Secondary Metabolite Compounds Research Articles

Antibacterial Activity of Extract Green Seaweed Ulva intestinalis in North Waters of Aceh

The appearance of bacterial resistance and pathogenic infections makes efforts to find new drugs as natural bacterial agents continue. One of them is through the utilization of marine organisms such as seaweed. Some studies show the content of seaweed bioactive compounds has potential in the pharmacological field. This study aims to determine the type of secondary metabolite compounds and antibacterial activity of ethanol extract of Ulva intestinalis. Seaweed extraction using the maceration method for 3 x 24 hours and antibacterial activities were tested by disc diffusion method. The results showed that there were 5 types of secondary metabolite compounds in the extract, namely alkaloids, flavonoids, glycosides, saponins, and steroids. The antibacterial activity test of seaweed extract against Propionibacterium acnes and Aeromonas hydrophila bacteria in the concentration range of 50% to 100% showed a moderate inhibition zone diameter

Effects of Low-Dose Kinetin, 2,4-D and Monochromatic Light Conditions on Flavonoid Content in Callus Culture of Dioscorea esculenta

The callus culture of Dioscorea esculenta is a potential strategy to produce efficacious bioactive compounds in multiplied production. It can be carried out by applying plant growth regulators (PGR) and environmental modification, including light-emitting diodes (LEDs) exposure. This study aims to investigate the impact of PGR and the stimulatory effect of LEDs on flavonoid profiles in D. esculenta callus culture. The study was conducted using combination variations of 2,4-D (0.5 and 1 ppm), kinetin (0.5 and 1 ppm), and LEDs stimulatory conditions (light and dark). The results showed that flavonoids are the dominant compounds found in lesser yam cultures, which reached more than 78 % in all treatments-specifically, adding 0.5 ppm kinetin and 0.5 ppm 2,4-D to D. esculenta callus cultures significantly increased flavonoids production compared to single PGR treatment. This shows there is a synergistic activity induced by 2,4-D and kinetin. Furthermore, monochromatic light exposure also significantly affects flavonoid production. Analysis of 5 main flavonoids, isoflavones, flavanones, kaempferols, quercetin, and epigallocatechin, proved that monochromatic light significantly increased flavonoids production compared to callus cultures were kept in a growth room under 24 °C temperature in the dark condition. This study confirmed that equally combining 0.5 ppm of 2,4-D and 0.5 ppm of kinetin under monochromatic LEDs increases flavonoid production in D. esculenta callus cultures. Further research must be conducted to describe how the bioactive compound synthesis signaling pathway involving PGR and monochromatic LEDs improves plant quality in the pharmaceuticals’ role. HIGHLIGHTS Kinetin and 2,4-D at the same concentration of 0.5 ppm is an optimal combination to increase secondary metabolite production in esculenta callus culture Combining kinetin and 2,4-D at low concentrations significantly increased flavonoids, especially kaempferol, isoflavone, quercetin, flavanone, and epigallocatechin in esculenta callus culture Light condition significantly affects the production of flavonoids, especially kaempferol, isoflavone, quercetin, flavanone, and epigallocatechin There is evidence that kinetin and 2,4-D under light conditions work in a synergic effect on the biosynthesis of secondary metabolite compounds in esculenta callus cultures GRAPHICAL ABSTRACT

Total Metabolit Sekunder dan Uji Aktivitas Antioksidan Daun Juwet (Syzygium cumini L.) dengan Spektrofotometer Uv-Vis

The aim of this research is to determine the total amount of secondary metabolites (alkaloids, flavonoids, saponins and tannins) contained in the ethanol juice of juwet leaves (Syzygium cumini L.) using the UV-Vis spectrophotometry method and to determine the antioxidant activity of the ethanol juice of juwet leaves based on IC50 value. Determination of total levels of secondary metabolites using UV-Vis spectrophotometry and antioxidant testing using the DPPH method. The results of qualitative analysis of the ethanol juice of juwet leaves contained secondary metabolite compounds, namely flavonoids, saponins, tannins and alkaloids. The average value obtained for the level of total secondary metabolites for flavonoid compounds was 0.417% w/w., saponins 0.766 w/w., tannins 0.282 w/w and alkaloids 0.384% w/w. The results of testing the antioxidant activity of juwet leaf ethanol extract and quercetin as a comparison had antioxidant activity, the average IC50 results were 14.289 ± 0.255 ppm and 102.45 ppm, therefore they were classified as moderate antioxidants.

