Seconds Of Irradiation Research Articles

Proton bunch monitors for the clinical translation of prompt gamma-ray timing

Abstract Objective: Prompt gamma-ray timing is an emerging technology in the field of particle therapy treatment verification. This system measures the arrival times of gamma rays produced in the patient body and uses the cyclotron radio frequency signal as time reference for the beam micro-bunches. Its translation into clinical practice is currently hindered by observed instabilities in the phase relation between the cyclotron radio frequency and the measured arrival time of prompt gamma rays. To counteract this, two proton bunch monitors are presented, integrated into the prompt gamma-ray timing workflow and evaluated. Approach: The two monitors are (a) a diamond detector placed at the beam energy degrader, and (b) a cyclotron monitor signal measuring the phase difference between dee current and voltage. First, the two proton bunch monitors as well as their mutual correlation were characterized. Then, a prompt gamma-ray timing measurement was performed aiming to quantify the present magnitude of the phase instabilities and to evaluate the ability of the proton bunch monitors to correct for these instabilities. Main results: It was found that the two new monitors showed a very high correlation for intermediate proton energies after the first second of irradiation, and that they were able to reduce fluctuations in the detected phase of prompt gamma rays. Furthermore, the amplitude of the phase instabilities had intrinsically decreased from about 700 ps to below 100 ps due to cyclotron upgrades. Significance: The uncertainty of the prompt gamma-ray timing method for proton treatment verification was reduced. For routine clinical application, challenges remain in accounting for detector load effects, temperature drifts and throughput limitations.

Digital Light Processing (DLP) 3D Printing of Caprolactone Copolymers with Tailored Properties through Crystallinity.

Digital light processing (DLP) 3D printing has shown great advantages such as high resolution in the fabrication of 3D objects toward a range of applications. Despite the rapid development of photocurable materials for DLP printing, tailoring properties to meet the specific demands for various applications remains challenging. Herein, we introduce copolymers of caprolactone and allyl caprolactone offering built-in functionality for thiol-ene photochemistry, thereby omitting the need for postfunctionalization. A crystalline block copolymer and an amorphous statistical copolymer were synthesized with the same comonomer composition and molecular weight. Thio-ene photocuring with a tetrafunctional thiol cross-linker was studied at different thiol to double-bond ratios for the copolymers and their blends. All formulations undergo rapid photocuring within several seconds of irradiation with slightly higher gel fractions observed for the statistical copolymer over the block copolymer under the same conditions, suggesting a somewhat higher cross-link density. Thermal properties of the networks were dependent on the presence of the semicrystalline block copolymer, where higher melting enthalpies were reached at higher block copolymer content. Similarly, crystallinity was found to be the main contributor to the mechanical properties. For a comparable composition, the modulus of a block copolymer network was found to be 31 times higher than that of the statistical copolymer network (27.7 vs 0.89 MPa). Intermediate moduli could be obtained by blending the two copolymers. DLP-printed scaffolds from these copolymers retained their thermal properties, therefore offering an efficient approach to tailoring mechanical properties, through crystallinity. Moreover, the printed scaffold displayed shape memory properties as the first example of poly(carprolactone) (PCL) copolymers in DLP printing. These materials are readily synthesized, offer fast and high-resolution 3D printing, and are degradable and cell compatible. They offer a straightforward approach to tailoring properties of PCL-based biomaterials and devices.

A General Molecular Structural Design for Highly Efficient Photopyroptosis that can be Activated within 10 s Irradiation.

Photopyroptosis is an emerging research branch of photodynamic therapy (PDT), whereas there remains a lack of molecular structural principles to fabricate photosensitizers for triggering a highly efficient pyroptosis. Herein, a general and rational structural design principle to implement this hypothesis, is proposed. The principle relies on the clamping of cationic moieties (e.g., pyridinium, imidazolium) onto one photosensitive core to facilitate a considerable mitochondrial targeting (both of the inner and the outer membranes) of the molecules, thus maximizing the photogenerated reactive oxygen species (ROS) at the specific site to trigger the gasdermin E-mediated pyroptosis. Through this design, the pyroptotic trigger can be achieved in a minimum of 10 s of irradiation with a substantially low light dosage (0.4 Jcm⁻2), compared to relevant work reported (up to 60 Jcm⁻2). Moreover, immunotherapy with high tumor inhibition efficiency is realized by applying the synthetic molecules alone. This structural paradigm is valuable for deepening the understanding of PDT (especially the mitochondrial-targeted PDT) from the perspective of pyroptosis, toward the future development of the state-of-the-art form of PDT.

