Sealed Ends Research Articles

Theoretical and experimental investigation of flexible air spring stiffness in a tuned liquid column gas damper for vertical vibration control

This study investigates the effectiveness of equivalent linear air spring stiffness in a Vertical Tuned Liquid Column Gas Damper (VTLCGD) for reducing vertical structural vibrations, particularly considering different sealing conditions. The VTLCGD system, designed with a single sealed vertical air column, allows flexible frequency tuning by adjusting the air spring stiffness. It aims to be used in low-frequency structures where conventional VTLCGD designs with two sealed ends are less efficient. The geometric configuration and working principle of the VTLCGD are first described. The equation of motion is derived using liquid dynamic equilibrium and the pressure-volume relationship, with expressions for natural frequency and control force validated through shaking table tests conducted with varying VTLCGD lengths. Experimental investigations are conducted to examine the parameters affecting damper performance using pressure data. The system's vibration reduction performance is then numerically evaluated on a cantilevered floor subjected to human walking. The numerical results, based on the equation of motion for liquid displacement and natural frequency, show good agreement with experimental data, which confirms the effectiveness of using linearized air spring stiffness for frequency tuning. The effects of total liquid length and height difference on control force are further investigated, and a polytropic index of 1.2 is determined for the VTLCGD frequency formula. The VTLCGD system achieves a maximum vibration reduction of 52.63 % in acceleration response on the cantilevered floor compared to the uncontrolled case. Moreover, the variation in stiffness due to changes in the sealing condition is presented to validate the damper's adaptability over a wide frequency range.

Exploring the Cutting Process of Coaxial Phase Change Fibers under Optical Characterization Tests

Urban heat islands (UHI) are a growing issue due to urbanization, causing citizens to suffer from the inadequate thermal properties of building materials. Therefore, the need for climate-resistant infrastructure is crucial for quality of life. Phase change materials (PCMs) offer a solution by being incorporated into construction materials for thermoregulation. PCMs store and release heat as latent heat, adjusting temperatures through phase changes. Polymeric phase change fibers (PCFs) are an innovative technology for encapsulating PCMs and preventing leaks. This study produced PCFs via wet-spinning, using commercial cellulose acetate (CA, Mn 50,000) as the sheath and polyethylene glycol (PEG 2000) as the core. The PCFs were cut using a hot-cutting method at three different temperatures and washed with distilled water. Morphological analysis was conducted with a bright-field microscope, and chemical analysis was performed using Fourier transform infrared spectroscopy (FTIR) before and after controlled washing. Additionally, the washing baths were analyzed by UV-visible spectroscopy to detect PEG. The PCFs displayed a well-defined core-shell structure. Although some PEG 2000 leakage occurred in unsuccessful cuts, cuts at 50 °C showed sealed ends and less material in the baths, making it viable for civil engineering materials.

Design of Gusseted Pouch Motors for Improved Soft Pneumatic Actuation

The performance of soft robots depends in large part on the actuation performance of the soft actuators used to drive them, and those making use of fluidic actuation have gained the most traction in the form of pneumatic artificial muscles (PAMs). These PAMs make use of the change in volume of a structure through pressurization of their volume to produce mechanical work. However, their performance depends in large part on how this volume deforms throughout the motion. Pouch motors have been proposed as a lightweight yet powerful soft actuator, but their sealed ends limit their performance. This article proposed the addition of gussets onto pouch motors to form gusseted pouch motors for improved performance. This allows for the actuator to produce a larger contraction ratio than without gussets and allows for the force to increase as the length of the actuator increases. This article explains the manufacturing of the actuator, proposes a numerical model that predicts well the performance of the actuator, shows experimental results for different actuator dimensions, and provides a comparison with regular pouch motors. It then demonstrates stiffness control using antagonistic pouch motors and implements the actuator into a simple robotic arm with angular position control.

