Second-intention Wound Healing Research Articles

The effects of a synthetic epidermis spray on secondary intention wound healing in adult horses.

To evaluate secondary intention wound healing in the horse's limbs when treated with the synthetic epidermis spray (Novacika®, Cohesive S.A.S, France) or with a standard bandaging technique. Six Standardbred mares were included in the study. Four 2.5 x 2.5 cm full-thickness skin wounds were created on each thoracic limb. Two wounds were located on the dorsoproximal aspect of the cannon bone and the other two at the dorsoproximal aspect of the fetlock. Six hours after creation, wounds were randomly treated with synthetic epidermis spray or standard bandaging. The wounds were assessed every 4 days by gross visual assessment and using a 3D imaging camera. Analysis was performed with a 3D imaging application. Out of 46 wounds, 22 showed exuberant granulation tissue and were part of the standard bandaging group. Whether the wounds were treated with synthetic epidermis spray or standard bandaging, the time for healing was the same. The synthetic epidermis spray studied in this model has allowed healing without the production of exuberant granulation tissue but did not reduce the median wound healing time compared to a standard bandaging technique. The synthetic epidermis spray is potentially an interesting alternative for the management of secondary intention wound healing of superficial and non-infected distal limb wounds in adult horses on economical and practical aspects. However, all statistical inference (p-values especially) must be interpreted with caution, given the size of the sample.

Evaluation of the Effectiveness of Medical-Grade Honey and Hypericum Perforatum Ointment on Second-Intention Healing of Full-Thickness Skin Wounds in Cats.

This study aimed to determine the effects of two topical treatments on second-intention wound healing in cats. Eight 2 × 2 cm full-thickness wounds were created, four on each side of the dorsal midline of eight laboratory cats, to receive either medical-grade honey ointment (MGH) and its control (HC), or Hypericum-based ointment (HP) and its control (HPC). MGH or HP ointment was applied to four wounds on the same side, while the remaining four were used as controls, chosen at random. Planimetry, laser Doppler flowmetry, daily physical examinations, and histologic examinations on days 0, 7, 14, and 25 were used to assess the healing of wounds. Tissue perfusion was better in the MGH-treated (2.14 ± 0.18 mm/s) and HP-treated wounds (2.02 ± 0.13 mm/s) than in the untreated controls HC (1.59 ± 0.11 mm/s) and HPC (1.60 ± 0.05 mm/s), respectively (p = 0.001). Histopathology revealed that the median edema score was lower in the MGH-treated (2; range 1-4) compared to the HC-treated wounds (3; range 2-4) on day 7 (p < 0.05). The median angiogenesis score was higher on day 7 in the MGH-treated (2; range 1-3) compared to the HP-treated wounds (2; range 1-2) (p = 0.046). The fibroblast concentration was increased in the MGH-treated wounds (3.5; range 3-4) compared to the HP-treated wounds (3; range 2-4) on day 25 (p = 0.046). MGH and HP increased tissue perfusion compared to the untreated controls. The MGH-treated wounds had histologic parameters superior to the HP-treated wounds regarding angiogenesis and fibroblast concentration in cutaneous wound healing in cats. Topical application of MGH and HP did not accelerate the healing process of feline cutaneous wounds.

Clinical Evaluation of Medical Ozone Use in Domestic Feline Cutaneous Wounds-A Short Case Series.

Support and management of second-intention wound healing involves frequent dressing changes having different properties. Dressings can range from simple ones, such as nonadherent dressings, to more complex options, such as foam, hydrocolloid, alginate or negative pressure dressings. Seven cats were enrolled in the study with a total of nine wounds of various sizes with different etiology sizes and localizations. Three methods of local ozone administration were used to cover more of the ozone properties in the treatment of wounds: bagging, perilesional subcutaneous infiltrations and lavages with ozonated saline. Evaluation of the healing process was performed by clinical observation and wound area measurements every seven days until the complete recovery of the patients. The results of this study should encourage clinicians to consider medical ozone as a new therapeutic approach with regenerative properties in the second-intention healing of cats presenting cutaneous wounds.

