Seedling Establishment Research Articles

Seed priming with potassium nitrate alleviates the high temperature stress by modulating growth and antioxidant potential in carrot seeds and seedlings

Early season carrot (Daucus carota) production is being practiced in Punjab, Pakistan to meet the market demand but high temperature hampers the seed germination and seedling establishment which cause marked yield reduction. Seed priming with potassium nitrate breaks the seed dormancy and improves the seed germination and seedling growth potential but effects vary among the species and ecological conditions. The mechanism of KNO3 priming in high temperature stress tolerance is poorly understood yet. Thus, present study aimed to evaluate high temperature stress tolerance potential of carrot seeds primed with potassium nitrate and impacts on growth, physiological, and antioxidant defense systems. Carrot seeds of a local cultivar (T-29) were primed with various concentration of KNO3 (T0: unprimed (negative control), T1: hydroprimed (positive control), T2: 50 mM, T3:100mM, T4: 150 mM, T5: 200 mM, T6: 250 mM and T7: 300 mM) for 12 h each in darkness at 20 ± 2℃. Seed priming with 50 mM of KNO3 significantly enhanced the seed germination (36%), seedling growth (28%) with maximum seedling vigor (55%) and also exhibited 16.75% more carrot root biomass under high temperature stress as compared to respective control. Moreover, enzymatic activities including peroxidase, catalase, superoxidase dismutase, total phenolic contents, total antioxidants contents and physiological responses of plants were also improved in response to seed priming under high temperature stress. By increasing the level of KNO3, seed germination, growth and root biomass were reduced. These findings suggest that seed priming with 50 mM of KNO3 can be an effective strategy to improve germination, growth and yield of carrot cultivar (T-29) under high temperature stress in early cropping. This study also proposes that KNO3 may induces the stress memory by heritable modulations in chromosomal structure and methylation and acetylation of histones that may upregulate the hormonal and antioxidant activities to enhance the stress tolerance in plants.

Impact of salvage logging on short-term natural regeneration in montane forests of the Alps after large windthrow events

Wind disturbances are one of the main drivers of forest dynamics in Europe, shaping forest stands and modifying the ecosystem services provisioning. Salvage logging is often most common strategy adopted after a high-severity disturbance in managed stands. Understanding natural regeneration dynamics including their interaction with the logging operations, is crucial to understand how forests will be changing under a climate with increasing variability and to design adequate adaptive post-disturbance management strategies. In this study, we focused on 148 stands damaged by storm Vaia (2018). The aim was to analyze natural regeneration dynamics under different logging systems and to investigate influences of site characteristics and disturbance legacies on sapling growth and seedling emergence. The sampling protocol consisted of one transect per stand, perpendicular to one of the intact forest edges, and with a length of 80 m. Along the transect, we collected soil cover, natural seedling and sapling stem density, and deadwood quantity in four sample plots of 3 m radius each at distances of 0, 20, 40, and 80 meters from the edge (592 plots in total). Regeneration species composition was mainly driven by previous stand composition, with some exceptions depending on seed dispersal strategy. Distance from the edge significantly influenced seedlings and saplings occurrence in large gaps and affected the browsing damage percentage, together with deadwood presence. According to GLM’s models, distance from the edge, elevation, and logging methods influenced seedling establishment. At the same time, species characteristics, edge structure, deadwood and logging damages significantly influenced pre-storm seedlings and saplings presence and health. In conclusion site factors, disturbance legacies, and logging strategies are key points to consider in post-disturbance management for a fast forest recovery.

Hot and fast: seed ecology for restoration relevant species in the Argyle region of the east Kimberley, Australia

This study investigates the germination requirements of 12 plant species native to the Argyle region of the east Kimberley, a biodiverse monsoonal tropical region characterized by high temperatures, high evaporation, and episodic seasonal rainfall. The research involved quality assessment of mature seeds, followed by dormancy alleviation and laboratory‐based germination to determine the responses of seeds to a range of temperatures (5–40°C) in terms of germination speed (T10), mean germination time, and maximum germination proportion. Data were then modeled to calculate the optimal temperature to support germination for each species. The results showed that germination rapidly commences in response to a wide range of temperatures typical of the wet season (November–February) in the east Kimberley, though germination for most species was still high (>50%) after exposure to temperatures as low as 15°C. Mean optimal temperature for germination across all species was 25.8 ± 1.5°C, with minimal variation between most species, the exception being Dodonaea physocarpa, which preferred cooler temperatures (Topt = 14.0°C). The speed of germination was also rapid (T10 = 1–3 days) across all species at the optimal germination temperature. The findings suggest that temperature is not a limiting factor for germination in this region and that the onset and intensity of the wet season are the most significant factors determining successful germination, emergence, and seedling establishment. The study underscores the importance of species‐specific understanding of environmental temperatures required for seed germination in seed‐based restoration efforts and informs the planning of direct seeding works, thus enhancing restoration outcomes.

