Pea Seeds Research Articles

Oxidative homeostasis in germinating pea seeds (isum sativum) depending on ultrasonic exposure duration

The effects of ultrasonic exposure durations (5, 10 and 20 min) with intensity of 25 kW/m2 and the frequency of 26.1 kHz on the extent of lipid peroxidation, oxidative modification of proteins, the activity of cysteine proteinases, and gene expression in germinating pea seeds were studied. It has been shown that the intensity in the area that had seeds planted (just over the central ultrasound generator) was rather uniformly distributed, but at the same time gave rise to diffuse reflection. A range of tendencies in changes of the indicators under study were seen after ultrasound exposure. The level of malonic dialdehyde increased with increasing duration of ultrasound exposure. This study revealed the increased amount of oxidized proteins in germinating pea seeds after a 10 min-exposure to ultrasound, and a decrease in the level of oxidative modification of proteins present in seeds subjected to ultrasound for a period of 20 min. The activity of cysteine proteinases was higher in pea seeds after a 5-min exposure to ultrasound, but the amount of mRNA transcripts increased in all experimental samples.

Novel Milk Substitute Based on Pea, Bean and Sunflower Seeds with Natural Bioactive Stabilisers.

The aim of this research was to create a plant-based beverage based on seeds of sunflower (Helianthus annuus), pea (Pisum sativum) and runner bean (Phaseolus multiflorus). The selection of the ingredients was based on the main objective to obtain the nutritional value and sensory characteristics of a formed product similar to cow's milk. The ingredient proportions were created by comparing the protein, fat and carbohydrate content of seeds versus cow's milk. Due to the observed low long-term stability of plant-seed-based drinks, a water binding guar gum, a thickener in the form of locust bean gum and gelling citrus amidated pectin containing dextrose were added and evaluated as functional stabilisers. All of the designed and created systems were subjected to selected methods of characterisation of the most important final product properties, such as rheology, colour, emulsion and turbidimetric stability. Rheological analysis confirmed the highest stability of the variant supplemented with 0.5% guar gum. Both stability and colour measurements indicated the positive characteristics of the system supplemented with 0.4% pectin. Finally, the product with 0.5% guar gum was identified as the most distinctive and similar vegetable drink to cow's milk.

Analysis of nucleotide sequences of the ABI3 gene coding region in pea varieties with different percentage of seed storage proteins

The main source of vegetable protein in the world are leguminous crops, among which peas are the most common in Russia. Dozens and even hundreds of genes are involved in the regulation of biological processes associated with the maturation of seeds and the accumulation of reserve nutrients in them, including proteins. The study conducted on legume species related to peas indicate that one of the genes at the highest hierarchical level in this regulatory chain is the ABI3 transcription factor gene; however, its role in the biosynthesis and accumulation of seed storage proteins remains poorly studied. The purpose of the current work is to identify high-protein pea genotypes and analyze the nucleotide sequences of the coding region of the ABI3 gene to search for possible DNA polymorphisms associated with protein percentage in their seeds. The study was carried out in 2020–2021. The objects of the study were 37 pea varieties from the collection of genetic resources of leguminous crops of VIR. Protein percentage in seeds was determined by the Bradford method. Selection of primers and comparative analysis of nucleotide sequences were performed using the PrimerSelect and MegAlign programs. Sequencing was performed according to the Sanger method. There were identified pea varieties with the highest (‘Aksaisky Usaty 55’, ‘Sakharny’) and low (‘K-8361’ (VIR), ‘Fregat’) seed protein percentage. In these variety samples, the coding region of the ABI3 gene was sequenced. Comparative analysis of their nucleotide sequences with the sequence of the ABI3 gene fragment annotated in the GenBank database revealed significant variability among them: nucleotide substitutions, deletion of 6 nucleotides. In the high protein variety ‘Sakharny’, there has been found a 9-nucleotide insertion, leading to the insertion of three additional amino acids, and in the low-protein variety Fregat, there has been found a single nucleotide insertion, causing a shift in the reading frame. The data obtained may indicate a correlation between mutations in the ABI3 gene and protein accumulation in pea seeds.

