Sea Urchin Lytechinus Variegatus Research Articles

Leachates of weathering plastics from an urban sandy beach: Toxicity to sea urchin fertilization and early development

Plastic leachates have chemical and biological implications for marine environments. This study experimentally evaluated acute effects of weathering plastic leachates (0, 25, 50, 75 and 100 %) on fertilization and early development of the sea urchin Lytechinus variegatus. Fertilization, embryonic and larval development were drastically inhibited (~75 %) when gametes were exposed to intermediate and high leachate concentrations or delayed when exposed to the lowest concentration. Fertilization and first cleavage stages were highly affected by exposure to intermediate and high leachate concentrations. None of the cells incubated at concentrations from 50 % reached blastula stage, suggesting that embryonic development was the most sensitive stage. Abnormalities in embryos and larvae were observed in all leachate treatments. Chemical analysis detected high concentration of bisphenol A, which may induce these observed effects. Our results highlight the potential threats of plastic pollution to sea urchin populations, which may severely affect the structure and functioning of coastal ecosystems.

The dopamine receptor antagonist haloperidol disrupts behavioral responses of sea urchins and sea stars.

Despite lacking a brain and having an apparent symmetrically pentaradial nervous system, echinoderms are capable of complex, coordinated directional behavioral responses to different sensory stimuli. However, very little is known about the molecular and cellular mechanisms underlying these behaviors. In many animals, dopaminergic systems play key roles in motivating and coordinating behavior, and although the dopamine receptor antagonist haloperidol has been shown to inhibit the righting response of the sea urchin Strongylocentrotus purpuratus, it is not known whether this is specific to this behavior, in this species, or whether dopaminergic systems are needed in general for echinoderm behaviors. We found that haloperidol inhibited multiple different behavioral responses in three different echinoderm species. Haloperidol inhibited the righting response of the sea urchin Lytechinus variegatus and of the sea star Luidia clathrata. It additionally inhibited the lantern reflex of S. purpuratus, the shell covering response of L. variegatus and the immersion response of L. variegatus, but not S. purpuratus or L. clathrata. Our results suggest that dopamine is needed for the neural processing and coordination of multiple different behavioral responses in a variety of different echinoderm species.

Warming and grazing independently and interactively impact plant defenses and palatability

The ecological impacts of multiple stressors are hard to predict but important to understand. When multiple stressors influence foundation species, the effects can cascade throughout the ecosystem. Gulf of Mexico seagrass ecosystems are currently experiencing a suite of novel stressors, including warmer water temperatures and increased herbivory due to tropicalization and conservation efforts. We investigated the impact of warming temperatures and grazing history on plant performance, morphology, and palatability by integrating a mesocosm study using the seagrass Thalassia testudinum with feeding trials using the sea urchin Lytechinus variegatus. Warming temperatures negatively impacted T. testudinum tolerance traits, reducing belowground biomass by 34%, productivity by 74%, shoot density by 10%, and number of leaves per plant by 24%, and negatively impacted resistance traits through 13% lower toughness of young leaves and a trend for reduced leaf carbon:nitrogen. Lytechinus variegatus individuals preferred to consume plants grown under heated conditions, which supports findings of enhanced palatability. Simulated turtle grazing impacted more plant traits than grazing by other herbivores, potentially diminishing plant resilience to future disturbances through reduced rhizome non‐structural carbohydrate concentrations and increasing palatability through reduced fiber content and 23% lower leaf carbon:phosphorus. Simulated turtle, simulated parrotfish, and urchin grazing reduced leaf carbon:nitrogen by 11%, also potentially increasing nutritive value. Interactions between warming temperatures and grazers on plant traits were additive for 16 out of 19 response variables. However, the stressors non‐additively impacted the number of leaves per plant, fiber content, and epiphyte load. We suggest that the impacts of grazers on leaf turnover rate and leaf age may vary based on water temperature, potentially driving these interactions. Overall, increased temperatures and grazing pressure will likely reduce seagrass resilience, structure, and biomass, potentially impacting feedback systems and producing negative consequences for seagrass cover, associated species, and ecosystem services.

