Aquaculture Sector Research Articles

Evolution through intellectual property rights in the aquaculture sector: reshaping aquaculture production networks

Evolution through intellectual property rights in the aquaculture sector: reshaping aquaculture production networks

Opportunities and risks of the blue economy for innovative companies in the sustainable aquaculture sector

The oceans play a crucial role in the global ecosystem and offer solutions to meet the growing demand for food, water, and energy. However, a new economic concept is needed to regulate and sustainably exploit the maritime resources offered by the marine ecosystem to ensure sustainable development. It is in this initiative that the concept of the blue economy has emerged, in response to the inadequacy of measures taken by States to meet their obligations and achieve their objectives in terms of marine biodiversity conservation. This is how the blue economy concept was born, in response to the inadequacy of state measures to conserve marine biodiversity. This concept proposes an innovative solution integrating aquatic and human resources into sustainable economic activities. The aim of this study is to understand the opportunities and threats that the blue economy represents for innovative companies. This short literature review, based on a SWOT (Strengths, Weaknesses, Opportunities, and Threats) diagnosis of innovative companies, identified a number of obstacles to the development of the blue economy, such as climate change, lack of financing and the lack of instruments for measuring the performance of the blue economy. Action strategies could be implemented to mitigate risks, threats and hazards, and consider an economic approach that reconciles marine and terrestrial ecosystems.

Antimicrobial use, residues and resistance in fish production in Africa: systematic review and meta-analysis

In low- and middle-income countries, data on antimicrobial use (AMU) and antimicrobial resistance (AMR) in aquaculture are scarce. Therefore, summarizing documented data on AMU, antimicrobial residue (AR), and AMR in aquaculture in Africa is key to understanding the risk to public health. Google Scholar, PubMed, African Journals online, and Medline were searched for articles published in English and French following the PRISMA guidelines. A structured search string was used with strict inclusion and exclusion criteria to retrieve and screen the articles. The pooled prevalence and 95% confidence intervals were calculated for each pathogen–antimicrobial pair using random effects models. Among the 113 full-text articles reviewed, 41 met the eligibility criteria. The majority of the articles reported AMR (35; 85.4%), while a few were on AMU (3; 7.3%) and AR (3; 7.3%) in fish. The articles originated from West Africa (23; 56.1%), North Africa (8; 19.7%), and East Africa (7; 17.1%). Concerning the antimicrobial agents used in fish farming, tetracycline was the most common antimicrobial class used, which justified the high prevalence of residues (up to 56.7%) observed in fish. For AMR, a total of 69 antimicrobial agents were tested against 24 types of bacteria isolated. Bacteria were resistant to all classes of antimicrobial agents and exhibited high levels of multidrug resistance. Escherichia coli, Salmonella spp., and Staphylococcus spp. were reported in 16, 10, and 8 studies, respectively, with multidrug resistance rates of 43.1% [95% CI (32.0–55.0)], 40.3% [95% CI (24.1–58.1)] and 31.3% [95% CI (17.5–49.4)], respectively. This review highlights the high multidrug resistance rate of bacteria from aquaculture to commonly used antimicrobial agents, such as tetracycline, ampicillin, cotrimoxazole, gentamicin, and amoxicillin, in Africa. These findings also highlighted the lack of data on AMU and residue in the aquaculture sector, and additional efforts should be made to fill these gaps and mitigate the burden of AMR on public health in Africa.

Strategy for Implementing the Blue Economy Concept for Community Empowerment in Achieving Sustainable Development Goals

Indonesia has around 17,500 islands spread throughout the archipelago and a coastline that stretches for 81,000 km, more than half of Indonesia's total area is sea and water. This geographical condition makes Indonesia a country that has rich marine resources. However, challenges such as illegal fishing and lack of empowerment of coastal communities threaten the sustainability of the fisheries sector. Implementing the Blue Economy concept is the key to achieving Sustainable Development Goals (SDGs). Through a literature study, this article investigates strategies for implementing the Blue Economy in empowering communities in Indonesia to achieve the SDGs, especially goal 14: Life Below Water. Strategic steps are needed such as increasing supervision, developing technology and building infrastructure to improve community welfare and maintain the sustainability of marine resources. With great potential in the aquaculture sector, Indonesia can realize the vision of sustainable development through collaboration between various parties and the application of innovative and sustainable Blue Economy principles.

