Resin Sealer Research Articles

Effect of Sodium Hypochlorite Concentration in Continuous Chelation on Dislodgement Resistance of an Epoxy Resin and Hydraulic Calcium Silicate Sealer.

The conditioning of the root canal wall during chemo-mechanical root canal treatment differentially affects the adhesion of root canal sealers. This investigation evaluated the impact of sodium hypochlorite (NaOCl) concentration as used in a root canal irrigation concept called continuous chelation, with 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) contained in the NaOCl solution that is applied. Fourier-transform infrared spectra of the dentinal wall were gathered. The consequential effects on push-out bond strength of an epoxy resin (AH Plus) versus a hydraulic CaSi sealer (BioRoot RCS) were assessed. Single-rooted extracted human teeth were used and irrigated with pure NaOCl at a concentration of 0% (physiological saline), 2.5%, or 5.25%. Dual Rinse HEDP (9%) was added to the solutions, or not added for further control. Pure NaOCl solutions caused a decrease in the amide III: phosphate ratios, which was counter-acted by the addition of HEDP. It was observed that the adhesion of the epoxy resin sealer under investigation was negatively affected by this NaOCl deproteinization of the canal wall in a dose-dependent manner, while the opposite was observed with the CaSi sealer. HEDP when used in conjunction with NaOCl was beneficial for the adhesion of both sealers.

Adding Two Antimicrobial Glasses to an Endodontic Sealer to Prevent Bacterial Root Canal Reinfection: An In Vivo Pilot Study in Dogs.

Current endodontic procedures continue to be unsuccessful for completely removing pathogens present inside the root canal system, which can lead to recurrent infections. In this study, we aimed to assess the antimicrobial capacity and tissue response of two inorganic bactericidal additives incorporated into a paste root canal sealer on contaminated root dentin in vivo. An experimental study was performed in 30 teeth of five Beagle dogs. After inducing microbiological contamination, root canal systems were treated by randomly incorporating one of two antimicrobial additives into a commercial epoxy-amine resin sealer (AH Plus), i.e., G3T glass-ceramic (n = 10) and ZnO-enriched glass (n = 10); 10 samples were randomized as a control group. After having sacrificed the animals, microbiological, radiological, and histological analyses were performed, which were complemented with an in vitro bactericidal test and characterization by field emission scanning electron microscopy. The tested groups demonstrated a non-significant microbiological reduction in the postmortem periapical index values between the control group and the bactericidal glass-ceramic group (p = 0.885), and between the control group and the ZnO-enriched glass group (p = 0.169). The histological results showed low values of inflammatory infiltrate, and a healing pattern characterized by fibrosis in 44.4% of the G3T glass-ceramic and 60.0% of ZnO-enriched glass. Bactericidal glassy additives incorporated in this root canal sealer are safe and effective in bacterial reduction.

Minimal Dentinal Tubule Penetration of Endodontic Sealers in Warm Vertical Compaction by Direct Detection via SEM Analysis.

Sealer staining using rhodamine B dye to investigate the penetration depth of endodontic sealers was proven unsuitable for this purpose. This study aimed to investigate the sealer penetration depth into dentinal tubules by scanning electron microscopy (SEM). Root canals of 52 human upper central incisors were instrumented using the ProTaper Gold NiTi system (Dentsply Sirona, York, PA, USA) up to size F3. After irrigation with sodium hypochlorite and citric acid combined with ultrasonic activation, the root canals were either filled using the epoxy resin sealer AH Plus (Dentsply Sirona) or the calcium silicate-based sealer Total Fill BC Sealer HiFlow (TFHF, FKG Dentaire, La Chaux-de-Fonds, Switzerland) by warm vertical compaction. Root slices of 1 mm thickness were obtained at 2 to 3, 5 to 6 and 8 to 9 mm from the apex. The root slices were investigated for sealer penetration into the dentinal tubules using SEM according to four root quadrants (buccal, mesial, oral, distal). Statistical analysis was performed by the Kruskal-Wallis test (p = 0.05) as data were not normally distributed according to the Shapiro-Wilk test. AH Plus penetrated significantly deeper into the dentinal tubules compared to TFHF at each root level (p < 0.05). Dentinal sealer penetration was deeper in the bucco-oral direction compared to the mesio-distal direction. AH Plus penetrated deeper into dentinal tubules than TFHF. Warm vertical compaction exerting high pressure on the root canal filling material is not able to press sealers deep into dentinal tubules as penetration depth values did not exceed a mean of 110 µm in SEM.