Alterations induced by titanium dioxide nanoparticles (nano-TiO2) in fertilization and embryonic and larval development of the tropical sea urchin Lytechinus variegatus

The release of nanomaterials into the environment is the cause of an emerging concern. Titanium dioxide nanoparticles (nano-TiO2) among the most produced nanomaterials, has been documented in marine coastal areas posing a threat on marine biota. Sea urchin embryos are recognized as suitable bioindicators in ecological risk assessment and recently for nanomaterials. This study investigated the impact of nano-TiO2 on fertilization, embryonic and larval development of the tropical sea urchin Lytechinus variegatus in a range of concentrations (0.005–5 μg/mL) which includes environmentally relevant ones. The behavior of nano-TiO2 in tropical natural seawater was determined by dynamic light scattering (DLS) and toxicity was evaluated through fertilization and embryotoxicity tests, and morphological/morphometric analyses of sea urchin's larvae. Limited toxicity was recorded for nano-TiO2 in tropical sea urchin embryos and larvae, except for effects at the gastrula stage at 0.005 μg/mL. Large agglomerates of nano-TiO2 (5 μg/mL) were observed adhering onto sea urchin larvae thus probably preventing nanoparticles uptake at the highest concentrations (>0.005 μg/mL). Environmental levels of nano-TiO2 are able to cause toxicity on tropical sea urchin L. variegatus embryos with potential consequences on populations and their ecological role in tropical coastal areas.

SPR Sensor-Based Analysis of the Inhibition of Marine Sulfated Glycans on Interactions between Monkeypox Virus Proteins and Glycosaminoglycans.

Sulfated glycans from marine organisms are excellent sources of naturally occurring glycosaminoglycan (GAG) mimetics that demonstrate therapeutic activities, such as antiviral/microbial infection, anticoagulant, anticancer, and anti-inflammation activities. Many viruses use the heparan sulfate (HS) GAG on the surface of host cells as co-receptors for attachment and initiating cell entry. Therefore, virion-HS interactions have been targeted to develop broad-spectrum antiviral therapeutics. Here we report the potential anti-monkeypox virus (MPXV) activities of eight defined marine sulfated glycans, three fucosylated chondroitin sulfates, and three sulfated fucans extracted from the sea cucumber species Isostichopus badionotus, Holothuria floridana, and Pentacta pygmaea, and the sea urchin Lytechinus variegatus, as well as two chemically desulfated derivatives. The inhibitions of these marine sulfated glycans on MPXV A29 and A35 protein-heparin interactions were evaluated using surface plasmon resonance (SPR). These results demonstrated that the viral surface proteins of MPXV A29 and A35 bound to heparin, which is a highly sulfated HS, and sulfated glycans from sea cucumbers showed strong inhibition of MPXV A29 and A35 interactions. The study of molecular interactions between viral proteins and host cell GAGs is important in developing therapeutics for the prevention and treatment of MPXV.

Antiviral activity of marine sulfated glycans against pathogenic human coronaviruses

Great interest exists towards the discovery and development of broad-spectrum antivirals. This occurs due to the frequent emergence of new viruses which can also eventually lead to pandemics. A reasonable and efficient strategy to develop new broad-spectrum antivirals relies on targeting a common molecular player of various viruses. Heparan sulfate is a sulfated glycosaminoglycan present on the surface of cells which plays a key role as co-receptor in many virus infections. In previous work, marine sulfated glycans (MSGs) were identified as having antiviral activities. Their mechanism of action relies primarily on competitive inhibition of virion binding to heparan sulfate, preventing virus attachment to the cell surface prior to entry. In the current work we used pseudotyped lentivirus particles to investigate in a comparative fashion the inhibitory properties of five structurally defined MSGs against SARS-CoV-1, SARS-CoV-2, MERS-CoV, and influenza A virus (IAV). MSGs include the disaccharide-repeating sulfated galactan from the red alga Botryocladia occidentalis, the tetrasaccharide-repeating sulfated fucans from the sea urchin Lytechinus variegatus and from the sea cucumber Isostichopus badionotus, and the two marine fucosylated chondroitin sulfates from the sea cucumbers I. badionotus and Pentacta pygmaea. Results indicate specificity of action against SARS-CoV-1 and SARS-CoV-2. Curiously, the MSGs showed decreased inhibitory potencies against MERS-CoV and negligible action against IAV. Among the five MSGs, the two sulfated fucans here studied deserve further attention since they have the lowest anticoagulant effects but still present potent and selective antiviral properties.