Development of microbound diet and its application on Macrobrachium rosenbergii larvae and postlarvae

The aquaculture sector actively seeks innovative methods to enhance growth and nutrition in aquatic organisms. Microbound diets (MBDs), known for their finely ground pellets often combined with binders to encapsulate nutrients are gaining prominence. This study aimed to develop an MBD and assess its impact on the growth and body composition of giant freshwater prawn, Macrobrachium rosenbergii larvae and postlarvae (PL) through two feeding trials. Various binders (agar, zein, alginate, carrageenan, and carboxymethyl cellulose) were tested during the development of MBD and were compared with live feed (rotifer). The first feeding trial demonstrated improved larval growth with MBD with agar, reaching an average of Stage XII, compared to those fed the control diet, which only reached Stage IX. The second feeding trial investigated the effect of feeding regimes on giant freshwater prawn PL, consisting of (i) one-time feeding in the morning, (ii) one-time feeding at night, (iii) two-time feeding, (iv) three-time feeding, and (v) four-time feeding. Results showed that two-time feeding had comparable survival rates and growth performance to three-time feeding (survival rate= 43–45 % and weight gain= 0.09–0.11 g), with the best feed conversion ratio in two-time feeding (1.70) and the highest level of highly unsaturated fatty acids (25–26 %) in PL fed one-time and three-time. Overall results suggest that MBD with agar holds promise as a binder with two-time and three-time feeding, enhancing the quality of giant freshwater prawn PL. Further research on extending the duration of the experiment is recommended to assess PL moulting patterns and performance, as varied feeding regimes may significantly impact growth and moulting.

Navigating aquaculture losses: Tackling fish‐killing phytoflagellates in a changing global landscape

AbstractAquaculture has emerged as a pivotal alternative for seafood production, addressing the challenges posed by depleting wild fish stocks. However, the industry faces substantial challenges, notably the presence of harmful algal blooms (HABs), which can cause serious economic consequences. The proliferation of major species of phytoflagellates leading to massive fish mortality is on the rise, possibly attributed to increasing global temperatures and eutrophication. This underscores the critical need for robust detection, prevention, and mitigation strategies to safeguard the aquaculture industry. This contribution offers valuable insights into comprehending and addressing the challenges posed by HABs in the fish aquaculture sector in an era of global change.

Changes in blood serum parameters in farmed rainbow trout (Oncorhynchus mykiss) during a piscine lactococcosis outbreak.

The aquaculture sector plays a vital role in global food security, yet it grapples with significant challenges posed by infectious diseases. Piscine lactococcosis is one of the significant threats in rainbow trout aquaculture due to its potential to cause severe economic losses through mortalities, reduced growth rates, and increased susceptibility to other pathogens. It poses challenges in disease management strategies, impacting the sustainability and profitability of rainbow trout farming. The current study focuses on the variations in serum blood parameters of farmed rainbow trout Oncorhynchus mykiss during a lactococcosis outbreak caused by Lactococcus garvieae. Blood samples were collected for biochemical analysis, fish were examined for parasites and bacteria, and DNA from bacterial colonies was PCR-amplified and sequenced for identification. Overall, 13 biochemical parameters, including proteins, enzymes, lipids, chemicals, and minerals, were measured in serum blood samples from both diseased and healthy fish. The results indicate significant alterations in the levels of these parameters during the outbreak, highlighting the impact of infections on the blood profile of farmed rainbow trout. Urea levels were significantly higher in diseased fish compared to controls, and creatinine, phosphorus, and magnesium also showed similar trends. Alanine aminotransferase and total protein levels were higher in control fish. Chloride levels differed significantly between groups. Iron levels were higher in controls and lower in diseased fish. No significant differences were found in other parameters. This study reveals significant changes in serum blood parameters of rainbow trout during a lactococcosis outbreak caused by L. garvieae. These changes highlight the potential of these parameters as tools for monitoring health status, stress, and aquaculture management. Continuous monitoring can provide valuable insights into disease severity and overall fish health, aiding in the development of improved management practices. The presented data contribute to understanding the pathophysiology of piscine lactococcosis and developing effective mitigation strategies for farmed rainbow trout.

