Sebaceous Research Articles

Piperine reduces hair oiliness by inhibiting adipogenesis of hair stem cells

Piperine, an alkaloid compound in black pepper (Piper nigrum), has beneficial bioactivities. Specifically, piperine inhibits adipogenesis in 3T3-L1 cells by suppressing the transcriptional activity of PPARγ. Control of hair oiliness, which is related to adipogenic regulation, is important to prevent hair loss. Excessive sebum from the sebaceous gland (SG) can cause acne, folliculitis, or irritated skin by clogging pores. To investigate the in vivo function of piperine in SG, we used mice fed a high-fat diet (HFD). The HFD increased the size and Oil Red O (ORO) staining intensity of SG, which were significantly reduced by piperine. The HFD also upregulated the expression of sebocyte-associated genes, including PPARγ target genes, an effect reversed by piperine. In CD34/CD49f double-positive hair follicle bulge stem cells isolated from mouse vibrissae, piperine inhibited cellular adipogenesis, likely via transcriptional repression of related genes. Furthermore, piperine reduced the thickness of subcutaneous fat. In human dermal papilla cells, piperine inhibited cellular adipogenesis, as shown by the reduction in ORO staining and the downregulation of PPARγ target genes. In conclusion, piperine can be used to reduce hair greasiness by suppressing adipogenesis in hair stem cells.

A Rare Concurrence of Polymorphic Pityriasis Rosea: Two Atypical Variants Along with a Classical Plaque Variant in the Pediatric Age Group

Pityriasis rosea, is an acute self-limiting papulosquamous inflammatory disorder. It usually starts with a herald patch on the trunk and proximal extremities, followed by scaly oval patches that give them a “Christmas tree” appearance. The lesions may be associated with fever a rash. The disease can present as numerous morphologies such as papular, urticarial, lichenoid, and generalized that may be confused with other conditions such as secondary syphilis, seborrheic dermatitis, nummular eczema, pityriasis lichenoides chronica, tinea corporis, viral exanthems, lichen planus, and PR like eruption linked to particular drugs. We report a rare case of polymorphic pityriasis rosea in paediatric age group.

Giant Cerebriform Nevus Sebaceous of Jadassohn in an Infant

Nevi are hamartomatous growth of skin or mucosa that usually present at the time of birth or soon after. Nevus sebaceous (NS) is an epidermal nevus comprised predominantly of sebaceous glands. It presents at the time of birth, as yellow-tan-colored plaque, which usually remains constant till puberty and thereafter it increases in size due to the hormonal influence on sebaceous glands. Here, we are reporting a case of giant cerebriform NS of Jadassohn in a 2-month-old male child. Such giant and cerebriform appearance of NS is rarely seen.

Vellus hair follicle diseases

Abstract Human hair follicles are composed of vellus hair follicles (vHFs), sebaceous hair follicles, and terminal hair follicles (tHFs), which share histoanatomy, but differ in biophysiology, therefore are distinctive in clinical morbidities. vHFs are much less studied, and many issues remain to be clarified, such as the expression and regulation of androgen receptors, hair follicle stem cells, immune privilege, and microbiome. Hirsutism is an example of an endocrine disorder with the pathogenesis incompletely understood, especially in idiopathic hirsutism. Interactions between androgens and other hormones deserve special attention. Treatment is unstandardized, whereas combination of antiandrogens and laser shows promise. Keratosis pilaris, a keratinization disorder, lacks clarity in etiopathogenesis, impacting esthetics and therapeutic efficacy. Among the inflammatory disorders, perioral/periorbital dermatitis arises from vHFs irritation, categorized into papular, eczematous, and granulomatous forms. This necessitates varied differential diagnoses and therapeutic approaches. Follicular eczema, a variant of atopic dermatitis in children, is also associated with xerotic dermatitis and allergic contact dermatitis in adults. Bacterial infections rarely target vHFs, in contrast to tHFs. Trichodysplasia spinulosa (TdS) links to TdS-associated polyomavirus infection localized to the inner root sheath cells of vHFs, underdiagnosed in immunocompromised patients. The varying manifestation and pathogenic significance of Malassezia furfur in Malassezia (pityrosporum) folliculitis, pityriasis versicolor, and seborrheic dermatitis remain debated. Proliferative/neoplastic entities of vHFs include vellus hair cyst, pilomatrixoma, trichoepithelioma, and trichostasis spinulosa. Follicular spiny hyperkeratosis, lichen spinulosus, and lichen scrofulosorum are reactive conditions of vHFs. Recognizing vHF-associated diseases helps us to better understand the human hair follicles and to enhance daily dermatological practices.

