Information security compliance means that library staff follows the rules, guidelines, and principles related to information security and implements the security measures provided in the library. This ensures the protection of sensitive information stored and processed in the library environment. This study examined the impact of information security awareness in academic libraries on information security compliance. In a descriptive survey design adopted in the study, the survey data of 136 personnel working in academic libraries in Türkiye were analysed. The findings revealed that information security awareness has a positive and significant impact on information security compliance (R2 = 0.983, β = 0.991, t(136) = 19.267, p < 0.05). The study concluded that information security awareness is necessary to ensure information security compliance in academic libraries. Consequently, there is a clear need for policy and training programs to enhance information security awareness and compliance among academic library staff. The findings of this study will not only contribute to the literature in the field of information security but also provide an important foundation for improving practices in this area. Therefore, it is important for academic libraries to develop effective policies and training programs to increase awareness of information security and promote staff compliance.