The interplay of chromophore-spacer length in light-induced gold nanocluster self-assembly.

The light-induced self-assembly of chromophore-tethered precision nanoclusters (NCs) has recently received significant attention due to their facile control over structure, function, and reversibility under ambient conditions. However, the magnitude of assembly depends on the photoswitching efficiency, chemical structure, and proximity of the chromophore to the NC surface. Herein, using azobenzene alkyl monothiol (AMT)-capped gold NCs with two different spacer lengths (denoted as C3-NC and C9-NC), we show that reversible cis ↔ trans isomerization efficiency can be readily tuned to control the self-assembly kinetics of NCs. Irrespective of the chain length, the time required for trans-to-cis (140 s) and cis-to-trans (260 s) isomerization of individual C3-AMT and C9-AMT is identical in dichloromethane solution. When a similar experiment was performed using a solution of C3-NCs and C9-NCs, it resulted in self-assembled disc-like superstructures. Notably, the trans-to-cis photoswitching in C3-NC could reach only 65% even after 460 seconds of irradiation. On the other hand, C9-NC completed this process within 160 seconds of irradiation. The low photoswitching efficiency of the C3-NC analog is due to the short and rigid spacer length of C3-AMT ligands, which are in close proximity to the NC surface, resulting in steric hindrance experienced at the NC-chromophore interface. Importantly, the slow photoswitching in C3-NCs helps isolate and investigate the intermediates of assembly. Using high-resolution electron microscopy, atomic force microscopy, and 3D reconstruction, we show that the discs are made up of densely packed arrays of NCs. The prolonged illumination of C9-NCs results in a chain-like assembly due to the dipolar attraction between the previously assembled superstructures. The efficient photoisomerization of chromophores located away from the nanocluster surface has been identified as the key element to speed up the light-induced assembly in chromophore-tethered nanoclusters. Such information will be useful while developing nanoscale photoswitches for electrochemistry, biosensors, and electronic devices.

Degradation processes of brominated flame retardants dispersed in high impact polystyrene under UV-visible radiation.

In order to protect human health and the environment, several regulations have been introduced in recent years to reduce or even eliminate the use of some brominated flame retardants (BFRs) due to their toxicity, persistence and bioaccumulation. Dispersions of these BFRs in polymers are widely used for various applications. In this report, four different brominated molecules, decabromodiphenyl ether (DBDE), hexabromocyclododecane (HBCDD), decabromodiphenyl ethane (DBDPE) and tris(tribromophenoxy)triazine (TTBPT), were dispersed in the solid matrix of an industrial polymer, high impact polystyrene (HIPS). The possibility of degradation of these BFRs within HIPS under UV-visible irradiation in ambient air was investigated. The degradation kinetics of DBDE and HBCDD were followed by Fourier transform infrared spectroscopy (FTIR) and high-resolution two-step laser mass spectrometry (L2MS). The thermal properties of the pristine and irradiated polymer matrix were monitored by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), which showed that these properties were globally preserved. Volatile photoproducts from the degradation of DBDE, DBDPE and TTBPT were identified by headspace gas chromatography/mass spectrometry analysis. Under the chosen experimental conditions, BFRs underwent rapid degradation after a few seconds of irradiation, with conversions exceeding 50% for HIPS/DBDE and HIPS/HBCDD systems.

Laser-Induced Temperature Changes on the Outer Surface of Titanium Dental Implants: An In Vitro Study.