Clinical and histological comparison of healing by steel scalpel, diode laser, and radiofrequency in palatal wound: An animal study.

Wound healing following periodontal soft tissue procedures can differ owing to different techniques, the feasibility of which can be determined through detailed macroscopic and microscopic observations. This study aimed to clinically and histologically evaluate palatal wound healing in rats by secondary intention after excision using a steel scalpel, diode laser, and radiofrequency. An excision was made in the edentulous anterior maxilla of 42 4-month-old male Wistar rats weighing 289-428 g. Part of the connective tissue was left in the surgical area to observe the dynamics of secondary intention wound healing. Three experimental groups were established: the steel scalpel, an 810-nm diode laser at a power output of 1.5 W in continuous mode, and a monopolar radiofrequency in a fully rectified waveform at 15 W. Clinical and histological analyses were performed on days 2, 4, and 7. Hemostasis, changes in body weight, defect size, epithelial gap, and inflammatory infiltration were evaluated. The epithelial gap closed completely in all groups on day 7. Bleeding occurred significantly more in the scalpel group (P < 0.001). No significant changes were observed in body weight between the groups. Macroscopically, the mean wound area decreased over time in all groups. Wound healing was significantly slower in the laser group on day 2 and in the radiofrequency group on days 4 and 7 (P < 0.001). Microscopically, the laser created the cleanest wound area, with minimal inflammatory infiltration and no thermal injury. More damage occurred in the connective tissue of the radiofrequency group. Wound healing was observed on day 7 in all groups. Palatal wound healing with secondary intention yielded different outcomes in a rat model when different techniques were used. However, almost complete healing was observed in all wounds, which highlights the importance of the soft tissue left in the surgical area. Wound healing in periodontal soft tissue procedures is not compromised by different techniques, as long as the clinician has sufficient knowledge and experience.

Correction of Habit along with Laser Assisted Frenectomy in High Frenal Attachment - A Case Report

Digit sucking is the most prevalent oral fixation seen in young toddlers. Digit sucking can be brought on by fatigue, boredom, excitement, hunger, fear, and both mental and physical stress. If these practices persist until the preschool years, when permanent teeth start to erupt, they may operate as a substantial environmental etiological factor in the development of malocclusion. The diode laser can be used to successfully remove pathological frenum. The diode laser can be used in paediatric dentistry due to its application, adequate coagulation, lack of need for sutures, and decreased discomfort and inflammation. In the case study presented in this paper, an 8-year-old female patient who had a high frenal attachment and had thumb-sucked her entire life was treated with fixed appliance therapy and laser labial frenectomy. In addition to resolved malocclusion, acceptable secondary intention wound healing, and beginning functional recovery of a physiological upper lip movement, the clinical evaluation revealed that the malocclusion had been corrected. The surgery was well tolerated, according to the patient. Keywords: Thumb sucking, Fixed appliance therapy, Laser frenectomy

Carotid EndoVAC: a novel hybrid technique for carotid Dacron patch infection

This paper reports the use of the EndoVAC technique in a case of left carotid artery Dacron patch infection. The EndoVAC technique involves a sequence of endovascular relining with a stent graft, surgical debridement with explantation of infected graft material and secondary intention wound healing with a vacuum-assisted closure (VAC) device and long-term antibiotic treatment. Our patient was considered a high-risk surgical candidate due to previous cranial nerve injury, high carotid stent graft positioned in zone 3 of the neck, and high stroke risk due to aberrant cerebrovascular anatomy. The carotid EndoVAC technique was performed without complication and a successful outcome was observed at 1-year follow-up. Although currently not recommended as first-line management in treating vascular graft and endograft infections of the carotid vessels, the EndoVAC approach should be considered in a select cohort of patients when neither traditional radical surgery nor conservative simple VAC therapy are considered feasible or safe.

