Fifty nine Spanish landraces of common wheat belonging to differentagrotypes were evaluated with four morphological spike characters and 11 seedprotein loci in order to study the level of agreement between the agrotypeclassification and the morphological and biochemical variation of the wheats. Asa result of the work 22 new gliadin alleles were found and 12 of them werecatalogued. Multivariate and cluster analysis performed on the two sets of data(morphological plus biochemical and biochemical only) showed that there was aclear relationship between seed protein composition and the wheat classificationfor the agrotypes ‘Barbilla’, ‘Candeal’ and‘Negrete’. For the other groups, agrotype classification was notconsistent with the variation for biochemical characters although, some allelesin common were found except for the ‘Jeja’ type. Morphologicaltraits revealed that they could be useful as first step in discriminating amongagrotypes. In contrast, biochemical markers showed that genetic variation amongaccessions was higher than among agrotypes. The protein lociGli-B1,Gli-A2,Gli-D2 andGli-A1 were shown to be the mostpolymorphic and useful in discriminating between the accessions studied.