Evaluation Of Commonly Treated Maxillary Teeth With Preventive Resin Sealant Among Children With Mixed Dentition

Evaluation Of Commonly Treated Maxillary Teeth With Preventive Resin Sealant Among Children With Mixed Dentition

Evaluation Of Commonly Treated Maxillary Teeth With Preventive Resin Sealant Among Children With Primary Dentition

Evaluation Of Commonly Treated Maxillary Teeth With Preventive Resin Sealant Among Children With Primary Dentition

Evaluation Of Commonly Treated Mandibular Teeth With Preventive Resin Sealant Among Children With Permanent Dentition

Evaluation Of Commonly Treated Mandibular Teeth With Preventive Resin Sealant Among Children With Permanent Dentition

Evaluation Of Commonly Treated Mandibular Teeth With Preventive Resin Sealant Among Children With Mixed Dentition

Evaluation Of Commonly Treated Mandibular Teeth With Preventive Resin Sealant Among Children With Mixed Dentition

An Efficacy Study between High Viscosity Glass Ionomers and Resin Sealants in Fissure Caries Prevention: A 2-Year Split Mouth Randomized Controlled Trial.

Objectives This clinical trial compared the efficacy of atraumatic restorative treatment (ART) sealants against resin-based sealants in terms of their retention and fissure caries preventive benefits over a period of 24 months among a section of school children in the Southern Indian state of Telangana. Materials and Methods A split mouth clinical trial employed 198 children, who received these sealants on their lower permanent first molars. Retention was assessed 6 monthly and caries annually Statistical Analysis Chi-square tests were utilized to analyze the retention rate and the incidence of dental caries between the two groups. Kaplan–Meier survival analysis plotted the cumulative survival percentage of partially, and fully retained sealants and the survival of dentin carious free pits and fissures among both the groups. A linear binary logistical regression analysis calculated the odds ratio. Results A statistical significant difference was observed in the retention rate between these sealants at every follow-up stage. The cumulative survival percentage of ART and resin sealants was calculated to be 30.9 and 37.5% by the end of 2 years. The Kaplan–Meier analysis showed no significant difference with regard to the survival of dentin carious free pits and fissures. The odds ratio for this trial was 0.747 (95% confidence interval: 0.493–1.13) Conclusion Resin sealants fared better than ART sealants in the field of retention. However, no significant differences were observed with regard to fissure caries prevention by the end of the study period.

Assessment of Root Canal Sealers Loaded with Drug-Silica Coassembled Particles Using an In Vitro Tooth Model

IntroductionThe purpose of this study was to assess the antimicrobial activity of root canal sealers modified with novel highly loaded antimicrobial drug-silica coassembled particles (DSPs) on Enterococcus faecalis–infected root canal dentin. MethodsDSPs were synthesized through coassembly of silica and octenidine dihydrochloride (OCT) surfactant drug (35% w/w OCT). DSPs (1% wt of the total mass of the sealer) were mixed homogenously with either epoxy resin sealer (AH Plus [AH]; Dentsply Sirona, Tulsa, OK) or calcium silicate–based sealer (EndoSequence BC Sealer [BC]; Brasseler, Savannah, GA). To assess the antimicrobial activity of DSP-loaded sealers, the apical third of single-rooted teeth was obtained and infected with E. faecalis for 3 weeks followed by the application of experimental (DSP-loaded) sealers or corresponding controls for up to 28 days. Microbiological analysis and laser scanning confocal and scanning electron microscopy were used to determine the colony-forming unit (CFU)/mL, the percentage of live bacteria, and the intratubular bacterial and sealer penetrations. Factorial analysis of variance and Tukey post hoc tests were used to assess the antimicrobial effect of DSPs on different sealers. ResultsAll experimental groups showed significant reductions in CFUs at all-time points compared with positive controls (P < .05). The addition of DSPs to BC significantly reduced the CFUs (2.11 ± 0.13, 2.22 ± 0.19, and 2.25 ± 0.17 at 1, 7, and 28 days, respectively) compared with the unmodified sealer (3.21 ± 0.11, 4.3 ± 0.15, and 4.2 ± 0.2 at 0, 7, and 28 days). DSPs enhanced the antimicrobial performance of AH only at 1 day (4.21 ± 0.17 vs 5.19 ± 0.12, P < .05). AH and AH + DSPs showed higher bacterial viability compared with BC and BC + DSPs at all incubation periods (P < .05). ConclusionsLoading endodontic sealers with DSPs had a material-dependent effect on the antimicrobial properties and could reduce the incidence of secondary infections.