Enhancing assisted diagnostic accuracy in scalp psoriasis: A Multi-Network Fusion Object Detection Framework for dermoscopic pattern diagnosis.

Dermoscopy is a common method of scalp psoriasis diagnosis, and several artificial intelligence techniques have been used to assist dermoscopy in the diagnosis of nail fungus disease, the most commonly used being the convolutional neural network algorithm; however, convolutional neural networks are only the most basic algorithm, and the use of object detection algorithms to assist dermoscopy in the diagnosis of scalp psoriasis has not been reported. Establishment of a dermoscopic modality diagnostic framework for scalp psoriasis based on object detection technology and image enhancement to improve diagnostic efficiency and accuracy. We analyzed the dermoscopic patterns of scalp psoriasis diagnosed at 72nd Group army hospital of PLA from January 1, 2020 to December 31, 2021, and selected scalp seborrheic dermatitis as a control group. Based on dermoscopic images and major dermoscopic patterns of scalp psoriasis and scalp seborrheic dermatitis, we investigated a multi-network fusion object detection framework based on the object detection technique Faster R-CNN and the image enhancement technique contrast limited adaptive histogram equalization (CLAHE), for assisting in the diagnosis of scalp psoriasis and scalp seborrheic dermatitis, as well as to differentiate the major dermoscopic patterns of the two diseases. The diagnostic performance of the multi-network fusion object detection framework was compared with that between dermatologists. A total of 1876 dermoscopic images were collected, including 1218 for scalp psoriasis versus 658 for scalp seborrheic dermatitis. Based on these images, training and testing are performed using a multi-network fusion object detection framework. The results showed that the test accuracy, specificity, sensitivity, and Youden index for the diagnosis of scalp psoriasis was: 91.0%, 89.5%, 91.0%, and 0.805, and for the main dermoscopic patterns of scalp psoriasis and scalp seborrheic dermatitis, the diagnostic results were: 89.9%, 97.7%, 89.9%, and 0.876. Comparing the diagnostic results with those of five dermatologists, the fusion framework performs better than the dermatologists' diagnoses. Studies have shown some differences in dermoscopic patterns between scalp psoriasis and scalp seborrheic dermatitis. The proposed multi-network fusion object detection framework has higher diagnostic performance for scalp psoriasis than for dermatologists.

Extraocular Sebaceous Carcinoma in the Lower Back: Rare Presentation of a Great Masquerader

Abstract Malignant adnexal tumors of the skin are rare. Sebaceous carcinoma (SC) is an aggressive malignant tumor of the sebaceous glands. It is traditionally classified into periocular and extraocular types. We present a rare case of extraocular SC of a 38-year-old female who presented with nontender swelling over the right flank for 1 year. Clinically, it was diagnosed with a sebaceous cyst. The tumor was excised and sent for histopathological examination. Sections showed sheets and lobules of tumor cells separated by fibrovascular stroma. Individual tumor cells were atypical basaloid to clear cells with prominent pleomorphism. Mitosis was brisk with areas of necrosis. On immunohistochemistry, the tumor cells were focally positive for epithelial membrane antigen and negative for S100 and PAX8 with a high Ki-67 index of 80%. Her postoperative period and follow-up for 1 year have been uneventful. This case is being reported for its rarity.