Polychrome labeling reveals skeletal triradiate and elongation dynamics and abnormalities in patterning cue-perturbed embryos

The larval skeleton of the sea urchin Lytechinus variegatus is an ideal model system for studying skeletal patterning; however, our understanding of the etiology of skeletal patterning in sea urchin larvae is limited due to the lack of approaches to live-image skeleton formation. Calcium-binding fluorochromes have been used to study the temporal dynamics of bone growth and healing. To date, only calcein green has been used in sea urchin larvae to fluorescently label the larval skeleton. Here, we optimize labeling protocols for two additional calcium-binding fluorochromes: xylenol orange and calcein blue- and demonstrate that these fluorochromes can be used individually or in nested pulse-chase experiments to understand the temporal dynamics of skeletogenesis and patterning. Using a pulse-chase approach, we show that the initiation of skeletogenesis begins around 15 ​h post fertilization. We also assess the timing of triradiate formation in embryos treated with a range of patterning perturbagens and demonstrate that triradiate formation is delayed and asynchronous in embryos ventralized via treatment with either nickel or chlorate. Finally, we measure the extent of fluorochrome incorporation in triple-labeled embryos to determine the elongation rate of numerous skeletal elements throughout early skeletal patterning and compare this to the rate of skeletal growth in embryos treated with axitinib to inhibit VEGFR. We find that skeletal elements elongate much more slowly in axitinib-treated embryos, and that axitinib treatment is sufficient to induce abnormal orientation of the triradiates.

Chemical Defense against Herbivory in the Brown Marine Macroalga Padina gymnospora Could Be Attributed to a New Hydrocarbon Compound.

Brown marine macroalga Padina gymnospora (Phaeophyceae, Ochrophyta) produces both secondary metabolites (phlorotannins) and precipitate calcium carbonate (CaCO3-aragonite) on its surface as potential defensive strategies against herbivory. Here, we have evaluated the effect of natural concentrations of organic extracts (dichloromethane-DI; ethyl acetate-EA and methanol-ME, and three isolated fractions) and mineralized tissues of P. gymnospora as chemical and physical resistance, respectively, against the sea urchin Lytechinus variegatus through experimental laboratory feeding bioassays. Fatty acids (FA), glycolipids (GLY), phlorotannins (PH) and hydrocarbons (HC) were also characterized and/or quantified in extracts and fractions from P. gymnospora using nuclear magnetic resonance (NMR) and gas chromatography (GC) coupled to mass spectrometry (CG/MS) or GC coupled to flame ionization detector (FID) and chemical analysis. Our results showed that chemicals from the EA extract of P. gymnospora were significantly important in reducing consumption by L. variegatus, but the CaCO3 did not act as a physical protection against consumption by this sea urchin. An enriched fraction containing 76% of the new hydrocarbon 5Z,8Z,11Z,14Z-heneicosatetraene exhibited a significant defensive property, while other chemicals found in minor amounts, such as GLY, PH, saturated and monounsaturated FAs and CaCO3 did not interfere with the susceptibility of P. gymnospora to L. variegatus consumption. We suggest that the unsaturation of the 5Z,8Z,11Z,14Z-heneicosatetraene from P. gymnospora is probably an important structural characteristic responsible for the defensive property verified against the sea urchin.

Variation in Trace Element Concentrations with Size in Sea Urchin Skeletal Components

Environmental ecotoxicology studies use sea urchin skeletal components to examine potential changes in concentrations of trace element pollutants. To date, such studies have not considered how body size may alter the innate concentrations of trace elements in a given skeletal component. The present study examines this issue using the common nearshore sea urchin Lytechinus variegatus as a model species. Concentrations of barium, boron, manganese, and strontium was measured using ICP-OES in three major skeletal subcomponents (Aristotle's lantern, spines, and test) of individuals' representative of five size cohorts spanning the late juvenile to adult. Concentrations of three of the four elements, barium, boron, and manganese, varied significantly with body size, largely declining as test diameter increased. As concentrations of trace elements was normalized to skeletal mass originated from collections of individuals in pristine bay waters and from the same population at the same time, differences in trace element concentrations are innately ontogenetic. Accordingly, future ecotoxicological studies employing skeletal subcomponents of sea urchins should consider the relationship between trace element concentration and body size when designing sampling regimes.

