Sea Fan Research Articles

Sea fans and their associated fauna in the marine animal forests of Burdwood Bank at 54°S, SW Atlantic Ocean

Sea fans and their associated fauna in the marine animal forests of Burdwood Bank at 54°S, SW Atlantic Ocean

Constraining the origin of the Norwegian strandflat – The influence of isostatic and dynamic surface changes

The Norwegian strandflat is a prominent low-relief bedrock surface found near sea level along most of the west coast of Norway. Its origin has been discussed throughout the last 130 years but is yet to be resolved. Some studies suggest that the strandflat represent a tropical weathering front of Mesozoic age that has since been buried and re-exhumed, while others relate its origin to Pleistocene periglacial and glacial processes and/or wave-induced weathering and erosion. Previous interpretations of the strandflat have considered postglacial isostatic uplift, but the impacts of isostatic changes due to glacial erosion and deposition, as well as dynamic surface changes driven by mantle convection, have been largely overlooked. Here we examine how geomorphological-driven isostatic changes and dynamic surface changes have influenced the land-surface elevation along the Norwegian coast during late Pliocene-Quaternary (the last ca. 3 million years). We employ quantitative estimates of glacial erosion and deposition to assess the flexural isostatic response from the resulting load changes. Our analyses show that patterns of geomorphic isostatic adjustments and dynamic surface changes are generally not reflected in the present elevation of the strandflat. Only the loading effect from the deposition of the North Sea Fan can clearly be correlated with the submerged strandflat found near Stad (~62 °N). Our results imply that if the strandflat formed synchronously along the Norwegian coast as a flat surface at sea level, the strandflat we observe today must have developed after the majority of late Pliocene-Quaternary glacial erosion took place, but prior to the main deposition of the North Sea Fan. This would place strandflat formation within the last few glacial cycles, but before the Last Glacial Maximum (LGM). This inferred pre-LGM age of the strandflat is generally consistent with cosmogenic nuclide exposure ages and observed striations on the strandflat. Finally, we examine ice cover and land-surface changes relative to sea level during the last 80,000 years and find no extended periods favorable for synchronous strandflat formation across all regions along the Norwegian coast. This implies that either the strandflat is diachronous, or that the processes of formation have either been extremely fast under certain conditions or are independent of sea level, for instance related to glacial erosion.

The gas hydrate system of the western Black Sea Basin

The basin-scale distribution of gas hydrates and methane migration pathways in the western Black Sea remains enigmatic, owing to the region's complex geological history. Characterizing the abundant gas hydrate accumulations across temporal scales poses significant challenges. In this study, we developed and applied a 3D large-scale numerical model to study the formation, development, and fate of natural gas hydrate systems in the western Black Sea sub-basin. Our model enables us to simulate the dynamic evolution of basin geometry and facies distribution over the last 98 million years and under dynamically changing boundary conditions, e.g. due to sea-level changes. Our study estimates the total volume of gas hydrates stored within western Black Sea sediments at ∼14,607 MtC, equivalent to ∼30,073 × 1011 m3 of CH4 (at STP conditions) which is about 5 times higher than average gas hydrate density at continental margins. Our findings reveal three distinct mechanisms driving gas hydrate reservoir formation within the reconstructed sub-basin: gas hydrate recycling zones, chimney-like structure formation, and gas hydrate deposits associated with paleo-deep sea fans. We identified and simulated key controlling parameters, related to methane migration pathways and regional geomorphology, governing each type of hydrate formation. Subsequently, we conduct a detailed analysis of regional gas hydrate systems, focusing on the Dniepr paleo-fan system, the Danube paleo-fan region, multiple offshore locations near Turkey, and the central Black Sea area. Furthermore, we investigate various biogenic methane formation kinetics and their impact on basin-scale methane generation. Our sensitivity studies enable us to predict the optimal temperature range for microbial activity driving methanogenesis in the Black Sea sediments at 30 °C–40 °C. Overall, our study provides a novel understanding and quantitative correlation between methane generation, migration, and storage in the form of gas hydrates on a basin-scale in the western Black Sea.