Virtual Reciprocal Garden Assessment of Germination Syndromes for Elymus elymoides ssp. brevifolius and Elymus multisetus

Bottlebrush squirreltail (Elymus elymoides) and big squirreltail (Elymus multisetus) are high-priority species for restoration of millions of hectares of rangeland in the western United States that have been degraded by accelerated wildfire and introduced annual grasses. Previous research has compared potential germination and seedling performance of these species in a common environment and noted significant genetic differentiation in characteristics that are associated with their environments of origin. In this experiment, we used wet-thermal germination models and long-term simulations of seedbed microclimate to conduct a virtual reciprocal-garden analysis of the potential germination response of these species. We confirmed significant species differentiation in germination rate that appears to confer site-specific advantages for initial seedling establishment in their respective habitats of origin. Our results specifically highlight the relative importance of late-fall seeding for the more rapidly germinating E. multisetus in order to avoid early-fall germination and post-germination seedling mortality from freezing conditions in the winter. In contrast, the slower-germinating E. elymoides ssp. brevifolius A is less likely to germinate in the fall and likely avoids inherently harsher winter temperatures in the seedbed. Virtual simulations of this type might lead to identification of complex-trait genetic markers that are associated with intraspecific and interspecific adaptations to specific environments. Identification of these traits could also inform management of plant communities that are under threat from invasive weeds and climate change.

Impact of Foliar Application of Plant Growth Regulators in Soybean [Glycine max (L.) Merill)] on Seed Quality and Seedling Establishment

Impact of Foliar Application of Plant Growth Regulators in Soybean [Glycine max (L.) Merill)] on Seed Quality and Seedling Establishment

Grab to fill the gap: key factors influencing Reynoutria japonica germination and seedling establishment in the secondary distribution range

Reynoutria japonica (Japanese knotweed) is a highly invasive female plant that primarily reproduces through vegetative regeneration during secondary distribution. Despite producing a substantial number of viable hybrid seeds, the occurrence of seedlings and young plants in the wild remains rare; the reasons for this rarity are unclear. Environmental intolerance and chromosomal anomalies during hybridisation can impede seedling development. This study evaluates the combined impact of these factors by examining substrate, temperature, and light effects on germination, as well as assessing seedling genome sizes and viability. Seeds were cultivated in both natural and controlled environments to manage external influences. Flow cytometry was employed to evaluate chromosomal arrangements. The final germination was notably high at 99%, and 68% of seedlings thrived under controlled conditions, emphasising hybrid seedling viability regardless of highly polyploid levels ranging from pentaploid to aneuploid (2C DNA genome size from 5.17 to 11.95 pg). Thus, it is evident that seeds can germinate and produce vital seedlings despite various chromosomal sets. Even fluctuating temperatures and type of substrate do not limit seed germination. However, these results were obtained under laboratory conditions, with seeds and seedlings receiving regular irrigation. A significant seedling mortality rate (99%) was observed in the field experiment, and the final germination was also low there (15%). Observations suggest that water stress might be the cause of this mortality. Further research on water stress is necessary because it could be the primary factor limiting the successful generative spread of the knotweeds.

Effect of drought and pine weevil damage on mechanically protected Norway spruce seedlings