To measure the surface temperature distribution after using a CO2 laser to heat titanium dental implants via different power settings, application intervals, and irradiation times. A total of 10 tissue-level titanium implants (Screw-Line Promote Plus, Camlog; 4.3 × 11 mm) were placed (EpoFix, Struers) and irradiated with a CO2 laser (Denta 2, Lutronic) with a wavelength of 10.6 μm at power levels of 4 watts (Group 1), 6 watts (Group 2), 8 watts (Group 3), and 10 watts (Group 4). A continuous beam mode (setting I) and noncontinuous beam modes with 5-second (setting II) and 10-second (setting III) pause intervals were used. For each setting, a total irradiation time of 50 seconds was used and repeated 10 times. The temperature was measured using external thermocouple (Testo) in contact with the implant surface at the implant shoulder, middle, and apex. A linear regression model was used to analyze the data (P = .05). Setting I demonstrated the most rapid increase in implant surface temperature in all three test sites as well as the greatest total temperature at 50 seconds of irradiation time. The greater the pause interval (settings II and III) during the 50 seconds of irradiation, the lower the rate of temperature increase as well as the total temperature in all three test sites and with all power levels. The average temperature difference between the apex and shoulder site was significant for setting III for all groups, but not for any groups in settings I and II. Heating the internal aspect of an implant with a CO2 laser produces different temperature distribution profiles depending on the laser power level and the application interval. Laser-beam irradiation leads to a temperature gradient, which is greatest at the implant apex and smallest at the implant shoulder.

Two-Photon Light Trigger siRNA Transfection of Cancer Cells Using Non-Toxic Porous Silicon Nanoparticles.

The concept of using two-photon excitation in the NIR for the spatiotemporal control of biological processes holds great promise. However, its use for the delivery of nucleic acids has been very scarcely described and the reported procedures are not optimal as they often involve potentially toxic materials and irradiation conditions. This work prepares a simple system made of biocompatible porous silicon nanoparticles (pSiNP) for the safe siRNA photocontrolled delivery and gene silencing in cells upon two-photon excitation. PSiNP are linked to an azobenzene moiety, which possesses a lysine group (pSiNP@ICPES-azo@Lys) to efficiently complex siRNA. Non-linear excitation of the two-photon absorber system (pSiNP) followed by intermolecular energy transfer (FRET) to trans azobenzene moiety, result in the photoisomerization of the azobenzene from trans to cis and in the destabilization of the azobenzene-siRNA complex, thus inducing the delivery of the cargo siRNA to the cytoplasm of cells. Efficient silencing in MCF-7 expressing stable firefly luciferase with siRNAluc against luciferase is observed. Furthermore, siRNA against inhibitory apoptotic protein (IAP) leads to over 70% of MCF-7 cancer cell death. The developed technique using two-photon light allows a unique high spatiotemporally controlled and safe siRNA delivery in cells in few seconds of irradiation.

Effectiveness of 980nm diode Laser in reduction the diameters of exposed dentinal tubules for hypersensitive tooth

Abstracts: Background: The oral cavity is a complex environment, both structurally and functionally, the hard and soft tissues are in close a proximity. Oral tissues subjected to wear throughout the life, that threatened the vitality of the pulp or increase the sensitivity of dentinal tubules. One of the common dental problems is loss of enamel or cementum, which stimulate the nerve ending in or near the pulp and manifested as pain sensation. Aim of the study: This study had done to evaluate the effects of 980nm diode Laser in diameters reduction of exposed dentinal tubules analyze the results and morphological changes of irradiated dentine surface by FE-SEM (field emission scanning electron microscope) analysis. Material and Method: Thirty-nine human extracted impacted 3rd molar teeth were tested in this study and divided into control Group (N=15), pilot study (N=9) and study group (N=15). The study group irradiated with 980nm diode Laser to the occlusal surface of the teeth after the application of 17% EDTA (Ethelyn diamine tetra acetic acid) for smear layer removal. The intra-pulpal temperature recorded for every second and monitored for 120s from the first second of irradiation by a thermometer (prośket MT-1820, 1st Edition, 2016, Taiwan), for detection the recovery time of initial tooth temperature. FE-SEM analysis to all teeth (control, pilot and study groups) for assessment the morphological changes after surface irradiation. Results: FE-SEM analysis to the irradiated surface show significant reduction in diameters of the exposed dentinal tubules irradiated by 980nm diode Laser. Conclusion: The selected parameters (1.5W/10s, 276.3W/cm2) were able to seal exposed dentinal tubules without any sign of cracks or fissures. Suggestion: The parameters used with this study (1.5W, 10s, 276.3W/cm2) can be utilize in the future treatment protocol to the management of dentinal hypersensitivity.