PHYSICAL AND CHEMICAL SCARIFICATION ON DORMANCY BREAKING OF ROSARY PEA SEEDS (ABRUS PRECATORIUS L.)


 
 
 
 Abstract: Seeds are part of the plant containing embryo that grows through the germination process. This process involves metabolism occurs in seeds to produce roots and shoots. However, this metabolic process is inhibited by the environment and genetics of the seeds itself. Thus, the seeds experience dormancy and fail to grow. Dormancy can be broken with help of physical and chemical scarification to accelerate seed germination. The research aim was to determine the physical and chemical scarification seed dormancy breaking in increasing the germination rosary pea seeds (Abrus precatorius L.). The research method used was experimental randomized block design using 4 treatment groups, namely cracked (F1), sanded (F2), and soaked in 50% H2SO4 solution for 15 minutes (K1) and 30 minutes (K2) and the group control with water. The viability germination, germination rate (GER), vigor index (VI), simultaneous growth (SG) normal sprouts on day 6, and growth rate (GOR) were observed for two weeks. The results obtained that immersion in H2SO4 50% solution for 30 minutes and cracking could break the dormancy of rosary pea seeds and increase germination with higher viability of germination, GER, VI, SG, and GOR than immersion in 50% H2SO4 solution for 15 minutes and sanding.
 
 
 

White Lupin as a New Crop for Plant Proteins

The world is demanding more protein for human consumption-increasing amounts of plant proteins are being used to meet this increasing demand. It has been estimated that global plant-based alternative protein market could swell to $162 billion in the next decade from $29.4 billion in 2020 and every tenth portion of meat, eggs, and dairy eaten around the globe by 2035 could be derived from plant proteins. White lupin (Lupinus albus L.), a legume, has been researched in Virginia for several years which has resulted in development of several winter-hardy and high yielding lines. However, concentrations of protein and relative concentrations of various amino acids in seeds of these lines are not known. Therefore, objective of this study was to characterize protein in winter-hardy lupin lines. Seeds of five winter-hardy white lupin lines grown during 2020-2021 contained about 51% protein as compared to literature values of about 35 and 24% protein in soybean and winter pea seeds, respectively. Concentrations of nine essential amino acids (isoleucine, leucine, lysine, methionine, threonine, tryptophan, valine, phenylalanine, and histidine) in lupin seed varied from 1.25 to 1.41, 1.98 to 2.51, 1.12 to 1.60, 0.21 to 0.27, 1.03 to 1.28, 0.25 to 0.30, 1.22 to 1.40, 1.14 to 1.28, and 0.69 to 0.79, respectively. These concentrations compared quite well with those in soybean and winter pea seed. These results indicate that white lupin has considerable potential to meet alternative plant protein needs of manufactures and consumers.

EFFECTS OF SOAKING, BOILING, AND TOASTING PROCESSING TECHNIQUES ON NUTRIENT COMPOSITION OF PIGEON PEA FLOURS AND ORGANOLEPTIC ATTRIBUTES OF THEIR PUDDINGS AND BALLS.

This study evaluated the effects of soaking, boiling and toasting processing techniques on nutrient andphytochemical composition of pigeon pea flour and the organoleptic properties of their puddings and balls.Pigeon pea and cowpea seeds which served as control were purchased, processed into flour, used for thepreparation of puddings and balls. The pigeon pea flour samples were subjected to proximate, vitamin andmineral analysis using standard methods. The data generated from organoleptic evaluation, proximate,vitamins and minerals analyses respectively, were subjected to statistical analysis with descriptive statisticsSPSS version 20. One way ANOVA was used to compare mean at p<0.05 probability level. Findings showthat the processing techniques significantly improved the nutrient properties of the pigeon pea flours.However, toasted pigeon pea flour had the highest folate (vitamin B9) content of 644.530mcg/100g andphosphorus content. Boiled pigeon pea flour had the highest iron content of 2.205mcg/100g.. Boiled pigeonpea flour had the highest protein and iron content and soaked pigeon pea flour had the highest moisture,crude fiber and Vitamin B3 contents. Hence, to improve micronutrient intake such as folate and phosphorus,toasting processing technique is highly recommended