Pine weevils (Hylobius abietis L.) pose a significant threat to conifer seedlings by feeding on the bark, thus damaging or killing seedlings. Historically, insecticides were used to suppress such damage, but were slowly phased out in Sweden due to environmental and health concerns. This study aimed to assess field performance of an alternative protection method: mechanical coating applied to the stem of planted Norway spruce (Picea abies) seedlings. Field trials were conducted on 14 sites in south Sweden, using four different types of mechanical protection (Cambiguard, Conniflex, Ekovax, Hylonox), standard insecticide (Merit Forest), and ambient control. Seven sites were established in the drought year of 2018 and seven more in 2019. This allowed for additional investigation of the effect of drought on seedling establishment and possible interaction with pine weevil damage. Seedlings were surveyed for survival and height after the first, second and fourth growing season. Results show drought as the main source of damage for seedlings planted in 2018, with no significant effect of insecticide or mechanical protection on survival of seedlings. However, mechanical protections performed equally well as insecticide and positively increased survival by 30 %, compared to untreated, four growing seasons after planting for seedlings planted in 2019. Seedling height was not significantly affected by planting year or any of the treatments, suggesting no adverse effects of coating application. However, a synergistic effect between pine weevil damage and drought was observed, where even low levels of pine weevil damage resulted in high mortality for seedlings planted in 2018, compared to those planted in 2019. Additionally, for seedlings planted in 2019, damage to the top of the stem did not result in significant mortality, until high damage levels were reached (40 % and above). The opposite was found for seedlings planted in a 2018 drought year, where both damage to the top and the bottom of the stem followed a linear response. In conclusion, we show that investigated mechanical protection methods can be considered a viable replacement for insecticides, but our results also highlight the importance of considering multiple environmental stressors such as drought and pest damage on seedling establishment.

Seed functional traits as predictors of seedling establishment success in Brazilian tropical forest restoration

AbstractSeveral ecological filters in deforested and degraded areas reduce seedling emergence and establishment and hinder ecological restoration by direct seeding. Understanding whether functional traits are related to a species' capacity to overcome these filters and predict their field performance might improve the success of direct seeding techniques for ecological restoration. We assessed eight seed functional traits of tropical tree species, seeking those that best explained their establishment success in direct seeding restoration projects. We analyzed a dataset from 52 studies that tested direct seeding techniques with tree species in Brazil. From each study, we collected the mean establishment percentage for all tree species. Seed mass, cotyledon function, and germination speed were the only functional traits that significantly affected the species establishment percentage in direct seeding restoration projects. Species with larger seeds, storage cotyledons, and faster germination had higher establishment percentages. Choosing species with these functional traits for seed mixes will provide higher establishment percentages and, consequently, improve restoration success by direct seeding techniques.

Long days induce adaptive secondary dormancy in the seeds of the Mediterranean plant Aethionema arabicum

Secondary dormancy is an adaptive trait that increases reproductive success by aligning seed germination with permissive conditions for seedling establishment. Aethionema arabicum is an annual plant and member of the Brassicaceae that grows in environments characterized by hot and dry summers. Aethionema arabicum seeds may germinate in early spring when seedling establishment is permissible. We demonstrate that long-day light regimes induce secondary dormancy in the seeds of Aethionema arabicum (CYP accession), repressing germination in summer when seedling establishment is riskier. Characterization of mutants screened for defective secondary dormancy demonstrated that RGL2 mediates repression of genes involved in gibberellin (GA) signaling. Exposure to high temperature alleviates secondary dormancy, restoring germination potential. These data are consistent with the hypothesis that long-day-induced secondary dormancy and its alleviation by high temperatures may be part of an adaptive response limiting germination to conditions permissive for seedling establishment in spring and autumn.

Seed priming as a promising technique for sustainable restoration of dryland

The success of plant growth and development in dryland restoration relies on seed germination, seedling development, and establishment. The lack of rainfall and extreme temperature variations in arid regions impede germination and seedling establishment, necessitating new approaches and techniques. The present review summarizes the vital role of seed priming techniques for the sustainable restoration of drylands through their effects on enhancing seed germination, seedling growth and development, plants' nutrient use efficiency, soil fertility, essential enzymes, microbial community, and stress adaptation ability, helping to alleviate the effects of abiotic stresses on plants. The importance of seed priming is discussed in both agricultural and ecological restoration contexts. Priming maximizes crop yields, reduces crop failure, and promotes eco‐friendly farming practices. In ecological restoration, seed priming enhances plant establishment and survival, contributing significantly to the restoration of native vegetation and wildlife habitats. The review categorizes seed priming techniques into six subcategories, highlighting their unique advantages for optimizing restoration efforts. The review highlights the underlying mechanisms of seed priming techniques to improve seed germination and plant development in dryland regions, particularly under salinity and drought constraints. While seed priming has proven effective, it also faces several limitations, such as cost, seed longevity, and environmental concerns. Investigating environmentally friendly priming methods and understanding the prolonged effects of priming on seed viability and the environment is crucial. Further research is needed to find cost‐effective, eco‐friendly seed priming methods, and to explore the genetic and molecular bases of stress tolerance in various plant species across diverse environments.

