Vigor Of Seeds Research Articles

The Relationship between Seed Vigor and Germination Performance under Various Chloride Salts in Pea

Background: Salinity is a severe abiotic stress resulting in inhibiting germination, seedling growth of crop plants. Germination performance under salinity depends on seed vigor and genotypic factors. This study aimed to determine if there was a relationship between germination and seedling growth of eight pea cultivars subjected to three chloride salts and the results of seed vigor tests. Methods: Pea cultivars Ambassador, Bolero, Electra, Esprit, Puccet, Rainer, Utrillo and Winner were germinated in the medium with three chloride salts of NaCl, MgCl2 and CaCl2 with the electrical conductivity (EC) of 20 dS m-1. Also germination percentage, mean germination time, controlled deterioration (CD) and electrical conductivity (EC) test were evaluated. Result: The seed vigor of pea cultivars was significantly different and salinity led to a significant decline in parameters of the cultivars. The most hazardous salt was MgCl2, while the least harmful was NaCl. Germination percentage was negatively related with MGT and EC values and EC test was strongly correlated with CD test. Utrillo and Winner were the most sensitive cultivars to MgCl2 and CaCl2. Although germination percentage of Rainer under non-saline was the lowest with 76%, it showed better performance under salinity than Utrillo and Winner. Germination percentage and CD gave a negative significant correlation with NaCl, but MGT and EC associated positively with NaCl. It was concluded that seed vigor should be considered as an indicator for the germination performance of pea seeds under NaCl, whereas genotypic factors play a key factor for tolerance to salinity during germination and early development stages.

Cenários de mudanças climáticas influenciando a germinação e vigor de sementes de Mimosa tenuiflora e Cenostigma pyramidalis

<span class="fontstyle0">Climatic changes occurring on the planet can cause problems in the physiological behavior of plant seeds from the Caatinga biome. The objective of this research was to evaluate a reference scenario to climate changes in the germination and vigor of Mimosa tenuiflora and Cenostigma pyramidalis seeds. For this, different treatments of environmental stress (combinations of temperature, relative humidity, luminous intensities, and water stresses) were tested on seeds of M. tenuiflora and C. pyramidalis. Using Gerbox boxes with vermiculite substrate, the assays were assembled in CRD, and evaluated for variations related to germination and seed and seedlings vigor. The results obtained were obtained by ANOVA and by the Scott-Knott means test. The germination and vigor of M. tenuiflora seeds and seedlings were reduced after a temperature of 30°C. C. pyramidalis seeds germinated only under conditions of alternating temperature, absence of water stress and cannot tolerate temperatures above 30°C. Climatic changes can affect the germination and vigor of the studied species. Research with this theme must be carried out to improve the management of these species in relation to the effects of climate changes.</span> <br /><br />

Piptadenia moniliformis Benth. seeds subjected to accelerated aging

The accelerated aging test is efficient for evaluating the seed vigor of many species, but there are few studies on forest tree species. The aim of this study was to determine the adequate exposure time of Piptadenia moniliformis Benth. seeds to the accelerated aging test in order to evaluate the vigor of seeds classified by color and size. Ten sub-lots were obtained by separating the seeds into two tegument colors (light and dark) and five size classes (11, 12, 13, 14, and 15), using round wire sieves. Before the accelerated aging (AA) test, the seeds were treated with concentrated sulfuric acid for 20 minutes to overcome dormancy. Next, the sub-lot seeds underwent different aging times (0, 24, 48, 72, 96 and 120 hours) at 45 ºC. The AA test, conducted at 45 ºC for 72 hours, is adequate to evaluate the quality of P. moniliformis seeds, in terms of color and size.

Study of the effectiveness of hydrogen peroxide in improving the germination characteristics of maize seed under water stress