Association of Fordyce Granules with Skin Types – A Cross-Sectional Study

ABSTRACT Background: Fordyce granules, sometimes referred to as Fordyce dots, are aberrant sebaceous glands that present as diminutive, non-painful, elevated lesions exhibiting a yellowish or whitish hue, measuring 1 to 3 mm in diameter, and manifesting inside the oral cavity. Likewise, these particles may also be seen in the vaginal region and inside the oral cavity. Aim: The primary objective of the current study is to evaluate the potential association between Fordyce granules and the skin type of individuals who seek dental care at the Dental Clinics of Qassim University. Materials and Methods: The current cross-sectional research was undertaken at the Dental Clinics of Qassim University, with a sample of 87 patients diagnosed with Fordyce’s granules. The research consisted of a heterogeneous sample of participants, including individuals of all genders, ranging in age from 18 to 85 years. The study included a comprehensive evaluation of several anatomical regions to identify the existence of Fordyce’s granules. This examination was conducted by a single examiner who had undergone calibration. Additionally, the participants’ skin types were established using the Baumann Skin Typing System questionnaire. The data that was gathered was afterward analyzed utilizing statistical methods via the use of SPSS software. A pre-set significance level was established at P < 0.05. Results: The distribution of skin types among the study participants with Fordyce’s granules were found to be oily skin (51.3%), dry skin (47.9%), sensitive skin (49.3%), and resistant skin (56.3%). The results of the research showed that there was no statistically significant correlation between the two variables, i.e. skin type and the presence of Fordyce’s granules. This conclusion is supported by the increased P values of 0.941 for those with oily skin and 0.785 for individuals with dry skin. Conclusion: No relation between skin type and Fordyce’s granules in the current study.

Dysmenorrhea and Seborrheic Dermatitis due to Occupational Stress Among Female Bank Workers

Introduction: Bank workers who provide services to customers are required to have an optimal performance. They are vulnerable to stress that can interfere with their performance. Stress causes an increase in sebaceous glands, resulting in seborrheic dermatitis and an increase in hormones. This can cause contraction of the uterine muscles which can lead to dysmenorrhea. This study aims to determine the relationship between occupational stress and dysmenorrhea and seborrheic dermatitis among female bank workers. Methods: This research followed an analytic observational cross-sectional design using primary data from female workers at SumSel Babel Bank A. Rivai Branch. The sample consisted of 75 female bank workers, who met the inclusion and exclusion criteria and were selected using consecutive sampling. The independent variable in this study was occupational stress, while the dependent variables were dysmenorrhea and seborrheic dermatitis. Data were collected from primary data, the workplace stress scale questionnaire for occupational stress, the WaLIDD score for dysmenorrhea, and seborrheic dermatitis examination by a dermatologist. After obtaining the data, bivariate analysis was performed using the Chi-square test. Results: Sixty respondents (80%) had occupational stress, 47 respondents (62.7%) experienced dysmenorrhea, and 69 respondents (92%) did not experience seborrheic dermatitis. The results of statistical tests showed that there was a relationship between occupational stress and dysmenorrhea in female bank workers with a p-value 0.000 and no significant relationship between stress and the incidence of seborrheic dermatitis with p-value 0.202. Conclusion: Occupational stress can cause health problems including dysmenorrhea in female workers.

Education on acne and cosmetic knowledge for adolescents at SMK Rise Kedawung

Acne occurs due to the presence of S. Aureus bacteria, when the oil glands in the skin are too active, acne usually appears on the surface of the skin of the face, neck, chest, and back. Therefore, antibacterials are needed to overcome this, which can be obtained from chemicals or traditional plants. The aim of this community service program is to provide education to students at Rise Kedawung Vocational School about acne on teenage skin and training to check cosmetics registration using the BPOM Mobile application. This activity is carried out by providing material about acne starting from understanding acne, the causes of acne, traditional plants that have antibacterial activity, chemicals that have antibacterial activity, and how to check BPOM registration. The pre-test and post-test results showed an increase in participants' understanding regarding acne, acne medication, and checking cosmetic registration from 87% to 96%.