Analysis of dynamic characteristics of a sealed ends squeeze film damper considering the fluid inertia force

1. Booker J., 1965, A table of the journal-bearing integral, Journal of Basic Engineering, 87, 2, 533-535. CrossRef Google Scholar

A Model and Experimental Validation for a Piston Rings—Squeeze Film Damper: A Step Toward Quantifying Air Ingestion

Abstract Rotor-bearing systems often rely on squeeze film dampers (SFDs) to increase the dynamic stability and reduce rotor motion amplitudes while traversing critical speeds. In aircraft engines, to increase the damping capacity within a limited physical space, piston rings (PRs) seal the axial ends of a squeeze film land. However, lubricant still leaks through the slit in the PR abutted ends, and air enters into the film to make a bubbly oil mixture with a much reduced damping capability. This paper presents a volume of fluid (VOF) model for a PR sealed ends SFD facing ambient air (not submerged in lubricant and prone to air entrainment) and delivers predictions benchmarked against experimental results obtained in a dedicated test rig. A lubricant feeds pressure and flows large enough to prevent air ingestion vary with the damper geometry, the lubricant inlet and outlet conditions, and the kinematics of the journal. A parametric study for a typical SFD shows the period-averaged gas volume fraction (GVF) increases as the journal squeeze film velocity (vs) increases and as the film clearance decreases. Most importantly, the location of the PR slit relative to the feedhole affects the amount of air drawn into the film. When the PR slit faces a feedhole, the film land is mostly filled with a pure lubricant. The GVF increases as the arc distance from the PR slit to the feedhole increases. The physical model, anchored by the test data, effectively addresses a fundamental challenge in SFD design and operation; namely, quantifying the amount of air ingested and its effect on the damper forced performance.

Failure behavior of the ceramic thin-walled cylindrical shell under the hydrostatic pressure

Due to the ceramic with high compressive strength and superior corrosion resistance, using ceramic as the parent material of underwater pressure shell is a reliable choice. The failure behaviors and ultimate strength of the 99% alumina ceramic cylindrical shells with sealed ends under the hydrostatic pressure are systematically investigated by means of theoretical, experimental and numerical methods. The theoretical prediction of two possible failure modes, including both the circumferential buckling and strength failure, of the cylindrical shell is firstly made. Then, the hydrostatic tests are carried out for the ceramic cylindrical shell specimens with a constant thickness-radius ratio (t/R = 0.07) and different length-radius ratios (L/R = 3, 4, 5, 6), and the ultimate pressures of the corresponding specimens are acquired. Good agreement among the theoretical, numerical and experimental results has been obtained. Moreover, the results show that the structural tolerance pressure can be effectively improved by increasing the thick-radius ratio or decreasing the length-radius ratio. Meanwhile, the conversion of two possible failure modes of the 99% alumina ceramic has been clearly revealed through changing the geometrical parameters of the cylindrical shell. This work provides the engineering guidance for the underwater pressure-resistant structural design.

Pinching of gel-filled honeycomb

The effect of gel-filling of a hexagonal honeycomb upon its transverse compressive response is investigated experimentally and numerically. The specimens comprise square tubes with sealed ends and are made from aluminium alloy honeycomb. The specimens are loaded transversely between frictionless flat platens at their mid-length. It is shown experimentally and by finite element simulation that gel-filling of the hexagonal honeycomb in the closed-ended tubes changes the deformation mode in the pinched zone from that of the empty honeycomb. The pinch load increases with increasing displacement, with no evidence of crush band formation. The highly stable response is due to the presence of the incompressible gel-core, and due to the build-up of membrane tension, in the axial direction, within the walls of the honeycomb. As pinching proceeds, axial flow of the gel occurs from the shrinking, pinched zone to the outer, free-standing, dilating portions of the tube. Additional finite element simulations quantify the sensitivity of pinch strength to the inclination of the cell walls of the honeycomb, and to the presence of geometric imperfections within the honeycomb.

On the Experimental Dynamic Force Performance of a Squeeze Film Damper Supplied Through a Check Valve and Sealed With O-Rings