"A factorial experiment was carried out in the lab. college of Agricultural Engineering Science, University of Baghdad Al- Jadiriyah during year of 2017 With the aim of improving the germination properties of Maize variety of Noor under water stress. According to the Completely randomize design (CRD) with three replications were used in both and two factors: first: it included water tensile treatments by exposing the seeds to a solution of polyethylene glycol PEG 6000 with three levels (0, -4 ,-8) Bar, while the second factor with three concentrations of the compound peroxide Hydrogen H2O2 with a concentration of 50% (0, 10 , 20 mg L-1). The results showed that water stress had a significant effect on the characteristics of Maize seedlings, as it reduced the level of water stress (-8) bar, the average germination percentage, Average 80.00%, Root Shoot length, 7.900 , 5.22 (cm), respectively, seedling dry weight 269.9 (mg seedlings-1) and Seed vigor index of drought resistance 0.781,while treatment with compound hydrogen peroxide H2O2 contributed to improving the characteristics of Maize seedlings significantly, as the seed soaking treatment at a concentration of 20 (mg L-1) recorded the highest germination rate of 89.44%, the feather length of 5.90 (cm), and the seedling dry weight 353.6 (mg seedlings-1). Germination and vigor index of drought resistant 1.089. We recommend to soak maize seeds with peroxide Hydrogen H2O2 (10 ) mg L-1 for 24 h before planting, especially under water stress."

Controlled deterioration test to evaluate the physiological potential of sweet corn seeds

The evaluation of the physiological potential of sweet corn seeds is essential in a quality control program and the choice of vigor tests using an adequate analysis methodology is essential to obtain reliable results. In this sense, this study aimed to standardize the methodology of the controlled deterioration test through combinations between time and temperature to evaluate the vigor of sweet corn seeds. Six lots of sweet corn seeds were characterized by the degree of moisture and the initial physiological potential through the following tests: germination, first germination count, cold test, electrical conductivity, accelerated aging, length and dry mass of normal seedlings, seedling emergence in sand, and emergence rate index. Subsequently, these lots had their initial water content adjusted to 20% using the wet substrate method. The seeds from the six lots were subjected to the controlled deterioration test under two temperatures for four periods, obtaining a completely randomized design in an 8 × 6 factorial scheme with eight combinations of time/temperature and six lots. Germination and the first germination count were evaluated after the deterioration test. The data were subjected to analysis of variance with a comparison of means by the Tukey test at a 5% significance. The results of the first count and germination tests after deterioration were subjected to a simple linear correlation study together with the initial characterization data. The combinations 8 h – 40 °C and 8 h – 45 °C in the controlled deterioration test were efficient in distinguishing the vigor of seed lots. The shorter exposure period and the first germination count after deterioration are more suitable for evaluating the vigor of sweet corn seeds.

Effects of Maternal Environment on Seed Germination and Seedling Vigor of Petunia × hybrida under Different Abiotic Stresses.

Seed germination and seedling vigor can be affected by environmental cues experienced by the mother plant. However, information about how the maternal environment affects seed quality is scarce in ornamental plants. This study aimed to investigate the effects of two different maternal environments on the seed germination and seedling vigor of Petunia × hybrida under a variety of abiotic stresses. Petunia mother plants were grown in either a greenhouse during the summer months or an indoor controlled-temperature-and-light environment. Collected seeds were subjected to external stressors, including polyethylene glycol (PEG), sodium chloride (NaCl), high temperature, and abscisic acid (ABA), to determine seed germination percentage and seedling vigor. Results indicated that seeds harvested from the mother plants grown in a controlled environment germinated better than seeds harvested from the mother plants grown in the greenhouse when suboptimal germination conditions were applied. Additionally, the seedlings from the controlled maternal environment performed better in both ABA and salinity stress tests than the greenhouse seedlings. Interestingly, the greenhouse seedlings displayed less reactive oxygen species (ROS) damage and lower electrolyte leakage than the controlled environment seedlings under dehydration stress. The difference in germination and seedling vigor of seeds from the two different maternal environments might be due to the epigenetic memory inherited from the mother plants. This study highlighted the strong impact of the maternal environment on seed germination and seedling vigor in Petunia and may assist in high-quality seed production in ornamental plants.

Seed germination and early seedling growth of fenugreek (Trigonella foenum-gracium L.) under Cu, Ni and As stress

Metals and metalloids were known to have a real problem and serious risk to the environment and the ecosystems as well as human beings. In the present experiment, phyototoxicity of essential metals (Cu and Ni) and non-essential metalloids (As) at 10, 50, 100 and 200 mg L−1 were evaluated on seed germination and early seedlings growth of fenugreek. Higher concentrations of Cu had almost no effect on the parameters tested. By contrast, As was found to reduce germination, root and shoot elongation, seeds vigor index and dry biomass within range dose of 50–200 mg L−1. Accordingly, tolerance index showed that fenugreek seeds have zero tolerance at higher concentration of As. The decreasing order of sensibility of fenugreek seeds to tested metals and metalloid followed the order: As > Ni > Cu.

