Abstract
Background: The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Was evaluated the effect of 627 nm wavelength LED irradiation on VEGF-α and TGF-β1 expressions in a model of secondary intention wound healing. Methods: The evaluations were performed using a dorsal puncture model and the sample consisted of 120 Wistar rats, randomly divided into LED groups submitted to 3, 7, 14 and 21 irradiation treatment for 100 s, 7 J / cm2, and control groups. Histological analysis, immunohistochemistry and contraction of the lesion were performed. Results: LED irradiation led to effective wound regeneration results. Similar effects were obtained with vascular, exudative, and proliferative phenomena. VEGF and TGF-β1 levels were increased in LED-treated groups with initial treatments. Collagen analysis revealed that LED-treated groups levels were increased on days 14- and 21 of the treatments. An acceleration in tissue regeneration was observed in LED treated groups. Macroscopically visible results showing a complete healing process was evident after 14 days of treatment. Conclusion: 627 nm LED irradiation effectively accelerated wound healing leading complete closure of the skin in a shorter time compared to control group.
Highlights
The wound healing process is a complex and dynamic mechanism that involves several factors to achieve the tissue repair, and it happens in 4 phases: hemostasis, in lammation, proliferation and remodeling Alerico et al (2015), Otterço et al (2018), Patrulea et al (2015), Singh et al (2016)
Control Group Day 21 (C21): no treatment for 21 days and euthanasia performed on the 22nd day
LIGHT EMITTING DIODE (LED) Group Day 21 (L21): treatment with 627 nm LED at the dose of 7 J/cm2 and euthanasia performed on the 22nd day
Summary
The wound healing process is a complex and dynamic mechanism that involves several factors to achieve the tissue repair, and it happens in 4 phases: hemostasis, in lammation, proliferation and remodeling Alerico et al (2015), Otterço et al (2018), Patrulea et al (2015), Singh et al (2016). A factor of great importance for the process of healing to be ef icient is TGFβ1, which is involved in almost every phase of healing, amplifying the migration of in lammatory cells, stimulating angiogenesis, formation of granulation tissue, reepithelization, and increasing the deposition of collagen III and I Campos et al (2007) Another important factor is the VEGF-α, secreted during proliferation phase, with the function of forming new blood vessels, which are necessary for synthesis, deposition and organization of the granulation tissue for the inal phases Mendonça et al (2009). Was evaluated the effect of 627 nm wavelength LED irradiation on VEGF-α and TGF-β1 expressions in a model of secondary intention wound healing
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