Abstract In rotor-bearing systems, squeeze film dampers (SFDs) assist to reduce vibration amplitudes while traversing a critical speed and also offer a means to suppress rotor instabilities. Along with an elastic support element, SFDs are effective means to isolate a rotor from its casing. O-rings (ORs), piston rings (PRs), and side plates as end seals reduce leakage and air ingestion while amplifying the viscous damping in configurations with limited physical space. ORs also add a centering stiffness and damping to a SFD. The paper presents experiments to quantify the dynamic forced response of an O-rings sealed ends SFD (OR-SFD) lubricated with ISO VG2 oil supplied at a low pressure (0.7 bar(g)). The damper is 127 mm in diameter (D), short in axial length L = 0.2D, and the film clearance c = 0.279 mm. The lubricant flows into the film land through a mechanical check valve and exits through a single port. Upstream of the check valve, a large plenum filled with oil serves to attenuate dynamic pressure disturbances. Multiple sets of single-frequency dynamic loads, 10 Hz to 120 Hz, produce circular centered orbits with amplitudes r =0.1c, 0.15c, and 0.2c. The experimental results identify the test rig structure, ORs, and SFD force coefficients, namely, stiffness (K), mass (M), and viscous damping (C). The ORs' coefficients are frequency independent and show a sizeable direct stiffness, KOR ∼ 50% of the test rig structure stiffness, along with a quadrature stiffness, K0 ∼ 0.26KOR, demonstrative of material damping. The lubricated system damping coefficient equals CL = (CSFD + COR); the ORs contributing 10% to the total. The experimental SFD damping and inertia coefficients are large in physical magnitude; CSFD slightly grows with orbit size, whereas MSFD is relatively constant. The added mass (MSFD) is approximately fourfold the bearing cartridge (BC) mass; hence, the test rig natural frequency drops by ∼50% once lubricated. A computational physics model predicts force coefficients that are just 10% lower than those estimated from experiments. The amplitude of measured dynamic pressures upstream of the plenum increases with excitation frequency. Unsuspectedly, during dynamic load operation, the check valve did allow for lubricant backflow into the plenum. Post-tests verification demonstrates that, under static pressure conditions, the check valve does work since it allows fluid flow in just one direction.

Periodic Vibrations of a Beam with Rigidly Sealed Ends

We study the problem of searching periodic solutions to the Euler–Bernoulli equation governing vibrations of a beam with the boundary conditions corresponding to the case of rigidly sealed beam ends. The nonlinear term satisfies the nonresonance condition at infinity. We establish the existence and uniqueness of a solution. To prove the results, we use topological methods (the Leray–Schauder principle) as well as variational methods (the mountain pass theorem).

On the Effect of the Gap of End Seals on Force Coefficients of a Test Integral Squeeze Film Damper: Experiments and Predictions

Abstract An integral squeeze film damper (ISFD) offers the advantages of a lower number of parts, a shorter axial span, a lighter weight, a split manufacturing, and high precision on its film clearance construction. An ISFD does not only add damping to reduce shaft vibration amplitudes and to enhance the stability of a rotor-bearing system but also can be used to tune a rotor-bearing system natural frequency, and thus increasing the operational safety margin between the running shaft speed and the system critical speed. In spite of the numerous commercial applications, the archival literature is scant as per the experimental quantification of force coefficients for ISFDs. This paper details the results of an experimental and analytical endeavor to quantify and to predict the dynamic force coefficients of an ISFD, hence bridging the gap between theory and practice. With an axial length of 76 mm, the test damper element has four arcuate film lands, 73 deg in arc extent at a diameter of 157 mm, and each with a clearance (c) equaling to 0.353 mm. As is customary, the damper has its axial ends sealed with end plates produced by a set of installed shims giving an axial gap (d) equal to 1.5c, 1.21c, and 0.8c. A baseline configuration, namely, open ends, is also tested without the end seals in place. In the test rig, the ISFD and its housing are flexibly mounted while the rotor is rigid and stationary (no spinning). The lubricant is an ISO VG46 oil supplied at a low pressure, 1 to 2 bar(g) and ∼47 °C inlet temperature, typical of compressor applications. The test procedure applies static loads on the ISFD and records the bearing static offset or eccentricity to verify the structure stiffness, and meanwhile, individual hydraulic shakers deliver dynamic loads along two orthogonal directions to produce motions over a set frequency range, 10 Hz to 160 Hz. The ISFD produces direct damping and inertia that increase with the journal static eccentricity albeit at a lower rate than predictions from a computational squeeze film flow model that includes lubricant compressibility. The end seals are effective in significantly raising the damping coefficient while reducing the oil through flow rate. The damper with the tightest sealed ends (d = 0.8c) shows nearly 20 times more damping that the open ends ISFD albeit also revealing a significant stiffness hardening (negative virtual mass) as the excitation frequency increases. On the contrary, the open ends ISFD and the sealed-ends configurations with gaps d = 1.21c and 1.5c produce a (positive) virtual mass that exceeds the test element physical mass and thus softens the test element direct dynamic stiffness. For the configurations with loose end seals (d = 1.21c or larger to open ends), the model predicts well the damping coefficients but under predicts the added masses by 50% or more. Note this virtual mass coefficient, largely ignored in practice, can make the test element either extremely stiff as with the sealed damper configuration with the smallest gap d = 0.8c, or very soft as with the ISFD with end seals gap = 1.21c or 1.5c. Hence, designers are cautioned not to pursue overly tight end sealed dampers as the mineral lubricant, nearly incompressible though always having a small amount of entrapped gas, may behave distinctly when confined to a squeezed film volume and having no adequate routes to escape or flow through.