The Impact of Different Rose Bengal Formulations on Corneal Thickness and the Efficacy of Rose Bengal/Green Light Corneal Cross-linking in the Rabbit Eye.

To examine central corneal thickness (CCT) changes during in vivo rose bengal-green light corneal cross-linking (RG-CXL) and compare the CXL efficacy of different rose bengal formulations. After epithelium removal, the right eyes of rabbits were immersed in rose bengal solution prepared by different solvents (water, phosphate buffered saline, dextran, and hydroxypropyl methylcellulos [HPMC]) for 2 or 20 minutes, then the rose bengal distribution in the corneal stroma was analyzed by confocal fluorescence detection. During the RG-CXL process, the CCT was measured at seven time points. The left eyes served as the untreated control group. Corneal enzymatic resistance and corneal biomechanics were tested to compare the RG-CXL efficacy. The rose bengal infiltration depths were 120 and 200 µm for the 2- and 20-minute groups, respectively. CCT increased significantly after infiltration, then decreased significantly in the first 200 seconds of irradiation and decreased slowly for the next 400 seconds. The CCT of the 20-minute groups was significantly thicker than that of the 2-minute groups (P < .0001). All RG-CXL treatments improved the corneal enzymatic resistance and corneal biomechanics, with the effects being greater in the 20-minute groups. The inclusion of 1.1% HPMC in the rose bengal formulation helped to maintain CCT during irradiation while not affecting either the infiltration of rose bengal or the efficacy of RG-CXL. Within the range studied, RG-CXL efficacy increased with infiltration time. The incorporation of a 20-minute infiltration of 0.1% rose bengal-1.1% HPMC into the RG-CXL procedure may further improve the safety of the treatment and its prospects for clinical use. [J Refract Surg. 2022;38(7):450-458.].

DOSE MAPPING OF GAMMA IRRADIATION CHAMBER (GIC)

The use of gamma rays to irradiate blood products have been demonstrated to reduce the probability of post-transfusion graft versus host disease. The aim of this study is to investigate the accuracy of a new batch of Gafchromic EBT-XD films to map the homogeneity of gamma radiation dose delivered to the blood bags and identify the lowest and highest levels of radiation exposure to the blood. The Gammacell 3000 Elan Gamma irradiation chamber (GIC) @ Blood Irradiator with Cesium-137 source was used. During the gamma irradiation at an exposure of 8 minutes 51 seconds to deliver a central dose of 25 Gy to the film, the EBT-XD films were placed at the centre of a blood-equivalent phantom. The doses then were measured using dose mapping analysis of Gafchromic EBT-XD film. The responses of EBT-XD films were compared with EBT3 films and GS 300 Dose Mapping Report 2021 prepared by Best Theratronics Dosimetry Laboratory, Canada. Results showed the value of doses obtained from the mapping using EBT-XD were reliable compared with the doses measured in EBT3 film as they were in the range of the acceptable dose ranges for blood irradiation procedures. The mapping with EBT-XD film yielded a minimum dosage of 18 Gy and a maximum exposure of 32 Gy at 8 minutes 51 seconds of irradiation. In conclusion, EBT-XD offered excellent dosimetric characteristics and has a the potential to dose map the blood irradiator.