Risk assessment of pressure injuries in newborns. Appropriateness of Glamorgan and NSRAS scales: a scoping review

The newborn's skin must undergo a transition process as a result of the passage from an aqueous to an aerobic environment. This process occurs over a period of approximately 2 to 8 weeks. The skin of newborns has important anatomical and physiological differences compared to those of older children and adults. It is thin, with fewer appendages; the stratum corneum is not present; the dermal-epidermal junction is reduced; intercellular junctions are weaker; the secretions of the sebaceous glands are limited and the pH is generally neutral. All these factors make the newborn's skin more fragile to any stimulus. To have effective prevention and intervention procedures, an accurate and practical risk assessment tool should be identified as a preliminary step for adequate prevention. Unfortunately, only few validated tools are available to assess the risk of PUs in children, especially newborns. The objective of the study is to examine the adherence of the most used tools for the assessment of PU risk in newborns, in particular to make a comparison of the limits and advantages of the most frequently used tools in neonatal settings, the Glamorgan scale and NSRAS. To respond to the main objective, a scoping review of the literature was conducted. 54 studies were identified. Further analysis was conducted, which led to the exclusion of articles that did not examine the characteristics of newborn skin, the risk factors related to PUs and the appropriateness of the NSRAS and Glamorgan tools, for a final selection of 35 studies. Patients admitted to NICU represent the paediatric category most exposed to the risk of developing pressure ulcers. Factors such as reduced mobility, together with the pressure exerted by aids or devices, increase the risk of pressure injuries. The risk factors that most expose the newborn to risk are the structure of the skin and medical devices. Nurses should implement preventative measures to control the risk of PU. The use of specific tools is necessary to detect the risk of PUs in newborns and implement preventive measures. PUs risk assessment is one of the nursing care strategies for prevention. Therefore, operators need a valid, reliable, and predictive scale. Lastly, we recommend the use of NSRAS for future research and for the education of healthcare professionals in the neonatal area.

Zinc, Copper, and Iron in Selected Skin Diseases.

Trace elements are essential for maintaining the body's homeostasis, and their special role has been demonstrated in skin physiology. Among the most important trace elements are zinc, copper, and iron. A deficiency or excess of trace elements can be associated with an increased risk of skin diseases, so increasing their supplementation or limiting intake can be helpful in dermatological treatment. In addition, determinations of their levels in various types of biological material can be useful as additional tests in dermatological treatment. This paper describes the role of these elements in skin physiology and summarizes data on zinc, copper, and iron in the course of selected, following skin diseases: psoriasis, pemphigus vulgaris, atopic dermatitis, acne vulgaris and seborrheic dermatitis. In addition, this work identifies the potential of trace elements as auxiliary tests in dermatology. According to preliminary studies, abnormal levels of zinc, copper, and iron are observed in many skin diseases and their determinations in serum or hair can be used as auxiliary and prognostic tests in the course of various dermatoses. However, since data for some conditions are conflicting, clearly defining the potential of trace elements as auxiliary tests or elements requiring restriction/supplement requires further research.

Safety, Efficacy and Attributes of 2.5% Selenium Sulfide Shampoo in the Treatment of Dandruff: A Single-Center Study.

