Indica Seeds Research Articles

Effect of microencapsulated/photoresistant extract of Azadirachta indica A. Juss seeds on Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctudae) and its persistence in semi-field

The development of bioinsecticides as an alternative to synthetic insecticides can benefit the economy of exporting and importing countries, family farming, production of high-quality commodities, food security, quality of life, and the natural environment. This study aimed to evaluate the effect of microencapsulated extracts of Azadirachta indica A. Juss on Spodoptera frugiperda J.E. Smith and their persistence in the plant under semi-field conditions. The fresh seeds of A. indica, collected in Brazil, were separated for the preparation of the microencapsulated dry extract (MDE) according to criteria registered in the patent. Lethal concentrations for S. frugiperda were determined using a dose response curve. The treatments were compared: MDE, control and positive control based on azadirachtin, evaluating mortality and growth inhibition. There was a significant difference in mortality between the treatments tested. The lethal concentrations LC50 and LC95, expressed in azadirachtin for the bioinsecticide, were 194.98 and 630.95 ppm, respectively. The growth inhibition of S. frugiperda was above 98 % in the treatments, except for the control. The MDE presented lethal times LT50 and LT90 lower than the other treatments (lethal time LT50 = 2.79 d; lethal time LT90 = 3.97 d). The MDE of A. indica seeds was effective in controlling S. frugiperda up to the fifth instar and is considered as slightly persistent to persistent depending on environmental conditions and irrigation, based on IOBC/WPRS parameters. The MDE has the potential to be a phytosanitary product approved for use in organic agriculture and with greater effectiveness in controlling S. frugiperda compared to similar pesticides.Abbreviations: BOD, Biochemical Oxygen Demand; CPA, commercial product based on azadirachtin; CLE, concentrated liquid extract; DAS, days after spraying; GI, growth inhibition; LC50, lethal concentration for 50% of the population; LC95, lethal concentration for 95% of the population; LT50, lethal time for 50% of the population; LT90, lethal time for 90% of the population; MDE, microencapsulated dry extract.

Sub-Acute Toxicity Assay of the smoke of Seeds of Azadirachta indica and Peels of Citrus sinensis used as natural insecticides on the Liver, Kidney, Lungs and Heart of Albino Rats

Abstract Natural plant parts such as seeds of Azadirachta indica (neem seeds) and peels of Citrus sinensis (sweet orange) have been used as house-hold remedies against various ailments and bio-pesticides for agricultural purpose due to their repellent, growth regulatory and anti-feedant properties against insects. Despite their beneficial role, these natural insecticides could also have toxic effects on some vital organs of human. Therefore, this study aimed to investigate the sub-acute toxicity of smoke of the Seeds of Azadirachta indica and Peels of Citrus sinensis on the liver, kidney, lungs and heart of swiss albino rats. In this study, three groups of white swiss albino rats were exposed to the smoke of A indica seeds, peels of C sinensis and their combination at different duration of one hour and two hours respectively. The Control group were not exposed to any of the natural insecticides and were fed normal diet and water. After four weeks of the treatment, the animals were all sacrificed and their liver, kidneys, lung and heart were evaluated using standard biochemical and histopathological techniques. The levels of serum biochemical indices for liver (aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin and protein), kidneys (urea, creatinine and electrolytes), and heart (creatine kinase and lactate dehydrogenase) were found to be significantly higher at (p<0.05) for all the groups treated with the two natural insecticides when compared to control group. Histopathological changes including fibrosis, hemorrhages, vascular congestion and necrosis were observed in the liver, kidneys and lungs tissues of the rats exposed to the smoke of the seeds of A indica. However, no histopathological changes were observed in all organs of rats exposed to the smoke of the peels of C sinensis compared to control group. Moreover, the severity of the effects of smoke of these natural insecticides on both biochemical and histopathological indices increases with increase in duration of exposure. The results of this study revealed that seeds of A indica have significant toxic effects on both the tissue and biochemical markers of all the major organs of the Swiss albino rats, while peels of C sinensis shows effect on only the biochemical indices at the specific duration of time studied.