A new detector for the beam energy measurement in proton therapy: a feasibility study

The proof of concept of a new device, capable of determining in a few seconds the energy of clinical proton beams by measuring the time of flight (ToF) of protons, is presented. The prototype consists of two thin ultra fast silicon detector (UFSD) pads, aligned along the beam direction in a telescope configuration and readout by a digitizer. The method developed for extracting the energy at the isocenter from the measured ToF, validated by Monte Carlo simulations, and the procedure used to calibrate the system are also presented and discussed in detail. The prototype was tested at the Centro Nazionale di Adroterapia Oncologica (CNAO, Pavia, Italy), at several beam energies, covering the entire clinical range, and using different distances between the sensors. The measured beam energies were benchmarked against the nominal CNAO energy values, obtained during the commissioning of the centre from the measured ranges in water. Deviations of few hundreds of keV have been achieved for all considered proton beam energies for distances between the two sensors larger than 60 cm, indicating a sensitivity to the corresponding beam range in water smaller than the clinical tolerance of 1 mm. Moreover, few seconds of irradiation were necessary to collect the required statistics. These preliminary results indicate that a telescope of UFSDs could achieve in a short time the accuracy required for the clinical application and therefore encourage further investigations towards the improvement and the optimization of the present prototype.

Ultrafast Epoxy‐Anhydride Photopolyaddition Reaction

AbstractThe conventional epoxy/anhydride reaction is quite slow (more than one hour to reach full conversion at room temperature (RT)) and usually requires thermal treatment at rather high temperature. Ideally, the reaction should be triggered by light for on demand systems i.e. very fast polymerizations (in few seconds) can be carried out in mild conditions (at RT with low energy consumption using cheap and safe light emitting diode (LED) light). In this work, a new approach is proposed with an ultrafast epoxy/anhydride photopolyaddition reaction that can be triggered by near‐UV/visible light. Therefore, a photoinitiating system is developed to cure epoxides in presence of anhydrides as hardeners without any external heat and in a short time scale i.e. few seconds upon light vs. hours at RT without light. Excellent polymerization performances can be observed upon LED@405nm irradiation with full epoxy function conversion. The polymerization kinetics are followed both by real‐time FTIR and real‐time photorheology experiments. As example of the high performance of this new photoactivated epoxy/anhydride process, the curing of epoxy/glass fibre composites is presented. Remarkably, an excellent depth of cure can be obtained in only 20 seconds of irradiation with a LED@405 nm.

Green and selective oxidation of alcohols using MnO2 nanoparticles under solvent-free condition using microwave irradiation

Alcohols are selectively oxidized to aldehydes using active manganese dioxide (MnO2) nanoparticles under solvent-free condition using microwave irradiation. The complete conversion of the alcohols to their corresponding aldehydes was achieved successfully within only 15 seconds of irradiation in microwave oven. The products of oxidation were characterized by TLC, IR, and Tollens' test, which showed the characteristic results of the desired aldehydes. This method is found to be fast, green, and a very easy procedure that could be applied for the oxidation of alcohols to aldehydes without further oxidation to carboxylic acids.

Fast Method for Testing the Photocatalytic Performance of Modified Gypsum

The measurement of the photocatalytic activity of building materials is quite time-consuming. Up until now, researchers have mainly used the equipment described in ISO 22197-1 to ISO 22197-4 for the determination of air purification activity, although other apparatus such as colorimeters, UV-Vis/DR spectroscopes and equipment for contact angle measurements have also been used. Usually, photocatalytic activity measurements take from one hour up to several hours. In this study, we present a very fast method for the measurement of the photocatalytic activity of gypsum. A specially designed printer with a modified bubblejet cartridge was used to apply a special ink on the surface of gypsum plates. Then the surface was irradiated by UV-A light and every 3 s a picture of the surface was taken. The results showed that the discoloration of the dye occurs after a few seconds of irradiation and the time depends on the amount of photocatalyst used as well as the number of printed ink layers. It was concluded that it is possible to use this method for a quick comparison of the photocatalytic activity of different types of modified gypsum materials.

Effect of duration of Er,Cr:YSGG laser etching on dentin morphology: an in vitro study

The aim of this study was to evaluate the effect of different durations of Er,Cr:YSGG laser irradiation on dentin morphology using scanning electron microscopy (SEM). Twenty-five extracted human-impacted permanent third molars were cut below the occlusal pit and fissure level, perpendicular to the long axis of the tooth. An occlusal area measuring 5 mm in length and 5 mm in width was prepared on each tooth for laser irradiation. The teeth were randomly divided into five groups for different durations of laser irradiation: T1, 5 s; T2, 10 s; T3, 20 s; T4, 40 s; T0, no laser irradiation. The effects of laser application on dentin surfaces were evaluated using SEM at × 80 and × 500 magnifications. Also, the presence/absence of smear layer was scored according to SEM findings. Score 0 indicated the absence and score 1 indicated the presence of smear layer. Forty seconds of irradiation resulted in an irregular dentin surface without smear layer, with open dentinal tubules and without enlargement. Laser preparation of dentin creates a retentive surface for composite restoration, without the problems caused by smear layer after conventional preparation. Also, the disadvantages of acid etching can be avoided as such.