Selenium sulfide, available as a shampoo or topical lotion at 1%, or 2.5% concentrations in India, is used as a topical antiseborrheic and antifungal for the treatment of dandruff, seborrheic dermatitis, psoriasis, and tinea versicolor. In the present study, the safety, efficacy, and attributes of 2.5% selenium sulfide shampoo were evaluated in Indian participants with dandruff. A single-center, single-arm, prospective, investigator-initiated, open-label, post-marketing interventional study was conducted on Indian subjects aged 18-70 years diagnosed with moderate dandruffwho were prescribed 2.5% selenium sulfide shampoo every three days for four weeks. The primary endpoints were 1) reduction in total dandruff score assessed using a clinical grading scale for adherent and loose dandruff from baselineto weeks 1, 2, and 4, and 2) incidence of adverse events up to the end of the study. The key secondary endpoints were 1) participants' perception of shampoo attributes (dandruff reduction, scalp itch, scalp oiliness/greasiness, or fragrance) as assessed by a subjective self-assessment questionnaire post-first wash and at weeks 1, 2, and/or 4; 2) satisfaction with treatment as assessed by investigators and participants using a subjective self-assessment questionnaire at week 4; and 3) reduction in scalp sebum as assessed with a meibometer at weeks 2 and 4.Statistical analysis was performed using the Wilcoxon signed-rank test for continuous variables and the Chi-square test for categorical variables. A p-value of 0.05 was considered to be statistically significant. Of 34 enrolled subjects, 30 completed the four-week study. The mean (standard deviation, SD) age of the study participants was 29.8 (7.87) years, with the majority being females (n=18; 60.0%). Mean (SD) total dandruff score significantly (p=0.001) reduced from a baseline score of 11.5 (2.15) to 7.17 (2.12) at week 1, 4.93 (1.72) at week 2, and 2.5 (1.17) at week 4. All the participants reported dandruff reduction and acceptable fragrance of the shampoo at four weeks. Absence of itching and reduction in oiliness was reported by 73.3% (n=22) of participants at week 4 and by 50.0% (n=15) of participants at week 2, respectively. All participants reported good, very good, or excellent satisfaction with the test shampoo at week 4, whereas the investigators rated the shampoo as very good or excellent in managing dandruff in all participants. At week 4, erythema was reported to be absent in all participants. No adverse events were reported during the study. The 2.5% selenium sulfide shampoo was found to be effective in the management of dandruff and related symptoms like itching, oiliness, and greasiness and had a good safety profile in Indian participants with dandruff.

Significant Efficacy of Traditional Chinese Medicine and Herbal Treatment on Chronic Seborrheic Dermatitis: A Case Report

This case report presents a 27-year-old female who has been suffering from Seborrheic Dermatitis (SD) since the age of 12. After years of ineffective treatment with Western medicine, she turned to traditional Chinese medicine and achieved significant improvement.

Formulation and Physical Evaluation of Papaya Leaf (Carica papaya L.) Ethyl Acetate Fraction Cream Preparation as Anti Acne Against Propionibacterium acne Bacteria

Acne or acne vulgaris is a disease in the form of inflammation of multiple sebaceous glands caused by the bacteria Propionibacterium acne. Papaya leaves are a plant that can potentially be used as an acne treatment. To determine the antiacne activity of papaya leaf ethyl acetate fraction cream preparations caused by Propionibacterium acne bacteria. The ethyl acetate fraction of papaya leaves are made into a cream preparation with varying concentrations of ten, fifteen, and twenty percent. The results of the cream preparation test met the physical property standards. Still, the viscosity in formulation three did not meet the standards, and formulas two and three did not meet the cream stability test standards. Antibacterial tests show that all formulas have inhibitory power against bacteria. The average inhibition zone results in formula one, formula two, and formula three were 4.99 ± 0.48 mm, 6.85 ± 0.48 mm and 10.12 ± 1.42 mm. Formula One papaya leaf fraction cream fulfills all the physical characteristics of the preparation. All papaya leaf ethyl acetate fraction cream preparations are antibacterial against Propionibacterium acne. So, research can be developed using the ethyl acetate fraction of papaya leaves in various preparations, and activity tests against other bacteria can be carried out.

The Evaluation of Dermatological Disease Profiles of Neurosurgery Inpatients: A Tertiary Clinic Experience.

Both the skin and neuronal systems originate from the ectoderm. In patients hospitalized for neurosurgery, their skin may be affected by genetic and environmental factors. This study researched disease relationships by evaluating the profile of hospitalized neurosurgery patients who consulted with dermatologyin a tertiary clinic (Neurosurgery Clinic, Ankara Training and Research Hospital, Ankara). This study included hospitalized neurosurgery patients who consulted with dermatology. Age, gender, type of hospitalization, neurosurgical diseases, and dermatology diseases were selected as study variables. The medical health records of the patients were retrospectively scanned and analyzed. A total of 172 consultations were analyzed. The mean age of patients was 44.7 years oldranging from 1 year to99 years old. The percentage of male patients was 54.7%; 25.5% of the patients were hospitalized for cerebral vascular diseases, 30.2% for spinal diseases, 22.1% for tumors, 12.2% for infections, and 1.2% for other neurosurgical diseases. The most commonly diagnosed dermatological disease in patients was drug eruptions (18.6%), followed by seborrheic dermatitis (16.2%) and contact dermatitis (14.5%). According to this study, the most commonly diagnosed dermatological diseases in neurosurgery inpatients were drug eruption, contact dermatitis, and seborrheic dermatitis. The results of this study may be helpful in terms of neurosurgical training planning and treatment management.