Reverse pulsed-flow cleaning of pleated filter cartridges having an inner pleated filter cone

Different from conventional pleated filter cartridges, which apply pleated filter media as the cartridge sidewall, seal one end as the base and open the other end as the entrance for cleaning flow, ones with inner pleated filter cones, called as cone pleated filter cartridges, have been recently proposed to increase the filtration area and to improve the efficiency of the reverse pulsed-flow cleaning. This work investigated the cleaning performance of cone pleated filter cartridges via the numerical modeling. Our modeling shows that, for a cone pleated filter cartridge cleaned by a reverse pulsed-flow, a high- and low- pressure zones were developed in the inside spaces close to the open and sealed ends of filter cartridges, respectively, and the variation of static pressure was observed in the radial cross section of filter cartridges was negligible. For cone pleated filter cartridges with a low cone height HC (i.e., < 660 mm, the cartridge height), a negative pressure zone was found in the inside region near the open end of cartridges. Two events of pressure waves were experienced during the cleaning of cone pleated filter cartridges. Between both wave events, the first one was significantly affected by HC. As the HC was increased, the overall cleaning intensity developed inside the cartridges was topped for filters with HC = 760 mm and then decreased. In addition, the cleaning uniformity of cone pleated filter cartridges was generally improved compared to to that for ones without pleated filter cones.

Demonstration of opposing thermal sensitivities in hollow-core fibers with open and sealed ends.

The output phase and propagation time of an optical signal propagating through a hollow-core optical fiber (HCF) drift with changes in environmental temperature significantly less than in conventional optical fibers. In all earlier experimental studies, however, the simplifying assumption was made that the thermo-optic effect of air was negligible. In this Letter, we present, to the best of our knowledge, the first experimental demonstration that the air inside a HCF core can make an appreciable contribution to the fiber's thermal sensitivity with the performance depending on whether the fiber is open to the atmosphere or sealed at both ends (e.g., spliced to solid fiber pigtails). We measure both the sensitivity of the accumulated phase as well as the signal propagation time for both open and sealed HCF and show that these are opposite in sign. Most importantly, we show that the thermal sensitivity contribution from the air inside an open HCF has the sign opposite to the effect of fiber elongation (which is otherwise the dominant effect responsible for the overall thermal sensitivity of HCF). We then go on to show that these two effects can be used to balance each other out in order to achieve zero thermal sensitivity for both accumulated phase and propagation time. We demonstrate this property experimentally over a large spectral range.

Mechanical experiment evaluation of the microvascular self-healing capability of bitumen using hollow fibers containing oily rejuvenator

Smart microvascular self-healing bituminous composites are considered a promising approach to prolong pavement service life. The objective of this work was to investigate the microvascular self-healing capability of bituminous material by using hollow fibers with oily rejuvenator through a direct-tension mechanical experiment. The hollow fibers with oily rejuvenator were prepared using a wet-spinning approach by applying polyvinylidene fluoride material. The fixed-length hollow fibers with sealed ends were mixed in bitumen to form composite samples. Through a repetitive tensile method, the tension strength data were used to calculate the self-healing efficiency autonomously by considering crack closure and healing at various healing temperatures and times. The scanning-electron-microscope morphology showed that the polyvinylidene fluoride hollow fibers had a tight interfacial structure with bituminous material without debonding. X-ray computed tomography results indicated that the fibers were distributed homogeneously in bitumen. The fiber content and fiber orientation affected the self-healing capability of bituminous samples at 0 °C. To simplify the influence of the above two factors of the fibers, only one fiber was placed in bitumen samples parallel to the tension direction. With an increase in temperature from 0 to 30 °C, the self-healing capability of the bitumen samples increased dramatically. This phenomenon is attributed to the accelerated penetration speed of rejuvenator in the bitumen. An increase in time increased the self-healing capability of the bitumen samples. The rejuvenator may have sufficient time to leak from the hollow fibers and penetrate the bitumen. The results provide a guide to the microstructural design of the vascular fibers and the application of hollow fibers in self-healing asphalt pavement.

