Sectioning Technique Research Articles

Ion Mobility and Fourier Transform Ion Cyclotron Resonance Collision Cross Section Techniques Yield Long-Range and Hard-Sphere Results, Respectively.

We determined collision cross section (CCS) values for singly and doubly charged cucurbit[n]uril (n = 5-7), decamethylcucurbit[5]uril, and cyclohexanocucurbit[5]uril complexes of alkali metal cations (Li+-Cs+). These hosts are relatively rigid. CCS values calculated using the projection approximation (PA) for computationally modeled structures of a given host are nearly identical for +1 and +2 complexes, with weak metal ion dependence, whereas trajectory method (TM) calculations of CCS for the same structures consistently yield values 7-10% larger for the +2 complexes than for the corresponding +1 complexes and little metal ion dependence. Experimentally, we measured relative CCS values in SF6 for pairs of +1 and +2 complexes of the cucurbituril hosts using the cross-sectional areas by Fourier transform ion cyclotron resonance ("CRAFTI") method. At center-of-mass collision energies <∼30 eV, CRAFTI CCS values are sensitive to the relative binding energies in the +1 and +2 complexes, but at collision energies >∼40 eV (sufficient that ion decoherence occurs on essentially every collision) that dependence is not evident. Consistent with the PA calculations, these experiments found that the +2 complex ions have CCS values ranging between 94 and 105% of those of their +1 counterparts (increasing with metal ion size). In contrast, but consistent with the TM CCS calculations, ion mobility measurements of the same complexes at close to thermal energies in much less polarizable N2 find the CCS of +2 complexes to be in all cases 9-12% larger than those of the corresponding +1 complexes, with little metal ion dependence.

Gambaran Caffeine Addiction Pada Ibu Rumah Tangga

Caffeine is still a popular processed food or drink among teenagers and adults, as well as housewives who still have the habit of consuming caffeine such as coffee or tea. Caffeine as an antagonist at the adenosine receptor will stimulate dopaminergic activity which will cause psychostimulant properties, namely providing a wakeful and arousing effect which can then lead to potential addiction or addiction to caffeine. Consuming caffeine in excess of the daily limit can cause side effects including anxiety, insomnia, increased heart rate, and gastrointestinal disturbances. Therefore, the purpose of this study was to determine the description of caffeine addiction in housewives. The research method using descriptive analysis with sampling using cross sectional technique obtained a sample of 33 people. The data was obtained from filling out questionnaires by respondents about caffeine addiction, then the questionnaire was tested for validity and reliability. The results obtained from the validity test of 9 questionnaire questions, namely r count &gt; r table (0.344), it can be concluded that the questionnaire is valid. Furthermore, the reliability test on the questionnaire obtained the results of the Cronbach alpha value of 0.933 which means &gt; 0.6 so it can be concluded that the questionnaire in this study is said to be reliable. To find out the description of caffeine addiction in respondents, univariate analysis with SPSS showed that most respondents experienced caffeine addiction in the moderate category, namely 29 respondents (87.9%), and 4 respondents (12.1%) experienced caffeine addiction in mild category. The conclusion of this study is that housewives experience caffeine addiction in the moderate category, namely 29 respondents. The importance of limiting daily caffeine intake to minimize the risk of addiction to caffeine and the side effects caused by caffeine.&#x0D;

Analysis between cognitive impairment with the level of disability post-stroke patients: A cross-sectional study

Introduction: The condition that appears in patients who have had a stroke is cognitive impairment. Cognitive impairment can worsen the condition of stroke patients so that it can slow down the recovery process and have a high risk of disability. This study aims to analyze the correlation between cognitive impairment and the level of disability in post-stroke patients. Methods: This study uses a correlational analytical design with a cross sectional technique design with a population of 70 participants and a sampling technique using consecutive sampling obtained a sample of 64 participants. The independent variable in this study is cognitive impairment of post-stroke patients while the dependent variable is the level of disability of post-stroke patients. The instrument for collecting data on cognitive impairment was using the MoCA-Ina questionnaire and the level of disability using the WHODAS questionnaire. The data were analyzed using the Spearman Rank test with significance &lt; 0.05. Results: The results of this study showed that most of the 52 (81.2%) participants had cognitive impairment and most of the 42 (65.6%) participants had moderate disabilities and a small portion of 10 (15.6%) participants had severe disabilities. The results of statistical tests on the relationship between cognitive impairment and the level of disability in post-stroke patients through the Spearman Rank test showed p = 0.000 with coefficient correlation 0.665. Conclusions: The analysis test showed a strong correlation between cognitive impairment and the level of disability in post-stroke patients. Post-stroke patients with cognitive impairment must be treated properly so that it does not result in disability in patients that has a direct impact on the psychosocial and quality of life of the patient.