Role of Hydroalcoholic Extracts of Aegle marmelos (AM) Leaves and Tamarindus indica (TI) Seeds on High Fat High Sugar Diet Induced Hyperlipidemia in Rodent Models

Hyperlipidemia is an increase in plasma levels of lipids (cholesterol, triglycerides) and various lipoproteins. Though many drugs have been used for controlling hyperlipidemia, yet most of them have unpleasant side effects, which has stimulated the search for natural remedies. Hypolipidemic activity of hydroalcoholic extracts of Aegle marmelos (AM) leaves and Tamarindus indica (TI) seed alone and in combination on High Fat High Sugar (Fructose) Diet (HFHSD) induced hyperlipidemia in male rats was evaluated in this study. Out of 54 male Sprague Dawley rats, six received standard diet (Group I, normal control) throughout the study. The remaining 48 rats were fed orally with HFHSD for 30 days to induce hyperlipidemia (plasma cholesterol level >200 mg/dL). For the next thirty days, rats which had received HFHSD were divided into 8 groups with six animals in each. Group II- HFHSD control received standard diet, Group III- positive control (Niacin, 100 mg/kg/day), Group IV - AM25 (25 mg/kg/day), Group V - AM50 (50 mg/kg/day), Group VI - TI25 (25 mg/kg/day), Group VII - TI50 (50mg/kg/day), Groups VIII - AM25+TI25 (25 + 25 mg/kg/day) and Group IX - AM50+TI50 (50 + 50 mg/kg/day). Treatment of HFHSD fed rats with each extract alone and in combination resulted in a significant decrease in plasma cholesterol, triglycerides, LDL and VLDL and increase in HDL levels. Treatment with AM50+TI50 significantly lowered plasma cholesterol (p<0.001), triglycerides (p<0.001) and increased HDL cholesterol levels (p<0.05), in comparison to positive control. Both the extracts alone and in combination exerted hypolipidemic effect in rats.

Behavioural assay of a newly identified lepidopteran, Pexicopia tungabhadrai (Lepidoptera: Gelechiidae) along with a first report of larval diapause

Abstract This study examined the ovipositional and olfactory preferences of gravid pod moths in both choice and non-choice settings. In terms of olfactory preferences, pod moth neonates strongly preferred Abutilon indicum under choice conditions, showing nil interest in other hosts. Under non-choice conditions, both neonates and final-instar larvae thrived on A. indicum but refrained from feeding on other hosts or diets, leading to neonate mortality. Remarkably, neonates exhibited an absolute preference for A. indicum seeds, being unable to feed on Abutilon seed diets or green cotton bolls, resulting in starvation-induced mortality. A majority of the final-instar larvae successfully fed, entered pupation and completed their life cycle on A. indicum seeds but the majority entered diapause when exposed to other hosts or diets as compared to A. indicum at a temperature of 21°C, a relative humidity of 60% and a photoperiod of 14:10 hours (light:dark). Cage studies confirmed pod moth’s strong preference for A. indicum, allowing for successful larval feeding and adult emergence. However, pod moth failed to establish on cotton green bolls, underscoring their exclusive reliance on A. indicum as a host. These findings illuminate the intricate host preferences and behaviour of pod moth, underscoring the pivotal role of A. indicum in their life cycle, and is helpful for further research to exploit pod moth as an agent for biological control of the weed A. indicum.