Natural regeneration of cork oak forests under climate change: a case study in Portugal

The sustainability of forest species is directly related to the success of stand regeneration. Assuring success is particularly critical in stands where perpetuity relies on natural regeneration, as is often the case with cork oak forests. However, 59% of the stand in Portugal have no natural regeneration, and climate change could further worsen the sustainability of the system. The study summarizes the factors that affect the natural regeneration of cork oak (Quercus suber L.) based on current knowledge and presents a case study on a forest in Northeast Portugal, where the natural regeneration of Quercus suber under the effect of climate change have been monitored and analyzed. The present work focuses on the effect of stand density, i.e., tree cover, on the production of acorns, the establishment and survival of seedlings, and the impact of the summer season on seedling mortality. The monitoring was carried out in February, June, September 2022, and January 2023 in two stands with distinct stand canopy cover, when the region was under extreme drought. Data analysis was performed using the analysis of variance for repeated measures and the Mann–Whitney-Wilcoxon test. The study showed that cork oak regeneration is influenced by stand density, which promoted the establishment success and survival of natural regeneration in a period of reduced precipitation, despite possible competition for water resources. The mean number of seedlings differed significantly between the two stands. However, there were no significant differences in the mean number of seedlings throughout the field measurements. Additionally, the percentage of dead seedlings was low even after the summer season (9.5% of the total seedlings) in the denser stand. These results indicate that high canopy cover can have a protective effect for extreme climatic events and should be considered in forestry management to promote regeneration of the cork oak forests.

Beyond fire: Flower production naturally occurs and is also influenced by leaf removal in a Neotropical savanna herb.

Several herbaceous species exhibit mass flowering after fires in Neotropical savannas. However, unequivocal evidence of fire dependency and the consequences for plant reproduction are lacking. In nutrient-poor fire-prone savannas, the damage caused by fire and by other means (e.g., leaf removal, but not necessarily having a negative impact) constrains the maintenance and expansion of plant population by affecting the ability of individuals to recover. Therefore, the compensatory responses of plants to both damages should be convergent in such environments. Using Bulbostylis paradoxa-reported to be fire-dependent to flower-as a model, we investigated the role of fire and leaf removal in anticipating the flowering and reproduction periods, and its possible consequences on seedling establishment. We monitored 70 burned individuals, 70 damaged/clipped, and 35 without damage to estimate time for flowering, seed quality and germination parameters. To expand our sampling coverage, we examined high-resolution images from herbarium collections in the SpeciesLink database. For each herbarium image, we recorded the presence or absence of a fire scar, the month of flowering, and the number of flowering stalks. Bulbostylis paradoxa was fire-stimulated but not dependent on fire to flower, with 65.7% of the individuals flowering in the burned area, 48.6% in the clipped, and 11.4% in the control. This was consistent with the analysis of the herbarium images in which 85.7% of the specimens with flowers had fire scars and 14.3% did not. Burned individuals synchronized flowering and produced more viable seeds. However, the seeds might face a period of unsuitable ecological conditions after early to mid-dry season fires. Flowering of unburned plants was synchronized with the onset of the rainy season. Flexibility in flowering and vegetative reproduction by fragmentation confer to this species, and most likely other plants from the herbaceous layer, the capability of site occupation and population persistence in burned and unburned savanna sites.

Effects of warming on the seedling adaptation and growth of Machilus gamblei in a subtropical forest

Accurate predictions of community stability feedback to climate warming depend on an advanced understanding of responses of the key species. The transition from seed germination to seedling establishment is the most vulnerable stage of plants, which determines the maintenance of plant diversity and community resistance to climate change. To elucidate how the establishment and physiological characteristics of Machilus gamblei seedlings will respond to future global warming, artificial warming experiments (infrared warming, +0.75 ℃, +1.5 ℃, +3 ℃, +4 ℃) were conducted at the Ailao Mountain National Nature Reserve in Yunnan Province, China. The responses of seedling establishment and their physiological adaptation to different warming conditions were studied, and the contents of nutrients, chlorophyll, and nonstructural carbohydrate (NSC) in the leaves were measured. The results showed that: (1) increasing the temperature by 0.75 ℃ did not affect the survival rate or physiological characteristics of the seedlings, but increasing the temperature by 4 ℃ reduced the survival rate of the seedlings; (2) As the temperature increased from 1.5 ℃ to 3 ℃, the chlorophyll production in the seedling leaves improved, thus particularly promoting the accumulation of NSC by increasing the starch content. In summary, our work suggests that the temperature increase in 1.5 ℃ and 3 ℃ might be conducive to the establishment and growth of Machilus gamblei seedlings in the two-year experimental period, but increasing the temperature by 4 ℃ would threaten their survival and development.