Mechanical property and structure of polycarbonate /aluminum alloy hybrid prepared by ultrasound-assisted hot-pressing technology

ABSTRACT Polycarbonate/aluminum alloy hybrids were prepared using a homemade ultrasound-assisted hot pressing molding (UAHPM) technique, and their properties and structures at the plastic-metal interface were also investigated. The aluminum alloy surface was chemically etched and anodized to form micron and nano-holes structures. The joint strength of 21.58 MPa was achieved at the optimum molding process by optimizing the process parameters of ultrasonic hot compression molding. The specific structure and morphology of the polycarbonate/aluminum hybrid material bonding layer were observed by field emission scanning electron microscopy (FE-SEM) using the ultrathin sectioning technique, and the plastic embedding depth reached about 3.8 µm under the optimal process conditions. X-ray photoelectron spectroscopy (XPS) analysis revealed that the bonding layer not only has a micro-nano interlocking structure but also forms an Al-O-C chemical bonding interaction. The plastic in the bonding layer was exposed by dissolving the aluminum alloy of the joint, and the filling form of the plastic in the micro-nanoholes was directly observed.

Recurrence Rate of Abdominal Tuberculosis in Cases Presenting With Acute Abdomen

Background: Acute abdomen is considered as one of the major causes leading to surgical emergencies and intestinal obstruction, perforation and many other underlying causes resulting in surgical consultation. Aim: To determine rate of recurrence of abd. TB in cases presenting with acute abdomen to Sh. Zayed Hospital, Rahim Yar Khan. Study Design: The Cross sectional study Sampling Technique: Consecutive sampling non probability Sampling Technique Methods: This particular study was conducted at teaching hospital Rahim Yar Khan from 10-04-2021 to 31-12-2022 in which the cases of both genders with age ranging from 15 to 60 years having acute abdomen presenting within 24 hours were included. Abdominal tuberculosis (TB) was labeled on histopathology when there was caseating granuloma. Results: There were around 90 cases included in this present study. Out of these 50 (55.55%) were male patients and the rest of 40 (44.44%) were female patients. Approximately 34.91±11.35 was the Mean age in years of the patients and 67.13±21.39 in Kilograms was the mean weight. There were about 22 (24.44%) cases which had previous history of abdominal surgery. The abdominal tuberculosis was diagnosed in 30 (33.33%) of the cases as shown in figure 12. Abdominal TB was observed in 16 (32%) males and 14 (35%) females in their respective groups with p= 0.81. TB was detected in 20 (35.71%) out of 56 cases in age group 15 to 39 years as compared to 6 (25%) out of 24 cases with age 40-60 years with p= 0.44. There was no significant difference in terms of abdominal TB and weight (p= 1.0) and also with cases that had previous surgery with p= 0.57. Conclusion: The abdominal tuberculosis (TB) is detected in one third of all the cases presenting with acute abdomen and there is no significant association of this with any of the confounding variable of the study. Key words: Acute abdomen, abdominal TB, Granuloma, Biopsy

Comparison of Pfannenstiel &amp; Joel Cohen Cesarean Section Technique in Pregnant Women Referred to Mahzad Hospital