Association Between Wound Healing and Psycho-Emotional State Among Postoperative Patients

Wound healing is a difficult process, it can be complicated by experiences that can occur during pre-operative and post-operative periods. Emotions have a substantial influence on human behaviour and health. Therefore, it is important to study interaction of wound healing and psychoemotional state, in order to obtain efficient wound treatment and care in Lithuania.&#x0D; Aim of the work - to evaluate interface of postoperative patients of wound healing and psycho-emotional state.&#x0D; Methods. A mixed study was conducted, combining quantitative and qualitative research methods. Instruments used: questionnaire (Minnesota Multiphasic Personality Inventory (MMPI-2) test and the Lüscher color test) and medical document analysis (used to identify wound healing type). Statistical analysis of research data performed using statistical software package SPSS 17.0 and Microsoft Excel 2016. Research sample – 383 respondents. Research conducted in accordance with the principle of ethics.&#x0D; Results. The study revealed that women reported higher levels of post-operative state anxiety. Men tend to value their psycho-emotional stress levels more favourably. Also, data analysis revealed that there was an increase in men interaction with environment during post-operative period. Female respondent group showed statistically significant dependence between concomitant illnesses and wound healing, when concomitant illnesses affected wound healing in a negative way. There was a dominance of secondary intention wound healing in respondents (male and female) who had higher levels of post-operative psycho-emotional stress.&#x0D; Conclusions. Taken together, findings indicate that negative psycho-emotional experiences during post-operative period affect wound healing in a negative way, slowing down the healing process.

Effect of Canine Adipose Mesenchymal Stem Cell Secretome on a Model of Second-Intention Wound Healing in the Red-Eared Slider Turtle (Trachemys scripta).

Mesenchymal stem cell (MSC) secretome refers to a variety of bioactive compounds that represents the more important pathway by which MSCs participate in tissue regeneration. Many of these compounds have shown variable functional activity even across nonmammalian vertebrate species, although MSCs in turtles have not yet been described. Canine adipose MSC secretome has been successfully used experimentally in skin healing. Our aim was to conduct a blinded controlled study to evaluate the effect of canine adipose MSC secretome (cS-MSC) as an alternative for the healing of soft skin, second intention wounds of red-eared slider turtles (Trachemys scripta). Under general anesthesia, one circular, 6-mm full thickness wound was made in each rear leg of 14 females. After randomization, cS-MSC was injected subcutaneously around one wound at days 1, 7, and 14, whereas the other wound acted as control. Biopsies from three animals' wounds were obtained at days 21, 28, 42, and 63. Differences in mean wound retraction at days 21 (n=14) and 28 (n=11) were statistically nonsignificant. The clinical and histopathologic scores performed blind by two different investigators were similar for treated and control wounds. In conclusion, we could not detect a significant functional activity of cS-MSC on wound healing of Trachemys scripta.

Topical timolol enhances surgical wound healing in the lower portion of the leg in older patients with comorbidities: A retrospective review

Topical timolol enhances surgical wound healing in the lower portion of the leg in older patients with comorbidities: A retrospective review

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator.

The process of secondary intention wound healing includes long repair and healing time. Electrospun nanofibrous scaffolds have shown potential for wound dressing. Biopolymers have gained much attention due to their remarkable characteristics such as biodegradability, biocompatibility, non-immunogenicity and nontoxicity. This study anticipated to develop a new composite metronidazole (MTZ) immobilized nanofibrous scaffold based on poly (3-hydroxy butyrate) (PHB) and Gelatin (Gel) to be utilized as a novel secondary intention wound healing accelerator. Herein, PHB and Gel were mixed together at different weight ratios to prepare polymer solutions with final concentration of (7%), loaded with two different concentrations 5% (Z1) and 10% (Z2) of MTZ. Nanofibrous scaffolds were obtained by manipulating electrospinning technique. The properties of MTZ immobilized PHB/Gel nanofibrous scaffold were evaluated (SEM, FTIR, TGA, water uptake, contact angle, porosity, mechanical properties and antibacterial activity). Additionally, in vitro cytocompatibility of the obtained nanofibrous scaffolds were assessed by using the cell counting kit-8 (CCK-8 assay). Moreover, in vivo wound healing experiments revealed that the prepared nanofibrous scaffold highly augmented the transforming growth factor (TGF-β) signaling pathway, moderately suppressed the pro-inflammatory cytokine (IL-6). These results indicate that MTZ immobilized PHB/Gel nanofibrous scaffold significantly boost accelerating secondary intention wound healing.