EchinoDB: an update to the web-based application for genomic and transcriptomic data on echinoderms

BackgroundHere we release a new version of EchinoDB, EchinoDB v2.0 (https://echinodb.uncc.edu). EchinoDB is a database of genomic and transcriptomic data on echinoderms. The initial database consisted of groups of 749,397 orthologous and paralogous transcripts arranged in orthoclusters by sequence similarity.ResultsThe updated version of EchinoDB includes two new major datasets: the RNA-Seq data of the brittle star Ophioderma brevispinum and the high-quality genomic assembly data of the green sea urchin Lytechinus variegatus. In addition, we enabled keyword searches for annotated data and installed an updated version of Sequenceserver to allow Basic Local Alignment Search Tool (BLAST) searches. The data are downloadable in FASTA format. The first version of EchinoDB appeared in 2016 and was implemented in GO on a local server. The new version has been updated using R Shiny to include new features and improvements in the application. Furthermore, EchinoDB now runs entirely in the cloud for increased reliability and scaling.ConclusionEchinoDB serves a user base drawn from the fields of phylogenetics, developmental biology, genomics, physiology, neurobiology, and regeneration. As use cases, we illustrate the function of EchinoDB in retrieving components of signaling pathways involved in the tissue regeneration process of different echinoderms, including the emerging model species Ophioderma brevispinum. Moreover, we use EchinoDB to shed light on the conservation of the molecular components involved in two echinoderm-specific phenomena: spicule matrix proteins involved in the formation of stereom endoskeleton and the tensilin protein that contributes to the capacity of the connective tissues to quickly change its mechanical properties. The genes involved in the former had been previously studied in echinoids, while gene sequences involved in the latter had been previously described in holothuroids. Specifically, we ask (a) if the biomineralization-related proteins previously reported only in sea urchins are also present in other, non-echinoid, echinoderms and (b) if tensilin, the protein responsible for the control of stiffness of the mutable collagenous tissue, previously described in sea cucumbers, is conserved across the phylum.

Effect of Diet and Body Size on Fecal Pellet Morphology in the Sea Urchin Lytechinus variegatus

Sea urchins are important inhabitants of many marine ecosystems. They are also an economic resource for many international fisheries and are an important animal model in developmental biology. Sea urchins ingest a variety of plant and animal matter, form a digesta pellet, and produce an egesta (fecal) pellet that contributes to benthic food webs. The size and morphology of fecal pellets produced by various size classes of the sea urchin Lytechinus variegatus fed a variety of natural, vegetable-based, or formulated diets were characterized in terms of two-dimensional area, length (long diameter), width (short diameter), and circularity, as well as by ultrastructure analysis using transmission electron microscopy (TEM). Sea urchins fed natural and vegetable-based ingredients produced a wide variety of irregularly shaped egesta that were loosely surrounded by mucus, whereas urchins fed a formulated diet produced highly circular egesta surrounded by a uniform multilayered mucus coat. Larger urchins (62 ± 3.1 g wet weight) consuming a formulated diet produced egesta that were larger in size than smaller adult (26.5 ± 2.7 g) or juvenile urchins (3.6 ± 0.4 g), with egesta length of 0.93 ± 0.06, 0.75 ± 0.05, and 0.61 ± 0.01 mm, respectively (mean ± 95% confidence interval [CI]; P < 0.001). Ultrastructural analysis of the egesta using TEM image analysis software from urchins consuming formulated diets indicated that pellets contained extensive bacterial communities, ranging from 31% to 69% of the total cross-sectional area of the pellet, with dense communities located near the surface of the pellet (9.51 × 108 cells/cm2). Qualitative examination of the egesta TEM suggested the presence of a diverse bacterial community. Following egestion, these microbial communities are suggested to have an important role in natural food webs, with potential value for integrated multitrophic aquaculture systems.

