Event Abstract Back to Event EM66-containing neurons in the hypothalamic paraventricular nucleus of the rat: No plasticity related to acute immune and thermal stress Fatima-Zohra E. Yamani1, Laurent Yon2, Marlène Guérin2, Abdelilah Alaoui1, Seloua E. Ouezzani1, Nicolas Chartrel2, Youssef Anouar2 and Rabia Magoul1* 1 Université Sidi Mohamed Ben Abdallah, Laboratoire de Neuroendocrinologie et Environnement Nutritionnel et Climatique, Faculté des Sciences Dhar El Mahraz, Morocco 2 Université de Rouen, INSERM U982, Laboratoire Différenciation et Communication Neuronale et Neuroendocrine (DC2N), IFRMP 23, France Neuropeptides, main neuroendocrine system effectors, notably regulate the response to different stressors via a secretory plasticity within their respective hypothalamic neuronal populations. Indeed, the hypothalamic response to an environmental stress implicates the corticotropin-releasing hormone (CRH) neuroendocrine system of the hypothalamic parvocellular paraventricular nucleus (pPVN) in addition to other neuropeptides coexpressed within CRH neurones and controlling the hypothalamo-pituitary-adrenal axis activity as well. The aim of the present study was to explore by immunocytochemistry the occurrence of the neuropeptide EM66 within the parvocellular neurones of the PVN of rat and its potential expression plasticity following stress. The present results have shown that the secretogranin II (SgII)-derived peptide EM66 is strongly expressed within hypothalamic neuroendocrine areas such as the pPVN as well as the median eminence, suggesting a probable hypohysiotropic effect of this peptide. As a first approach to investigate such a role, we evaluated by immunocytochemistry EM66 expression within the pPVN following acute immune stress induced by lipopolysaccharide (LPS, ip) or interleukin – 1ï¢ (ïl ïï ï±ï¢ï ï¬ï icv) ï injection in rat. The results showed that the number of EM66-immunolabeled cells did not fluctuate in this structure following LPS peripheral injection. In line with this observation, an intracerebroventricular injection of ïl ïï ï±ï¢ did not provoke any significant variation of the number of EM66 neurones in the pPVN. Furthermore, EM66 expression level did not change upon an acute thermal stress. In conclusion, the present study revealed for the first time that EM66 expression within the neurosecretory hypothalamic pPVN is not responsive to central and peripheral ïl ïï ï±ï¢. Consequently, EM66 does not participate in the phenotypic plasticity of hypothalamic parvocellular neurons in response to acute inflammatory or thermal stress. Acknowledgment: This study was supported by an exchange program from the INSERM (France) and the CNRST (Morocco) Conference: 2nd NEUROMED Workshop, Fez, Morocco, 10 Jun - 12 Jun, 2010. Presentation Type: Oral Presentation Topic: Oral Session 2: Adaptation to environmental stress Citation: Yamani FE, Yon L, Guérin M, Alaoui A, Ouezzani SE, Chartrel N, Anouar Y and Magoul R (2010). EM66-containing neurons in the hypothalamic paraventricular nucleus of the rat: No plasticity related to acute immune and thermal stress. Front. Neurosci. Conference Abstract: 2nd NEUROMED Workshop. doi: 10.3389/conf.fnins.2010.12.00028 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Jun 2010; Published Online: 04 Jun 2010. * Correspondence: Rabia Magoul, Université Sidi Mohamed Ben Abdallah, Laboratoire de Neuroendocrinologie et Environnement Nutritionnel et Climatique, Faculté des Sciences Dhar El Mahraz, Fès, Morocco, rabea_magoul@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fatima-Zohra E Yamani Laurent Yon Marlène Guérin Abdelilah Alaoui Seloua E Ouezzani Nicolas Chartrel Youssef Anouar Rabia Magoul Google Fatima-Zohra E Yamani Laurent Yon Marlène Guérin Abdelilah Alaoui Seloua E Ouezzani Nicolas Chartrel Youssef Anouar Rabia Magoul Google Scholar Fatima-Zohra E Yamani Laurent Yon Marlène Guérin Abdelilah Alaoui Seloua E Ouezzani Nicolas Chartrel Youssef Anouar Rabia Magoul PubMed Fatima-Zohra E Yamani Laurent Yon Marlène Guérin Abdelilah Alaoui Seloua E Ouezzani Nicolas Chartrel Youssef Anouar Rabia Magoul Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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