Expression of heat shock protein 70 and cell death kinetics after different thermal impacts on cultured retinal pigment epithelial cells

Recent technologies are broadening the possibility to treat the retinal pigment epithelium (RPE) with different thermal impacts, from sublethal to lethal ranges. Thus temperature-dependent subcellular molecular responses need to be elucidated in more detail. In this study, RPE cell viability and expression of heat shock protein 70 (Hsp70) were investigated after thermal irradiation with different temperature increase using an in-vitro model. Primary porcine RPE cell cultures were irradiated with different laser power of a thulium laser (λ = 1940 nm, beam-diameter 30 mm) for 10 s, such that the maximal temperatures at the center of the culture dish (Tmax) reach 40, 44, 47, 51 or 59 °C after 10-s irradiation. The temperature distribution across the culture dish shows a Gaussian decay from central position to the periphery of the dish. At 3, 24 and 48 h after irradiation cell viability was assessed with fluorescence microscopy using cell viability-indicating fluorescence dyes, followed by the determination of the threshold temperature for apoptotic change and death of RPE cells. Intracellular localization and amount of Hsp70 were investigated with immunofluorescence and western blotting, respectively. The threshold temperature (at the 10th second of irradiation: T10s) for cellular apoptosis and complete cell death showed a decrease over time after irradiation, suggesting a long-term process of thermally induced cell death. For complete cell death the threshold T10s was 52.1 ± 0.6 °C, 50.1 ± 1.4 °C, and 50.1 ± 0.8 °C, for 3, 24 and 48 h, respectively, whereas for the apoptotic changes 48.6 ± 1.8 °C, 47.2 ± 1.3 °C, and 46.7 ± 0.9 °C, respectively. Quantitative analysis of Hsp70 with western blotting showed a significant increase in intracellular Hsp70 at lethal irradiation with Tmax ≥ 51 °C, up to 19.6 ± 2.3 fold after 48 h at 59 °C, whereas sub-lethal irradiations with Tmax ≤ 44 °C led to a slight tendency of time-dependent increases (up to 1.8 ± 1.1 fold) over 48 h. Immunostainings for Hsp70 showed a circle- or ring-pattern of the Hsp70 staining during 3-48 h after irradiation, and the range of the 1st and 3rd quartiles of T10s for heat-induced Hsp70 expression over this time period was between 44.8 °C and 48.2 °C. A very strong staining of Hsp70 was observed at the border to the damaged zone, where many cells show the strong staining in the whole cytoplasmic space, while some cells in the nucleus, or some cells show the signs of cell migration and proliferation. Moreover, among the cells showing high intensity of Hsp70 staining, there are small round cells like apoptotic cells. Results suggest that RPE cell death after thermal irradiation may take time, and mostly undergoes through apoptosis, unless cells are immediately killed. Thermal irradiation-induced Hsp70 expression is not only temperature-dependent, but also depends largely on the existence of neighboring cell death, suggesting the crucial role of Hsp70 in apoptosis and wound healing processes of RPE cells. The increase of Hsp70 over 24-48 h indicates its long-term roles in cell responses both after sublethal and lethal thermal laser irradiations.