An integrated biorefinery of Madhuca indica for co-production of biodiesel, bio-oil, and biochar: Towards a sustainable circular bioeconomy

The advancement of non-waste biorefinery technology has proved beneficial in effectively using waste biomass resources. The potential of various biorefinery designs to transform biomass into a range of commercially viable products and bioenergies has piqued attention worldwide among the several scenarios proposed to commercialize biofuels. This paper describes the biorefinery of Madhuca indica (M. indica) seeds to generate biochar, bio-oil, and biodiesel. Pyrolization was used on the de-oiled cake to produce charcoal and bio-oil. The M. indica seed's biochar was utilized as a useful supporting particle to immobilize the enzyme. Through transesterification, the immobilized enzyme transformed the oil extracted from the seed into biodiesel. Using an optimum methanol-to-oil ratio of 7.5:1, 3 % (w/w) of catalyst, and 16 % (v/v) of water content, the method yielded a maximum fatty acid methyl ester (FAME) conversion of 93.4 %. Diesel and 20 % biodiesel have been assessed in terms of performance and emissions analysis. Comparing a 20 % blend of biodiesel to commercial diesel at full load, the emissions of carbon monoxide (CO) were reduced by 21.2 %, hydrocarbon (HC) emissions by 18.02 %, and nitric oxide (NO) emissions by 4.33 %. In addition, the capital flow of the suggested biorefinery was assessed using a techno-economic analysis. The biorefinery process payback period for the biodiesel, bio-oil, and charcoal made from M. indica was determined to be 3.1358 years using the Aspen Plus Economic Analyzer. Techno-economic studies reveal that the biorefinery process is highly lucrative and practical. The current study therefore shows methods to use the Madhuca indica seed biorefinery to produce sustainable energy and byproducts with no net emissions of carbon.

Toxicological profiling of methanolic seed extract of Abutilon indicum (L.) Sweet: in-vitro and in-vivo analysis

Ethnopharmacological relevanceAbutilon indicum, a shrub of the Malvaceae family, is found abundantly in tropical countries like India. A. indicum is widely used for its high medicinal properties. Traditionally, A. indicum seed powder is consumed to treat piles, constipation, chronic cystitis, gonorrhea, gleet, and pregnancy-related problems. Despite having numerous medicinal properties and widespread traditional use of A. indicum seeds, scientific validation, and toxicity studies have yet to be documented. Aims of the studyThe primary objective of this study is to conduct a comprehensive study on phytochemical profiling, in-vitro cytotoxicity, mutagenicity, and in-vivo acute and sub-acute toxicity, and genotoxicity on animal models of methanolic extract of A. indicum seed (MAS). Materials and methodsThe qualitative analysis of MAS was explored through FTIR and HR LC-MS. For in-vitro cytotoxicity, the HEK-293 cell line was used, and the TA100 (Staphylococcus typhimurium) bacterial strain was used for the Ames mutagenicity test. A single oral dose of 250, 500, 1000, or 2000 mg/kg body weight of MAS was given to each male and female rat for acute toxicity study and observed for 14 days for any toxicity signs. In the sub-acute toxicity study, 250, 500, or 1000 mg/kg body weight of MAS was administered orally to each rat for 28 days. The experimental animals were weighed weekly, and general behavior was monitored regularly. After 28 days of the experiment, the rats were sacrificed, and different serum biochemical, hematological, and histological analyses were performed. The blood samples of different doses of MAS were used for genotoxicity study through comet assay. ResultsFTIR analysis found different functional groups, which indicated the presence of phenolics, flavonoids, and alkaloids. HR LC-MS analysis depicts several components with different biological functions. The cell cytotoxicity and Ames mutagenicity results showed minimal toxicity and mutagenicity up to a certain dose. The acute toxicity study conducted in Wistar albino rats demonstrated zero mortality among the animals, and the LD50 value for seed extract was determined to be 2000 mg/kg body weight. Sub-acute toxicity assessments indicated that the administration of seed extract resulted in no adverse effects at dosages of 250 and 500 mg/kg body weight. However, at higher doses, specifically 1000 mg/kg body weight, the liver of the experimental rats exhibited some toxic effects. In the genotoxicity study, minimal DNA damage was found in 250 and 500 mg/kg doses, respectively, but slightly greater DNA damage was found in 1000 mg/kg doses in both male and female rats. ConclusionsThe consumption of A. indicum seed powder is deemed safe; however, doses exceeding 500 mg/kg body weight may raise concerns regarding use. These findings pave the path for the creation of innovative medicines with improved efficacy and safety profiles.