Sedimentation record of a complete glacial-interglacial cycle: Down-slope deposits, contourites and plumites of the North Sea trough mouth fan

Trough mouth fans (TMFs) are major submarine depocentres that form in front of ice streams advancing onto the continental shelf, and they provide extensive records of past glaciations and ice-sheet dynamics. However, early research about TMFs was conducted using primarily 2D seismic data, which limits the insights on the deposit's geometries and internal structures and more recent studies using 3D data have focused on the general depositional history of TMFs. Here, we assess the spatial and temporal changes of the glacio-marine landforms and deposits throughout the last glacial-interglacial cycle (Marine Isotope Stage (MIS) 6 to 1, ∼130 ka to present) recorded in a >550 m thick progradational sequence. We use 3D reflection seismic data with significant vertical resolution (up to 2 m) imaging over 14000 km2 from the uppermost North Sea Fan (NSF) to document the seismic stratigraphy and sedimentary processes, by combining horizon picking and geomorphological analyses. The studied interval comprises five mappable units, four on the slope and one on the shelf, spanning from the modern seabed down to the top of the Tampen Slide (∼130 ka). Five different channel types (here included slide scars), as well as contourite and plumite packages are described based on their seismic facies, morphology, geometry, and stratigraphical position. We propose that contour currents are responsible for sediment delivery after the Tampen Slide deposition during the interglacial period before the onset of the Last Glacial Maximum, when high meltwater influx from the shelf produced turbidites. Sedimentation became debris-flow-dominated towards the end of the period of shelf-edge glaciation. The deglacial interval is characterized by the deposition of plumites on the slope. On the shelf, subglacial tills are deposited during both glacial and deglacial periods. We calculate an average sedimentation rate of <1 m/kyr during the interglacial (MIS 5-3), and around 50–65 m/kyr during the glacial and deglacial periods (MIS2-early MIS1). Thus, we document a change of two orders of magnitude in sedimentation rate during a glacial cycle on the slope of a glaciated margin.

Tracing Geographic and Molecular Footprints of Copepod Crustaceans Causing Multifocal Purple Spots Syndrome in the Caribbean Sea Fan Gorgonia ventalina

The recent rise in ocean temperatures, accompanied by other environmental changes, has notably increased the occurrence and spread of diseases in Octocorallia, many species of which are integral to shallow tropical and subtropical coral reef ecosystems. This study focuses on the understanding of these diseases, which has been largely limited to symptomatic descriptions, with clear etiological factors identified in only a fraction of cases. A key example is the multifocal purple spots syndrome (MFPS) affecting the common Caribbean octocoral sea fan Gorgonia ventalina, linked to the gall-forming copepods of the genus Sphaerippe, a member of the widespread family, Lamippidae. The specialized nature of these copepods as endoparasites in octocorals suggests the potential for the discovery of similar diseases across this host spectrum. Our investigation employed four molecular markers to study disease hotspots in Saint Eustatius, Curaçao, northwest and southwest Cuba, and Bonaire. This led to the discovery of a group of copepod species in these varied Caribbean locations. Importantly, these species are morphologically indistinguishable through traditional methods, challenging established taxonomic approaches. The observed diversity of symbionts, despite the host species’ genetic uniformity, is likely due to variations in larval dispersal mechanisms. Our phylogenetic analyses confirmed that the Lamippidae copepods belong to the order, Poecilostomatoida (Copepoda), and revealed their sister group relationship with the Anchimolgidae, Rhynchomolgidae, and Xarifiidae clades, known for their symbiotic relationships with scleractinian corals. These results add to our understanding of the evolutionary and ecological interactions of copepods and their hosts, and the diseases that they cause, and are important data in a changing climate.

Salt-related gravity-driven processes in the Levant Basin, Eastern Mediterranean: Insights from physical modeling

The characterization of salt tectonics and its gravity-driven deformation processes are the key to a better understanding of the structural evolution of salt-bearing rifted margins. Unlike most salt basins that have experienced long-lasting deformation, the Messinian evaporites in the Levant Basin have been moderately deformed, offering the opportunity to study the early stage of salt tectonic deformation. Despite the availability of seismic reflection, borehole and bathymetrical data, some uncertainties still exist about the mechanisms responsible for the deformation and structural features observed in the deep-water Levant Basin. Our study includes physical experiments based on published seismic and structural interpretations conducted in the Levant Basin. Our physical experiments take into consideration the main driving parameters that controlled the development of the deep-water Levant Basin, testing the interplay and impact of gravity gliding and spreading from the Levant Margin, gravity spreading from the Nile Deep Sea Fan, and the influence of the passive buttress of the Eratosthenes Seamount. Deformation was imposed by depositing successive sand lobes and/or by tilting the experimental table. The physical models included a thick viscous silicone layer, analogue of the Messinian evaporitic sequence, overlain by a granular overburden, simulating the brittle clastic post-Messinian succession. Results show that the prominent gravity-driven force affecting the deformation pattern of the deep-water Levant Basin is the gravity spreading from the Nile Deep Sea Fan, whereas gravity spreading and gliding from the Levant Margin affect only the proximal to the margin areas. Additionally, the buttressing effect of the Eratosthenes Seamount and the location of the salt basin pinch-out played an important role in the final deformation pattern of this region of the Eastern Mediterranean.