ANTIOXIDANT PROPERTIES OF THE ETHYL ACETATE EXTRACT OF ENDOPHYTIC FUNGUS Penicillium Citrinum FROM Kalanchoe Millotii STEM THROUGH SECONDARY METABOLITES

Kalanchoe, as Cocor Bebek, is a genus of plants that is often used in traditional medicine because it has various biological activities, one of which is as an antioxidant. The development of research was carried out on the secondary metabolites compounds of endophytic fungi from the stem of K. millotii as the Cocor Bebek species. Endophytic fungi are microorganisms that live in plant tissues, generally producing secondary metabolites that have bioactivities such as anticancer, antioxidant, and antibacterial. One of the endophytic fungi isolated from the stem of K. millotii was identified as Penicillium citrinum. The potential antioxidant activity of the secondary metabolites contained in the ethyl acetate extract of the endophytic fungus P. citrinum is interesting to study. The sample extract was extracted using non-polar, semi-polar, and polar solvents. The ethyl acetate extract was separated and purified by various chromatographic methods to obtain 8 mg compound 1, as a yellow solid. The chemical structure was determined by spectroscopic methods. Based on spectrum analysis of 1D-NMR, 2D-NMR, FTIR, and UV-Vis, compound 1 was identified as 4-(2-ethylbutoxy)-3,5-dimethoxybenzaldehyde. Antioxidant activity testing of ethyl acetate extract and compound 1 was carried out using the DPPH (2,2-diphenyl-1-pikrilhidrazyl) method. The test results on the extract showed weak activity with an IC50 value of 401.01 ppm while compound 1 showed moderate activity with an IC50 value of 221.41 ppm

Pengaruh Ekstrak Metanol Buah Lakum (Cayratia trifolia L. Domin) terhadap Nilai SGOT dan SGPT Tikus Putih Diinduksi Parasetamol

Lakum fruit (Cayratia trifolia L. (Domin)) has secondary metabolite compounds that are beneficial to health, one of which is a hepatoprotector. This study was conducted to prove the potential of hepatoprotective activity of lakum fruit methanol extract based on the value of SGPT and SGOT enzyme. The research method used a pre-posttest randomized control group design with a sample of 35 male rats divided into five treatment groups: solvent control group (CMC 0.5%); positive control group (HEPA-Q®); treatment group extract (0,575; 1,15; 1,725 mg/g BW). The treatment of induction of paracetamol and methanol extract of Cayratia trifolia fruit was carried out for 7 days respectively. The results showed that the dose of 1,15 mg/g BW of Cayratia trifolia fruit methanol extract had the most effective deterioration of SGOT and SGPT values with a percentage reduction of SGOT of 49.3% and SGPT of 51.8% (p <0.05) which approached control positive (HEPA-Q®). Based on the research conducted, methanol extract of Cayratia trifolia fruit has the potential to be hepatoprotective.

Bioactivities of Nek Kara (Dillenia excelsa (Jack) Martelli ex. Gilg) leaves from forest medicinal plants of Kutai Ethnic