Investigating the effect of solvent on anti-antioxidant properties of Sesamum indicum seeds

Dietary phytochemicals are important bioactive compounds that can scavenge reactive oxygen species. These essential compounds may have antioxidant properties which are known to play a significant role in the treatment and prevention of many chronic diseases. Sesame, an oil-bearing seed, is a well-known promising source of food with both nutritional and therapeutic benefits. As a result, the study aimed to evaluate the antioxidant properties of different solvent extracts of Sesame seeds and to analyse the bioactive compounds present. The seeds were obtained from the local farmers and prepared for analysis. The bioactive compounds present in the seeds were extracted using hexane, ethyl acetate, ethanol, and water. The total phenolic content (TPC), the condensed tannin content (CTC), the total antioxidant capacity (TAC), and the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay were also determined using standard methods. Two chemometric methods, hierarchical cluster analysis (HCA) and Pearson correlation, were employed to evaluate the interdependence of the various parameters and the antioxidant activity. Anti-nutrients such as saponins, alkaloids, phytates, and oxalates were also analysed from the powdered seeds. The study results revealed the presence of anti-nutrients such as phytate (7.691 ± 0.8576 mg/g), oxalate (1.501 ± 0.1375 mg/g), saponins (21.33 ± 4.619 mg/g) and alkaloids (317.33 ± 30.29 mg/g). The study also revealed that the aqueous extract exhibited the highest TPC (17.12 ± 0.041 mg GAE/g of dried extract, p < 0.05) and CTC (64.27 ± 4.711 mg CE/g of dried extract, p < 0.05). Ethanol and hexane had a similar total phenolic content (14.83 ± 0.123 and 14.66 ± 1.474 mg GAE/g of dried extract, respectively, p < 0.05Ethyl acetate had the lowest TPC content. Ethanol extracts had the highest antioxidant activity with a TAC value of 232.6 ± 6.267 mg/g AAE and a DPPH scavenging activity of IC50 of 52.81 ± 2.30 μg/mL. A good correlation (p < 0.05) was established between the extracts' TPC, CTC, TAC, and DPPH radical scavenging activity. Chemometric analysis from the study showed no significant connection between the radical scavenging activity of TPC and DPPH. From the results obtained, it can be concluded that the bioactive compounds present in the sesame seed and their subsequent antioxidant properties are dependent on the nature of the solvent used for extraction.

Evaluation of Repellent Activity of Oils Obtained from the Seeds of Azadirachta indica and Jatropha curcas on the Insect Pest- Aphis craccivora Found in Cowpea

Evaluation of Repellent Activity of Oils Obtained from the Seeds of Azadirachta indica and Jatropha curcas on the Insect Pest- Aphis craccivora Found in Cowpea

Kinetic evaluation of Azadirichtin and related phytoconstituents extraction from Azadirachtaindica (neem) and Melia azedarach (chinaberry) seeds using ultrasound assisted extraction and maceration

The comparative extraction of total phenolic compounds from A. indica and M. azedarach seed powder using two different methodologies is evaluated. The solvent screening was done using soxhlet extraction for six different solvents and methanol had the maximum extraction capacity for both seed powder. Extraction of total phenolic compounds from A. indica and M. azedarach seed powder was done using maceration and ultrasound. The kinetics and extraction mechanism was evaluated using popular three empirical and five semi-empirical models along with scanning electron micrograph analysis. The rate constants showed an enhancement in extraction kinetics of about 4.5 fold for M. azedarach and 7.8 fold for A. indica seeds using ultrasound. Based on model evaluation, extraction using maceration for both seeds follows inter-particle diffusion as the rate-limiting step with non-swelling behaviour. Whereas, in the case of ultrasound, the dependence of rate is on the diffusion of solute within the solid and the solid surface to the solvent. In addition, HPLC and GC-MS analysis suggested differences in the composition of extracts and some important phytochemicals were extracted in larger quantities using ultrasound as compared to maceration.