Introduction: Cesarean delivery can lead to higher costs and complications such as infection, bleeding, anemia, and the possibility of blood transfusion, hysterectomy, and infertility compared to normal delivery. Therefore, in this study, we want to comparison the incidence of complications in cesarean section Consider the Joel-Cohen and Pfannenstiel methods. Materials and Methods: In this comparative study, 100 patients participated. All patients were randomly operated on by Pfannenstiel (n = 50) and Joel-Cohen (n = 50) methods. Time to start surgery until the fetus delivery, Time to start surgery until the end of the surgery, Number of blood-stained gases, Need to pethidine, As an indicator of postoperative pain, temperature above 37.8°C as fever and urinary residual more than 100 cc after urinary catheter withdrawal and after the first urination with nelaton catheter as urinary obstruction, among all Patients were compared. Results: In this study. The mean age was 26.72±7.43 years old in the Pfannenstiel group and 21.44±6.51 years old in the JoelCohen group (P = 0.84). The mean body mass index (BMI) was 24.84 ± 2.48 Kg / m² in the Pfannenstiel group and 24.50 ±3.14 Kg / m² in the Joel-Cohen group (P = 0.55). The mean gestational age in the Pfannenstiel group was 38.74 ± 1.58 weeks and in the Joel-Cohen group was 38.82 ± 1.58 weeks (P = 0.80). Fever and urinary obstruction were not seen in any of the patients. The mean duration of surgery, fetus delivery time, bleeding rate, length of hospital admission, and pain intensity after surgery was significantly higher in patients who underwent Pfannenstiel cesarean section, compared to the Joel-Cohen group. Conclusion: Joel-Cohen method is preferable to a method in the cesarean section.

Evaluation of Axillary Lymph Node Marking with Magseed® before and after Neoadjuvant Systemic Therapy in Breast Cancer Patients: MAGNET Study.

Background Due to the high false negative rate (FNR) associated with sentinel lymph node biopsy (SLNB) after neoadjuvant systemic therapy (NAST), the standard surgical treatment for patients with an initially positive axilla and indicated for NAST is axillary lymph node dissection (ALND). To avoid unnecessary ALND, this multicenter, prospective, observational study aimed to determine the effectiveness and ease of using magnetic seeds (Magseed®) for targeted axillary dissection (TAD) when the seeds are placed before or after NAST. Materials and Methods We recruited 81 patients diagnosed with T1-T3 breast cancer, with clinically/radiologically positive nodal involvement (cN1, 75 patients with 1–3 nodes suspected nodes and 6 patients with up to 4 suspected nodes) prior to NAST. Positive nodes detected by fine-needle aspiration biopsy or core needle biopsy were marked with a stainless steel marker coil and after NAST with Magseed® prior to surgery (Post-NAST group), or directly with Magseed® before NAST (Pre-NAST group). The correlation between lymph nodes marked with Magseed® (MLNs) and sentinel lymph nodes (SLNs) was calculated based on pathologic assessment with the OSNA assay (Sysmex Corporation, Kobe) or conventional sectioning and staining techniques according to the standard protocols of each center. Results All magnetic seeds were successfully identified and retrieved in just over 10 minutes of surgery, guided by the Sentimag® magnetometer system. The overall concordance rate between MLNs and SLNs was 81.5%, and the concordance between MLNs and SLNs with metastasis was 93.8%. Metastasis was detected in 54.3% of the MLNs compared with 48.1% of SLNs. In cases that presented negative MLN and negative SLN (negative TAD), the FNR was 0%. No significant differences were found between the Post-NAST and Pre-NAST groups. Conclusions Our results validate the use of Magseed® for long-term marking of axillary lymph nodes and show that when used in combination with SLNB for TAD, a FNR of 0% can be achieved, avoiding unnecessary ALND.

Heat transfer and mixing enhancement by Poiseuille-Taylor-Couette flow between two rotating elliptically-deformed annular tubes

Multifunctional heat exchangers (HEX) are very important devices which are used in many industrial applications (aerospace, aeronautics, automobile, process and chemical engineering, etc). Improving their designs for an optimal overall performance is still a wide window for both research and development. In the present contribution, a new HEX design is proposed as two concentric elliptically-deformed tubes of complex geometry. Employing advanced computational fluid dynamics (CFD), we show how a combined Poiseuille-Taylor-Couette (PTC) analogous flow between two rotating concentric elliptically-deformed annular tubes, can efficiently enhance heat and mass transfer at low to medium Reynolds numbers (Re≤488). Furthermore, we illustrate how chaotic advection can be controlled by regulating the local flow inertia and rotational forces imposed by a combined flow between: i- Poiseuille flow, and ii- Taylor-Couette analogous flow that is generated by interchanging the clockwise/anticlockwise rotation of the inner and outer walls. Results are presented and discussed using Lagrangian tracking and Poincaré sections techniques that describe the different underlying physical phenomena of mixing and heat transfer.

