Event Abstract Back to Event Optimization of extraction conditions of phenolic bioactive compounds from Fucus spiralis and Bifurcaria bifurcate using response surface methodology Daniela Teodoro1, Andre Horta1 and Maria M. Gil1* 1 Centro de Ciências do Mar e do Ambiente (IPLeiria), Portugal Seaweeds (SW) consumption has increased worldwide due to the growing awareness of its several benefits. In fact, edible SW contains valuable biomolecules, namely essential amino acids, essential elements, vitamins, important lipids, carbohydrates and dietary fiber. Additionally, they are an excellent source of phenolic compounds considered as one of the most important classes of natural antioxidants. Besides having antioxidant properties, phlorotannins (a group of phenolic compounds) have also been described as having: protection against vascular diseases, anticancer and antihypertension activities, anti-inflammatory properties, and antimicrobial activity against food-borne bacteria. Thus, attracting great interest from researchers and food industries. In this context, optimization of extraction conditions of phenolic bioactive compounds using “green” extraction solvents or “green” methods is of great importance to the industry. The extraction of phenolic compounds from Fucus spiralis and Bifurcaria bifurcate was optimized using a response surface methodology (RSM). A Box-Behnken design was conducted to analyse the effects of solvent concentration (ethanol to water ratio), time and solid–liquid ratio, e.g., biomass concentration (g dry seaweed per 100 ml solvent) on the extraction of total phenolic content. Quadratic polynomial regression models were fitted through the experimental results. The optimum extraction conditions were found to be 2.75 h extraction time, a solid–liquid ratio of 10 g dry biomass/100 mL or 30 g dry biomass/100 mL and a water-ethanol ration of 1:1 or 100%ethanol, for Bifurcaria bifurcate and Fucus spiralis, respectively. Finally, a comparison among traditional solid-liquid extraction and advanced techniques such as microwave-assisted extraction was performed to test their efficiency towards the extraction of phenolic compounds from seaweeds. Extractions were carried out at the previously best selected conditions for each technique. It is concluded that microwave is a technology that accelerate the extraction process. Acknowledgements This work had the support of Fundação para a Ciência e Tecnologia (FCT), through the strategic project UID/MAR/04292/2013 granted to MARE. Keywords: Fucus spiralis, Bifurcaria bifurcate, Response Surface Methodology, Polyphenols, Microwave Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018. Presentation Type: Oral Presentation Topic: Seafood Technology Citation: Teodoro D, Horta A and Gil MM (2019). Optimization of extraction conditions of phenolic bioactive compounds from Fucus spiralis and Bifurcaria bifurcate using response surface methodology. Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00153 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 May 2018; Published Online: 07 Jan 2019. * Correspondence: PhD. Maria M Gil, Centro de Ciências do Mar e do Ambiente (IPLeiria), Peniche, Portugal, maria.m.gil@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Daniela Teodoro Andre Horta Maria M Gil Google Daniela Teodoro Andre Horta Maria M Gil Google Scholar Daniela Teodoro Andre Horta Maria M Gil PubMed Daniela Teodoro Andre Horta Maria M Gil Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.