Extraction of Oil from Azadirachta indica and Moringa stenopetala Seeds and Evaluation of its Physicochemical Properties

Azadirachta indica and Moringa stenopetala trees have been regarded as underutilized, tropical plants, fast-growing, drought-tolerant, robust, oleaginous, and evergreen perennial trees growing widely in various regions of Ethiopia. Almost every part of these plants (i.e., roots, stems, foliage, seeds, and barks) can be used as food additives and as raw materials for pharmaceuticals, cosmetics, soap, and biofuel processing industries. This study aimed at the extraction and characterization of oil from A. indica and M. stenopetala seeds using the solvent method. The Box-Behnken Design was employed in the experimental design and result analysis. The particle size (0.2, 0.5, 0.8 mm), solvent-to-solute ratio (3:1, 6:1, 9:1), and extraction time (2, 5, 8 hrs) were experimental variables with three levels of low, medium, and high, whereas, the extraction temperature was kept uniform. Seventeen experiments were conducted for each species thereby developing the quadratic models with a P-value &lt; 0.0001(significant). The quality and adequacy of the models were evaluated by analysis of variance (ANOVA) at 5% least significant difference. Results of the physicochemical determination of oils were triplicated and obtained as mean ± standard deviation. The determined physicochemical properties of A. indica and M. stenopetala seed oils were kinematic viscosity, specific gravity, pH value, refractive index, acid value, free fatty acid, saponification value, iodine value, and peroxide value. The obtained experimental results showed that the extracted oils from A. indica and M. stenopetala seeds exhibit good oil quality, and hence, they can be employed for commercial and industrial purposes, and the generation of renewable energy (biofuel).

TREATMENT OF INDUSTRIAL WASTEWATER BY USING ECO-FRIENDLY TECHNIQUE

The textile industry is one of the major industries and work as the backbone of economy of Pakistan. It employs thousands of people without any education. It plays important role in the economy of every country around the world. The textile industry has high importance when it comes to the environment because it consumes many environmental resources such as water. Also, it produces a large amount of wastewater which is then discharged into the environment and causes environmental pollution. In this study, Ocimum basilicum L. (sweet basil seeds) and Tamarindus indica seeds have been evaluated as an active natural coagulant for the removal of pollutants from textile wastewater. The change in pH, Dissolved Oxygen, Electrical conductivity, TDS, COD, and heavy metals/pollutants were analyzed. Also, the dye concentration was found to be the most important parameter affecting color removal. Pollutant removal efficiency was obtained by using a low number of coagulants. The mucilage of O. basilicum and the powder of T. indica seeds were found to be highly effective in treating textile wastewater as a sole coagulant. The results show that these coagulants help to reduce heavy metals from 0.16 ppm to 0.02 ppm. Utilization of these natural coagulants was found to be suitable, easier, cost-effective, and environment friendly for textile waste water treatment.

Efficacy of Botanicals against Rice Weevil &lt;i&gt;Sitophilus oryzae&lt;/i&gt; (L) on Stored Sorghum

A laboratory experiment was carried out to evaluate botanicals and locally available natural products against rice weevil Sitophilus oryzae (L). Phytolacca dodecandra (endod) seed, Azadirachta indica (neem) seed, and Allium sativum (garlic) led to more adult mortality as well as had a comparable effect to malathion dust (5%), which completely protected the grains against F1 progeny, while Croton macrostachyus leaf and seed powder caused the lowest adult mortality. However, P. dodecandra leaf and A. indica seeds led to the least amount of seed damage. The germination tests showed that none of the botanicals had an adverse effect.