The utilization of traditional medicine and treatment within the community of Kutai Kartanegara, East Kalimantan, Indonesia is characterized by a wide range of practices. Medicinal plant extracts containing secondary metabolites, possessing diverse molecular structures and biological activities, exhibit promising potential for the development of medicines targeting various diseases. Phenolic compounds, present in plants, exhibit numerous biological effects, including antioxidant and antimicrobial properties. These secondary metabolite compounds play a crucial role in safeguarding against disease-induced damage. Nek Kara (Dillenia excelsa (Jack) Martelli ex. Gilg), a forest plant, has been traditionally employed by the local population for the treatment of diabetes. The objective of this study is to investigate the biological activities of the ethanol extract derived from Nek Kara leaves. The analysis encompasses the examination of phytochemical content, toxicity, as well as antioxidant and antimicrobial activities of the extract. The antioxidant activity is assessed through DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activity using a spectrophotometer, while toxicity is determined via the BSLT (Brine Shrimp Lethality Test). The antimicrobial activity is evaluated using the diffusion method. The findings reveal the presence of phytochemical content, such as alkaloids, flavonoids, tannins, and steroids, within the ethanol extract derived from Nek Kara leaves. The BSLT results indicate the absence of toxic bioactivities in the ethanol extract. Furthermore, the Nek Kara leaf extract demonstrates potent antioxidant activity and exhibits inhibitory effects on the growth of bacterial and fungal colonies.

Gas Chromatography-Mass Spectrometry and Functional Group Analysis of Methanol Extract of Bidara Leaves (Ziziphus mauritiana)

Bidara leaves are a plant that is quite abundant in Medan and has many benefits in the health sector. The aim of this research is to predict the content of secondary metabolite compounds in bidara leaf extract, through qualitative tests by screening using specific reagents, functional group analysis with FT-IR and mass analysis with GC-MS. The screening results showed that the methanol extract of bidara leaves contained alkaloids, flavonoids, tannins, saponins and phenols. Functional group analysis shows that the wavelength area is 3300 cm-1, namely OH absorption, 2700 - 2900 cm-1 shows C-H absorption, 1600 - 1700 cm-1 shows C=O absorption, 1400 - 1450 cm-1 shows C-H absorption, 1365 - 1385 cm-1 shows CH3 bent absorption, and 1000 – 1100 cm-1 shows C-O absorption. The results of mass analysis using GC-MS showed four dominant peaks from the compound, namely Z-5-Methyl-6-heneicosen-11-one, 17-Pentatriacontene, Ethyl iso-allocholate and Pentacosane.

Beta-sitosterol glycoside from Paraboea leuserensis and Cytotoxicity Test against MCF-7 Human Breast Cancer Cells

Background: Paraboea leuserensis, a plant endemic to the Leuser Mountain in the provinces of Aceh and North Sumatra, has been traditionally used for medicinal purposes by chewing or boiling, addressing conditions such as stomachaches and providing stamina enhancement. This study aimed to isolate secondary metabolite compounds from Paraboea leuserensis and conduct in vitro anticancer tests.
 Method: The ethyl acetate extract was isolated through column chromatography, yielding beta-sitosterol glycoside, and was tested for bioactivity against human breast cancer cells MCF-7 using the MTT method.
 Results: The compound demonstrated activity against breast cancer cells (MCF-7) with an IC50 value of 24.83 mg/L.
 Conclusion: Paraboea leuserensis exhibits potential anticancer activity. The isolation of beta-sitosterol glycoside from the plant and its demonstrated activity against MCF-7 human breast cancer cells suggest a promising avenue for further exploration of the plant's anticancer properties.

Phytochemical screening and antioxidant activity test of ethanol extract of casturi mango leaves (Mangifera casturi Koesterm.) from Drien Bungong village, Pidie Jaya, using the DPPH method