Hydrodynamic self-lubricating journal bearings analysis using Rabinowitsch fluid lubricant

Abstract In this study, the effects of non-Newtonian pseudo-plastic lubricants behavior, using Rabinowitsch fluid model, on static characteristics of circular self-lubricating porous journal bearings of finite length with sealed ends are highlighted. The nonlinear Reynolds equation based on the Rabinowitsch fluid model has been developed specifically for porous journal bearing cases by considering the fluid flow in the porous matrix described by Darcy's law. The Governing equations are discretized using a centered finite difference scheme and solved numerically using an iterative method. Following to the absence of the porous matrix or the non-Newtonian fluid behavior, the obtained results showed a good agreement with those of the literature review. The static characteristics of hydrodynamic lubrication are calculated numerically for various parameters such as bearing dimensions; the porous bush permeability and the lubricant pseudo-plastic coefficient. It is shown that the dimensionless permeability has significant effects on the static characteristics of the bearings. The pseudo-plastic lubricant coefficient decreases the bearing characteristics (load capacity, pressure) compared to Newtonian lubricant cases. The use of the pseudo-plastic fluids reduces the performance of the bearings and this reduction grows with the porous bearing properties.

ABOUT FEATURES OF SETTING AND QUALITY OF MEALS OF BOARD FEED IN THE CONDITIONS OF CATERING «KOLTSOVO»

Onboard food is one of the components of normal flight tolerance. At enterprises for the production of on-board meals, ready meals and culinary products are produced, and their sale and consumption are organized. The purpose of the research was to study the features of manning and the quality of meals onboard food in the conditions of «Catering «Koltsovo» (the city of Yekaterinburg). As an object of research, a meat snack from the meals of the economy class food was used. In the course of physical and chemical tests, it has been established that the quality of raw materials (cheese of Dutch, boiled-smoked sausage and corn canned) used to make snacks, the content of table salt, moisture, foreign impurities, toxic metals (cadmium, lead) Regulated requirements. At the enterprise under study every technological stage is taken: storage, preparation, bundling of onboard food. On-board meals are prepared using high-tech equipment of Russian, Italian and German companies with observance of the temperature regime. Examination of the on-board dish revealed that the meat snack was placed in a disposable plate and packed in a polymer film with sealed ends from both sides. The label is marked on the package, the label contains the date of manufacture of the snack, the flight number of the aircraft and the shift. Organoleptic parameters of meat snacks, as well as sodium chloride content, average dish mass meet the requirements of the current regulatory documents. The onboard food prepared in conditions «Catering «Koltsovo», distinguish excellent taste qualities and attractive appearance.

Experimental observation of the vascular self-healing hollow fibers containing rejuvenator states in bitumen

Hollow fiber containing rejuvenator is a promising smart material for vascular self-healing bituminous materials of pavement. The objective of this work was to experimentally observe the states of self-healing hollow fibers containing rejuvenator in pure bituminous material. Polyvinylidene fluoride (PVDF) hollow fibers containing oily rejuvenator were spun through single-wet-spinning method. The fixed-length hollow fibers with sealed ends were mixed in bitumen forming composite samples. The inter-surface and outer-surface microstructure of hollow fibers were observed by an environmental scan electron microscopy (ESEM). The micropores in fiber-shells were channels for rejuvenator to pass through. X-ray computed tomography (XCT) results indicated that these fibers distributed in bitumen with an integrality state. Three-dimensional reconstruction XCT (3D-XCT) images exhibited the details both of vertical and horizontal distribution of hollow fibers in bitumen. Thermogravimetic analysis (TGA) and a repeated thermal-cycle testament showed that the hollow fibers survived safely in bitumen. No debonding phenomenon was found between the fibers and bitumen. Oily rejuvenator released out of hollow fibers smoothly through the break point of hollow fiber/bitumen composite samples. The break images of hollow fibers in bitumen were also analyzed through 3D-XCT structures. At the same time, the movement tracks of oily rejuvenator out of hollow fibers were observed by a fluorescence microscopy. The rejuvenator permeated through the micropores on a hollow fiber into aged bitumen. These states mean that the self-healing can be triggered through the break points of hollow fibers as well as the self-rejuvenation when bitumen is aging. The above result is evidence that the hollow fibers containing rejuvenator will be a feasible approach to realize the self-healing and self-rejuvenating process of bitumen.