Mango (Mangifera indica) seeds and peel-derived hydrocolloids: Gelling ability and emulsion stabilization

Mango is a tropical fruit that is consumed worldwide and is distinguished by its sweet and juicy flesh. Unfortunately, the peels and seeds of mango fruit are often discarded despite the fact that they contain compounds that can be valorized for certain applications. In this study, a physicochemical and hydrodynamic characterization of hydrocolloids extracted from the peel and seeds of mango fruit was performed and the impact on the microstructure and rheological behavior of derived gels, as well as the ability to form emulsions, were analyzed. The physicochemical parameters and proximate compositions of the different samples showed that the hydrocolloids extracted from the seeds are rich in proteins whereas those from the peel are rich in carbohydrates. The molecular weights calculated from the hydrodynamic characterization (Mv) are 101515 g/mol and 102,580 g/mol for hydrocolloids extracted from the peel and seeds, respectively. Dispersions of these hydrocolloids in water produced hydrogels upon heating, which exhibited a shear thinning response that can be described by the Williamson model. The ability of these hydrocolloids to form thermally-induced strong gels was monitored and analyzed by means of curing tests. Throughout thermal treatments, the storage modulus showed a significant increase, especially at concentrations greater than 2.5 wt%. Mango seeds and peel-derived hydrocolloids are also able to stabilize o/w emulsions. Derived emulsions showed D50 mean diameters and zeta potential values (ζ) in the ranges from 10.04 to 82.52 μm and from -17.63 to -11.12 mV, respectively. On the basis of the observed rheological behavior of hydrogels and emulsion-forming ability, the hydrocolloids derived from mango seeds and peels possess suitable characteristics to be potentially implemented in diverse food matrices, offering functional and nutritional benefits to final consumer products.

Tamarindus indica seed polysaccharide-copper nanocomposite: An innovative solution for green environment and antimicrobial studies

The purpose of this study was to synthesize ecofriendly nano-composite in which agricultural waste (seeds of Tamarindus indica) was used to synthesize tamarind seed polysaccharides (TSP) and its composite with copper nanoparticles (Cu-NPs) for the purpose of green and clean environment as well as reduction of green-house gases. Confirmation of extracted TSP, synthesized nanocomposite was carried out using FTIR, SEM, PXRD and EDX techniques. In FTIR analysis TSP gives a strong broad peak at 3331 cm−1 due to –OH group and in case of composite its intensity is reduced which might be due to the interactions between –OH and Cu+2 ions. SEM analysis gives that TSP have irregular and rough surface while Cu-NPs exhibited spherical morphology and composite showed clustering of spherical shape to rough surface. EDX analysis quantitatively represented copper having atomic ratio 0.57 % which confirms the synthesis of composite. Furthermore, synthesized composite demonstrated excellent antibacterial activity against gram-positive (S.aureus) and gram-negative bacteria (E.coli) even greater than standard medicine (ciprofloxacin). From this study it was revealed that agriculture waste can be utilized to make environment green as well as synthesized composite from agricultural waste seed also displayed excellent antimicrobial activities which directs that they can be utilized in medical field. This study aims to assess the antimicrobial properties of the nanocomposite, aiming to contribute to the development of effective antimicrobial agents. Through these objectives, the research seeks to bridge the gap between green technology and antimicrobial efficacy, offering a promising avenue for both environmental conservation and healthcare advancements.

Biomass valorization of Mangifera Indica into hierarchical carbon materials with self-doping oxygen assisted carbonization for supercapacitor applications

ABSTRACT The exploration of biocompatible energy storage media for future generations has been continuously carried out. This includes the investigations of electrode materials that are renewable, environmentally friendly, cost-effective, and rich in heteroatoms. The electrode materials undergo carbonization as a promising thermochemical process to create an active material with a porous structure of well-defined and controlled geometry. The main objective of this study is to thoroughly examine the conversion process of electrode materials derived from Mangifera Indica Seed Shells (MIS) using carbonization and activation methods for supercapacitor applications. Pyrolysis at various temperatures was carried out to determine the definitive influence of carbonization temperature on the capacitive performance of MIS active materials. The optimum carbonization temperature of 600°C significantly enhances mesoporosity, which is positively correlated to the specific surface area, hierarchical structure, and the presence of self-doping oxygen, leading to a substantial increase in capacitive behavior. In the experiment, the MIS samples were used as electrodes for supercapacitor cells, and they were found to exhibit a specific capacitance of 255 Fg−1. The goal of this study is to offer a sustainable solution by using biomass as an active material for high-performance supercapacitor cells that can be utilized in energy storage applications.