Ovarian dermoid cyst in a buffalo

AbstractOvarian dermoid cyst is characterised by the presence of hair follicles, sebaceous glands grossly observed as clusters of matted hair in greyish cheesy material in the vicinity of ovaries. Upon examination of 235 buffalo genitalia, five were found to have abnormal ovarian texture and were relatively large in size. Further investigation revealed presence of dermoid cyst in these ovaries. Histopathological analysis showed degenerated primary, secondary and tertiary/Graafian follicles in the ovarian parenchyma, with patches of neuro‐ectodermal rosette‐like structures composed of glial cells. Other extra‐gonadal tissues often less organised were also observed like cartilage, fibrous connective tissue and glandular structures. In two of the specimens, corpus luteum with degenerated luteal cells was also present in the parenchyma of the ovary. These detailed investigations pointed to the infertility problems in buffaloes associated with this condition and often dictated in slaughterhouse‐derived ovaries. It has clinical significance as these structures are often confused with cystic ovarian disease upon rectal palpation, and treatment is unsuccessful in reducing the size of the ovary at the field level.

Neoadjuvant Chemotherapy in Non-Metastatic Eyelid Sebaceous Gland Carcinoma: A Report in 10 Cases

Introduction: Eyelid sebaceous gland carcinoma (SGC) is an aggressive malignancy. Surgical excision is the standard treatment for non-metastatic eyelid SGC. This study aimed to evaluate treatment outcomes with use of neoadjuvant chemotherapy (NACT) and any change in ease/difficulty of surgical treatment in such cases. Methods: This was a prospective interventional study conducted over 24-month period. Histopathologically, confirmed cases without any systemic metastasis were included. Clinico-demographic details were collected for 30 patients. 10 patients received NACT using cisplatin and 5-FU. Tumour response was evaluated using RECIST criteria. An ease of surgery questionnaire was used to assess difficulty of surgical treatment before and after NACT. Results: Of the 30 patients evaluated for recruitment, 37% had recurrent SGC and 72% had advanced tumour stage. Ten patients were recruited for NACT. There was partial response in 80% and complete response in 10% cases. Tumour T category downstaging was seen in 50% of cases. While tumour dimensions/volume reduced substantially, surgical ease improved in only 40% cases. Conclusion: A significant proportion of SGC patients evaluated in our study presented with recurrent and/or advanced disease. NACT caused tumour regression in 90% of cases. However, surgical ease improvement was limited, pointing to a need for surgical modification in such cases. Corneal ulceration was noted in 2 cases with large tumours causing a complete mechanical ptosis. Overall, the study introduced an ease of surgery questionnaire and provided insights into benefits and challenges of using NACT for eyelid SGC management.

Acupuncture Treatment of Autonomic Nervous Dysfunction in Patients with Parkinson's Disease: A Narrative Review.

Parkinson's disease (PD) is a neurodegenerative disease affecting middle-age and elderly people. Its clinical manifestations include a series of progressive motor and nonmotor symptoms (NMS). Autonomic nervous dysfunction is a kind of NMS that includes constipation, hyperhidrosis, seborrheic dermatitis (sebaceous surface), salivation, sexual dysfunction, voiding dysfunction, and orthostatic hypotension. The incidence of autonomic nervous dysfunction is high, and the clinical manifestations are complex and changeable. It can occur before motor symptoms emerge, is closely related to the prognosis of PD, and affects quality of life seriously. Routine anti-PD drugs have little effect on improving autonomic nervous dysfunctions. Acupuncture for autonomic nervous dysfunction in PD induces a certain effect without adverse reactions, with potential advantages and broad prospects for application. This article summarizes and discusses the pathogenesis of autonomic nerve dysfunction in PD and acupuncture therapy for the condition to serve as a reference for clinical research.

