The cue rate (CR: blows per whale per hour), surfacing characteristics and swim speeds of sei whales (Balaenoptera borealis) were quantified fromfocal follows carried out at Berkeley Sound (East Falkland) between January and May 2017 and off the west coast of West Falkland betweenFebruary and April 2018. In Berkeley Sound, focal follows were conducted from Cape Pembroke lighthouse and from a small boat. In West Falklandall focal follows were conducted from a yacht. Thirty-seven focal follows of sei whale individuals or groups (2–5 individuals) were analysed toproduce CRs ranging from 21.99 to 46.73, with a mean of 31.46 (SD = 5.12). There was no significant difference in the CRs observed from shorevs. boat platforms or between the two study areas. Maximum submergence times exceeding 13min were recorded from both individuals and groups.The durations of 51 whale surfacing events had a mean of 6.4s (SD = 1.7). The average swim speed during boat-based sei whale focal follows was5.7kmh−1. The inter-breath intervals (IBIs) recorded from 13 solitary individuals ranged from 77.2 to 180.1s, with an overall mean of 118.6s (SD= 137.6). A combined approach incorporating IBI parameters and sequence pattern was used to classify 270 IBIs into surface dives (mean = 37.2s),intermediate dives (mean = 113.7s) and true dives (mean = 332.6s). Individuals showed marked variation in dive pattern, with some exhibitingclear cycles of true dives interspersed with surface bouts while others routinely took intermediate-duration dives interspersed by single surfacings.Sei whales in Berkeley Sound exhibited a higher proportion of surface dives than whales in West Falkland, and those surface dives were of lowermean and median IBI. Individual sei whales had surface bouts comprising a mean of 3.8 blows and a mean IBI of 33.4s. These are the firstquantifiable data on surfacing-dive patterns and CRs for sei whales in the Falkland Islands and across the wider range of the species. The data haveconservation and management relevance, including addressing availability bias for line transect and cue count abundance estimates, incorporationinto vessel strike modelling, and understanding foraging behaviour.