Abstract Anomalous influxes of water into unfilled collection bags can greatly overestimate volume and flow rate data from seepage meters. From static tank trials, initially empty collection bags (4,500 mL capacity) attached to seepage meters gained significantly more water relative to bags prefilled to 1,000 mL. Data from a study of groundwater flow at redds of brook trout Salvelinus fontinalis in Scott Lake, Ontario, indicate that the use of unfilled bags biases seepage meter data. At these redds, the anomalous influx of water into unfilled bags was significant (intercept of regression equation, y = 275 mL); however, this influx was sufficiently reduced when prefilled bags were used (v = 34 mL). Our data suggest that even at high flow rates (22–169 mL·m−2·min−1), seepage measures can be inflated by an order of magnitude when initially empty bags are used. Because of this anomaly, previous measures of groundwater flow at brook trout redds with unfilled bags are probably not representative of natural flow...