Rare, long-distance dispersal underpins genetic connectivity in the pink sea fan, Eunicella verrucosa.

Characterizing patterns of genetic connectivity in marine species is of critical importance given the anthropogenic pressures placed on the marine environment. For sessile species, population connectivity can be shaped by many processes, such as pelagic larval duration, oceanographic boundaries and currents. This study combines restriction-site associated DNA sequencing (RADseq) and passive particle dispersal modelling to delineate patterns of population connectivity in the pink sea fan, Eunicella verrucosa, a temperate octocoral. Individuals were sampled from 20 sites covering most of the species' northeast Atlantic range, and a site in the northwest Mediterranean Sea to inform on connectivity across the Atlantic-Mediterranean transition. Using 7510 neutral SNPs, a geographic cline of genetic clusters was detected, partitioning into Ireland, Britain, France, Spain (Atlantic), and Portugal and Spain (Mediterranean). Evidence of significant inbreeding was detected at all sites, a finding not detected in a previous study of this species based on microsatellite loci. Genetic connectivity was characterized by an isolation by distance pattern (IBD) (r 2 = 0.78, p < 0.001), which persisted across the Mediterranean-Atlantic boundary. In contrast, exploration of ancestral population assignment using the program ADMIXTURE indicated genetic partitioning across the Bay of Biscay, which we suggest represents a natural break in the species' range, possibly linked to a lack of suitable habitat. As the pelagic larval duration (PLD) is unknown, passive particle dispersal simulations were run for 14 and 21 days. For both modelled PLDs, inter-annual variations in particle trajectories suggested that in a long-lived, sessile species, range-wide IBD is driven by rare, longer dispersal events that act to maintain gene flow. These results suggest that oceanographic patterns may facilitate range-wide stepping-stone genetic connectivity in E. verrucosa and highlight that both oceanography and natural breaks in a species' range should be considered in the designation of ecologically coherent MPA networks.

A Legal Analysis of Cyber-Enabled Wildlife Offences in India: A Qualitative Case Study of Sea Fans (Gorgonia spp.) on YouTube

With the advent of the Internet, offences against threatened species have transitioned online. Such species are directly or indirectly traded on social media despite being protected under Indian wildlife law. A qualitative case study was undertaken to assess the preparedness of national law and policy in prohibiting such offences. Sixty-three YouTube links on sea fans in the Hindi language were accessed over 8 weeks, and the information generated by both content creators and audiences was gathered and categorized for analysis. The legal provisions were then interpreted and applied to assess the extent to which the parties involved could be held liable. Our investigation shows that of these video links, the content creators directly offered specimens for sale in 15.87% of instances, demonstrated physical possession of wild specimens in 23.81% of these posts, and were involved in both activities in 20.63% of the links, which in our analysis is explicitly prohibited under national law. The remaining 39.68% of video links merely disseminated information on the relevance or usage of species in occult or religious practices, for which no express legal provision currently exists. Certain indirect legal provisions were found to be relevant; however, there were challenges associated with their implementation. Even the liability of a social media company was found to be limited if it can be demonstrated that the company exercised due diligence. Therefore, there is a need to explicitly regulate online content that has the potential to drive an unlawful demand for protected species alongside the imposition of enhanced liability on social media companies. Such measures, coupled with community awareness, can reduce cyber-enabled wildlife offences committed through social media channels.

Microbial shifts associated to ENSO-derived thermal anomalies reveal coral acclimation at holobiont level

The coral microbiome conforms a proxy to study effects of changing environmental conditions. However, scarce information exists regarding microbiome dynamics and host acclimation in response to environmental changes associated to global-scale disturbances. We assessed El Niño Southern Oscillation (ENSO)-derived thermal anomalies shifts in the bacterial microbiome of Pacifigorgia cairnsi (Gorgoniidae: Octocorallia) from the remote island of Malpelo in the Tropical Eastern Pacific. Malpelo is a hot spot of biodiversity and lacks direct coastal anthropogenic impacts. We evaluated the community composition and predicted functional profiles of the microbiome during 2015, 2017 and 2018, including different phases of ENSO cycle. The bacterial community diversity and composition between the warming and cooling phase were similar, but differed from the neutral phase. Relative abundances of different microbiome core members such as Endozoicomonas and Mycoplasma mainly drove these differences. An acclimated coral holobiont is suggested not just to warm but also to cold stress by embracing similar microbiome shifts and functional redundancy that allow maintaining coral’s viability under thermal stress. Responses of the microbiome of unperturbed sea fans such as P. cairnsi in Malpelo could be acting as an extended phenotype facilitating the acclimation at the holobiont level.