A Comparative Study on Different Methods of Inoculating Cowpea Using Microbial Fertilisers

This study was based on setting up a method of inoculating cow pea using microbial fertilizers. the study was designed to evaluate the effects of inoculating bio fertilizers made from rhizobium bacterial with cowpea seeds so as to propose strategies for sustainable management of it. After putting in place six experimental set up made up of mixtures of: cowpea and rhizobium alone, cowpea with rhizobium substrate, cowpea plus rhizobium and gray powder, cowpea with rhizobium and phosphor solubilizing microbes (PSM), with two control experiment of which one set up had no inoculants and the other had just gray powder. The following parameters were verified: the height of plants after two months, average number of nodules per treatment and identification of various diseases attack on the different treated seedlings. Primary data were subjected to analysis of variance (ANOVA). The following results were obtained: the set up made of cowpea with rhizobium and grey powder gave best results with 39.70 cm as average height, 48 as an average number of nodules on these treated plants, no diseases attack due to the fact that Rhizobium which is a bacterium is used alongside with another ingredient. Cowpea with rhizobium and PSM also gave interesting results with average nodules of 41; 36.11cm as average height of plants and no disease. Apart from cowpea treated just with rhizobium alone which also showed average results with 27cm as average height and 27 as average number of nodules with no disease incidence. The control experiments together with cowpea treated with no rhizobium showed negative results with various diseases attack such as angular leave spots disease on seeds treated with grey powder, damping off of seedling for seeds treated with rhizobium substrate, and yellow cercosporiose for seeds without treatment. As recommendation, it is advisable to inoculate cow pea seeds with other ingredients as grey powder before planting.

Combination of three null mutations affecting seed protein accumulation in pea (Pisum sativum L.) impacts positively on digestibility

The presence of so-called anti-nutritional factors can reduce the bioavailability of nutrients following consumption of seeds which are otherwise an excellent source of proteins, carbohydrates and micronutrients. Among the proteins associated with negative effects on quality in pea (Pisum sativum L.) seeds are lectin, pea albumin 2 (PA2) and trypsin inhibitors (TI). Here we have investigated the impact of these proteins on protein digestibility and amino acid availability, using naturally occurring and derived mutant lines of pea lacking these proteins. The mutations were stacked to generate a triple mutant which was compared with a wild-type progenitor and a line lacking the major seed trypsin inhibitors alone. In vitro digestions following the INFOGEST protocol revealed significant differences in the degree of hydrolysis, protein profile and apparent amino acid availability among the pea variants. Proteins resistant to digestion were identified by MALDI-TOF mass spectrometry and amino acid profiles of digested samples determined. The results indicate that pea seeds lacking certain proteins can be used in the development of novel foods which have improved protein digestibility, and without negative impact on seed protein concentration or yield.

Effect of infrared heating on the functional properties of yellow pea and green lentil flours

AbstractBackground and ObjectivesValue‐added utilization of pulse flours faces challenges related to their functionality in many food applications. The present research assessed the use of infrared heating (120°C vs. 140°C) tempered (20% vs. 30% moisture) green lentil and yellow pea seeds as a means of tailoring their functional properties.FindingsSome flour functionalities were mildly affected by processing and, in most cases, were correlated with protein surface hydrophobicity and damaged starch content. Solubility at pH 5 was relatively unchanged in response to processing, while the values were slightly lowered at pH 7. The water (WHC) and oil holding capacities (OHC) improved, although OHC tended to decline as heating temperatures increased. Both pulses had poor foaming capacities but high foaming stabilities that remained constant after processing. The highest emulsion activity (EA) for pea was with the 120°C and 30% moisture treatment whereas for lentil it was with 120°C and 20% moisture; the emulsion capacity declined after all treatments.ConclusionsSelect conditions of infrared heating coupled with tempering of pulse seeds before milling can modestly improve the flour's EA, WHC, or OHC.Significance and NoveltyYellow pea and green lentil flours from infrared pretreated seeds can now be more easily formulated into applications based on their functional properties.