Antioxidants are chemical compounds that, in specific amounts, can inhibit or slow down cell damage caused by free radicals. Antioxidants function to protect the body from free radical attacks. The more free radicals in the body, the more cells will be damaged. One plant that contains antioxidant compounds is casturi mango (Mangifera casturi Koesterm.). Casturi mango (Mangifera casturi Koesterm.) is a plant that contains secondary metabolite compounds such as saponins, tannins, triterpenoids, flavonoids, and phenolics which have potential as antioxidants. This research aimed to determine differences in secondary metabolite compounds and antioxidant activity of ethanol extract of casturi mango leaves (Mangifera casturi Koesterm.) growing in Gampong Drien Bungong, Pidie Jaya. The stages of this research include sample processing, making ethanol extract of kasturi mango leaves, characterization examination, phytochemical screening, and antioxidant activity test. The sample used was musk mango leaves. The ethanol extract of casturi mango leaves (Mangifera casturi Koesterm.) was extracted using maceration with 96% ethanol solvent. The antioxidant activity test was carried out using the DPPH (1-1-Diphenyl-2-Picrylhydrazyl) method using a UV-Vis spectrophotometer at a maximum wavelength of 516 nm and vitamin C as a comparison. Based on the results of phytochemical screening tests, there are differences in secondary metabolites in the ethanol extract of kasturi mango leaves in Gampong Drien Bungong, Pidie Jaya, namely alkaloids, flavonoids, tannins, saponins, steroids, and glycosides, and the results of antioxidant activity obtained an IC50 value of 9.06 ppm with the category very strong, equivalent to the antioxidant potential of vitamin C, having an IC50 value of 4.63 ppm in the robust category.

Effectiveness of green betel leaf extract cream in healing cut wounds

Green betel leaf (Piper betle L.) is widely used in the healing of cut wounds. The aim of this study was to investigate the efficacy of green betel leaf extract cream in the healing of cut wounds on the skin of white Wistar rats. The design employed was a pre-test post-test with a control group design and a sample size of 20 white rats divided into four groups. Betel leaf extract was prepared using the maceration method, while a cream preparation of betel leaf extract was prepared using the oil-in-water emulsion type (M/A). Macroscopic histopathological evaluation was performed by examining fibroblast growth. A one-way analysis of variance (ANOVA) test (p < 0.05) was used to determine the difference between groups, and a post hoc least significant difference (LSD) test was used to analyze which treatment group had the greatest efficacy. Technical terms were explained upon first use, and the language used was clear, objective, and value-neutral to improve academic quality. The text was free from grammatical errors, spelling mistakes, and unnecessary jargon. A one-way analysis of variance (ANOVA) test (p < 0.05) was used to determine the difference between groups, and a post hoc least significant difference (LSD) test was used to analyze which treatment group had the greatest efficacy. The study revealed a substantial difference in the average wound healing rates between the control group (P0) and treatment groups P1, P2, and P3. This is due to the lack of active substances in the control group (P0), which aids in accelerating the wound healing process. Notably, the 15% green betel leaf extract cream demonstrated superior efficacy in healing wounds in white rats compared to the 10% and 25% green betel leaf extract creams. The results indicated no significant difference between the application of 15% and 25% green betel leaf extract cream. This is due to the fact that secondary metabolite compounds present in the extract produce a healing effect on wounds at these particular levels. However, lower concentrations only act as microorganism inhibitors, rendering them less effective for promoting wound healing.

Anticancer Activity of Rosella Flowers (Hibiscus Sabdariffa l.) In HepG2 cells

The natural ingredients of rosella flowers have the potential as natural anti-cancer and chemopreventive agents, contain secondary metabolite compounds with cancer therapeutic mechanisms with inhibiting and killing cancer cells and their development through inhibition of apoptosis (cell death program) and in vitro inhibited cell flow. The study aims to identification of secondary metabolite compounds and also determine the cytotoxic activity of ethanol extract from the rosemary plant against HepG2 cancer cells. This study used by identification of secondary metabolite compounds of ethanol extract from the rosemary plant (Hibiscus sabdariffa L.) and the cytotoxicity test used with MTT assay on HepG2 liver cancer cells with a test concentration series of 12,5; 25; 50; 100; 200; 250; 500 and 1000 µg/ml. : The results showed the secondary metabolite compounds of rosella flowers have flavonoids, alkaloids, saponins and tannins and data of 50% cell growth inhibition activity, which was showed that rosella flower had IC50 values 629,919 μg/mL. The ethanol extract of rosella flowers have activity on HepG2 cells growth inhibition had considered moderately active anticancer activity

AKTIVITAS ANTIJAMUR EKSTRAK ETANOL DAUN KRATOM (Mitragyna speciosa) TERHADAP JAMUR Microsporum canis