HUBUNGAN PENGETAHUAN DAN PENGAWASAN DENGAN PERILAKU TIDAK AMAN PADA PEKERJA BEKISTING PT BETON KONSTRUKSI WIJAKSANA TAHUN 2020

Unsafe behavior is a human error in taking attitudes and actions. Factors that influence unsafe behavior include predisposing factors, namely knowledge, attitudes, and motivation. Enabling Factors are Availability of PPE and Safety Regulations. Reinforcing Factors are Supervision and Colleagues. Based on the results of observations in September in the field conducted on Formwork Workers of PT Beton Konstruksi Wijaksana, it was found that some unsafe actions were carried out by employees, such as not using PPE properly such as not using a body harness when working at heights and not using PPE. hand protection when using cutting machines, lifting weights in the wrong method chatting and joking while working at work. This study aims to determine the relationship between knowledge and supervision with unsafe behavior in Formwork Workers of PT Beton Konstruksi Wijaksana in 2020. The research method used is quantitative with cross sectional research techniques. To obtain data for 46 respondents, it was done by filling out a questionnaire through google forms with a total sampling technique. The results of the Chi Square test showed that there was a relationship between knowledge and supervision with unsafe behavior among workers in the Formwork Operations Department of PT Beton Konstruksi Wijaksana (P value 0.000). It is expected that the Company can immediately evaluate the K3 program that has been made and plan a new K3 program and provide special training on the function and how to use PPE to all employees considering the importance of using PPE in this formwork work . Keyword : Unsafe behavior, knowledge, the role of supervisors, X V + 7 0 pages : 2 pictture; 8 table, 4 attachment References : 40 (200 0 -2020)

Natal cervical injury and its consequences as a result of caesarean section

The article reflects topical issues of natal trauma and its consequences in children born surgically, with a comparison of different views of domestic and foreign scientists. Risk factors for natal trauma, features of the caesarean section technique, options for complications and outcomes for the newborn are presented. Data from a study of the health of 93 children aged 1 to 16 years who were born surgically by caesarean section were analyzed, approaches to the occurrence of natal trauma and its consequences, as well as their diagnosis and treatment in pediatric patients were considered. It is concluded that the treatment of natal trauma and its consequences after operative delivery should be aimed at eliminating the causes and undesirable symptoms of birth trauma.

Visual inspection of root patterns and radiographic estimation of its canal configurations by confirmation using sectioning method. An ex vivo study on maxillary first premolar teeth.

BackgroundA thorough understanding of the original root and canal anatomy is a critical technical prerequisite for performing cleaning and shaping treatments. Therefore, this research aimed to characterize maxillary first premolar teeth' root morphology and canal architecture.MethodsOne hundred forty-two extracted human adult maxillary first premolar teeth have been retrieved. The extracted teeth were thoroughly cleaned and irrigated to eliminate any remaining debris or blood. They were then preserved in formalin solution until they were eligible for screening. To begin, a visual examination was employed to ascertain the number of roots and their geometry in each sample. Then, utilizing digital radiography in two plains, mesiodistal and distomesial, to further determine those parameters. Finally, a sectioning technique had been used to have the samples cut mesiodistally into slices to validate the internal root canal architecture and identify the number of root canals and their varieties in accordance with Vertucci's categorization system. The canal layout, the pulp chamber, and the root canals were all highlighted (marked) using a fine tip marker in a permanent orange hue to make the canal features more accessible and accurate to visualize. All of the processes were conducted by two highly qualified dentists. The sample size was estimated statistically using the Sealed Envelope program, and the percentage of each configuration was derived in proportion to the overall sample size in order to establish the percentage of each type in each configuration.ResultsFrom the 142 teeth examined, 42 (29.57%) had one root, 97 (68.31%) had two roots, and only three premolars (2.12%) had three roots. Concerning canal configurations, 100 teeth (70.43%) had type (IV) canal configuration, followed by 37 teeth (26.05%) had types (V), three teeth (2.12%) had type (VIII), and one tooth (0.70%) for each of type (I) and type (II).ConclusionsThe anatomical pattern of inspected maxillary first premolars are mainly two rooted and predominantly have a type (IV) canal morphology.