Development of Seed Vigour Testing Method Using Single Count of Radicle Emergence for True Seed of Shallot (Allium ascalonicum B.)

Development of Seed Vigour Testing Method Using Single Count of Radicle Emergence for True Seed of Shallot (Allium ascalonicum B.)

Rhizobacteria improve the germination and modify the phenolic compound profile of pepper (Capsicum annum L.)

The percentage of germination and the vigor of seeds are major determinants for an efficient seedling production to successfully establishment a crop. Some Capsicum annuum germinate within a few days, but others take until 21 days. Pepper seeds have significant amounts of free and bound polyphenols that are modified during the germination process. Phenolic compounds are used by seed to scavenge free radicals, they can also work as signal compounds or they can be related to plant's defense participating in the induced systemic resistance (ISR). However, the effect of plant growth-promoting rhizobacteria (PGPR) on pepper seed germination and the changes on phenolic profile has not been examined. In this study, we evaluated the transformation of phenolic compounds during seed germination produced by the inoculation with native PGPR on pepper seeds germination. Five bacterial strains: Cellulosimicrobium 60I1, Ochrobactrum 53F, Enterobacter 64S1, Pseudomonas 42P4 and Azospirillum brasilense Az39 were investigated. Our results showed that seed inoculation enhanced significantly seed germination, seedling growth, root length, and diameter of pepper seedling. In addition, changes in the profile of free and bound phenolic compounds were observed in seeds inoculated with PGPR. The free phenolic compounds were higher in the seedling inoculated with 60I1, 42P4 or the consortium with respect to control. The phenolic acid increased in seed inoculated suggests an elicitation of phenylpropanoid pathway related to ISR. The free flavonoids, naringenin, and rutin were present in the control treatment, while they were not detectable in the inoculated treatments. These could be used as a carbon source by PGPR during the germination or converted into flavonols that could protect the plants under abiotic stress. The tyrosol was present in 60I1 and in 60I1+42P4 treatment. The most effective strains were Cellulosimicrobium 60I1 and Pseudomonas 42P4 that increased their potential when it was used together.

Management of Source-Sink Balance for Maintaining Seed Vigor and Storability of Maize

Defoliation was the major factor which directly affected to maize seed yield and quality basis on source-sink relationship. Thus, the objectives were to study the effect of source-sink balance management on corn seed vigor and storability and to determine the effect of defoliation treatments on maize yield, yield components and produced seed germination traits. The experiment was arranged basis on Split-Plot in Randomized Complete Block Design with four replications. Main plot was the pattern of leave cutting with 5 levels: D1 = control, (without defoliating), D2 = complete defoliation, D3 = defoliating only under the ear, D4 = remain 2 top leaves, D5 = remain ear leaf. Sup-plot was leaf cutting dates which were C1 = 7 days after silking, C2 = 10 days after silking, C3 = 13 days after silking and C4 = 16 days after silking. Complete defoliation severely reduced ear weight, row number per ear, seed number per ear and 100 - seed weight (P<5%). Defoliation treatments had much more significantly affected on produced seed germination and seed vigor traits. Leaf defoliation intensity and leaf position affected total dry matter. Conclusion, the leaf defoliation only below ears was useful for source-sink balance management, because it promoted the seed qualities and vigor following to principle of parasitic sink elimination.

Sustainable improvement of seeds vigor using dry atmospheric plasma priming: Evidence through coating wettability, water uptake, and plasma reactive chemistry

Lentil seeds have been packed in a dielectric barrier device and exposed for several minutes to a cold atmospheric plasma generated in helium with/without a reactive gas (nitrogen or oxygen). While no impact is evidenced on germination rates (caping nearly at 100% with/without plasma exposure), seeds’ vigor is clearly improved with a median germination time decreasing from 1850 min (31 h) to 1500 min (26 h), hence representing a time saving of at least 5 h. We show that the admixture of nitrogen to helium can further increase this time saving up to 8 h. Contrarily, we demonstrate that the addition of molecular oxygen to the helium discharge does not promote seeds’ vigor. Whatever the plasma chemistry utilized, these biological effects are accompanied with strong hydrophilization of the seed coating (with a decrease in contact angles from 118° to 25°) as well as increased water absorption (water uptakes measured 8 h after imbibition are close to 50% for plasma-treated seeds instead of 37% for seeds from the control group). A follow-up of the seeds over a 45-days aging period shows the sustainability of the plasma-triggered biological effects: whatever the plasma treatment, seeds’ vigor remains stable and much higher than for seeds unexposed to plasma. For these reasons, the seed-packed dielectric barrier device supplied with a He–N2 gas mixture can be considered as a relevant dry atmospheric priming plasma in the same way as those used in routine by seed companies.