Web‐based application to guide sampling on salmon farms for more precise estimation of sea louse abundance

AbstractObjectiveEfficiently managing sea lice on salmon farms through active surveillance, crucial for lice abundance estimation, is challenging due to the need for effective sampling schemes. To address this, we developed an application that considers infestation levels, farm structure, and management protocols, enhancing the precision of sampling strategies for sea louse abundance estimation.MethodsSimulation‐based methods are valuable for estimating suitable sample sizes in complex studies where standard formulae are inadequate. We introduce FishSampling, an open Web‐based application tailored to determine precise sample sizes for specific scenarios and objectives.ResultThe model incorporates factors such as sea lice abundance, farm pen numbers, potential clustering effects among these pens, and the desired confidence level. Simulation outcomes within this application provide practical advice on how to decide on the number of fish and pens to sample, under varying levels of assumed clustering.ConclusionThis approach can be used across the salmon aquaculture sector to improve sampling strategies for sea lice abundance estimation and balance surveillance costs against health objectives.

Transforming Aquaculture through Vaccination: A Review on Recent Developments and Milestones.

Aquaculture has rapidly emerged as one of the fastest growing industries, expanding both on global and on national fronts. With the ever-increasing demand for proteins with a high biological value, the aquaculture industry has established itself as one of the most efficient forms of animal production, proving to be a vital component of global food production by supplying nearly half of aquatic food products intended for human consumption. As in classic animal production, the prevention of diseases constitutes an enduring challenge associated with severe economic and environmental repercussions. Nevertheless, remarkable strides in the development of aquaculture vaccines have been recently witnessed, offering sustainable solutions to persistent health-related issues challenging resilient aquaculture production. These advancements are characterized by breakthroughs in increased species-specific precision, improved vaccine-delivery systems, and innovations in vaccine development, following the recent advent of nanotechnology, biotechnology, and artificial intelligence in the -omics era. The objective of this paper was to assess recent developments and milestones revolving around aquaculture vaccinology and provide an updated overview of strengths, weaknesses, opportunities, and threats of the sector, by incorporating and comparatively discussing various diffuse advances that span across a wide range of topics, including emerging vaccine technologies, innovative delivery methods, insights on novel adjuvants, and parasite vaccine development for the aquaculture sector.

Sensing Technologies and Automation: Revolutionizing Aquaculture toward Sustainability and Resilience

Given the aquaculture industry’s role at the forefront of addressing food security challenges facing the growing global population, the aquaculture sector also encounters various constraints that can impact its productivity, sustainability and resilience. These constraints including environmental issues, diseases, high reliance on the workforce and competition for water resources. This review provides an overview of the roles of sensing and automation technologies in revolutionizing aquaculture toward sustainability and resilience. Innovations in sensing and automation stand out as among the critical game-changing advancements that are transforming the way aquaculture industry operates. Intelligent sensing technologies offer major advantages in monitoring the environment, animal behavior and growth, as well as early detection of diseases. By integrating advanced smart sensing devices, internet of things (IoT), artificial intelligence (AI) and automated response devices, aquaculture operations can be observed and managed remotely in real-time, leading to enhanced productivity, efficiency, resource utilization, environmental friendliness, reduced workforce dependency and competition over water. Through these revolutionary innovations, the aquaculture industry fosters a more sustainable and resilient future while contributing toward food security. Collaborative engagement of all stakeholders is crucial in realizing the full potential of sensing and automation innovations in aquaculture.

A Case Study on Economic Analysis of Sea Cage Farms in the Black Sea, Türkiye

The main aim of this research is to understand the value chains of enterprises engaged in aquaculture in sea cages in the Black Sea region of Türkiye, to determine production costs and to make a general analysis of the aquaculture sector. Random sampling method was used to ensure that farmers were equally represented in the survey. Data for the survey were randomly selected from 16 separate farms spread throughout 4 different marine cage locations. Furthermore, fish traders and sector stakeholders were interviewed. The businesses examined within the scope of the study are divided into 3 groups: small (250 -500 tons/year), medium (501-999 tons/year) and large (>1000 tons/year), according to their capacities. The most important expense items of enterprises consist of variable costs. Among the variable costs, the purchase of fry fish, feed purchase, and labor costs constitute the most important expenditure items. Among the fixed costs, the most important expense is depreciation costs. The unit cost of one kg of fish varies between 2.7 and 3.0 euros. Considering that the trout (Oncorhynchus mykiss) and seabass (Dicentrarchus labrax) in the Black Sea are at the level of 3.5 Euro, the profit rate of the enterprises is between these levels. Recently, since trout from the Black Sea has been exported to the Far East Country (particularly Japan), its price has also increased. In 2018, the average retail price (including VAT) and medium selling price of seabass and trout were at a level of 5.24 and 5.34 Euros respectively. Despite all these positive developments, climate change continues to pose a threat in terms of disease outbreak and marketing problems.