The role of alginate dressings in wound healing and quality of life after pilonidal sinus resection: A randomised controlled trial.

In this trial, we evaluated the role of alginate dressings in the secondary intention wound healing and quality of life (QoL) after pilonidal sinus resection. The study was designed as a prospective randomised controlled trial (RCT). In the experimental group, alginate dressings with silver and high‐G cellulose were introduced after elective pilonidal cyst excision, whereas in the control group, simple gauges were used. The primary end point was the difference in terms of the wound healing period. Blinding existed at the level of the investigator. Overall, 65 patients were included during the study period. Wound healing duration was comparable between the two groups (P = .381). No difference in postoperative pain scores or recovery outcomes was found. The experimental group was associated with reduced wound secretions at specific time end points. Similarly, no effect was identified, on overall Wound‐QoL or SF‐36 scores. Alginate dressings do not accelerate wound healing or improve QoL. Due to suboptimal sample size and several study limitations, further RCTs are required to confirm our findings.

INFLUENCE OF 627 NM WAVELENGTH LIGHT EMITTING DIODE PHOTOTHERAPY ON SECONDARY INTENTION WOUND HEALING

Background: The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Was evaluated the effect of 627 nm wavelength LED irradiation on VEGF-α and TGF-β1 expressions in a model of secondary intention wound healing. Methods: The evaluations were performed using a dorsal puncture model and the sample consisted of 120 Wistar rats, randomly divided into LED groups submitted to 3, 7, 14 and 21 irradiation treatment for 100 s, 7 J / cm2, and control groups. Histological analysis, immunohistochemistry and contraction of the lesion were performed. Results: LED irradiation led to effective wound regeneration results. Similar effects were obtained with vascular, exudative, and proliferative phenomena. VEGF and TGF-β1 levels were increased in LED-treated groups with initial treatments. Collagen analysis revealed that LED-treated groups levels were increased on days 14- and 21 of the treatments. An acceleration in tissue regeneration was observed in LED treated groups. Macroscopically visible results showing a complete healing process was evident after 14 days of treatment. Conclusion: 627 nm LED irradiation effectively accelerated wound healing leading complete closure of the skin in a shorter time compared to control group.

Using Dry Human Amniotic Membrane in Secondary Intention Wound Healing After Urological Cancer Surgery: The First Randomized Clinical Trial in Iran

Background: Wound healing is a complex process and in some patients, it is a long process. Due to the presence of various underlying diseases in patients with cancer, this process is delayed, and as a result, the rate of complications increases. Therefore, some materials are needed to accelerate wound healing. Nowadays efficacy of dry human amniotic membrane in burn wounds and chronic wounds is proven; however, no studies have been performed on the effect of this tissue in the treatment of surgical wounds. Objectives: This study aimed at evaluating the efficacy of dry human amniotic membrane in the secondary repair of urological cancer surgery wounds. Methods: In this randomized clinical trial from January 2018 to June 2020, 40 patients who underwent urological cancer surgery and secondary intention wound healing were selected and randomly divided into 2 groups of 20 patients. In the case group, the dry human amniotic membrane was used for dressing wounds, and in the control group, normal dressing without an amniotic membrane was performed. Complications such as infection, sepsis, and reoperation, as well as the duration of wound healing and hospitalization of patients at intervals of 1 week, 1, and 3 months, were compared between the 2 groups. Results: The 2 groups matched in terms of gender, age, and wound location. The mean duration of hospitalization and wound healing was significantly lower in the case group (P &lt; 0.05). The rate of complications was higher in the control group but was not significant. Conclusions: Dry human amniotic membranes due to the accelerated wound healing process and fewer complications could be a good choice for secondary healing of wounds after urological cancer surgery.