Full functional-length urethral sphincter- and neurovascular bundle preservation improves long-term continence rates after robotic-assisted radical prostatectomy

The objective of the study was to test the impact of implementing standard full functional-length urethral sphincter (FFLU) and neurovascular bundle preservation (NVBP) with intraoperative frozen section technique (IFT) on long-term urinary continence in patients undergoing robotic-assisted radical prostatectomy (RARP). We relied on an institutional tertiary-care database to identify patients who underwent RARP between 01/2014 and 09/2019. Until 10/2017, FFLU was not performed and decision for NVBP was taken without IFT. From 11/2017, FFLU and IFT-guided NVBP was routinely performed in all patients undergoing RARP. Long-term continence (≥ 12 months) was defined as the usage of no or one safety- pad. Uni- and multivariable logistic regression models tested the correlation between surgical approach (standard vs FFLU + NVBP) and long-term continence. Covariates consisted of age, body mass index, prostate volume and extraprostatic extension of tumor. The study cohort consisted of 142 patients, with equally sized groups for standard vs FFLU + NVBP RARP (68 vs 74 patients). Routine FFLU + NVBP implementation resulted in a long-term continence rate of 91%, compared to 63% in standard RARP (p < 0.001). Following FFLU + NVBP RARP, 5% needed 1–2, 4% 3–5 pads/24 h and no patient (0%) suffered severe long-term incontinence (> 5 pads/24 h). No significant differences in patient or tumor characteristics were recorded between both groups. In multivariable logistic regression models, FFLU + NVBP was a robust predictor for continence (Odds ratio [OR]: 7.62; 95% CI 2.51–27.36; p < 0.001). Implementation of FFLU and NVBP in patients undergoing RARP results in improved long-term continence rates of 91%.

Accurate sectional and differential acidizing technique to highly deviated and horizontal wells for low permeable Sinian Dengying formation in Sichuan Basin of China

The Sinian Dengying formation reservoirs in Sichuan Basin of China have characteristics of high temperature, high pressure, deep buried depth, strong heterogeneity, low porosity and permeability, rich pore, natural fractures, vugs and high concentration H2S, CO2, which limit the reconstruction technologies about the gas reservoir. The formation poroperm of Tainei block Dengying unit is worse than that of Taiyuan block’s, and the difference of gas well productions in Tainei block is enormous. The technique of retarded acidizing with segmented by mechanical system was developed and applied in Dengying unit of Taiyuan block, which significantly raised the gas well production. It is difficult for the retarded acidizing technique to applied to gas wells in Tainei block, for the lower formation quality and immature fractures/vugs. The keys to formation reconstruction for highly deviated and horizontal wells are acid homogeneous placement and deep penetrating in order to connecting natural fracture and vugs system. With exploration and practice over several years, a set of segment acid fracturing comprehensive technologies for highly deviated and horizontal wells were formed, including accurate fragmenting based on quantizing evaluation of formation quality, differential acidizing of retarded acid and self-generation acid. The slow acid fracturing technology is used for the formation which the RES is over 1.0. Then, the multistage alternating injection acid fracturing technology is used for the formation which the RES is lower 1.0. The acid fracturing process parameters, such as displacement and acid strength, were optimized by experiments. The precision acidizing technique was applied for gas well of Tainei block Dengying unit, which the testing production of gas well is over 1 million cubic meter per day after aciding stimulated. The precision acidizing technique significantly raised gas well production of Tainei block Dengying unit and is helpful for exploratory development of Tainei block Dengying unit with low formation quality.

Motion about equilibrium points in the Jupiter-Europa system with oblateness

Abstract The study of motion of a test mass in the vicinity of an equilibrium point under the frame of restricted three body problem (RTBP) plays an important role in the trajectory design for different space missions. In this paper, motion of an infinitesimal mass has been described under the frame of Jupiter-Europa system with oblateness. At first, we have determined equilibrium points and then performed linear stability tests under the influence of oblateness of both the primaries. We found that due to oblateness, a considerable deviation in the existing results has occurred. Next, we have computed tadpole and horseshoe orbits in the neighbourhood of triangular equilibrium points and then the oblateness effect is recorded on these orbits. Finally, the evolution of orbits of infinitesimal mass about triangular equilibrium points have been estimated by using Poincaré surface of section technique and it is noticed that in presence of oblateness, quasi-periodic orbit dominates over the chaotic zones. These results will help in further study of more generalised models with perturbations.

