A method has been developed for labelling PK 14105 [ N-methyl- N-(1-methyl-propyl)-1(2-fluoro-5-nitro- phenyl)isoquinoline-3-carboxamide], a ligand that has high affinity and selectivity for peripheral type benzodiazepine binding sites (PBBS), with NCA fluorine-18 ( t 1 2 = 109.8 min, β + = 96.9%). The method involves treating the 2-chloro-analogue with cyclotron-produced NCA [ 18F]fluoride in dimethyl sulphoxide, with rubidium carbonate as base, at 140°C for 20 min. Purification is achieved by separation on a reverse phase Sep-Pak followed by HPLC on a silica gel column, to give chemically and radiochemically pure product with a specific activity of ca 7.4 GBq/μmol (200 mCi/μmol), decay-corrected to the end of radionuclide production (EOB). The radiosynthesis requires 210 min, giving a radiochemical yield of 10–20%, decay-corrected to EOB. [ 18F]PK 14105 was found to bind avidly to sites associated with kainic acid-induced unilateral lesions of rat striata. Such binding was blocked by pre-dosing the rat with PK 11195, so providing evidence for specific binding to PBBS. These results suggest that [ 18F]PK 14105 has potential for studying phenomena associated with PBBS in man by PET.