Determination of Some Physical and Physiological Properties of Seeds of Different Grass Pea (Lathyrus sativus L.) Genotypes

Determination of Some Physical and Physiological Properties of Seeds of Different Grass Pea (Lathyrus sativus L.) Genotypes

Enhanced accumulation of phenolics in pea (Pisum sativum L.) seeds upon foliar application of selenate or zinc oxide.

Selenium (Se) and zinc (Zn) are essential antioxidant enzyme cofactors. Foliar Se/Zn application is a highly effective method of plant biofortification. However, little is known about the effect of such applications on the concentration of trace elements and phytochemicals with pro-oxidant or antioxidant activity in pea (Pisum sativum L.). A 2-year pot experiment (2014/2015) was conducted to examine the response of two pea varieties (Ambassador and Premium) to foliar-administered sodium selenate (0/50/100 g Se/ha) and zinc oxide (0/375/750 g Zn/ha) at the flowering stage. Concentrations of selected trace elements (Fe, Cu, and Mn), total phenolic content (TPC), total flavonoid content (TFC), and total antioxidant activity (ABTS, FRAP) of seeds were determined. Se/Zn treatments did not improve the concentration of trace elements, while they generally enhanced TPC. Among examined treatments, the highest TPC was found in Ambassador (from 2014) treated with 100 g Se/ha and 750 g Zn/ha (2,926 and 3,221 mg/100 g DW, respectively) vs. the control (1,737 mg/100 g DW). In addition, 50 g of Se/ha increased TFC vs. the control (261 vs. 151 mg/100 g DW) in Premium (from 2014), 750 g of Zn/ha increased ABTS vs. the control (25.2 vs. 59.5 mg/100 g DW) in Ambassador (from 2015), and 50 g of Se/ha increased FRAP vs. the control (26.6 vs. 18.0 mmol/100 g DW) in Ambassador (from 2015). In linear multivariable regression models, Zn, Mn, Cu, and TPC best explained ABTS (R = 0.577), while Se, Cu, and TPC best explained the FRAP findings (R = 0.696). This study highlights the potential of foliar biofortification with trace elements for producing pea/pea products rich in bioactive plant metabolites beneficial for human health.

Influence of germination on the bioactive compounds, antioxidant capacity and thermic characteristics of Phaseolus vulgaris L. and Cajanus Cajan seeds

Aim: To evaluate the effect of germination on total phenols, antioxidant capacity, trace elements, and thermal behavior of the seeds of Pigeon peas (Cajanus cajan L.) and Chacha and Recline varieties of the common bean (Phaseolus vulgarisL.). Methodology: The evaluation of the total phenols was done using Folin Ciocalteu’s spectrophotometric method; the antioxidant capacity through the DPPH y ABTSº+ radicals;the macro and microelements using the spectrophotometric optical emission coupled inductively with plasma method ICP-OES; and the thermic behavior through differential scanning calorimetry (DSC). Results: The germination process influenced the total polyphenol content, antioxidant capacity, and macro and microelements in each specie that was studied. For example, for the Recline variety seedlings, the phenol content increased by 57.75% in comparison to the seed. The antioxidant capacity against DPPH and ABTS º+ was greater in the seedlings in the following order: Chaucha variety>Pigeon pea>Recline variety. For the seeds, the macro and micro-element content had the following order: K>Mg>Ca>Na y Fe>Zn>Cu>Cr; for the seedlings, the greatest decrease was in K, Cr and the greatest increase was in Ca and Mn; the Na:K relationship was less than one. Finally, the thermic behavior, in reference to the gelatinization enthalpy (ΔH) was greater for the seed than the seedlings.