Effect of photobiomodulation in secondary intention gingival wound healing\u2014a systematic review and meta-analysis

BackgroundPhotobiomodulation is widely being used to improve the wound healing process in dentistry and a vast majority of studies have proven its benefits. But there are plenty of knowledge gaps according to the optimal laser characteristics which should be used to maximize the healing effects of lasers. The goal of this systematic review and meta-analysis was to determine the effect of photobiomodulation (PBM) as an adjunctive treatment to periodontal therapies to evaluate secondary intention gingival wound healing and post-operative pain.MethodsFive databases (PubMed, Embase, Scopus, ProQuest, and Web of Sciences) were searched up to November 30, 2020, for clinical trials that reported the result of the application of PBM on secondary gingival healing wounds and post-operative pain and discomfort after periodontal surgeries. Two independent reviewers selected the eligible studies and the outcomes of interest were extracted. The quality of eligible studies was assessed using the Cochrane Handbook for Systematic Reviews of Interventions.ResultsUltimately, twelve studies were included in this review. The application of PBM as an adjunct to periodontal surgeries resulted in a significant improvement in wound healing indices. The Landry wound healing index at the 7th post-operative day was significantly improved (SMD = 1.044 [95% CI 0.62–1.46]; p < 0.01) in PBM + surgery groups compared to the control groups. There was also a statistically significant increase in the complete wound epithelialization (RR = 3.23 [95% CI 1.66–6.31]; p < 0.01) at the 14th post-operative day compared to the control groups. The methods used to assess the post-operative pain were heterogeneous, and therefore the results were limited which made the meta-analysis for post-operative pain assessment not possible.ConclusionBased on the results of this review, PBM can be effectively used as a method to improve secondary intention wound healing. High-quality randomized clinical trials, however, are needed in the future to identify the optimal PBM irradiation parameters and the effect of PBM on post-operative pain.

Therapeutic Targeting of Neutrophil Extracellular Traps Improves Primary and Secondary Intention Wound Healing in Mice.

BackgroundNeutrophils are the first responders in wound healing after injury that mediate pro- and anti-inflammatory activities i.a. through the formation of extracellular traps (NETs). However, excessive NETs presence in wound tissue can cause local hyperinflammation and -coagulation resulting in delayed wound healing. To improve wound healing, we aimed to examine the role of NETs and DNase1 on primary and secondary wound healing.MethodsThe study included 93 C57BL/6 mice, with 3 different genotypes: wildtype, Pad4-, and DNase1-Knockout (KO). Pad4-KO mice show limited NETs formation, while DNase1-KO mice cannot disintegrate them. All 3 genotypes were included in (1) a laparotomy group and (2) a thermal injury group. Animals in both groups either received DNase1 or a vehicle i.p. post wound induction and wound assessment and euthanasia were conducted. Laparotomy and burn scars were assessed using the stony brook scar evaluation scale and modified Yeong scale respectively. Tissue was analyzed histologically using H&E staining. Ly6g, Collagen I and III, SMA, and Fibrinogen were visualized and neutrophils activation (NE, MPO) and NETs (H3cit) formation assessed.ResultsAll animals survived with no complications. DNase1 treatment led to a significantly improved scar appearance in both groups, which was also seen in Pad4-KO mice. In the laparotomy group DNase1 improved collagen deposition and fibrin concentration was significantly reduced by DNase1 treatment. Markers of neutrophil activation were significantly reduced in the treatment and Pad4-KO group. In the thermal injury group wound closure time was significantly reduced after DNase1 treatment and in the Pad4-KO group. Even though inflammation remained high in the thermal injury model over time, neutrophil activation and NETs formation were significantly reduced by DNase1 treatment compared to controls.DiscussionPrimary and secondary intention wound healing is improved by targeting NETs through DNase1 treatment or genetic KO, as assessed by wound closure time and scar appearances. Additionally, wound stability was not affected by DNASE treatment. The results suggest that overall wound healing is accelerated and DNase1 appears to be a promising option to reduce scar formation; which should be evaluated in humans.