Effects of ocean acidification on the performance and interaction of fleshy macroalgae and a grazing sea urchin

When predicting the response of marine ecosystems to climate change, it is increasingly recognized that understanding the indirect effects of ocean acidification on trophic interactions is as important as studying direct effects on organism physiology. Furthermore, comprehensive studies that examine these effects simultaneously are needed to identify and link the underlying mechanisms driving changes in species interactions. Using an onshore ocean acidification simulator system, we investigated the direct and indirect effects of elevated seawater pCO2 on the physiology and trophic interaction of fleshy macroalgae and the grazing sea urchin Lytechinus variegatus. Macroalgal (Dictyota spp.) biomass increased despite decreased photosynthetic rates after two-week exposure to elevated pCO2. Algal tissue carbon content remained constant, suggesting the use of alternative carbon acquisition pathways beneficial to growth under acidification. Higher C:N ratios driven by a slight reduction in N content in algae exposed to elevated pCO2 suggest a decrease in nutritional content under acidification. Urchin (L. variegatus) respiration, biomass, and righting time did not change significantly after six-week exposure to elevated pCO2, indicating that physiological stress and changes in metabolism are not mechanisms through which the trophic interaction was impacted. Correspondingly, urchin consumption rates of untreated macroalgae (Caulerpa racemosa) were not significantly affected by pCO2. In contrast, exposure of urchins to elevated pCO2 significantly reduced the number of correct foraging choices for ambient macroalgae (Dictyota spp.), indicating impairment of urchin chemical sensing under acidification. However, exposure of algae to elevated pCO2 returned the number of correct foraging choices in similarly exposed urchins to ambient levels, suggesting alongside higher C:N ratios that algal nutritional content was altered in a way detectable by the urchins under acidification. These results highlight the importance of studying the indirect effects of acidification on trophic interactions simultaneously with direct effects on physiology. Together, these results suggest that changes to urchin chemical sensing and algal nutritional quality are the driving mechanisms behind surprisingly unaltered urchin foraging behavior for fleshy macroalgae under joint exposure to ocean acidification. Consistent foraging behavior and consumption rates suggest that the trophic interaction between L. variegatus and fleshy macroalgae may be sustained under future acidification. However, increases in fleshy macroalgal biomass driven by opportunistic carbon acquisition strategies have the potential to cause ecological change, depending on how grazer populations respond. Additional field research is needed to determine the outcome of these results over time and under a wider range of environmental conditions.

Levels of hydrocarbons and toxicity of water-soluble fractions of maritime fuels on neotropical invertebrates.

Accidents involving fuels and oil spills are among the main sources of hydrocarbons to the marine ecosystems and often damage the biota. Diesel and bunker oil are two examples of fuels with broad application that release hydrocarbons to the aquatic environment and little is known about their toxicity on tropical organisms. This study aimed to assess the toxicity of the water-soluble fraction (WSF) of diesel and bunker oils to neotropical marine invertebrates. Commercial fuels were purchased for WSF extraction, analyzed for total petroleum hydrocarbons (TPH), and polycyclic aromatic hydrocarbons (PAHs), and acute and chronic toxicity determined. The WSF analyzed contained varied levels of TPH and PAHs mixtures, especially low molecular weight PAHs; bunker WSF presented higher amounts of TPH and PAHs. Both WSFs tested produced significant mortality of the brine shrimp Artemia salina, affected the reproduction rate of the copepod Nitokra sp, and impaired the embryo-larval development of the mussel Perna perna and of the sea urchin Lytechinus variegatus. In general WSF from diesel was more toxic to the organisms tested, but bunker WSF was more toxic to embryos of L. variegatus. Toxicity started from concentrations of 3% WSF, which can be environmentally relevant after an oil spill, indicating that marine biota may be adversely affected in short term.

Colorless spherule cells and lysozyme contribute to innate immunological responses in the sea urchin Lytechinus variegatus, exposed to bacterial challenge

The sea urchin Lytechinus variegatus is considered a good candidate for aquaculture, but bacterial diseases are a major challenge in culture conditions. The innate immunological defenses of L. variegatus to bacterial challenges were assessed through hematology parameters, in vitro phagocytosis, lysozyme activity and total plasma protein concentrations in cell-free coelomic fluid. Adult sea urchins were inoculated with Microccocus lysodeikticus, Escherichia coli and Vibrio parahaemolyticus in the cavity coelomic. Filtrated and sterile seawater (FSW) injected and non-injected sea urchins were used as control groups. Righting time, external aspects and behavior of sea urchins were evaluated. Twenty-four hours post-inoculation, we found an increase in the population of colorless spherule cells (CLS), phagocytosis, and humoral responses in sea urchins challenged by bacterial inoculations. Righting time was not affected by the treatments and apparent external signs of disease were not observed at least during 96h post-inoculation. The immunological system of L. variegatus quickly eliminated pathogenic microorganisms. CLS and lysozyme activity cooperate in the immune defenses of L. variegatus, showing an extraordinary efficiency for adjusting the immune defenses under stress caused by microbes. We recommend that the cellular and humoral markers serve as routine tests to monitor health status in sea urchins.