Validating Protocol and Deciphering the Nanoparticulate Seed Treatment in Enhancing Seed Quality of Soybean, Pigeon Pea and Groundnut

Silicon nanoparticles have attracted huge interest as a rapidly growing class of materials for many agricultural applications. It provided new insight of the potential growth promoting effects of the nanoparticle (SiO­2) on plant system. Looking in to its importance, a comprehensive study was conducted to standardize the protocol on method of seed treatment with Silicon dioxide (SiO2) nanoparticle for enhancing seed quality in soybean, groundnut and pigeon pea. Among the treatment combinations, soybean, groundnut and pigeon pea seeds treated with SiO2 nanoparticle in powder form along with polymer found to be superior for all the tested seed quality parameters viz., germination (95.00, 95.33 and 94.67%), seedling length (39.60, 35.14, 25.56cm), mean seedling dry weight (45.53, 28.33 and 50.47mg/seedling), seedling vigour index-I (3763, 3348 and 2421), seedling vigour index-II (4332, 2700 and 4780) for soybean, pigeon pea and groundnut, respectively. However, compared to untreated control, these seed quality attributes were statistically on par with CMC+NP treatment. These findings suggested that seed treatment with SiO2 nanoparticle in powder form along with polymer coating significantly enhanced seed quality of soybean, groundnut, and pigeon pea.

Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings

BackgroundSeaweeds are a viable bioresource for suffering plants against salt stress, as they abundant in nutrients, hormones, vitamins, secondary metabolites, and many other phytochemicals that sustain plants’ growth under both typical and stressful situations. The alleviating capacity of extracts from three brown algae (Sargassum vulgare, Colpomenia sinuosa, and Pandia pavonica) in pea (Pisum sativum L.) was investigated in this study. MethodsPea seeds were primed for 2 h either with seaweed extracts (SWEs) or distilled water. Seeds were then subjected to salinity levels of 0.0, 50, 100, and 150 mM NaCl. On the 21st day, seedlings were harvested for growth, physiological and molecular investigations. ResultsSWEs helped reduce the adverse effects of salinity on pea, with S. vulgare extract being the most effective. Furthermore, SWEs diminished the effect of NaCl-salinity on germination, growth rate, and pigment content and raised the osmolytes proline and glycine betaine levels. On the molecular level, two low-molecular-weight proteins were newly synthesized by the NaCl treatments and three by priming pea seeds with SWEs. The number of inter-simple sequence repeats (ISSR) markers increased from 20 in the control to 36 in 150 mM NaCl-treated seedlings, including four unique markers. Priming with SWEs triggered more markers than the control, however about ten of the salinity-induced markers were not detected following seed priming before NaCl treatments. By priming with SWEs, seven unique markers were elicited. ConclusionAll in all, priming with SWEs alleviated salinity stress on pea seedlings. Salinity-responsive proteins and ISSR markers are produced in response to salt stress and priming with SWEs.

A comprehensive and conceptual overview of omics-based approaches for enhancing the resilience of vegetable crops against abiotic stresses.

Abiotic stresses adversely affect the productivity and production of vegetable crops. The increasing number of crop genomes that have been sequenced or re-sequenced provides a set of computationally anticipated abiotic stress-related responsive genes on which further research may be focused. Knowledge of omics approaches and other advanced molecular tools have all been employed to understand the complex biology of these abiotic stresses. A vegetable can be defined as any component of a plant that is eaten for food. These plant parts may be celery stems, spinach leaves, radish roots, potato tubers, garlic bulbs, immature cauliflower flowers, cucumber fruits, and pea seeds. Abiotic stresses, such as deficient or excessive water, high temperature, cold, salinity, oxidative, heavy metals, and osmotic stress, are responsible for the adverse activity in plants and, ultimately major concern for decreasing yield in many vegetable crops. At the morphological level, altered leaf, shoot and root growth, altered life cycle duration and fewer or smaller organs can be observed. Likewise different physiological and biochemical/molecular processes are also affected in response to these abiotic stresses. In order to adapt and survive in a variety of stressful situations, plants have evolved physiological, biochemical, and molecular response mechanisms. A comprehensive understanding of the vegetable's response to different abiotic stresses and the identification of tolerant genotypes are essential to strengthening each vegetable's breeding program. The advances in genomics and next-generation sequencing have enabled the sequencing of many plant genomes over the last twenty years. A combination of modern genomics (MAS, GWAS, genomic selection, transgenic breeding, and gene editing), transcriptomics, and proteomics along with next-generation sequencing provides an array of new powerful approaches to the study of vegetable crops. This review examines the overall impact of major abiotic stresses on vegetables, adaptive mechanisms and functional genomic, transcriptomic, and proteomic processes used by researchers to minimize these challenges. The current status of genomics technologies for developing adaptable vegetable cultivars that will perform better in future climates is also examined.