The world population experiencing dermatophytosis has reached 20%. Dermatophytosis is an infection caused by dermatophyte fungi. The prevalence in Asia reaches 35.6%. One of the fungi that causes dermatophytosis is Microsporum canis. Microsporum canis is known to be resistant to antifungal drugs. Kratom (Mitragyna speciosa) is a plant originating from Southeast Asia and in Indonesia it is often found in the Kapuas Hulu area, West Kalimantan. Kratom leaves are widely used as herbal medicine and have secondary metabolite compounds that have the potential to be used as antifungals. This study aims to determine the antifungal activity of ethanol extract of kratom leaves (Mitragyna speciosa) on the growth of Microsporum canis. Phytochemical screening was carried out using the Thin Layer Chromatography (TLC) scanner method and spray testing to determine the secondary metabolite compounds of the extract being tested. The test extract was made in concentrations of 5%, 10%, 15%, 20%, 25%, 50%, 75%, and 90% (w/v) with ethanol as a solvent. 8 µg itraconazole discs were used as a positive control and 10% DMSO was used as a negative control. Antifungal activity testing was carried out using the disc diffusion method by measuring the fungal growth inhibition zone. The ethanol extract of kratom leaves contains secondary metabolite compounds, namely alkaloids, terpenoids, steroids, flavonoids and tannins with the most dominant groups of alkaloid and tannin compounds. The results showed that there was no growth inhibition zone for Microsporum canis at all extract concentrations tested. The ethanol extract of kratom leaves does not have antifungal activity against the growth of Microsporum canis.

Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility

Globally, 48 million couples experience infertility, where male infertility factors contribute to 50% of cases. Spermatozoa motility is a crucial parameter in assessing male fertility. Antioxidants act as the body's defence against excessive ROS and can be used as a treatment for male infertility. One of the local plants in Central Kalimantan that is potentially rich in antioxidants is Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb. However, there is limited research on the bioactivity of Bajakah Kalalawit on spermatozoa in vitro. This study aims to determine the effect of ethanol extract of Uncaria gambir (W. Hunter) Roxb on spermatozoa motility in vitro. Bajakah Kalalawit stems were extracted using a 3x24-hour maceration technique with 96% ethanol. After obtaining the concentrated extract, the secondary metabolite compound content was analyzed through a phytochemical screening. The sample used was in vitro human spermatozoa that were washed and added to Bigger Whitten Whittingham medium, then incubated at 37°C for 1 hour with a 96% ethanol extract of Bajakah Kalalawit at doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml, as well as a control group (Bigger, Whitten & Whittingham medium only). The results showed that the compounds contained in the ethanol extract of Uncaria gambir (W.Hunter) Roxb were terpenoids, flavonoids, phenolics, steroids, saponins, alkaloids, and tannins. Spermatozoa motility significantly increased in the treatment groups starting from doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml compared to the control group. Ethanol extract of Uncaria gambir (W.Hunter) Roxb could increase spermatozoa motility in vitro and succeeded in improving reproductive technology.

Potential Use of Papaya Flower Extract (Carica papaya Linn) for Aedes Aegypti Larvicide