The Gut Microbiota of Naturally Occurring and Laboratory Aquaculture Lytechinus variegatus Revealed Differences in the Community Composition, Taxonomic Co-Occurrence, and Predicted Functional Attributes

Sea urchins, in many instances, are collected from the wild, maintained in the laboratory aquaculture environment, and used as model animals for various scientific investigations. It has been increasingly evident that diet-driven dysbiosis of the gut microbiome could affect animal health and physiology, thereby impacting the outcome of the scientific studies. In this study, we compared the gut microbiome between naturally occurring (ENV) and formulated diet-fed laboratory aquaculture (LAB) sea urchin Lytechinus variegatus by amplicon sequencing of the V4 region of the 16S rRNA gene and bioinformatics tools. Overall, the ENV gut digesta had higher taxa richness with an abundance of Propionigenium, Photobacterium, Roseimarinus, and Flavobacteriales. In contrast, the LAB group revealed fewer taxa richness, but noticeable abundances of Arcobacter, Agarivorans, and Shewanella. However, Campylobacteraceae, primarily represented by Arcobacter spp., was commonly associated with the gut tissues of both ENV and LAB groups whereas the gut digesta had taxa from Gammaproteobacteria, particularly Vibrio spp. Similarly, the co-occurrence network displayed taxonomic organizations interconnected by Arcobacter and Vibrio as being the key taxa in gut tissues and gut digesta, respectively. Predicted functional analysis of the gut tissues microbiota of both ENV and LAB groups showed a higher trend in energy-related metabolisms, whereas amino acids, carbohydrate, and lipid metabolisms heightened in the gut digesta. This study provides an outlook of the laboratory-formulated diet-fed aquaculture L. variegatus gut microbiome and predicted metabolic profile as compared to the naturally occurring animals, which should be taken into consideration for consistency, reproducibility, and translatability of scientific studies.

Antiviral activities of four marine sulfated glycans against adenovirus and human cytomegalovirus

Broad-spectrum antivirals are more needed than ever to provide treatment options for novel emerging viruses and for viruses that lack therapeutic options or have developed resistance. A large number of viruses rely on charge-dependent non-specific interactions with heparan sulfate (HS), a highly sulfated glycosaminoglycan (GAG), for attachment to cell surfaces to initiate cell entry. As such, inhibitors targeting virion-HS interactions have potential to have broad-spectrum antiviral activity. Previous research has explored organic and inorganic small molecules, peptides, and GAG mimetics to disrupt virion-HS interactions. Here we report antiviral activities against both enveloped (the herpesvirus human cytomegalovirus) and non-enveloped (adenovirus) DNA viruses for four defined marine sulfated glycans: a sulfated galactan from the red alga Botryocladia occidentalis; a sulfated fucan from the sea urchin Lytechinus variegatus, and a sulfated fucan and a fucosylated chondroitin sulfate from the sea cucumber Isostichopus badionotus. As evidenced by gene expression, time of addition, and treatment/removal assays, all four novel glycans inhibited viral attachment and entry, most likely through interactions with virions. The sulfated fucans, which both lack anticoagulant activity, had similar antiviral profiles, suggesting that their activities are not only due to sulfation content or negative charge density but also due to other physicochemical factors such as the potential conformational shapes of these carbohydrates in solution and upon interaction with virion proteins. The structural and chemical properties of these marine sulfated glycans provide unique opportunities to explore relationships between glycan structure and their antiviral activities.