Prediction of Pea (Pisum sativum L.) Seeds Yield Using Artificial Neural Networks

A sufficiently early and accurate prediction can help to steer crop yields more consciously, resulting in food security, especially with an expanding world population. Additionally, prediction related to the possibility of reducing agricultural chemistry is very important in an era of climate change. This study analyzes the performance of pea (Pisum sativum L.) seed yield prediction by a linear (MLR) and non-linear (ANN) model. The study used meteorological, agronomic and phytophysical data from 2016–2020. The neural model (N2) generated highly accurate predictions of pea seed yield—the correlation coefficient was 0.936, and the RMS and MAPE errors were 0.443 and 7.976, respectively. The model significantly outperformed the multiple linear regression model (RS2), which had an RMS error of 6.401 and an MAPE error of 148.585. The sensitivity analysis carried out for the neural network showed that the characteristics with the greatest influence on the yield of pea seeds were the date of onset of maturity, the date of harvest, the total amount of rainfall and the mean air temperature.

Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth.

Plant-growth promoting rhizobacteria (PGPR) play a vital role in soil fertility and crop production. The rhizosphere of many crop plants has been well documented by screening PGPR for their plant-growth promoting (PGP) mechanisms. However, the rhizosphere of grass species that may act as potential habitats for novel PGPR remains relatively unexplored. Ageratina adenophora is a noxious weed that has invaded more than 40 tropical and subtropical countries in Asia, Oceania, Africa, and Europe. Its presence has led to changes in plant species composition, reducing their biodiversity and destroying ecosystem function. In this study, we screened 1,200 bacterial strains isolated from the rhizosphere soil of A. adenophora in three floristic regions in Yunnan Province, China. Samples were screened for their in vitro ability for N-fixation, production of the plant growth regulator indole-3-acetic acid (IAA), and the synthesis of 1-amino-cyclopropane-1-carboxylate (ACC) deaminase, which controls the levels of ethylene in developing plant roots. We found that 144 strains showed at least one of these PGP attributes. 16S rRNA gene sequencing showed that most (62.5%) of the samples were bacteria closely related to members of the genera Pseudomonas (27 strains), Providencia (20 strains), Chryseobacterium (14 strains), Ensifer (12 strains), Enterobacter (nine strains), and Hafnia (eight strains). Their abundance and biodiversity in the soil of individual floristic regions correlate positively with the invasion history of A. adenophora. From these PGP bacterial strains, KM_A34 (Pantoea agglomerans), KM_C04 (Enterobacter asburiae), and KM_A57 (Pseudomonas putida), which had the greatest in vitro ability of N-fixation, and IAA and ACC deaminase production, respectively, were selected. The strains were evaluated for their effect on the seed germination and growth of soybean, faba bean, pea, wheat, and Chinese cabbage other than A. adenophora. Chamber experiments showed these strains significantly (P < 0.05) increased (14.2-43.4% over the controls) germination rates of the soybean, faba bean, pea, and/or Chinese cabbage seeds. They also reduced relative seed germination times (20.8-48.8% over the controls) of soy bean, faba bean and/or wheat seeds. Greenhouse pot experiments showed that they significantly (P < 0.05) promoted the aboveground and belowground height of plant foliage (12.1-23.1% and 11.5-31.4% over the controls, respectively) and/or the dry weights (16.1-33.5% and 10.6-23.4% over the controls, respectively) of the soy bean, faba bean, pea, wheat and/or Chinese cabbage. These data indicate that the rhizosphere microbiota of A. adenophora contain a PGPR pool that may be used as bioinoculants to improve the growth and productivity of these crops.