Effects of dietary astaxanthin on immune status and lipid metabolism in gilthead seabream (Sparus aurata)

Astaxanthin (AX) is a carotenoid known to have one of the highest documented antioxidant capacities and has attracted considerable scientific and commercial interest. The incorporation of AX into aquaculture practices has been associated with improved pigmentation, modulation of the immune and endocrine systems, stress reduction, reproductive efficiency and general fish health. This study describes the effects of dietary AX (0, control, 20, 100 and 500 mg kg−1 AX per kg of diet) for 15 and 30 days on growth performance, immune and antioxidant status, histology and gene expression in gilthead seabream (Sparus aurata). Fish fed diets enriched with 500 mg kg−1 of AX for 15 days decreased in skin mucus peroxidase activity while at 30 days of trial, fish fed a diet supplemented with 20 mg kg−1 AX increased the peroxidase activity in serum. In addition, bactericidal activity against Vibrio harveyi increased in the skin mucus of fish fed any of the AX supplemented diets. Regarding antioxidant activities in the liver, catalase and glutathione reductase were decreased and increased, respectively, in fish fed a diet supplemented with 500 mg kg−1 of AX. Finally, although the expression of up to 21 inflammatory and lipid metabolism-related genes was analysed in visceral adipose tissue, only the expression of the interleukin 6 (il6) gene was up-regulated in fish fed a diet supplemented with 20 mg kg−1 of AX. The present results provide a detailed insight into the potent antioxidant properties of AX and its possible modulatory effects on the immune status and lipid metabolism of seabream, which may be of interest to the aquaculture sector.

Can farmed fish match the preference of consumers accustomed to wild fish? Evidence from Central Malawi

The existing capacity of capture fisheries in Malawi is operating at its maximum potential, unable to meet the growing demand for fish. To address this issue, expanding the output of the aquaculture sector appears to be the only viable solution for augmenting fish supply and consumption. However, consumers in Malawi prefer fish sourced from their natural habitats, often considering farmed fish as inferior. Fortunately, advancements in breeding techniques and aquaculture management have led to improved quality of farmed fish. In this study, our objective was to identify and compare the desired characteristics and marketing preferences of fresh tilapia among consumers in Malawi with those of aquaculture-produced fish. This comparison aimed to determine if farmed fish align with consumer expectations. A conjoint experiment was conducted in Lilongwe city targeting 280 fresh tilapia fish consumers. Preferences and associated willingness to pay were modeled using rank-ordered logit regression. The findings indicate that consumers favor smaller-sized fish (around 130 g), a size that is currently achievable through aquaculture practices. Interestingly, consumers displayed less enthusiasm for a pricing model based solely on weight. Notably, although consumers maintain a belief that wild-caught fish is superior to farmed fish, they indicated a willingness to pay for farmed products. The current technology and practices allow aquaculture farms to produce fish within the preferred size range, which presents an opportunity for sector expansion. However, achieving this potential requires effective marketing strategies to shift cultural perceptions that currently hinder the acceptance of farmed fish.

Toward the Circular Economy in the Aquaculture Sector: Bibliometric, Network and Content Analyses

This paper offers an overview of circular economy strategies applied to the aquaculture sector. The growing challenges imposed on the sector by the strategies of the Green Deal impose new growth strategies in the name of sustainability. The scalability of these strategies is increasingly hampered by regulatory voids and by the absence of a universally accepted assessment method for measuring the impacts of current aquaculture systems. More than ever, a review of knowledge in the circular economy field is required to comprehend where the aquaculture sector is heading, and in order to make the required transition. The present review proposes a bibliometric analysis, a network analysis and a content analysis, which highlight a very new and expanding field of research. The studies were firstly analyzed from a micro (animal metabolism) to a macro perspective (policies, markets and society), emphasizing where research is still lacking. Furthermore, a second level of classification concerns the type of circularity approach proposed for the aquaculture system, which can be divided into open-loop or closed-loop strategies. Regarding the open-loop-related studies, the focus of the evaluation is devoted to the different bioeconomic values of the circularity strategies proposed for the biological flows entering and exiting the aquaculture system. The literature review offered insights into the identification of research threads that are developing around the aquaculture sector.