Patterns of Dispersion, Movement and Feeding of the Sea Urchin Lytechinus variegatus, and the Potential Implications for Grazing Impact on Live Seagrass

The sea urchin Lytechinus variegatus is a known grazer of both living and dead tissue of turtlegrass, Thalassia testudinum, occasionally denuding large areas of seagrass. Field studies have attempted to assess effects of herbivory on seagrass by enclosing urchins at various densities. However, it is unclear how unrestricted urchins affect seagrass at lower densities more typically observed in the field. This study describes movement, feeding, and distribution of L. variegatus within beds of T. testudinum in St. Joseph Bay, Florida (USA) to quantify this urchin’s impact as a seagrass grazer. Urchins were absent from portions of seagrass beds closest to shore, present at low densities midway across the bed, and at highest densities (up to ~5 individuals/m2) at the offshore edge of the bed. Urchins tended not to aggregate, moved twice as rapidly where seagrass cover was reduced, and moved &gt; 20X faster when placed in areas of open sand. Dead seagrass tissue occurred 4—30X more frequently on oral surfaces than living seagrass tissue. Fecal pellets with dead seagrass tissue were &gt; 3X more common than pellets with live seagrass tissue. Injury to seagrass leaves was more common along dead leaf sections than live sections (&gt; 2—10X). Overall, spatial distributions, movement, and diet indicate that L. variegatus at densities observed in this study would tend to have minimal effects on living seagrass. Episodic periods of denuding grassbeds reported in the literature suggest L. variegatus switches to live seagrass tissue as dead tissue becomes scarce during times of high urchin density.

Toposome: Source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816)

Sea urchins live in a challenging environment that requires rapid and efficient responses against pathogens and invaders. This response may be also important in reproductive processes once males and females release their gametes into water. In addition, the gonads are organs with dual function: reproductive organ and nutrient reserve, therefore it needs efficient protective mechanisms to preserve the nutrients as well as the reproductive cells. The aim of this study was to evaluate the presence and characterize antimicrobial molecules in the male and female gonads of the sea urchin Lytechinus variegatus. Through HPLC purification, antimicrobial activity test and mass spectrometry several antimicrobial molecules were found in the gonads of both gender. Computational in silico analyses showed that they are fragments of a glycoprotein called toposome, also known as major yolk protein (MYP) which is one of the major proteins found in the gonads. Although different functions have been reported for this protein, this is the first description of a direct antimicrobial activity in Lytechinus variegatus. The results indicate that when undergoing proteolysis the toposome generates different fragments with antimicrobial activity which may indicate the importance of a rapid defense response strategy against invading microorganisms in the gonads used by both males and females sea urchins.

Shift in bacterial taxa precedes morphological plasticity in a larval echinoid

Morphological plasticity is an adaptive response to heterogenous environments when a fitness advantage is conferred. Larval sea urchins, for example, are hypothesized to increase individual fitness in dilute feeding environments by elongating their feeding structure relative to their body size. Morphological plasticity for larval sea urchins is also coupled with significant shifts in the associated bacterial community, but whether this change to the associated microbiota occurs before, during, or following the expression of morphological plasticity is unclear. Using the sea urchin Lytechinus variegatus, we compare the temporal pattern of the microbial community and how it relates to the timing of morphological plasticity for larvae cultured in different food concentrations. From prefeeding through the expression of morphological plasticity, we observed that the microbiota associated with L. variegatus larvae exhibit a four-stage successional pattern, where changes in this community shift prior to the expression of the environment-specific morphology. The high food treatment, which results in the short-arm phenotype, deviated from the microbial trajectory of larval siblings prior to measurable morphological plasticity. These data suggest that a holobiont may exhibit shifts in the associated bacterial community corresponding with variation in the feeding environment that could occur in anticipation of or prior to morphological plasticity.

Quantitative analysis of pellets on beaches of the São Paulo coast and associated non-ingested ecotoxicological effects on marine organisms

We evaluated the densities of plastic pellets on beaches along the coast of São Paulo State in Brazil in order to establish a relationship between their spatial distribution and their distance from the main source of pellets. We hypothesized that pellets would be concentrated close to their main source (in this case, the Port of Santos). We also tested whether beached pellets could induce toxicity in water and sediment samples, as well as whether pellet color, sample site (beach), and pellet density were correlated with toxicity. We observed a decreasing gradient of pellet density along the São Paulo coast, with higher densities detected within Santos Bay, which is close to the Port of Santos. Beached pellets induced water-based toxicity in the sea urchin Lytechinus variegatus and the copepod Nitocra sp. at high densities but were not capable of producing toxicity at environmentally realistic densities.