Response of selected nutrient elements in pea (Pisum sativum L.) leaf tissues to the seed priming-and-drying technology in cucurbitacin phytonematicides

Priming-and-drying technology for pea (Pisum sativum L.) seeds with hypogeal germination showed that the technology could improve plant growth and development, while suppressing nematode population densities, but without information on the potential causal factors for the former. A study was, therefore, conducted in Limpopo Province, South Africa, to investigate the extent to which plant responses could be associated with accumulation of foliar nutrient elements in pea plants post-subjecting pea seeds to the technology. After a 2-h priming of pea seeds in geometric concentration of Nemarioc-AL and Nemafric-BL phytonematicides and then air-dried in ovens at 26ºC for 72 h. Seeds were sown in 20-cm-diameter plastic pots filled with appropriate growing mixture, with one trial in the greenhouse and the other on microplots. At 60 days after sowing, 10 healthy mature leaves per plant were prepared for extraction of nutrient elements through the digestion method and then quantified in Atomic Absorption Spectrophotometer ICPE-9000. Iron, Na, K and Zn in leaf tissues of pea plants versus increasing phytonematicides concentration, regardless of the growing condition, exhibited significant (P ≤ 0.05) negative or positive quadratic equations. In conclusion, results suggested that the priming-and-drying technology could affect pea plant growth through disproportionate accumulation of nutrient elements in foliar leaf tissues.

The Effect of Exogenous Amylase Supplementation on the Nutritional Value of Pea (Pisum sativum L.) for Broiler Chickens

The present study aimed to investigate whether the exogenous addition of amylase enhances the nutritional value of pea seeds for broiler chickens. In total, 84 1-day-old male broiler chickens (Ross 308) were used for the experimental study. During the first phase of the experiment (1-16 d), all birds in each treatment were fed with a corn-soybean meal reference diet. After this time, the first treatment (control) was still fed the reference diet. In the second and third treatment, 50% of the reference diet was replaced with 50% pea seeds. In addition, the third treatment was supplemented with exogenous amylase. Animal excreta were collected on 21 d and 22 d of the experiment. The birds were sacrificed at the end of the experiment (23 d), and samples of ileum content were collected. The experimental results showed that the exogenous addition of amylase significantly improved (p < 0.05) the apparent ileal digestibility (AID) of the crude protein (CP), starch, and dry matter (DM) of pea. In addition, an improvement in the AID of essential amino acids in pea seeds (except Phe) was observed. The trend in the AMEN values was also noted (p = 0.076). It can be concluded that supplementation with exogenous amylase improves the nutritional value of pea seeds in broiler chicken nutrition.

The effect of reduction of resistant starch content of faba bean and pea by amylase supplementation on performance, nutrient digestibility, and sialic acid excretion of broiler chickens

The aim of the present study is to investigate the impact of the protein source and amylase addition and the interaction between these two on growth performance and nutrient utilization in particular starch digestibility and AMEN value in broiler chickens. A total of 960 one-day-old male broiler chickens (Ross 308) were utilized in this study. The chickens were randomly classified into four experimental groups, with each group having 30 replications, with eight birds per replication. The groups differed from each other with respect to the source of crude protein in diets (pea or faba bean seeds) and the addition of exogenous amylase (not added or added). The chickens had unlimited access to feed and fresh water and were kept under stable environmental conditions. The body weight and feed intake of the chickens were measured on the 14th and 35th days, with the aim of determining growth performance parameters. At the end of the study, the chickens were sacrificed and samples (excreta and digesta) were collected to estimate the nutritional value. The results showed no interaction (P > 0.05) between the protein source and enzyme addition in any of the analyzed growth performance and nutritional value parameters. The addition of amylase resulted in a higher body weight gain (0–35 days) and feed conversion ratio (each period). Moreover, significantly higher (P < 0.05) dry matter, starch, and amino acid digestibility were observed. Amylase addition resulted in lower excretion of total sialic acids (P < 0.05). The faba bean diet improved the growth performance and had greater nutritional value. In conclusion, amylase addition improved the growth performance and starch and amino acid digestibility. Therefore, it seems that the use of exogenous amylase is effective regardless of the source of protein in broiler chicken nutrition.