Use of platelet concentrate gel in second-intention wound healing: a case\xa0report

BackgroundWound healing is a complex and dynamic process. Healing of acute and chronic wounds can be impaired by patient factors (that is, comorbidities) and/or wound factors (that is, infection). Regenerative medicine products, such as autologous/homologous platelet-rich plasma gel, may speed up the healing process. Autologous/homologous platelet-rich plasma is an advanced wound therapy used for hard-to-heal acute and chronic wounds. The cytokines and growth factors contained in platelet-rich plasma play a crucial role in the healing process.Case presentationA 61-year-old Caucasian male patient, suffering from mental retardation following meningitis, with a transplanted kidney due to prior renal impairment, and under immunosuppressant therapy, was submitted to aneurysmectomy of his proximal left forearm arteriovenous fistula. A few days later, the patient came to our attention with substantial blood loss from the surgical site. The wound presented no signs of healing, and after fistula reparation and considering persistent infection of the surgical site (by methicillin-resistant Staphylococcus aureus), surgeons decided for second-intention healing. To favor healing, 10 mL homologous platelet concentrate gel was sequentially applied. After each application, wound was covered with nonadherent antiseptic dressing. After only seven applications of homologous platelet concentrate gel, wound completely recovered and no amputation was necessary.ConclusionsTopical application of homologous platelet-rich plasma gel in healing wound shows beneficial results in wound size reduction and induces granulation tissue formation. Platelet-rich plasma could be a safe and cost-effective treatment for managing the cutaneous wound healing process to shorten the recovery period and thereby improve patient quality of life.

Photodynamic Therapy Delays Cutaneous Wound Healing in Mice

Cutaneous wound healing is a complex, dynamic physiological process. Traditional methods of promoting wound healing are not always effective. Consequently, alternative modalities, such as photodynamic therapy (PDT), are needed. We examined the effectiveness and underlying mechanisms of PDT in a murine model of acute wound healing. Two excisional wounds were produced, one on each side of the midline, in C57bL/6J mice. Methyl 5-aminolevulinate hydrochloride (MAL) was applied to the right-side wound. After 1 to 3 hours of incubation, the wound was irradiated with red light. The left-side wound was not treated with MAL or red light. On Day 14, the wounds were excised and subjected to histological and immunohistochemical analysis. During the first week, no difference was seen between the two sides. However, at week 2, PDT-treated wounds exhibited delayed re-epithelialization. On Day 14, hematoxylin and eosin (HE) staining showed a continuous epithelial lining in untreated wounds. In contrast, PDT-treated wounds partially lacked epithelium in the wound bed. Masson's Trichrome (MTC) staining showed a thicker dermis and more collagen fibers and inflammatory cells in PDT-treated wounds than in untreated wounds. Immunohistochemical analyses showed significantly fewer CD31+ blood vessels and greater collagen III density in PDT-treated wounds than in untreated wounds. However, treated and untreated wounds did not differ in collagen I density. PDT delayed acute wound healing in a murine model of secondary intention wound healing.