Evolution and Prospects of Freshwater Fish Species and Aquaculture in Colombia: A Historical Perspective

Since the 1970s Colombia has been aware of the importance of aquaculture and inland fisheries in the future of its economy due to the constant increase of a population that is increasingly in need of protein of animal origin. In 1972, a joint project between the Government of Colombia and the Food and Agriculture Organization of the United Nations was approved and aimed at studying inland fishing and developing aquaculture in the country. The work of the project is described, as are the fish culture and fisheries research activities starting with the situation in the 1970s but comparing it with recent findings. A list of the main capture and culture fish species is given. Colombian fishing production has had great variations, but first inland water fishing was more important than marine. For instance, in 1972 total fish catch was 104,390 tons and 79% of that originated from inland waters. After the continental fisheries went down, the marine fisheries accounted in 2019 already 78 per cent of the total catch of the country. Mean standing fish crop estimates in 1975‒76 from the bay, open water and vegetation habitats in four floodplain lakes were 118, 12 and 251 kg/ha indicating clearly that the fish productivity on the Magdalena floodplain was relatively low. The aquaculture sector has been growing rapidly, reflecting global trends, and producing more than capture fisheries. Aquaculture produced 88,000 tons in 2013, which was a three-fold increase from the mid-1990s. Again twofold increase took place before 2020 when the production was almost 180,000 tons. Future development prospects of inland fisheries and aquaculture are discussed and a mention is made of the present situation of ornamental fish cultivation and trade in Colombia.

Fisheries and aquaculture by-products: Case studies in Norway, United States, and Vietnam

Fishmeal and fish oil (FMFO) have historically come from capture fisheries, although supply from capture fisheries is constrained and demand for FMFO is increasing. Fish production losses and by-products are an alternative raw material for FMFO, however, there is little systematic data collected on the use of these raw materials and limited knowledge about barriers to their use in FMFO production. This study collected data on production losses and by-products from seven fisheries and aquaculture sectors that are important in supplying the US seafood market. From 2019–2021, quantitative surveys (n=47 businesses), semi-structured qualitative interviews (n=31 businesses), and secondary data were collected for the study period of 2014–2018. There was significant variation in utilization of production losses and by-products across sectors (range: 37–99 %), and overall, the aggregate utilization rate was 72 %. Scale appeared to be the most important factor leading to use of production losses and by-products. Aquaculture industries in this study had a large and relatively steady supply of by-products year-round, which made investments in FMFO plants worthwhile and led to moderate to high rates of by-product utilization. Wild-caught fisheries in this study had lower rendering rates due to short fishing seasons, smaller scales, operations in remote locations, and regulations that allow dumping of by-products. There were several examples of companies that invested in rendering plants because it was profitable to sell the rendered waste, while other sectors and industries require better coordination and policy supports to make use of this valuable resource.

Unveiling the molecular regulatory mechanisms of immune responses in the spleen of spotted sea bass (Lateolabrax maculatus) against Nocardia seriolae infection

Spotted sea bass holds significant economic importance in China's aquaculture sector. The outbreaks of bacterial diseases, especially caused by Nocardia seriolae (N.seriolae), have occurred frequently due to intensive farming. With the high morbidity and mortality, fish nocardiosis is one of the most serious problems faced by the aquaculture industry of spotted sea bass. However, the regulatory mechanism of immune response in spotted sea bass after N.seriolae infection remain elusive. In our study, we conducted a comprehensive examination of clinical symptoms and histological structures of the spleen, revealing noticeable pathological changes in diseased fish. Subsequently, we employed transcriptome sequencing of the spleen to investigate the effect of N.seriolae on spotted sea bass at 0 h, 48 h, 96 h and 120 h at transcriptome level. A total of 3031, 3597, and 4683 differentially expressed genes (DEGs) were unveiled in 48 h vs. 0 h, 96 h vs. 0 h and 120 h vs. 0 h, respectively. Up-regulated DEGs were significantly enriched in PRRs and cytokine related pathways, while down-regulated DEGs exhibited significant enrichment in autophagy related pathways, suggesting potential roles in immune modulation. Weighted co-expression network analysis (WGCNA) identified a blue module strongly linked to the immune response against N.seriolae infection, from which 10 hub genes were identified, including ilr2, tnfrsf9, c7 and so on. Moreover, our findings highlighted the involvement of alternative splicing (AS) in the immune response and found that pathogen infection also induced the AS events in some immune-related genes. Our study could shed new light on the molecular regulatory mechanisms underlying the immune response after N. seriolae infection in spotted sea bass, providing a basic guidance for the breeding of spotted sea bass with disease resistance in the future.

Comparative Nutritional and Histological Analysis of Malabar Red Snapper (Lutjanus malabaricus) and Asian Seabass (Lates calcarifer).

