According to previous studies, the Lixiahe area in the east of the Jianghuai River was an alternate environment of land and sea in the middle Holocene, and it was not until the late Holocene that the eastern Jianghuai completely became a terrestrial environment. However, recent archaeological studies have found that the extensive Neolithic sites in the Lixiahe area have recorded the rich human activities and cultural connotations of the prehistoric civilization in the Middle Holocene. In this paper, the Gangxi section of Jianhu Lake, Jiangsu Province (GX2) was selected and pollen analysis was fulfilled, then the geomorphic evolution process of the study area from sea to land was investigated according to the palynological assemblages and algae fossils of brackish water, semi-saline water, fresh water and terrestrial in the section strata. During the period of 8500–3800 cal. BP, GX2 was affected by multiple factors such as sea surface fluctuation, ocean flow and sediment deposition carried by seagoing rivers. Since 5800 cal. BP, the area east of the Grand Canal between the Yangtze River and the Huaihe River, and the west of the Yangzhai town, Funing–Longgang town, Yancheng–Dagang town and Yancheng–west of the Dongtai–Hai’an line, have become a terrestrial environment. After the eastern Jianghuai became a land, the Liangzhu culture (5300–4300 cal. BP) in the Taihu Lake basin in the south wing of the Yangtze River delta expanded to the eastern Jianghuai area, and the Longshan culture and Yueshi culture in the Haidai area in the north also migrated south to the eastern Jianghuai area. Admittedly, the main reason in the process of Neolithic cultural development is the internal motivation that the ancient ancestors struggled with nature and pushed forward the continuous development of civilizations. However, our study explains the palaeogeographical origin of Neolithic culture in the eastern and coastal areas of Jianghuai in the middle Holocene, and meanwhile, provides an example for the man–land relationship research on Neolithic culture.
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