In this work, new membrane was fabricated from polyester fabric (PF) as a substrate which was coated by a casting solution containing polyvinylidene fluoride (PVDF) blending by addition of MIL-101 (Cr) metal-organic framework (MOF)-molecularly imprinted 3-aminophenolhexamethylenetetramine (MIAPH) as filler and sodium dodecyl sulfate (SDS) as hydrophilic agent. The membrane was fabricated by phase inversion method (PVDF/MIAPH). The MIAPH has selective functional groups and was applied as filler in the mixed matrix membrane. The as-prepared MIAPH was characterized by FE-SEM, EDS, XRD, FTIR and BET-BJH analyses. The hydrophilic property, dye adsorption and filtration properties of the MIAPH/PVDF/PF membrane were studied for removal Trypan blue (TB) based on central composite design (CCD) technique through investigating operational variables (pH, TB concentration, pressure). In the fabrication of membranes, the optimal filtration properties appear at the 30μL of SDS solution and 0.005g MIAPH fillers. The fabricated MIAPH/PVDF/PF membrane indicates a high removal efficiency up to 94.9% for the TB removal and has high selectivity. The data of the adsorption equilibrium were described by the Langmuir, Freundlich, Temkin and Dubinin-Radushkevich isotherms. The findings illustrated that appropriate of MIAPH in membranes could be an efficient and selective technique for TB elimination.