Calcium in the production and quality of cauliflower seeds

There are researches with calcium to produce the cauliflower inflorescence, but for seeds production no studies were found, this element is fundamental for the fixation of the floral buds and in the production and quality of seeds. The objective was to evaluate the effect of foliar application of calcium on different phenological stages of cauliflower plants in seed production and quality. Eleven treatments were evaluated, varying the number (1 to 4) of calcium (6 g L-1 of calcium chloride) applications and the phenological stages (E1 = floral stem with 30 cm length, E2 = opening of the first flowers; E3 = formation of first siliques; E4 = 50% of silique formed), resulting: T1 = control without application of Ca; T2 = E1; T3 = E1 + E2; T4 = E1 + E2 + E3; T5 = E1 + E2 + E3 + E4; T6 = E2; T7 = E2 + E3; T8 = E2 + E3 + E4; T9 = E3; T10 = E3 + E4; T11 = E4. The results showed a higher seed production per plant in treatments T10 (E3 + E4) and T11 (E4) with 42 and 48 g per plant, and a higher number of seeds per plant, with 12,379 and 12,978, respectively, compared to the control (T1): 23 g and 5,725 seeds per plant. Calcium applications in different phenological stages did not influence the physiological quality (germination and vigor) of cauliflower seeds of cultivar 'Piracicaba Precoce'. Application of these treatments in other cultivars is promising for further studies.

OsGRETCHENHAGEN3-2 modulates rice seed storability via accumulation of abscisic acid and protective substances.

Seed storability largely determines the vigor of seeds during storage and is significant in agriculture and ecology. However, the underlying genetic basis remains unclear. In the present study, we report the cloning and characterization of the rice (Oryza sativa) indole-3-acetic acid (IAA)-amido synthetase gene GRETCHEN HAGEN3-2 (OsGH3-2) associated with seed storability. OsGH3-2 was identified by performing a genome-wide association study in rice germplasms with linkage mapping in chromosome substitution segment lines, contributing to the wide variation of seed viability in the populations after long periods of storage and artificial ageing. OsGH3-2 was dominantly expressed in the developing seeds and catalyzed IAA conjugation to amino acids, forming inactive auxin. Transgenic overexpression, knockout, and knockdown experiments demonstrated that OsGH3-2 affected seed storability by regulating the accumulation level of abscisic acid (ABA). Overexpression of OsGH3-2 significantly decreased seed storability, while knockout or knockdown of the gene enhanced seed storability compared with the wild-type. OsGH3-2 acted as a negative regulator of seed storability by modulating many genes related to the ABA pathway and probably subsequently late embryogenesis-abundant proteins at the transcription level. These findings shed light on the molecular mechanisms underlying seed storability and will facilitate the improvement of seed vigor by genomic breeding and gene-editing approaches in rice.

CSN improves seed vigor of aged sunflower seeds by regulating the fatty acid, glycometabolism, and abscisic acid metabolism