Exploring the antibiofilm efficacy of cinnamaldehyde against Malassezia globosa associated pityriasis versicolor

BackgroundMalassezia globosa is a commensal basidiomycetous yeast occurring on the skin that causes pityriasis versicolor (PV) and seborrheic dermatitis, but that has also been implicated in other dermatoses. Cinnamaldehyde (CM) has antibacterial, antioxidant, and anti-inflammatory activities, but the effect of CM on M. globosa-infected PV has not been clarified. PurposeThe study aimed to investigate the possible antifungal and antibiofilm activities of CM against M. globosa-infected PV in vivo and in vitro. MethodsThe broth microdilution method was used to determine the minimum inhibitory concentration (MIC) of CM against M. globosa. The crystal violet staining assay and XTT assay were used to investigate the inhibition of CM on biofilm formation and the eradication of mature biofilms. The visualizations of the biofilm and cell distribution in the biofilm matrix were performed with a scanning electron microscope and confocal laser scanning microscope. The kits of antioxidant kinase were used to determine the activities of oxidative stress markers in M. globosa-stimulated HaCaT cells. Western blot assays were used to evaluate the role of TLR2/NF-κB in vitro. Furthermore, the protective effect of CM was assessed in M. globosa-associated PV mice. The expressions of inflammatory cytokines and apoptosis were screened using ELISA assays. The expressions of interleukin-6 and tumor necrosis factor-α were measured by an immunohistochemistry method in vivo. ResultsOur results showed that the MIC of CM against planktonic cells of M. globosa was 4 µg/ml and treatment with 20 × MIC CM eradicated mature biofilms of M. globosa. In vitro, after CM treatment the levels of oxidative stress indicators (i.e., superoxide dismutase, catalase, glutathione) significantly increased, while the levels of malondialdehyde decreased. In addition, the expression of TLR2/NF-κB in HaCaT cells was significantly reduced after CM treatment. On the other hand, an in vivo therapeutic effect of CM was assessed against M. globosa-infected mice. The fungal load on the skin decreased after treatment with CM compared to the M. globosa-infected group. In addition, the uninfected animals showed a normal skin structure, whereas, the M. globosa-infected mice showed extensive infiltration of neutrophils in skin tissues that improved after treatment with CM. Meanwhile, the levels of inflammatory and apoptotic factors improved after CM treatment. ConclusionOur results showed that CM inhibits the biofilm formation of M. globosa and eradicates mature biofilms of M. globosa. Treatment with CM significantly decreased oxidative stress, apoptosis, and inflammatory markers in the skin tissue and HaCaT cells. Hence, this study suggests that CM is a good candidate therapeutic agent against M. globosa-induced PV infections because of its antifungal, antibiofilm, and anti-inflammatory properties.

Impact of skin sensitivity mechanisms on sebum secretion: Management strategies for oily sensitive skin

Recent epidemiological surveys based on Baumann's classification have revealed a notable rise in the prevalence of oily sensitive skin. The development of oily sensitive skin is primarily linked to the abnormal function of sebaceous glands. The function of sebaceous glands is regulated by several factors, including inflammatory mediators, neurotransmitters, and endocrine signals. Sensitive skin, particularly oily sensitive skin, is prone to local inflammation, which in turn disrupts the normal functioning of sebaceous glands. This creates a loop wherein increased oil production exacerbates sensitivity, while heightened sensitivity further stimulates sebum secretion, perpetuating a vicious cycle. This article summarizes our understanding of the four primary mechanisms underlying skin sensitivity and their impact on sebaceous gland activity. Accordingly, it proposes management strategies for oily sensitive skin and seeks to guide the development of skin care regimens for this skin type.