Teaching Minimally Invasive Interventions in Paediatric Dentistry: A Cross-Sectional Survey of Dental Schools in Iran

BackgroundDental caries is a significant public health problem in Iran. Teaching minimally invasive interventions in paediatric dentistry may facilitate the provision of treatment for untreated dental caries in children. We evaluated the teaching of such interventions in both undergraduate dental curriculum and Paediatric Dentistry Specialty Training Programme (PDSTP) in Iran.MethodsThis was a cross-sectional questionnaire-based survey. Participants in this study were the directors of 40 undergraduate programmes and 15 PDSTPs in all Iranian dental schools (response rate = 100%). Descriptive statistics were reported.ResultsThe most commonly taught methods were preventive fissure sealant and preventive resin restoration (PRR), which were taught ‘both didactically and clinically’ in all undergraduate dental programmes. The least commonly taught methods were silver diamine fluoride (SDF), the Hall technique and resin infiltration, which were taught ‘both didactically and clinically’ in less than 5% of dental schools. The same three methods were the least commonly approaches taught in PDSTP, further, they were less often perceived to be ‘essential’.ConclusionsThere was a notable variation in the teaching of the management of dental caries in Iran’s dental education. Some minimally invasive approaches including SDF, the Hall technique and resin infiltration are not being commonly taught in Iranian dental schools despite the evidence base for these techniques.

Dentinal tubule penetration of gutta-percha with syringe-mix resin sealer using different obturation techniques: A confocal laser scanning microscopy study.

This study aimed to evaluate dentinal tubule penetration of the root canal filling material using various techniques such as cold lateral compaction, new generation thermoplastic core carrier and continuous-wave obturation. The root canals of premolar teeth were instrumented, and the teeth were allocated randomly to three groups (n = 15/group). After the obturation with three different techniques, three sections of 2-mm thickness at 2, 5 and 8-mm from the apex were examined under a confocal laser scanning microscope. The data were analysed of variance with a significance level of P < 0.05. There was no significant difference between techniques when parameters were evaluated in total (penetration depth P = 0.418, penetration area P = 0.701). The mean value of the cold lateral compaction group for maximum penetration depth was obtained higher than continuous wave (P = 0.004), whilst there was no significant difference between the thermoplastic core carrier and the other groups.

The Effect of Obturation Technique on the Fracture Resistance of Endodontically Treated Teeth Obturated with Bioceramic Sealer. (In Vitro Study)

Aim of the study: This study evaluated the effect of obturation technique on the fracture resistance of endodontically treated teeth obturated with bioceramic sealers compared to resin-based sealers. Methods: Ninety-one (n=91) freshly human extracted single rooted teeth were selected. The samples were biomechanically prepared and classified according to the obturation technique and type of the sealer used into five groups as follows: Group 1 (n=21) obturated with cold lateral compaction technique using gutta-percha and AD-seal sealer (Meta Biomed, Korea). Group 2 (n=21) obturated with warm vertical compaction technique using gutta-percha and AD-seal sealer. Group 3 (n=21) obturated with cold lateral compaction technique using gutta-percha and MTA filapex sealer (Angelus, Brazil). Group 4 (n=21) obturated with warm vertical compaction technique using gutta-percha and MTA filapex sealer. Group 5 (n=7) Control group (unobturated). The samples were further classified according to the observation time into: subgroup A (n=7) evaluated after one week, subgroup B (n=7) evaluated after two weeks and subgroup C (n=7) evaluated after one month. Samples were loaded vertically after obturation using Universal Testing Machine until fracture. Results: MTA filapex group showed higher fracture resistance values than the other groups with no significant difference. No significant difference was found between the subgroups evaluated at different time intervals. Conclusion: The technique of root canal obturation didn’t significantly affect tooth fracture resistance of either teeth obturated with Bio-ceramic sealer or resin sealer at different time intervals.

Formation of Cariogenic Bacterial Biofilm on Vanillin-Incorporated Resin-Based Dental Sealant