Experimental Force Coefficients for Two Sealed Ends Squeeze Film Dampers (Piston Rings and O-Rings): An Assessment of Their Similarities and Differences

Squeeze film dampers (SFDs) in aircraft engines effectively aid to reduce rotor motion amplitudes, in particular when traversing a critical speed, and help to alleviate rotor whirl instabilities. The current work is a long-term endeavor focused on quantifying the dynamic force performance of practical SFDs, exploring novel design damper configurations, and producing physically sound predictive SFD models validated by experimental data. Piston rings (PRs) and O-rings (ORs), commonly used as end seals in SFDs for commercial and military gas turbine engines, respectively, amplify viscous damping in a short physical length and while operating with a modicum of lubricant flow. This paper presents experimental force coefficients (damping and inertia) for two identical geometry SFDs with end seals, one configuration hosts PRs, and the other one ORs. The test rig comprises a stationary journal and bearing cartridge (BC) hosting the SFD and supported on four elastic rods to emulate a squirrel cage. The damper film land length, diameter, and clearance are L = 25.4 mm, D = 5L, and c = 0.373 mm (D/c = 340), respectively. A supply feeds ISO VG 2 oil to the film land at its middle plane through either one hole or three holes, 2.5 mm in diameter, 120 deg apart. In the PRSFD, the lubricant exits through the slit opening at the ring butted ends. The ORs suppress oil leakage; hence, lubricant evacuates through a 1 mm hole at ¼ L near one journal end. The ORs when installed add significant stiffness and damping to the test structure. The ORSFD produces 20% more damping than the PRSFD, whereas both sealed ends SFDs show similar size added mass. For oil supplied at 0.69 bar(g) through a single orifice produces larger damping, 60–80% more than when the damper operates with three oil feedholes. A computational model reproducing the test conditions delivers force coefficients in agreement with the test data. Archival literature calls for measurement of a single pressure signal to estimate SFD reaction forces. For circular centered orbits (CCOs), the dynamic pressure field, in the absence of any geometrical asymmetry or feed/discharge oil condition, “rotates” around the bearing with a speed equal to the whirl frequency. The paper presents force coefficients estimated from (a) measurements of the applied forces and ensuing displacements, and (b) the dynamic pressure recorded at a fixed angular location and “integrated” over the journal surface. The first method delivers a damping coefficient that is large even with lubricant supplied at a low oil supply pressure whereas the inertia coefficient increases steadily with feed pressure. Predictions show good agreement with the test results from measured forces and displacements, in particular the added mass. On the other hand, identified damping and inertia coefficients from dynamic pressures show a marked difference from one pressure sensor to another, and vastly disagreeing with test results from the first method or predictions. The rationale for the discrepancy relies on local distortions in the dynamic pressure fields that show zones of oil vapor cavitation at a near zero absolute pressure and/or with air ingestion producing high frequency spikes from bubble collapsing; both phenomena depend on the magnitude of the oil supply pressure. An increase in lubricant supply pressure suppresses both oil vapor cavitation and air ingestion, which produces an increase of both damping and inertia force coefficients. No prior art compares the performance of a PRSFD vis-à-vis that of an ORSFD. Supplying lubricant with a large enough pressure (flow rate) is crucial to avoid the pervasiveness of air ingestion. Last, the discussion on force coefficients obtained from two distinct methods questions the use of an oversimplifying assumption; the dynamic pressure field is not invariant in a rotating coordinate frame.