IntroductionSunflower seeds possess higher oil content than do cereal crop seeds. Storage of sunflower seeds is accompanied by loss of seed vigor and oxidation of storage and membrane lipids. ObjectivesThis study first reported that compound sodium nitrophenolate (CSN), a new plant growth modulator, improved the germination and seedling emergence of aged sunflower seeds. The present study provide a future reference as to the potential applications of CSN and the regulation mechanism of exogenous substances in increasing aged crop seed vigor. MethodsPhenotypic analysis was performed to investigate the effect of CSN on germination and seedling emergence from naturally- and artificially-aged sunflower seeds. The biochemical and enzyme activity analysis were conducted to test the CSN-induced effect on glycometabolism, fatty acid and abscisic acid metabolism. Meanwhile, gene expression analysis was carried out to detect the changes in the transcription level of sunflower seeds during early germination period after CSN treatment. ResultsCSN application significantly increased the germination rate and seedling emergence rate of sunflower seeds under natural and artificial aging. Biochemical analysis indicated that, CSN treatment significantly enhanced the sucrose and fructose contents in aged sunflower seeds during early germination period. Moreover, the contents of several different fatty acids in CSN-treated sunflower seeds also significantly increased. Enzyme activity analysis revealed that CSN treatment remarkably up-regulated the activities of several critical enzymes related to triacylglycerol hydrolysis. Consequently, the transcription levels of the above key enzymes-related synthetic genes were also significantly up-regulated in CSN treatment. Furthermore, CSN treatment significantly decreased abscisic acid (ABA) content through the regulation of the gene expressions and activities of metabolism related-enzymes. ConclusionTaken together, the contribution of CSN to the improvement of aged sunflower seed germination and seedling emergence might be closely related to the fatty acid, glycometabolism, and ABA metabolism.

The Rice Small Auxin-Up RNA Gene OsSAUR33 Regulates Seed Vigor via Sugar Pathway during Early Seed Germination.

Seed vigor affects seed germination and seedling emergence, and therefore is an important agronomic trait in rice. Small auxin-up RNAs (SAURs) function in a range of developmental processes, but their role in seed vigor remains unclear. Here, we observed that disruption of OsSAUR33 resulted in reduced germination rates and low seed uniformity in early germination. Expression of OsSAUR33 was higher in mature grains and early germinating seeds. RNA-seq analysis revealed that OsSAUR33 modulated seed vigor by affecting the mobilization of stored reserves during germination. Disruption of OsSAUR33 increased the soluble sugar content in dry mature grains and seeds during early germination. OsSAUR33 interacted with the sucrose non-fermenting-1-related protein kinase OsSnRK1A, a regulator of the sugar signaling pathway, which influences the expression of sugar signaling-related genes during germination. Disruption of OsSAUR33 increased sugar-sensitive phenotypes in early germination, suggesting OsSAUR33 likely affects seed vigor through the sugar pathway. One elite haplotype of OsSAUR33 associated with higher seed vigor was identified mainly in indica accessions. This study provides insight into the effects of OsSAUR33 on seed vigor in rice.

Biochemical Investigations on Vigour Enhancement in Fresh and Aged Seeds Upon Seed Priming in Cowpea [Vigna unguiculata (L.) Walp.

Background: Loss in seed quality that occurs from maturity in the field to storage, leads to seed deterioration. Storage of cowpea seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzyme and protein levels. Method: The present study was performed to verify the effects of cowpea [Vigna unguiculata (L.) Walp.] seed priming (GA3, ammonium molybdate, Ca Cl2, KBr, Mg (NO3)2, ZnSO4, Hydro priming and dry non primed) with fresh and accelerated aged seeds.Result: The deterioration was rectified to the extent possible by the technique of seed priming. SDS PAGE profiling indicated the differential expression of proteins with seed priming. Esterase and peroxidase enzyme which were completely lost as a result of ageing showed reappearance after priming. The band intensity as well as the number of proteins induced by seed priming increased over control. Priming also restored the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds. The present study concluded that priming with GA3 (100 ppm) and Ammonium Molybdate (10-3 M) for 24 hours in aged seeds of cowpea showed increase enzyme activity, restored almost entire protein profile and esterase and peroxidise isozyme profile as it allowed repair system to combat sub-cellular damage and activated synthesis of enzymes and proteins.

Physiological and Biochemical Characteristics of Two Soybean Cultivars with Different Seed Vigor During Seed Physiological Maturity

Background: The soybean seed’s physiological maturity (R7) period is an extraordinary period for the formation of seed vigor. However, how proteins and their related metabolic pathways in seed and leaf change during seed physiological maturity is still not fully understood. Methods: In the present study, using a pair of pre-harvest seed deterioration-sensitive and -resistant soybean cultivars Ningzhen No. 1 and Xiangdou No. 3, the changes were investigated through analyzing leaf, cotyledon and embryo at the levels of protein, ultrastructure, and physiology and biochemistry. Results: Soybean cultivars with stronger photosynthetic capacity in leaf, higher nutrients accumulation and protein biosynthesis in cotyledon, as well as stronger resistant-pathogen ability and cell stability in embryo during seed physiological maturity, would produce higher vitality seeds. Conclusion: Such a study allows us to further understand the changes at protein, ultrastructure, and physiology and biochemistry levels in developing seeds during the physiological maturity and provide a theoretical basis for cultivating soybean cultivars with higher seed vigor.