Dermatofibrosarcoma Protuberans in Children.

Paediatric dermatofibrosarcoma protuberans (DFSP) is a rare soft tissue malignant tumour which displays aggressive local behaviour and has low metastatic potential. The diagnosis is often delayed as DFSP is usually mistaken for other skin conditions, particularly in the early stages of disease. DFSP tends to follow an indolent course after the initial presentation with what is often described as a "rubbery lump". As the disease progresses, the lump tends to enlarge, change colour, and exhibit a more nodular consistency. In rare cases, DFSP can present as an ulcerated exophytic lesion or a depressed area of skin, making diagnosis even more challenging. A high index of suspicion is warranted for early diagnosis, and referral to a specialist unit with expertise in both oncologic resection and reconstruction. DFSP tumours arise from the dermis and grow with finger-like projections. Therefore, in cosmetically sensitive or functionally important locations, an excision and analysis technique that assesses all excision margins is the gold standard of care. Slow Mohs technique performed with en bloc excision is a well-tolerated option for oncologic resection of the tumour. Mohs technique can also be considered but can be challenging in children for reasons explained below. As an alternative, depending on the anatomical location, tumours can be excised with a wide local excision. While an excision technique that incorporates the deep fascia with a 3-cm peripheral margin is acceptable in adults, planning of the excision margin in children should involve consideration of preoperative imaging with MRI, site of the tumour, age, and physical built of the child. Patients should be offered all treatment options considering the local outcomes, available expertise, and cost. A multidisciplinary approach and good communication between team members is crucial. Close collaboration with a pathologist who is familiar with sectioning technique that allows margin control is of paramount importance. Soft tissue reconstruction should be performed immediately after oncologic clearance, although a staged approach may be required. Adjuvant radiotherapy should be avoided in children due to the long-term risk of secondary malignancies and potential for growth disruption.

Relationship Between Blood Flow and Collapse of Nontraumatic Osteonecrosis of the Femoral Head.

To investigate the collapse mechanism in osteonecrosis of the femoral head (ONFH), we studied the relationship between the femoral head (FH) blood circulation changes and the collapse area histomorphometry characteristics. A technique involving microvascular perfusion of the FH in vitro to reconstruct the vessels in the FH at different stages of nontraumatic ONFH (40 cases). In addition, we also examined the histomorphometry characteristics in the collapse area during ONFH at different stages using the hard tissue section technique. To investigate the blood supply changes in the FH on pathological involved in the FH collapse process. The results showed that in all FHs, the collapse area always involved the margin of the necrotic lesion of the lateral column. Histologically, the fracture occurred between the thickened and necrotic trabeculae at the junction. We found that the collapse started at the lateral column of the FH in the necrotic lesion and that the lateral column was ischemic, which caused the FH to begin to collapse. Based on the above findings, the relationship between associations of the blood circulation to the collapse showed that if a portion of the blood supply of the lateral column (the superior retinacular artery) was preserved, the prognosis of the natural progression of the diseases was improved, the collapse rate was low and collapse occurred later. The blood circulation of artery in the lateral column was good, and the FH maintained an intact shape even if the internal region was ischemic. Therefore, we can predict the collapse of the FH by measuring the blood flow in the lateral area of the FH, thus providing guidance for the selection of FH-preserving clinical therapy in young and middle-aged patients. This work provides a proof of how to predict the collapse of the FH by measuring the blood flow, providing guidance for FH-preserving clinical therapy in young and middle-aged patients.

Hubungan Konsep Diri Dengan Prestasi Akademik Mahasiswa Semeseter 8 Program Studi S1 Keperwatan STIKes Karsa Husada Garut

Human beings as people of learning become an integral part between education and individuals when studying. Education is the mechanism of all-time learning in which a person resides. In order to create conducive learning environment conditions, so that students are able to improve their competence so that they have self-control, spirituality values, ethics and commendable behavior and skills needed by students themselves and the wider community. The direction of this study is to know the relationship of self-concept with the academic achievement of undergraduate nursing students in the 8th semester of Karsa Husada Garut institute. Study method uses descriptive analytics using cross sectional techniques. This study took a population of 70 people and 70 samples were obtain using total sampling techniques. Information obtained using the Q&amp;A instrument that has been provided, through the documentation in Kes Karsa Husada Garut. The validity test used using the correlation "Pearson Moment Product". This study can be concluded, namely: The proportion of students of level IV S1 nursing shows that the majority have a positive self-concept category mostly have a category of good academic achievement. So that the results of the study prove there is a relationship between self-concept and academic achievement of undergraduate nursing students semester eight of Karsa Husada Garut Instute in 2020.

Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas

The Pacific oyster, Crassostrea gigas, is widely distributed and is substantially important to shellfish cultivation. Oysters with melanin-colored shells and soft parts are increasingly popular and are of high economic value. However, the distribution and synthesis of melanin in C. gigas remains unclear and there have been only few relevant studies on it. In this study, transmission electron microscopy (TEM) was used to observe melanin in the epidermis of dark mantle tissue. Melanocytes containing melanosomes were observed below the epidermis, suggesting the possibility of a gradual transfer of melanin from the mantle tissue to the epithelium. The frozen section technique and three melanin staining methods were used to confirm the presence of melanin. The results suggest that enzyme identification (DOPA) is a suitable method of melanin staining in the mantle tissues of C. gigas. This study preliminarily identified the existence of melanin in the mantle tissues of C. gigas and is a foundation for the study of melanin synthesis. Furthermore, it provides new insights into the mechanism of shell color formation.

Agarose-paraffin double embedding assisted with 3D printing for high throughput rice root sectioning

Anatomical analysis of the plant tissues is an important technique used in plant research for studying the process of plant growth, development, adaptation to environmental stress as well as for characterizing gene localization. In Vietnam, most anatomical analysis for plant material was manually conducted which is time-consuming and labor-intensive. In this report, we present an innovative method that can deliver high-quality rice root sectioning. To optimize the efficiency of the conventional paraffin sectioning technique, the agarose-paraffin double embedding was assisted with 3D-printing technique for designing the specific mold which allow to fix from 3 to 12 rice roots in agarose. Molds consist of a lower section with grooves for firmly holding the rice roots in the right place, the additional middle sections allowing up to 3 layers of root samples to be inserted and an upper section to hold the samples before pouring the embedding. The agarose blocks obtained from the molds were then fixed in the formalin solution, then dehydrated through a gradient concentration of ethanol before being impregnated through a series of mixtures of paraffin/xylene and finally embedded in the paraffin. Anatomical sections of 10 μm of thickness were cut with a microtome, spread on glass slides, and then used for staining and collecting the images. As a result, the agarose-paraffin double embedded tissue blocks were more easily orientated and better solidified for rapid and high-quality sectioning. The sections also showed no interference of agarose with staining, as result, the tissues were much clear. In conclusion, this method represents an innovative technique for high throughput plant sectioning which could be applied to different types of plant tissues.

Concordance between Preoperative mpMRI and Pathological Stage and Its Influence on Nerve-Sparing Surgery in Patients with High-Risk Prostate Cancer.

Background: We aimed to determine the concordance between the radiologic stage (rT), using multiparametric magnetic resonance imaging (mpMRI), and pathologic stage (pT) in patients with high-risk prostate cancer and its influence on nerve-sparing surgery compared to the use of the intraoperative frozen section technique (IFST). Methods: The concordance between rT and pT and the rates of nerve-sparing surgery and positive surgical margin were assessed for patients with high-risk prostate cancer who underwent radical prostatectomy. Results: The concordance between the rT and pT stages was shown in 66.4% (n = 77) of patients with clinical high-risk prostate cancer. The detection of patients with extraprostatic disease (≥pT3) by preoperative mpMRI showed a sensitivity, negative predictive value and accuracy of 65.1%, 51.7% and 67.5%. In addition to the suspicion of extraprostatic disease in mpMRI (≥rT3), 84.5% (n = 56) of patients with ≥rT3 underwent primary nerve-sparing surgery with IFST, resulting in 94.7% (n = 54) of men with at least unilateral nerve-sparing surgery after secondary resection with a positive surgical margin rate related to an IFST of 1.8% (n = 1). Conclusion: Patients with rT3 should not be immediately excluded from nerve-sparing surgery, as by using IFST some of these patients can safely undergo nerve-sparing surgery.