Introduction: Temefos as a larvicide to prevent dengue hemorrhagic fever is known to be resistant to Aedes aegypti larvae in several regions in Indonesia. Therefore, alternative larvicides are needed. Papaya plants are one of the alternative solutions as papaya flowers contain secondary metabolite compounds that can kill Aedes aegypti larvae. This study aimed to determine the potential of papaya flower extract to be an Aedes aegypti larvicide.Methods : This study was a true experimental study with a posttest-only control group design, with 6 treatment groups namely 1%, 5%, 10%, 15%, and 20% extract concentrations (Experimental Group) and distilled water (Control Group). The experiment was repeated 4 times with a testing time of 24 hours. A total 600 Aedes aegypti instar larvae III/IV were used. Larvae toxicity was analyzed using Probit Analysis to determine the value of Lethal Concentration (LC) and Lethal Time (LT) and the Kruskall-Wallis test to determine the differences of larval deaths in each experimental group. Results: Results showed that the highest and the lowest average larval mortality was at 20% concentration (25 larvae) and 1% concentration (14.75 larvae), respectively. and values were 4.84% and 10.39%, while values of five concentrations were 115.74, 24.24, 23.41, 25.24, and 16.55 hours and values of five concentrations were 241.95, 29.42, 26.20, 32.24, and 24.48 hours. There was a difference in the number of larval deaths after 24 hours of treatment in the experimental group (p-value = 0.011).Conclusion: Papaya flower extract has the potential to be an Aedes aegypti larvicide with 20% concentration as the optimum dose. However, phytochemical screening needs to be done to determine the dominant papaya flower compounds act as Aedes aegypti larvicides.

Identification of Secondary Metabolite Compounds from Melandean (Bridelian micrantha) Leaf Extract

Identification of Secondary Metabolite Compounds from Melandean (Bridelian micrantha) Leaf Extract

Alzheimer's Disease-Related Proteins Targeted by Secondary Metabolite Compounds from Streptomyces: A Scoping Review.

Alzheimer's disease (AD) is a neurodegenerative disease that is characterized as rapid and progressive cognitive decline affecting 26 million people worldwide. Although immunotherapies are ideal, its clinical safety and effectiveness are controversial, hence, treatments are still reliant on symptomatic medications. Concurrently, the Streptomyces genus has attracted attention given its pharmaceutically beneficial secondary metabolites to treat neurodegenerative diseases. To present secondary metabolites from Streptomyces sp. with regulatory effects on proteins and identified prospective target proteins for AD treatment. Research articles published between 2010 and 2021 were collected from five databases and 83 relevant research articles were identified. Post-screening, only 12 research articles on AD-related proteins were selected for further review. Bioinformatics analyses were performed through the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) network, PANTHER Go-Slim classification system (PANTHER17.0), and Kyoto Encyclopedia of Genes and Genomes (KEGG) Mapper. A total of 20 target proteins were identified from the 12 shortlisted articles. Amyloid-β, BACE1, Nrf-2, Beclin-1, and ATG5 were identified as the potential target proteins, given their role in initiating AD, mitigating neuroinflammation, and autophagy. Besides, 10 compounds from Streptomyces sp., including rapamycin, alborixin, enterocin, bonnevillamides D and E, caniferolide A, anhydroexfoliamycin, rhizolutin, streptocyclinone A and B, were identified to exhibit considerable regulatory effects on these target proteins. The review highlights several prospective target proteins that can be regulated through treatments with Streptomyces sp. compounds to prevent AD's early stages and progression. Further identification of Streptomyces sp. compounds with potential anti-AD properties is recommended.

Potential of gambier leaf ethanol extract cream for incised wound healing

Incised wounds are highly susceptible to bacterial infection, which requires stimulation of healing and restoration of normal function of the injured body part. This study aimed to test the effect of gambier leaf ethanol extract cream administration on the acceleration of wound healing on the skin surface of male Wistar rats. The research used a pre-test post-test with a control group design. Twenty male white rats were divided into four groups: control group (P0), treatment group 1 (P1), treatment 2 (P2), and treatment 3 (P3). The incision wounds in white rats were treated twice a day (in the morning and evening) for 14 days. Wound healing was observed by measuring the average length of the wound every day, from the first day of wounding until day 14. Statistical tests used were one-way ANOVA and post-hoc LSD test (p < 0.05). In this study, it was found that the 15% gambier leaf extract cream was more effective in wound healing in white rats than the 5% and 7.5% gambier leaf extract creams. However, in the group treated with 7.5% gambier leaf extract cream, the situation was already close to that in the treatment group treated with 15% gambier leaf extract cream. This is because, at a concentration of 7.5%, the secondary metabolite compounds in the gambier leaf extract had an effect on wounds, but at a concentration of 15% gambier leaf extract cream, the effect was similar to that of 15% gambier leaf extract cream.