Optogenetic Manipulation of Cyclic Di-GMP (c-di-GMP) Levels Reveals the Role of c-di-GMP in Regulating Aerotaxis Receptor Activity in Azospirillum brasilense

Bacterial chemotaxis receptors provide the sensory inputs that inform the direction of navigation in changing environments. Recently, we described the bacterial second messenger cyclic di-GMP (c-di-GMP) as a novel regulator of a subclass of chemotaxis receptors. In Azospirillum brasilense, c-di-GMP binds to a chemotaxis receptor, Tlp1, and modulates its signaling function during aerotaxis. Here, we further characterize the role of c-di-GMP in aerotaxis using a novel dichromatic optogenetic system engineered for manipulating intracellular c-di-GMP levels in real time. This system comprises a red/near-infrared-light-regulated diguanylate cyclase and a blue-light-regulated c-di-GMP phosphodiesterase. It allows the generation of transient changes in intracellular c-di-GMP concentrations within seconds of irradiation with appropriate light, which is compatible with the time scale of chemotaxis signaling. We provide experimental evidence that binding of c-di-GMP to the Tlp1 receptor activates its signaling function during aerotaxis, which supports the role of transient changes in c-di-GMP levels as a means of adjusting the response of A. brasilense to oxygen gradients. We also show that intracellular c-di-GMP levels in A. brasilense change with carbon metabolism. Our data support a model whereby c-di-GMP functions to imprint chemotaxis receptors with a record of recent metabolic experience, to adjust their contribution to the signaling output, thus allowing the cells to continually fine-tune chemotaxis sensory perception to their metabolic state.IMPORTANCE Motile bacteria use chemotaxis to change swimming direction in response to changes in environmental conditions. Chemotaxis receptors sense environmental signals and relay sensory information to the chemotaxis machinery, which ultimately controls the swimming pattern of cells. In bacteria studied to date, differential methylation has been known as a mechanism to control the activity of chemotaxis receptors and modulates their contribution to the overall chemotaxis response. Here, we used an optogenetic system to perturb intracellular concentrations of the bacterial second messenger c-di-GMP to show that in some chemotaxis receptors, c-di-GMP functions in a similar feedback loop to connect the metabolic status of the cells to the sensory activity of chemotaxis receptors.

Effect of 5-Substitution of Uracil Base in DNA Photocrosslinking Using 3-Cyanovinylcarbazole

Oligonucleotide containing 3-cyanovinylcarbazole (CNVK) can crosslink to a pyrimidine base in complementary DNA/RNA by a few seconds of irradiation. The effect of 5-substitution of the uracil base in DNA photocrosslinking using CNVK was evaluated. Each uracil analog base had diverse photoreactivity with CNVK, and there was good correlation with the photoreactivity and LUMO level of the target uracil base in duplex DNA.

Portable UV light as an alternative for decontamination

We evaluated the capability of a commercially available hand-held device that emits ultraviolet (UV) light to disinfect plain surfaces. Eight bacterial species were tested, including Clostridium difficile ribotype 027 and 3 other spore-forming species. Even bacterial spores could be successfully inactivated within a few seconds of irradiation. UV light may provide an alternative for the decontamination of medical products, such as mobile phones or tablet computers, that cannot be treated otherwise.

Range assessment in particle therapy based on prompt γ-ray timing measurements

Proton and ion beams open up new vistas for the curative treatment of tumors, but adequate technologies for monitoring the compliance of dose delivery with treatment plans in real time are still missing. Range assessment, meaning the monitoring of therapy-particle ranges in tissue during dose delivery (treatment), is a continuous challenge considered a key for tapping the full potential of particle therapies. In this context the paper introduces an unconventional concept of range assessment by prompt-gamma timing (PGT), which is based on an elementary physical effect not considered so far: therapy particles penetrating tissue move very fast, but still need a finite transit time—about 1–2 ns in case of protons with a 5–20 cm range—from entering the patient’s body until stopping in the target volume. The transit time increases with the particle range. This causes measurable effects in PGT spectra, usable for range verification. The concept was verified by proton irradiation experiments at the AGOR cyclotron, KVI-CART, University of Groningen. Based on the presented kinematical relations, we describe model calculations that very precisely reproduce the experimental results. As the clinical treatment conditions entail measurement constraints (e.g. limited treatment time), we propose a setup, based on clinical irradiation conditions, capable of determining proton range deviations within a few seconds of irradiation, thus allowing for a fast safety survey. Range variations of 2 mm are expected to be clearly detectable.