Tooth decay or dental caries is an important oral health problem involving people of all age groups. The disease is the outcome of the demineralize process in which aciduric and acidogenic bacteria in a biofilm decompose tooth structure. Dental sealant, a resin material, which is applied on the occlusal pit and fissure surfaces of the teeth as a protective layer has been commonly used to prevent dental caries. However, the microbial effect on food residue is found to be a major cause of microleakage of sealant and secondary caries. Several types of antimicrobial agents were introduced to increase the caries preventive effect of dental sealants. Vanillin, the main component of flavoring agent vanilla, has been found to have antimicrobial property against Gram-positive and Gram-negative bacteria. The objective of this study was to investigate the antimicrobial effect of vanillin-incorporated dental sealant against biofilm formation of cariogenic bacteria. Dental sealant resin samples (Clinpro; 3M ESPE, USA) were prepared in 96-well plate in accordance with the amount of vanillin adding (0%, 0.5%, 1% and 5% vanillin). The cariogenic bacterial suspensions of Streptococcus mutans ATCC 25175 and Lactobacillus casei ATCC 334 were added to saliva-coated samples and incubated at 37°C in 5% CO2 atmosphere for 48 h to allow the biofilm formation. The quantity of vital biofilm was determined by WST Microbial Cell Counting Kit (Dojindo Molecular Technologies, USA) at 460 nm. One-way ANOVA and Tukey’s test were applied to the statistical analysis. A significant inhibitory effect against L. casei biofilm was observed in all vanillin incorporated samples (0.5%, 1% and 5% vanillin) compared with samples without vanillin. The percentage of biofilm reduction was 32-39%. For S. mutans, the suppressive effect was noticed only at &gt;1% vanillin with 18-25% biofilm reduction. In conclusion, the incorporation of vanillin to dental sealants could decrease biofilm formation of cariogenic bacteria (S. mutans and L. casei). The use of dental sealants containing vanillin could be a promising measure to prevent dental caries due to their antibacterial biofilm formation property.

Debunking the Concept of Dentinal Tubule Penetration of Endodontic Sealers: Sealer Staining with Rhodamine B Fluorescent Dye Is an Inadequate Method.

The aim of this study was to investigate the suitability of rhodamine B dye staining of an epoxy resin sealer (AH Plus) and calcium-silicate-based sealers (Total Fill BC Sealer, BioRoot RCS) to represent the penetration depth of the sealers into dentinal tubules after root canal obturation. In a three-step process, (1) leaching of rhodamine B from sealers into a buffer solution, (2) passive penetration of leached rhodamine B into dentinal tubules, and (3) conformity of rhodamine B penetration assessed by confocal laser scanning microscopy (CLSM), and sealer penetration assessed by scanning electron microscopy (SEM), in root-canal-filled teeth, were evaluated. Rhodamine B dye massively leached out of Total Fill BC Sealer and BioRoot RCS into the phosphate-buffered saline (PBS). A pinkish coloration of AH Plus was found after contact with PBS. Leached rhodamine B dye passively penetrated dentinal tubules from all three sealers when placed on root dentin. No correlation was observed between sealer penetration in SEM and rhodamine B penetration in CLSM. Staining of sealers using rhodamine B is an inadequate method with which to evaluate sealer penetration depth into dentinal tubules, as it overestimates the penetration of sealers into root dentin tubules.

PERBEDAAN EFEKTIVITAS PELARUT ORANGE OIL TERHADAP SEALER BERBASIS RESIS DAN ZINC OXIDE EUGENOL

This study aims to determine the differences in the effectiveness of orange oil solvent against resin-based sealers and zinc oxide eugenol. The samples of this study were resin based sealers and zinc oxide eugenol based sealers which were molded in a cylindrical mold made of aluminum with a diameter of 10 mm and a height of 3 mm. The total sample size in this study was 60 samples, then divided into 4 groups, 2 groups for resin-based sealers with a time of 2 minutes and 5 minutes, and a sealer based on zinc oxide eugenol with a time of 2 minutes and a time of 5 minutes, after which it was put into incubator with a temperature of 370C for 48 hours, then weighed for each group. Data analysis used paired T test with the Kolmogorov Smirnov normality test. The results showed that the orange oil solvent did not have a significant difference to the resin-based sealers and the zinc oxide eugenol-based sealers. At the immersion time of 2 minutes, the solubility of resin-based sealers and zinc oxide eugenol did not have significant differences (p&gt; 0.05). Based on the research results, it can be concluded that orange oil solvent is equally effective in both sealers, neither is more effective than the other&#x0D; Keywords: Orange Oil solvent, resin sealer, Zinc Oxide Eugenol sealer.&#x0D;

Comparative Evaluation of Fracture Resistance of Root Dentine of Two MTA based Sealers and a Resin Sealer - An In Vitro Study

Comparative Evaluation of Fracture Resistance of Root Dentine of Two MTA based Sealers and a Resin Sealer - An In Vitro Study

A cost minimisation analysis of two dental sealing methods in an outreach dental program

