and significantly inhibiting the cancer cell growth. This responsive peptide-drug conjugate based on a 2D peptide nanoplatform paves the way for the development of smart drug delivery systems and has bright prospects in the future biomedicine field.

In situ assembly FeS2-tetrathiomolybdate nanosheets for imaging-guided tumor vascular collapse and amplified catalytic therapy

Catalytic therapy has recently gained considerable attention as a novel tumor microenvironment (TME)-responsive treatment. While conventional nano-catalysts have demonstrated the ability to induce the death of tumor cells through oxidative damage via the generation of reactive oxygen species (ROS), their therapeutic efficacy is impeded by several factors, including limited tissue penetration depth and suboptimal catalytic efficiency. Herein, the FeS2-tetrathiomolybdate (FeS2-TTM) nanosheet was synthesized via in-situ self-assembled in the specific redox TME using metal precursors of divalent iron and tetrathiomolybdate, and these metal precursors could easily enter tumor cells through vascular penetration to avoid the blood-brain barrier. This nanosheet has been proved to possess peroxidase (POD)-like catalytic activity, which overcomes the limitations of traditional nanoparticals by the bimetallic electron transfer effect in the TME to amplify the catalytic treatment effect. Importantly, it retains the chelating copper characteristics of TTM, and inhibits tumor angiogenesis by depriving the necessary copper in cells and angiogenesis, resulting in vascular embolization to cause tumor blood vessels to collapse. Additionally, it can also act as a nanoprobe that supports both real-time fluorescence imaging monitoring and T1/T2 dual-modality magnetic resonance imaging (MRI). As a promising nanomaterial, this new type of in-situ self-assembled nanosheet provides more varieties for the construction of an integrative platform of tumor diagnosis and treatment.

Theoretical and experimental study of flower-like NiMoS/NiO/NF with interface layer as a novel highly efficient bifunctional-electrode toward supercapacitor and HER

Electrocatalytic water splitting and supercapacitor systems are considered one of the most imperative sustainable energy storage and conversion technologies as an alternative clean energy carrier/source to fossil fuels in order to address the pressing global warming problems. For achieving sustainable electrochemistry devices, highly efficient and superior durability electrocatalysts must be prepared through a facile and controllable approach. This study successfully demonstrated the development of a binder-free 3D- flower-like amorphous structure composite with self-assembled nanosheets supported on Nickel foam (NiMoS/NiO/NF) by a simple two-step hydrothermal technique as advanced electrocatalysts for alkaline hydrogen evolution reaction (HER) and supercapacitor. The as-obtained NiMoS/NiO/NF only needs a overpotential of 43 mV vs. RHE to reach 10 mA.cm−2 and remained stable after long-term HER tests. In the following, the nanostructure was operated as an electrode in the supercapacitor. According to the Galvanostatic Charge-Discharge (GCD) teats, the specific capacitance of NiMoS/NiO/NF was measured as 999F/g. Such outstanding NiMoS/NiO/NF performance can be ascribed to the synthesis of the interface layer (NiO), which makes the robust adhesion between NF and the final layer (NiMoS). The amorphous morphology with multi-component doping and heterojunctions, optimizes the hydrogen desorption sites, which leads to significant HER catalytic activity. The present experimental results and density functional theory (DFT) study offer a cost-effective, and convenient pathway to produce highly efficient non-nobel-based metal as high-valued multifunctional materials.

Charge-Localized Retention and Long-Term Memory Enabled by Cooperating Sterically Confined Molecular Crystallization with Spiro[fluorene-9,9'-xanthene]-Based Csp3-Hindrance.

Charge localization of memory materials plays a crucial role in the endurance and retention ability of organic nonvolatile memory, which is completely opposite from the charge delocalization of high-mobility materials. However, charge transfer of both though-space and through-bond based on molecular design principles still faces challenges. Herein, a nonplanar wide-bandgap semiconductor with Csp3-hindrance (DOCH3-DDPA-SFX) has been designed and synthesized. An effective crystallization effect of self-assembled two-dimensional nanosheets on charge trapping dynamics and kinetics is visualized by Kelvin probe force microscopy (KPFM). The trapped charges are localized completely on a single nanosheet, which has better charge trapping and retention properties than an amorphous film. Meanwhile, crystallization also greatly improves structure stability. Combining DFT theoretical calculations, the mechanisms of localization and long-term retention are discussed. The steric crystallization effects on the charge localization will guide the effective design of single-component semiconducting charge-memory materials by molecular assembly and aggregate control for high-performance organic memory.

Dual Design on Hierarchically Hollow MoTe2/C with Ion/Electron Channel Engineering for High-Performance Sodium Storage.