Factors limiting the potential range expansion of lodgepole pine in Interior Alaska.

Understanding the factors influencing species range limits is increasingly crucial in anticipating migrations due to human-caused climate change. In the boreal biome, ongoing climate change and the associated increases in the rate, size, and severity of disturbances may alter the distributions of boreal tree species. Notably, Interior Alaska lacks native pine, a biogeographical anomaly that carries implications for ecosystem structure and function. The current range of lodgepole pine (Pinus contorta var. latifolia) in the adjacent Yukon Territory may expand into Interior Alaska, particularly with human assistance. Evaluating the potential for pine expansion in Alaska requires testing constraints on range limits such as dispersal limitations, environmental tolerance limits, and positive or negative biotic interactions. In this study, we used field experiments with pine seeds and transplanted seedlings, complemented by model simulations, to assess the abiotic and biotic factors influencing lodgepole pine seedling establishment and growth after fire in Interior Alaska. We found that pine could successfully recruit, survive, grow, and reproduce across our broadly distributed network of experimental sites. Our results show that both mammalian herbivory and competition from native tree species are unlikely to constrain pine growth and that environmental conditions commonly found in Interior Alaska fall well within the tolerance limits for pine. If dispersal constraints are released, lodgepole pine could have a geographically expansive range in Alaska, and once established, its growth is sufficient to support pine-dominated stands. Given the impacts of lodgepole pine on ecosystem processes such as increases in timber production, carbon sequestration, landscape flammability, and reduced forage quality, natural or human-assisted migration of this species is likely to substantially alter responses of Alaskan forest ecosystems to climate change.

Seed physiological traits and environmental factors influence seedling establishment of vegetable soybean (Glycine max L.).

Edamame (Glycine max (L.) Merr.), a specialty soybean prized for its nutritional value and taste, has witnessed a surge in demand within the U.S. However, subpar seedling stands have hindered its production potential, necessitating increased inputs for farmers. This study aims to uncover potential physiological factors contributing to low seedling emergence in edamame. We conducted comprehensive assessments on thirteen prominent edamame genotypes alongside two food-grade and two grain-type soybean genotypes, focusing on germination and emergence speed in both laboratory and field settings. Additionally, we employed single electrical conductivity tests and identified and quantified seed leachate components to distinguish among soybean types. Furthermore, using a LabField™ simulation table, we examined seed emergence across a wide soil temperature range (5°C to 45°C) for edamame and other soybean types. All seeds were produced under the same environmental conditions, harvested in Fall 2020, and stored under uniform conditions to minimize quality variations. Our findings revealed minimal divergence in emergence percentages among the seventeen genotypes, with over 95% germination and emergence in laboratory conditions and over 70% emergence in the field. Nonetheless, edamame genotypes typically exhibited slower germination speeds and higher leachate exudates containing higher soluble sugars and amino acids. Seed size did not significantly impact total emergence but was negatively correlated with germination and emergence speed, although this effect could be mitigated under complex field conditions. Furthermore, this study proposed differences that distinguish edamame from other soybean types regarding ideal and base temperatures, as well as thermal time. The finds offer valuable insights into edamame establishment, potentially paving the way for supporting local edamame production in the U.S.

Characterization of tadpole shrimp, Triops longicaudatus (Notostraca: Triopsidae), injury to rice and its effect on rice seedling establishment.

Rice seedlings of different stages of development were infested with tadpole shrimp (TPS, Triops longicaudatus [LeConte]), of different sizes in four experiments conducted in a lath house in Colusa, CA, during 2015 and 2016. Tadpole shrimp size was based on carapace length. Rice seedling stages were classified as S0 if no structures were present; S1 if only the coleoptile was present; S2 if the coleoptile and radicle were present; S3-I if the prophyll had emerged; S3-II if the first true leave had emerged but was not unfurled; and V1 if the first true leave was unfurled. Results showed that TPS of all sizes are capable of injuring rice and reducing seedling establishment, with larger TPS causing more seedling establishment reduction than medium or small TPS. The coleoptile, radicle, and roots were the structures most injured by TPS. During the S0 stage, TPS buried seeds, preventing their establishment. Seedling stages S1, S2, and S3-I were found to have the highest risk of injury by TPS, while seedling stage V1 had the lowest. Pearson's correlation between injured seedlings and the normalized number of established seedlings indicates that injury to the coleoptile, root, and radicle combined are the most important in preventing seedling establishment.