Asymmetric Sensory-Motor Regeneration of Transected Peripheral Nerves Using Molecular Guidance Cues

Neural interfaces are designed to decode motor intent and evoke sensory precepts in amputees. In peripheral nerves, recording movement intent is challenging because motor axons are only a small fraction compared to sensory fibers and are heterogeneously mixed particularly at proximal levels. We previously reported that pain and myelinated axons regenerating through a Y-shaped nerve guide with sealed ends, can be modulated by luminar release of nerve growth factor (NGF) and neurotrophin-3 (NT-3), respectively. Here, we evaluate the differential potency of NGF, glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), pleiotrophin (PTN), and NT-3 in asymmetrically guiding the regeneration of sensory and motor neurons. We report that, in the absence of distal target organs, molecular guidance cues can mediate the growth of electrically conductive fascicles with normal microanatomy. Compared to Y-tube compartments with bovine serum albumin (BSA), GDNF and NGF increased the motor and sensory axon content, respectively. In addition, the sensory to motor ratio was significantly increased by PTN (12.7:1) when compared to a BDNF + GDNF choice. The differential content of motor and sensory axons modulated by selective guidance cues may provide a strategy to better define axon types in peripheral nerve interfaces.

Effects of catalyst agglomerate shape in polymer electrolyte fuel cells investigated by a multi-scale modelling framework

A multi-scale modelling framework is developed for the PEFC cathode electrode. Unlike the conventional agglomerate model, the effects of the microstructure of the agglomerate are numerically coupled to the fuel cell-scale model in this framework. This is performed through solving the agglomerate-scale model first and subsequently extracting and using the data required to generate the performance curves in the fuel cell-scale model. This enables one to freely investigate the structure of the agglomerate without being limited to the only three agglomerate shapes that can be investigated using the conventional agglomerate model: spheres, long cylinders with sealed ends and long slabs with sealed ends. The numerical studies conducted in this work using the developed framework have revealed that the performance of the cathode electrode is highly sensitive to the specific surface area of the agglomerate if the size of the latter is relatively large, i.e. of the order of 1000 nm. Namely, the maximum reported current density has increased by about 60% when changing from the ‘large’ spherical agglomerate to the ‘large’ cylindrical agglomerate. Also, it has been shown that a slight change in the structure of the agglomerate may significantly improve the fuel cell performance.

Hemodialyzer Reuse and Gram-Negative Bloodstream Infections

Clusters of bloodstream infections caused by Burkholderia cepacia and Stenotrophomonas maltophilia are uncommon, but have been previously identified in hemodialysis centers that reprocessed dialyzers for reuse on patients. We investigated an outbreak of bloodstream infections caused by B cepacia and S maltophilia among hemodialysis patients in clinics of a dialysis organization. Outbreak investigation, including matched case-control study. Hemodialysis patients treated in multiple outpatient clinics owned by a dialysis organization. Main predictors were dialyzer reuse, dialyzer model, and dialyzer reprocessing practice. Case patients had a bloodstream infection caused by B cepacia or S maltophilia; controls were patients without infection dialyzed at the same clinic on the same day as a case; results of environmental cultures and organism typing. 17 cases (9 B cepacia and 8 S maltophilia bloodstream infections) occurred in 5 clinics owned by the same dialysis organization. Case patients were more likely to have received hemodialysis with a dialyzer that had been used more than 6 times (matched OR, 7.03; 95% CI, 1.38-69.76) and to have been dialyzed with a specific reusable dialyzer (Model R) with sealed ends (OR, 22.87; 95% CI, 4.49-∞). No major lapses during dialyzer reprocessing were identified that could explain the outbreak. B cepacia was isolated from samples collected from a dialyzer header-cleaning machine from a clinic with cases and was indistinguishable from a patient isolate collected from the same clinic, by pulsed-field gel electrophoresis. Gram-negative bacteria were isolated from 2 reused Model R dialyzers that had undergone the facility's reprocessing procedure. Limited statistical power and overmatching; few patient isolates and dialyzers available for testing. This outbreak was likely caused by contamination during reprocessing of reused dialyzers. Results of this and previous investigations demonstrate that exposing patients to reused dialyzers increases the risk for bloodstream infections. To reduce infection risk, providers should consider implementing single dialyzer use whenever possible.