Glycyrrhizinate Dipotassium (DPG) Effects over Skin Wound Healing

Skin wound healing is a complex process involving cellular, and molecular events: inflammatory, proliferative, and remodeling phases. Infectious disease, pre‐existing diseases, medications, and aging can negatively interfere in healing. Research on leptin, collagenase, and tretinoin have been done attempting to promote healing. Glycyrrhizinate Dipotassium (DPG), derived from licorice, has anti‐allergic, antibiotic, and anti‐inflammatory effects, besides promoting the skeletal muscle regeneration by activating quiescent satellite cells, leading myoblasts and myotubes differentiation, and forming new muscle fibers. Considering these aspects, we purpose to verify the 2% DPG pharmacological effect on secondary intention wound healing in adult and aged animals. Twenty male Wistar rats were used. Two skins' excisions (2 cm diameter) of normal skin were made in the animal dorsal region (Naïve group). Topical application of 2% DPG was done on the distal wound daily for 7 days (Treated group). The proximal wound was not treated (Control group). After 14 or 21 days (n = 5 each) of wound creation, the samples were excised, fixed in 10% formol, and processed for paraffin inclusion. Slides were stained with hematoxylin and eosin (H&amp;E), and Masson's trichrome (MT) for histological analysis. Wound healing areas, epidermal and dermal measurements were performed. Statistical analysis adopted a 5% coefficient for null hypothesis rejection. Histological analysis revealed intact skins for Naïve group. Control and Treated groups presented skin with epidermis and dermis reshaped: all epidermal layers (basale, spinosum, granulosum, lucidum, and corneum stratum), as papillary and reticular dermis. In aged rats, the wound was smaller in treated than in untreated animals, resulting in a larger scar compared with the adult rats. However, when treated wounds were compared, no differences were found between the wound areas in adult and aged rats. After 14 days the epidermis thickness of adult and aged animals treated with DPG was thicker than untreated animals. However, after 21 days, the adult epidermis thickness of animals treated with DPG was thinner than untreated animals. The dermis thickness of adult and aged animals treated or non‐treated with 2% DPG did not differ. On the 21st day, adult and aged complete reepithelialization were found in treated animals. Results indicate that 2% DPG significantly reverses wounds process to a skin normal state, even for aged animals, by reepithelization, probably acting during inflammatory and proliferative phases of healing. Such results point DPG as a promissory agent to promote skin healing.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Assessment of Palatal Mucosal Wound Healing Following Connective-Tissue Harvesting by Laser Speckle Contrast Imaging: An Observational Case Series Study.

Postoperative complications may occur during the healing of palatal donor sites due to disturbed blood circulation of palatal tissues. Therefore in this study, blood flow was measured by Laser Speckle Contrast Imaging (LSCI) in seven patients after connective-tissue harvesting. The slope in blood-flow elevation within the first 3 days as well as time needed for maximum reperfusion were calculated. Each surgical site was assessed by clinical examination on day 3. In donor sites with secondary-intention wound healing, postoperative blood flow was elevated with significant delay compared to the surrounding tissues and to the primarily healed wound. Reperfusion time and healing score were strongly correlated (r = 0.87, P < .001), as were the slope and clinical rank (r = -0.85, P < .001). LSCI proved to be an objective method to assess individual wound-healing time and to predict the quality of wound healing.

Topical quercetin gel application improved wound healing in wistar rats

Aim: Quercetin is an antioxidant agent with proven beneficial effects in preventing inflammation. Aim of present study was to evaluate effects of quercetin gel on a secondary intention wound healing model in Wistar rats. Material and Methods: 42 Wistar rats were divided into two groups, one group administered quercetin gel (Q, n=18) while other administered vehicle (C, n=24). 5-mm diameter palatal wounds were created in each rat and all rats were given either quercetin gel or vehicle daily until sacrification days. Gel contained 5% quercetin, 5% benzocaine and glycerin, and each rat administered 0.2 ml of gel every day. Rats were sacrificed at 0th day, 7th day, 14th day, and 21st day. Fibroblast cell counts and inflammatory cell infiltration were evaluated on hematoxylin-eosin stained sections. Collagen type I and III were evaluated via immunohistochemistry. Results: Wound area decreased from day 0 to day 21 in both groups. Significant differences in wound contraction and unhealed wound area were observed between 7th and 21th days in both groups. Improvement in wound area in morphological measurements was similar in both quercetin and control groups except for 21st day on which quercetin exhibited higher wound closure. Histological findings showed significantly higher fibroblast cell counts on 7th, 14th, and 21st days in the quercetin group and difference in both quercetin. Inflammatory cell counts significantly decreased from day 0 to 21 in both groups. Collagen I synthesis were lowest on the 0th day and significantly increased on all other times in both groups. As for collagen III, the levels were highest on 7th day in both groups and significantly decreased on the 14th and 21st day. Conclusion: Present results revealed an improvement in wound healing after quercetin administration which was indicated by decreased inflammatory cells and increased fibroblast cells. However, collagen synthesis was found similar.