Facile cost-effective green synthesis of carbon dots: selective detection of biologically relevant metal ions and synergetic efficiency for treatment of cancer

A facile cost-effective green synthesis approach has been used to synthesize carbon-dot (CDs) from the Kernel part of the Azadirachta Indica seeds and investigated their fluorescent and metal ions sensing capability and also used for the delivery of drugs. Metallic ions such as Ca2+, K+, Na+, Fe3+, and Zn2+ which are biologically important for many reactions and are selectively detected through the novel CDs. The resultant dot size of CDs (∼4 nm) is useful to eliminate the ‘Achilles heel’ problems, which is associated with the Zn2+ in the body and its detection is a very challenging task. It is found that the sensitivity of CDs for the detection of Zn2+ can be regulated by using different solvents. These CDs can also be used as a sensing probe for the selective detection of Fe3+ at a very low concentration of solution (∼5 μM). The synthesis method of CDs reported here is cost-effective, very fast and it is highly selective towards Fe3+ and Zn2+. Due to the fast response capability of these CDs, logic gate operation is achieved and it provides a new understanding to construct potential next-generation molecular devices for the detection of different biomolecules with high selectivity. Additionally, these CDs are biocompatible against normal healthy cells, capable of loading small biomolecules and drugs due to their porous nature, and exhibited potential impact for breast cancer therapy. It is observed that a significant synergic therapeutic effect of CDs loaded with doxorubicin against breast cancer cells is very promising. Thus, the CDs reported herein in this work have been synthesized through a green synthesis approach and can be used as a molecular probe for the detection of metal ions as well as for drug delivery applications.

Analysis of genes specific to the early maturation stage of Sesamum indicum seeds by subtraction method*,*.

The mechanisms regulating the content ratio of unsaturated fatty acid in sesame oil need to be clarified in order to breed novel varieties with high contents of unsaturated fatty acids. Full-length cDNA libraries prepared from sesame seeds 1 to 3 weeks after flowering were subtracted with cDNAs from plantlets of 4 weeks after germination. A total of 1545 cDNA clones was sequenced. The functions of novel genes expressed specifically during the early maturation of sesame seeds were investigated by the transformation of Arabidopsis thaliana. Thirteen genes for a transcription factor were identified, four of which were involved in ethylene signaling. Fifty-nine genes, including those for the aquaporin-like protein and ethylene response factor, were analyzed by overexpression in A. thaliana. The overexpression of novel genes and the aquaporin-like protein gene in A. thaliana increased the content of unsaturated fatty acids. The localization of these products was investigated by the induction of the expression vectors for the GFP fusion protein into onion epidermal cells and sesame root cells with a particle gun. As a result, two cDNA clones were identified as good candidate genes to clarify the regulation in the yield and the ratio of unsaturated fatty acids in sesame seeds. Sein60414 (Accession No. LC603128), an intrinsic membrane protein, may be involved in the increase of unsaturated fatty acids, and Sein61074 (Accession No. LC709278) MAP3K δ-1 protein kinase in the regulation of the total ratio of unsaturated fatty acids in sesame seeds.

Ethanolic Extract of Mangifera indica Protects against CCl4-Induced Hepatotoxicity via Antioxidant Capabilities in Albino Rats.