Transition-metal tellurides have been investigated as novel anode materials for application in sodium-ion batteries (SIBs) due to their rich active sites and unique and controllable layered nanostructures. However, the weak structural strength and inferior intercalation/deintercalation kinetics inhibit the development of transition-metal tellurides. In this work, MoTe2/C composites with two different hollow nanostructures are designed and prepared. By adjustment of the precursor structure, MoTe2/C-2 exhibits superior sodium-storage performance because of its uniquely hollow nanostructure with self-assembled 2D flexible nanosheets grown on the external surface. MoTe2/C-2 delivers a higher specific capacity (276 mAh g-1 at 0.1 A g-1 after 300 cycles), much more than MoTe2/C-1 (201 mAh g-1 at 0.1 A g-1 after 300 cycles), and exhibits a long-time cycling performance (131 mAh g-1 at 1 A g-1 after 2000 cycles). The excellent sodium-storage performance derived from the rational structure design is beneficial for shortening the ion paths, facilitating the sodiation/desodiation process, and reinforcing the intrinsic structural stability, thus boosting the reaction kinetics and prolonging the cycling life. Meanwhile, the assembled full-cell maintains 101 mAh g-1 at 0.1 A g-1 after 50 cycles and lights an electric watch. The findings provide several new views for preparation of more transition-metal tellurides with multi-ion/electron migration channel engineering.

Endoplasmic Reticulum-Targeting Self-Assembly Nanosheets Promote Autophagy and Regulate Immunosuppressive Tumor Microenvironment for Efficient Photodynamic Immunotherapy.

The poor efficiency and low immunogenicity of photodynamic therapy (PDT), and the immunosuppressive tumor microenvironment (ITM) lead to tumor recurrence and metastasis. In this work, TCPP-TER-Zn@RSV nanosheets (TZR NSs) that co-assembled from the endoplasmic reticulum (ER)-targeting photosensitizer TCPP-TER-Zn nanosheets (TZ NSs for short) and the autophagy promoting and indoleamine-(2, 3)-dioxygenase (IDO) inhibitor-like resveratrol (RSV) are fabricated to enhance antitumor PDT. TZR NSs exhibit improved therapeutic efficiency and amplified immunogenic cancer cell death (ICD) by ER targeting PDT and ER autophagy promotion. TZR NSs reversed the ITM with an increase of CD8+ T cells and reduce of immunosuppressive Foxp3 regulatory T cells, which effectively burst antitumor immunity thus clearing residual tumor cells. The ER-targeting TZR NSs developed in this paper presents a simple but valuable reference for high-efficiency tumor photodynamic immunotherapy.

Relationship between molecular structure and corrugations in self-assembled polypeptoid nanosheets revealed by cryogenic electron microscopy

Designing conformationally dynamic molecules that self-assemble into predictable nanostructures remains an important unmet challenge. This paper describes how atomic-scale cryogenic transmission electron microscopy (cryo-TEM) can be used to explore the relationship between molecular structure and self-assembly of block copolymers. We examined sheetlike micelles formed in water using a series of diblock copolypeptoids with the same hydrophilic block and three distinct crystalline hydrophobic blocks. Our cryo-TEM images revealed all the structures share nansoscale features, but differ in their intermolecular packing geometries. Different molecular arrangements, parallel and antiparallel V-shaped crystal motifs, were revealed by two-dimensional atomic-scale through-plane images. However, images from tilted samples revealed an unexpected feature when the hydrophobic polypeptoid block comprised phenyl rings with substituted bromine atoms at the para position. The nanosheets contained atomic-scale corrugations that were absent in the other systems which comprised unsubstituted aliphatic and aromatic side chains. We hypothesize that these corrugations are due to the dipolar characteristics of the brominated phenyl group and interactions between this group and water molecules. Published by the American Physical Society 2024

Synergistically self-assembled 2D nanosheets of MXene@MOF derived CoW-LDH into 3D frameworks functionalized with chitosan for improved skin wound healing

Nature-inspired multifunctional composites are suitable materials for tissue reconstruction. Treating bacterial infections and promoting wound healing/skin regeneration remain major concerns in clinical practise and basic research. Single-modal treatment tends to be unreliable. In this study, multipurpose CS/MXene@LDH hybrid nanocomposites (NCs) are manufactured using chitosan functionalized with self-assembled negatively -charged MXene and positively -charged cobalt tungsten (CoW) layered doubled hydroxide (LDH) to develop promising wound-dressing materials. The physicochemical characteristics of hybrid NCs were investigated using XRD, XPS, FTIR, SEM, and TEM. The in-vitro results reveal that the CS/MXene@LDH hybrid NCs improve wound healing behaviour and acceptable cytocompatibility. Furthermore, in-vivo studies show that the hybrid NCs successfully inhibits the inflammatory response and promote fibroblast growth, granulation tissue formation, deposition of collagen, and re-epithelialisation of skin wounds, resulting in wound healing in mice. These findings indicate that the CS/MXene@LDH hybrid NCs is good candidates for wound-healing applications.