This study offers a comprehensive morpho-histological analysis of the gastrointestinal tract (GIT) of the Malabar red snapper. A comparison of its GIT morphology with that of the Asian seabass reveals similarities and differences between the two species. Additionally, the moisture content, crude protein, and ash in the fillets of Malabar red snapper and Asian seabass were slightly different, with Malabar red snapper exhibiting higher levels of essential fatty acids. Furthermore, higher levels of the polyunsaturated fatty acid (PUFA)/saturated fatty acid (SFA) ratio and docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) ratio, and a lower omega-6/omega-3 ratio, were observed in Malabar red snapper compared to Asian seabass. The Malabar red snapper's esophagus featured protective mechanisms such as simple columnar epithelial cells, mucous-secreting glands, and goblet cells that were predominantly stained for acid and neutral mucosubstances. Furthermore, its stomach, with mucus cells that were weakly stained for acid mucosubstances, exhibited distinct regions with varying glandular densities, with the pyloric region featuring few glands. The pyloric caeca of the fish were composed of five finger-like structures and few goblet cells. Several goblet cells gradually increased from the anterior to the posterior region of the intestine. These findings provide useful insights for the aquaculture sector, focusing on Malabar red snapper.

Immunological effects of DNA vaccination and interleukin utilization as an adjuvant in Astyanax lacustris immunized against Ichthyophthirius multifiliis.

The increasing significance of the aquaculture sector and commercially valuable species underscores the need to develop alternatives for controlling diseases such as Ichthyophthirius multifiliis-induced ichthyophthiriasis. This ciliated protozoan parasite threatensnearly all freshwater fish species, causing substantial losses in the fishery industry. Despite this, effective large-scale treatments are lacking, emphasizing the necessity of adopting preventive strategies. While the pathogenesis of ichthyophthiriasis and its immune stimulation allows for vaccination strategies, precise adjustments are crucial to ensure the production of an effective vaccine compound. Therefore, this study aimed to evaluate the impact of immunizing Astyanax lacustris with a genetic vaccine containing IAG52A from I. multifiliis and the molecular adjuvant IL-8 from A. lacustris. Transcript analysis in immunized A. lacustris indicated mRNA production in fish muscles, demonstrating an expression of this mRNA. Fish were divided into five groups, receiving different vaccine formulations, and all groups received a booster dose 14 days after the initial immunization. Samples from vaccinated fish showed increased IL-1β mRNA expression in the spleen within 6 h post the second dose and after 14 days. In the head kidney, IL-1β mRNA expression showed no significant difference at 6 and 24 h but an increase was noted in fish injected with IAG and IAG + IL-8 after 14 days. IL-8 mRNA expression in the spleen and kidney did not significantly differ from the control group. Histological analysis revealed no variation in leukocyte concentration at 6 and 24 h post-vaccination; however, after 14 days, the groups injected with IAG and IAG + IL-8 exhibited a higher leukocyte density at the application sites thanthe control. The obtained data suggest that the used vaccine is transcribed, indicating its potential to stimulate innate immune response parameters through mRNA cytokine expression and leukocyte migration.

Investigation on parasitic infestation of Labeo rohita (Hamilton, 1822) in the south-western region of Bangladesh.

Our investigation focused into Labeo rohita, commonly known as Rui, a freshwateraquatic species in Bangladesh. Despite their nutritional significance, these fish faced a pressing challenge: parasite infections threaten the economic stability of the aquaculture sector. The present study aimed to investigate the parasite and histological changes in major organs of L. rohita, collected fromKhulna region - Dumuria, Paikgacha and Rupsha. About 180 (30/month) specimens were collected between the month of March and August 2023 to observe the parasitic status in L. rohita. Through microscopic examination, a total of 323 parasites were uncovered, spanning categories including Cestode, Nematode, Acanthocephala, Trematode and Digenia, predominantly residing in the intestines of L. rohita. The highest prevalence rate (70%) was recorded in both March and May 2023, with peak mean intensity observed in July (3.73). Notably, the highest mean abundance (2.37) exhibited in July and index of infestation (45.34) in June. Histological analysis confirmed parasitic infestations in the gastrointestinal region, with displaying histological changes within major organs such as the liver, kidney, gills, spleen and testicles due to parasitic infection. This study concluded that the indentified six categories of parasite and the affect of parasitic infestation in major organs of L. rohita within the study period. Urgent efforts to implement effective strategies for controlling the parasite infections in aquaculture to ensure the sustainable production of this invaluable fish species.