GC-MS analysis and cytotoxic activity of the n-hexane fraction from Curcuma sahuynhensis Škornick. & N.S.Lý leaves collected in Vietnam

Curcuma sahuynhensis Škornick. & N.S.Lý is an endemic plant in Vietnam that has been used by the Sa Huynh people as a spice and medicine to cure illnesses linked to digestive disorders. Very little information is available so far about the chemical composition and biological effects of C. sahuynhensis. To find new pharmaceutical ingredients, the in vitro cytotoxic effect and the chemical profile of C. sahuynhensis leaf extract were investigated. In this study, the percolation method and liquid-liquid dispersion technique were used to extract dry sample powder. The chemical composition was detected by gas chromatography-mass spectrometry (GC-MS). The Sulforhodamine B and MTT methods were used to determine the cytotoxic activity. The chemical composition analysis showed that the leaf extract contained 14 components. The major components in the n-hexane extract were 6,10,14-trimethylpentadecan-2-one, phytol, 1-ethylbutyl hydroperoxide, isoborneol, 1-methylpentyl hydroperoxide, and neophytadiene. On human cancer cell lines, namely MFC-7, SK-LU-1, Hela, MKN-7, and HL-60, the leaf extract showed dose-dependent cytotoxic activity, with IC50 values ranging from 221.70±10.24 to 369.42±10.60 ?g/mL. The present study provides significant information on the chemical components and cytotoxic effects of the n-hexane extract from C. sahuynhensis leaves. The findings will continue to be crucial in future research on the evaluation of secondary metabolite compound analysis for cancer therapeutic effects.

Bioactivity mapping of secondary metabolite compounds of Pandanus amaryllifolius leaves as anti-inflammatory using in silico

Inflammation is one of the primary responses of the immune system to infection and irritation. Anti-inflammatory drugs generally cause side effects. Anti-inflammatory developed by reducing side effects use many natural materials such as plants. The parts of the plant used include fruits, leaves, stem bark, rhizomes, and flowers. One of the plants that can be used as an anti-inflammatory is Pandanus amaryllifolius. P. amaryllifolius leaves contain several materials, such as flavonoids, alkaloids, saponins, tannins, polyphenols, and dyes. This study aimed to determine the anti-inflammatory potential of the secondary metabolites of Pandanus amaryllifolius leaves using the in silico method. The research used a descriptive exploratory method and was conducted from December 2022 – January 2023. In silico mapping of the bioactivity of active compounds was carried out using several software or websites: knapsack database (www.knapsackfamily.com), NCBI PubChem database (https://pubchem.ncbi.nlm.nih.gov/), PASS Online Way 2 Drug (http://www.way2drug.com/passonline/) and ADME Swiss Analysis (http://www.swissadme.ch/). The result shows P. amarylifolius has 31 active compounds. The compounds were then analyzed using Pass Online with 18 anti-inflammatory parameters. It explained that 3 compounds met the rules for Pa values 0.7, namely compounds 6E-Pandanamine (0.758), Pandamenyamine (0.735), and Pandamarilactone 1 (0.709). The results of pharmacokinetic tests using Lipinski

Phytochemical analysis of secondary metabolite compounds of Pandanwangi leaf extract (Pandanus amaryllifolius)

The majority of people use pandan as a dye, food fragrance, and natural medicine because it contains secondary metabolites such as alkaloids, saponins, and phenolics. Therefore, this research aims to explore the secondary metabolites of Pandanus amaryllifolius using qualitative and quantitative methods. The research begins by taking samples of pandan leaves, which are then extracted and tested qualitatively and quantitatively. The qualitative test results showed that pandan leaf extract contained flavonoids, polyphenols, and tannins. In the quantitative test of pandan leaf phenolic compounds using a UV-Vis spectrometer with gallic acid concentrations of 80 ppm, 100 ppm, 120 ppm, and 140 ppm, Based on the quantitative test, the total phenolic content of pandan extract is 114 mg/L.