To investigate the antioxidant and hepatoprotective effects of ethanolic Mangifera indica (M. indica) seed extract on carbon tetrachloride (CCl4)-induced hepatotoxicity in albino rats. Forty-eight albino rats weighing (100-150 g) were used for hepatoprotective and toxicity experiments. Antioxidant activity was determined using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The toxicity of M. indica seeds on the liver was evaluated by examining wellness parameters, body weight, and liver histological sections. The protective effects of 50 mg/kg and 100 mg/kg of seed extract on CCl4-induced hepatotoxicity were investigated by evaluating hematological, renal, and liver function parameters, body weight, and liver histological sections. The antioxidant activity of the M. indica ethanolic extract was (92 ± 0.03 RSA %) compared with (91 ± 0.01 RSA %) of propyl gallate, and the IC50 was (8.3 ± 0.01 µg/ml) and (14.1 ± 0.01 µg/ml). No changes were observed in the health indicators, body weights, and liver histological sections following oral administration of 50 mg/kg and 100 mg/kg of M. indica seed extracts. Treatment with M. indica seed extract significantly reduced alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), blood sugar, and urea levels compared with those in the CCl4-treated group. The IC50 of the M. indica ethanolic extract was 8.3 µg/ml, and the M. indica extract is a potential source of natural antioxidants that protect against CCl4-induced hepatotoxicity.

Therapeutic Intervention of Opuntia Ficus Indica (L.) Fruit and Seed Powder against CCl4-Induced Acute Liver Injury in Wistar Rats

Therapeutic Intervention of Opuntia Ficus Indica (L.) Fruit and Seed Powder against CCl4-Induced Acute Liver Injury in Wistar Rats

Effect of Coadministration of Hydroalcoholic Extracts of Aegle marmelos Leaves and Tamarindus indica Seeds on Streptozocin-Nicotinamide Induced Type- 2 Diabetic Rat Model

It is difficult for doctors to control hyperglycemia in type 2 diabetes patients without having an effect that is too detrimental to the patient, despite the advancements that have been made in diabetes medications. Because of their adaptability and capacity to lower blood sugar with very few unintended side effects, herbal preparations are quickly gaining in popularity worldwide. In this study, hydroalcoholic extracts of Tamarindus indica seeds and Aegle marmelos leaves were tested to determine what kind of effect they had on a model of diabetes carried out on rats. For the purpose of this investigation, 54 male Sprague Dawley (SD) rats were used as participants. Group I comprised of six people who did not have diabetes and were considered to be in good health. Intraperitoneal injections of streptozocin (55 mg/kg) and nicotinamide (NAD) (120 mg/kg) were used to induce diabetes in the remaining animals (n = 48). Following that, we divided them up into a total of eight distinct categories. Animals in groups IV and V were given AM at doses of 25 and 50 mg/kg/day, animals in groups VI and VII were given TI at doses of 25 and 50 mg/kg/day, animals in groups VIII and IX were given a combination of the two extracts (AM25+TI25 &amp; AM50+TI50), while diabetic animals were used as controls in group II. A positive control consisting of metformin given at a dose of 200 mg/kg per day was utilized in group III. When compared to the diabetic control group, the fasting blood glucose levels and the area under the curve (AUC) of the oral glucose tolerance test were lower in all treatment groups. When compared to the metformin groups, the performance of the extract combination groups was considerably superior (p 0.01). Additionally, as compared to the diabetic control group, all of the treatment groups had significantly reduced levels of MDA and hepatic G-6-Pase activity (p 0.001). In terms of effectiveness, the combination group performed much better than the metformin group despite the fact that all pharmacological groups showed significant increases in body weight, relative liver weight, HDL level, and liver glycogen content in comparison to the diabetic controls. (p &lt;05). When compared to the diabetic control group, all therapy groups showed an increase in G-6PD activity (p 0.001), catalase levels (p 0.001), and glutathione (GSH) levels (p 0.05). In STZ-induced type 2 diabetic rats, a combination of extracts (AM50+T150) demonstrated the best improvement after 30 days of therapy when compared to metformin and hydroalcoholic extracts (AM and TI) alone. This was the case even when both treatments were administered separately. There was no difference in the circumstances no matter which extracts were utilized.