An 8-week growth experiment was conducted to determine the dietary protein requirement of hatchery-produced juvenile white sturgeon ( Acipenser transmontanus). Twenty-five-week-old sturgeon with an initial body weight of 145.2±0.2 g (mean±SE, n=24) were fed 1.5% of their body weight per day one of the eight purified diets which contained from 20.0 to 52.7% crude protein. Protein of these diets was supplied with graded amounts of a protein mixture (casein:wheat gluten:egg white, 62:30:8). Percent body weight increase (%BWI), feed gain ratio, protein efficiency ratio, and productive protein value were significantly ( P<0.05) affected by the dietary protein content. the protein requirement of sturgeon was estimated from the %BWI using four different statistical methods. Analysis of variance and Duncan's multiple range test suggested that the protein requirement of sturgeon was 38.4–43.0% and a broken line model fitted by least squares showed that the requirement was 40.5±1.6%. Quadratic regression analysis of the dose-response curve showed that the maximum %BWI occurred at 49.6% and the second-order polynomial regression analysis revealed that the minimum range of protein requirement was between 36.5 and 40.5%. Based on these results, 40% dietary crude protein from a good quality source is recommended for the optimum growth of hatchery-produced juvenile white sturgeon weighing between 145 and 300 g.