Effect of Coadministration of Hydroalcoholic Extracts of Aegle marmelos Leaves and Tamarindus indica Seeds on Streptozocin-Nicotinamide Induced Type- 2 Diabetic Rat Model

Abstract

It is difficult for doctors to control hyperglycemia in type 2 diabetes patients without having an effect that is too detrimental to the patient, despite the advancements that have been made in diabetes medications. Because of their adaptability and capacity to lower blood sugar with very few unintended side effects, herbal preparations are quickly gaining in popularity worldwide. In this study, hydroalcoholic extracts of Tamarindus indica seeds and Aegle marmelos leaves were tested to determine what kind of effect they had on a model of diabetes carried out on rats. For the purpose of this investigation, 54 male Sprague Dawley (SD) rats were used as participants. Group I comprised of six people who did not have diabetes and were considered to be in good health. Intraperitoneal injections of streptozocin (55 mg/kg) and nicotinamide (NAD) (120 mg/kg) were used to induce diabetes in the remaining animals (n = 48). Following that, we divided them up into a total of eight distinct categories. Animals in groups IV and V were given AM at doses of 25 and 50 mg/kg/day, animals in groups VI and VII were given TI at doses of 25 and 50 mg/kg/day, animals in groups VIII and IX were given a combination of the two extracts (AM25+TI25 & AM50+TI50), while diabetic animals were used as controls in group II. A positive control consisting of metformin given at a dose of 200 mg/kg per day was utilized in group III. When compared to the diabetic control group, the fasting blood glucose levels and the area under the curve (AUC) of the oral glucose tolerance test were lower in all treatment groups. When compared to the metformin groups, the performance of the extract combination groups was considerably superior (p 0.01). Additionally, as compared to the diabetic control group, all of the treatment groups had significantly reduced levels of MDA and hepatic G-6-Pase activity (p 0.001). In terms of effectiveness, the combination group performed much better than the metformin group despite the fact that all pharmacological groups showed significant increases in body weight, relative liver weight, HDL level, and liver glycogen content in comparison to the diabetic controls. (p <05). When compared to the diabetic control group, all therapy groups showed an increase in G-6PD activity (p 0.001), catalase levels (p 0.001), and glutathione (GSH) levels (p 0.05). In STZ-induced type 2 diabetic rats, a combination of extracts (AM50+T150) demonstrated the best improvement after 30 days of therapy when compared to metformin and hydroalcoholic extracts (AM and TI) alone. This was the case even when both treatments were administered separately. There was no difference in the circumstances no matter which extracts were utilized.