Factors associated with seedling establishment on logs of different fungal decay types-A seed-sowing experiment.

Wood decay fungi alter the abiotic and biotic properties of deadwood, which are important as nurse logs for seedling regeneration. However, the relationship between fungal decay type and seedling performance has not been evaluated experimentally. In this study, we examined the germination, growth, and survival of six arbuscular mycorrhizal (AM) and six ectomycorrhizal (ECM) tree species on three substrates (pine logs with brown and white rot and soil) by conducting seed-sowing experiments in a mixed forest dominated by Pinus densiflora and Quercus serrata. Analysis using ribosomal DNA internal transcribed spacer 1 (rDNA ITS1) sequencing revealed that the fungal community was significantly different across three substrates. The richness of operational taxonomic units (OTUs) of AM and ECM fungi was the largest on brown rot logs and soil, respectively. The substrate significantly affected the seedling performance when comparing wood decay types, but these were not consistent across the mycorrhizal status of the seedlings. Nevertheless, seedlings of some AM trees showed better growth and enhanced mycorrhizal colonization on brown rot logs than on white rot logs. The wood decay type influenced fungal communities in the logs and the performance of some seedling species partly by different mycorrhizal colonization rates. However, the effect was seedling species dependent and showed no apparent difference between AM and ECM trees.

Seed traits and burial state affect plant seed secondary dispersal mediated by rodents

Seed dispersal is an important ecological process and has important implications for plant population expansion and regeneration. Seed dispersal not only reduces the probability of death due to seed density but also facilitates seedling establishment. Many studies have focused on the effect of one or two factors on seed dispersal. However, little is known about studies on the effect of multiple factors and their interactions on seed dispersal. Here, we conducted a field experiment to explore how seed size, soil burial, and seed peeling affect the dispersal and hoarding of seeds of Quercus liaotungensis in dispersal animals. We found that large seeds were preferentially selected by animals, and the predation after dispersal, hoarding after dispersal, predation distance after dispersal, and hoarding distance after dispersal of large seeds were significantly greater than small seeds, which is more beneficial to the plant expansion and regeneration. Soil burial increased the time of seed intact in situ, significantly increased predation in situ, and reduced predation after dispersal, predation distance after dispersal, and hoarding distance after dispersal, which is not beneficial to the plant population expansion and regeneration. Seed peeling reduced the time of seed intact in situ, and the predation after dispersal was significantly greater than that of unpeeled seeds, which is not beneficial to the plant population. We did not find the interactions between seed size, soil burial, and seed peeling on dispersal. The effects of a single factor may be more than their interactions between seed size, soil burial and seed peeling on dispersal. These results implied that seed size, soil burial and seed peeling may affect plant population expansion and regeneration by affecting the dispersal and hoarding of animals.

Biochemical and Physical Screening Using Optical Oxygen-Sensing and Multispectral Imaging in Sea Oats Seeds

Physical, physiological, and biochemical traits control critical seed functions such as germination, longevity, persistence, and seedling establishment. These traits are diverse between and among species, and they are also controlled by the environment in which the seed originated. Therefore, screening seed traits and understanding their roles in seed functions is crucial to facilitate the economical use of resources in collecting, sorting, and conserving seed materials of agronomical and ecological importance. We hypothesized the existence of physical and biochemical traits in sea oats seeds that can be used as proxies to predict viability and vigor to develop underpinnings for survival after non-optimal storage conditions. Using multispectral imaging and optical oxygen-sensing analyses, we evaluated the physical and biochemical traits of Uniola paniculata L. (sea oats) seeds collected from the US Atlantic and Gulf coastlines. Our results showed that several traits correlate to aging stress survival in sea oats seeds. These results confirm the potential of using physical and biochemical screening to predict seed quality while offering insights into extended seed longevity periods. Therefore, exploring and analyzing the physical and biochemical properties of seeds could reveal salient markers that contribute to viability and longevity.

Correction to: Allelopathic potentials of surrounding vegetation on seedling establishment of alpine cushion Arenaria polytrichoides

Correction to: Allelopathic potentials of surrounding vegetation on seedling establishment of alpine cushion <i>Arenaria polytrichoides</i>