Using the Extract of “Saprolin” for Enhancing Grain Crops

The solution to the problems of enhancing the yield of grain crops in current agriculture development is not conceivable without applying new land processing technology and using mineral fertilizers. Simultaneously, to obtain environmentally friendly products in organic farming, it is required to use mineral organic fertilizers in large quantities. For these purposes, we developed manufacturing technology and proposed the extract of “Saprolin.” This extract, obtained from organogenic lake storage, is supposed to be used for pre-sowing seed treatment, contributing to providing the planting material with biologically active substances, macro- and microelements, and natural soil conservation. During the production of the extract from the obtained source material, solutions of different concentrations were prepared, and their features (viscosity, density, electrical conductivity, refractive index, and pH of the medium) were studied. Using the wheat seeds “Novosibirskaya-31” and barley “Astana-2000” as an example, under the influence of solutions of the obtained extract, the germination and vigor of seeds were determined. As it was proved in experiments, the obtained extract is a good germination stimulant for grain crop seeds. It has a greater effect on seed vigor. Nevertheless, seeds with high vigor are the most viable, give good yield, and provide high-quality products. The germination rate significantly affects plants’ survival, shows the speed of their growth, and characterizes the ability of seeds to germinate in the field evenly (good uniformity). The research determines the germination and vigor of seeds using processing solutions of different concentrations. The research shows that the obtained extract of “Saprolin” increases the germination of seeds of grain crops by 1%–9% and vigor by 12%–19%. The extract of “Saprolin” is classified as a herbal stimulant containing a water-soluble natural mineral substance for improving plant nutrition.

Effect of intermittent drying on the energy consumption and physiological quality of soybean seeds

Energy efficiency can be considered as one of the main components of sustainability. This study aimed to explore an alternative to reduce the energy consumption of drying process, besides to preserve the nutritional reserve of soybean seeds by applying intermittent drying. We dried soybean seeds in a fixed bed dryer and compared the conventional and intermittent operation results. Physiological quality of dried seeds was assessed in terms of germination, vigor, and fissure, in addition, the energy consumption of the operation was calculated. Results indicate a 46% reduction in energy consumption for intermittent operation in comparison to conventional drying for air temperature of 50°C and 42% for air temperature of 70°C. High temperature (70°C) caused a reduction in germination and vigor of seeds, accelerating deterioration, and compromising soybean quality. Practical applications Drying is essential to prolong the life of soybeans, reducing the typical postharvest moisture content. However, one of the main disadvantages of this technique is the high-energy consumption and the loss in the nutritional and physical-chemical quality of the final product compared to other methods of drying, especially at high drying temperatures. The global energy crisis and consumption of quality dry foods further challenge the development of innovative food drying techniques to deal with these difficulties. Intermittent drying is considered as one of the promising alternatives to improve energy efficiency and product quality without increasing the cost of the dryer. The results of this study show that the use of intermittent drying is an alternative that significantly contributes to reduce the energy demand of the process and contributes to the quality of dry soybeans in the field.

Chickpea seed vigor evaluated by computerized seedling analysis

Computerized seedling analysis is a promising tool for assessing seed vigor, providing reliable and less subjective results. The aim of this study was to define a method for evaluating the vigor of chickpea seeds through computerized image analysis, using the Seedling Analysis System (Sistema de Análise de Plântula - SAPL®) as a basis. Two chickpea cultivars were evaluated, consisting of five seed lots of cv. BRS Aleppo and four seed lots of cv. Cícero. Test were performed on the seeds of each cultivar for initial characterization of the physiological potential of the lots, and the results were compared with the determinations made via SAPL®. The results showed high correlations between the variables obtained from computerized analysis and from traditional vigor tests, especially for the close relationship between the cold test and low temperature germination test with the corrected vigor index (r &gt; 0.9) evaluated on the fourth day after the beginning of the test. The total length of seedlings, length of the primary root, and growth and corrected vigor indices obtained through computerized analysis of seedlings on the 4th day after sowing allowed separation of the lots regarding vigor. Computerized analysis of seedlings using SAPL® is effective in determining the vigor of chickpea seed lots.