ObjectiveTo conduct economic evaluation of two sealing methods (placement of resin sealant or glass ionomer ART sealant) in preventing pit and fissure caries in permanent first molars (PFMs) in schoolchildren. Materials and methodsAn outreach dental program was conducted in two randomly selected primary schools, one was covered by the national government-funded dental sealing service (group 1) and the other was not (group 2). Resin or ART sealant (sealant using glass ionomer material with the aid of the finger-press technique) was applied to all of the eligible PFMs in group 1 and group 2 students (grade 1 or 2), respectively. All the applications was provided at school. The cost for sealant application was recorded for each method. After 18 months, a cost minimisation analysis was performed based on the efficacy results of the two methods. Results and conclusionIn total, 445 sealants were applied to 126 children in group 1, and 668 sealants were applied to 203 children in group 2. Dental caries incidence was 29.4 % in group 1 and 28.6 % in group 2 after 18 months. Logistic regression analysis showed no statistically significant difference (p = 0.552) between the two groups. Cost minimisation analysis was used to evaluate the economic performance. The analytical model showed that the ART sealant was economically better than resin sealant. Sensitivity analysis showed that the life expectancy of the equipment/instrument used in sealant applications was the major parameter affecting the cost. The cost of using ART sealant over the study period was lower than that of using resin sealant. Overall, ART sealant is a less costly alternative for pit and fissure caries prevention in outreach dental services. Clinical significanceWhile pit and fissure sealing using ART sealant was as effective as using resin sealant in dental caries prevention, the cost of using ART sealant was lower than that of using resin sealant. Therefore, ART sealant could be a good alternative to resin sealant for pit and fissure caries prevention in outreach dental services for rural populations who have the greatest need.

Effect of phytic acid and ethylenediaminetetraacetic acid on penetration depth of bioceramic and resin sealers.

This study assessed influence of 1% phytic acid and 17% ethylenediaminetetraacetic acid (EDTA) on the dentinal tubules penetration of EndoSequence BC bioceramic-based and AH Plus resin-based sealers using confocal laser scanning microscopy (CLSM). Forty single-rooted mandibular premolars were divided randomly into four groups (n = 10). Group 1 received final irrigation with 1% phytic acid solution and canals sealed by AH Plus sealer, Group 2: 1% phytic acid solution and EndoSequence BC sealer, Group 3: 17% EDTA solution and AH Plus sealer and Group 4: 17% EDTA solution and EndoSequence BC sealer. Specimens were horizontally sectioned 2, 4 and 6 mm from the apex. Average maximum depth of sealer penetration was examined using CLSM. Group three showed the deepest sealer penetration at all three levels which was significantly higher than all other groups (P ˂ 0.05). Deep sealer penetration is achievable with AH Plus and EDTA. Phytic acid groups have moderate tubular penetration ability.

What’s new for the clinician? - Excerpts from and summaries of recently published papers

Choosing an endodontic sealer clinical use is a decision that contributes to the long-term success of non-surgical root canal treatment. Sealers are used as a thin tacky paste which function as a lubricant and luting agent during obturation, allowing the core obturation material, such as gutta-percha points or other rigid materials, to slide in and become fixed in the canal. Sealers can fill voids, lateral canals, and accessory canals where core obturation materials cannot infiltrate. If the sealer does not perform its function, microleakage may cause root canal failure via clinically undetectable passage of bacteria, fluids, molecules or ions between the tooth and restorative material. It has been reported that extrusion of the sealer during root canal filling has cytotoxic effects on periapical tissues, causing periapical inflammation, necrosis and pain. Endodontic sealers are categorized by composition based on setting reaction and composition: zinc oxide eugenol, salicylate, fatty acid, glass ionomer, silicone, epoxy resin, tricalcium silicate, and methacrylate resin sealer systems. Aslan &amp; Özkan (2021) reported on a trial that sought to evaluate the effect of two calcium silicate-based root canal sealers, Endoseal MTA and EndoSequence BC Sealer, on postoperative pain following single-visit root canal treatment on molar teeth compared to their epoxy/ amine resin-based counterpart AH Plus. The null hypotheses tested in this study were as follows:1. The type of sealer used would not change the incidence and the intensity of post-treatment endodontic pain2. The analgesic intake of patients following single-visit root canal treatment

Pit and Fissure Sealants as a Viable Preventive Tool for Dental Caries

Dental caries remains to be a global disease burden and the current ways of preventing it are throughmaintenance of good oral hygiene, watching over the diet intake and pit and fissure sealant application. Pitand fissure sealants are still underused in dental practice. With the availability of the various type of sealantsranging from glass ionomer sealants to resin sealants, we need to improvise our preventive protocols fordental caries. This article provides crisp and compact information on the current status and usage of dentalsealants to encourage the fraternity to use it more often.