Diversity of Nigrospora (Xylariales, Apiosporaceae) Species Identified in Korean Macroalgae Including Five Unrecorded Species

Nigrospora (Xylariales, Apiosporaceae) consists of species of terrestrial plant endophytes and pathogens. Nigrospora has also been reported in marine environments such as mangroves, sea fans, and macroalgae. However, limited research has been conducted on Nigrospora associated with macroalgae. Here, we isolated Nigrospora species from three types of algae (brown, green, and red algae) from Korean islands (Chuja, Jeju, and Ulleung) based on phylogenetic analyses of multigenetic markers: the internal transcribed spacers (ITS), beta-tubulin (BenA), and translation elongation factor 1 (TEF1-α). A total of 17 Nigrospora strains were isolated from macroalgae and identified as nine distinct species. The majority of Nigrospora species (seven) were found on brown algae, followed by red algae (three), and then green algae (two). To our understanding, this study represents the first account of N. cooperae, N. covidalis, N. guilinensis, N. lacticolonia, N. osmanthi, N. pyriformis, and N. rubi occurring in marine environments. Additionally, this study provides the first report of the occurrence of N. cooperae, N. covidalis, N. guilinensis, N. lacticolonia, and N. osmanthi in South Korea. This study will provide valuable insights for future research exploring the functions of fungi in macroalgal communities.

Using regional‐scale predictive habitat models to assess protection and identify potential locations for additional management or monitoring for a species of conservation interest

Abstract Marine protected areas (MPAs) and associated management measures are being implemented to conserve marine benthic species. To make effective decisions, marine managers need data on the distributions of species of interest and anthropogenic pressures, but also the potential connectivity between habitat patches. To explore how predictive modelling can be utilized in such a process, a model was developed to predict the distribution of northern sea fan, Swiftia pallida, habitat around parts of Scotland, Northern Ireland, and Ireland. The predicted distribution of suitable habitat was compared with spatial data on mobile bottom‐contacting fishing activity, and the location of MPAs designated for benthic features, and the management measures within MPAs that restrict mobile bottom‐contacting fishing activity, which is the main pressure‐causing activity that S. pallida are sensitive to. Over 20% of predicted suitable habitat is within MPAs, over 10% is within MPA management measures and over 48% is within areas that have experienced no bottom contacting‐fishing activity, which is significantly higher than the equivalent values for the study region as a whole. However, patches were identified that potentially experience above average levels of fishing activity and remain unmanaged, including within MPAs designated for associated features. The analysis also highlighted patches that could be candidates for monitoring recovery and the locations of unknown populations. For each patch of suitable habitat, the number of other patches within 22 km, a previously published estimate of dispersal distance for S. pallida, was used as a proxy for connectedness. Connectedness was estimated to be greatest for patches towards the centre and west of the study region. The results indicate how the outputs of predictive distribution models can be used in conjunction with other data to prioritize areas for surveys and identify locations where effective management may facilitate conservation or the recovery of benthic species.

Secondary Metabolites, Biological Activities, and Industrial and Biotechnological Importance of Aspergillus sydowii.

Marine-derived fungi are renowned as a source of astonishingly significant and synthetically appealing metabolites that are proven as new lead chemicals for chemical, pharmaceutical, and agricultural fields. Aspergillus sydowii is a saprotrophic, ubiquitous, and halophilic fungus that is commonly found in different marine ecosystems. This fungus can cause aspergillosis in sea fan corals leading to sea fan mortality with subsequent changes in coral community structure. Interestingly, A. sydowi is a prolific source of distinct and structurally varied metabolites such as alkaloids, xanthones, terpenes, anthraquinones, sterols, diphenyl ethers, pyrones, cyclopentenones, and polyketides with a range of bioactivities. A. sydowii has capacity to produce various enzymes with marked industrial and biotechnological potential, including α-amylases, lipases, xylanases, cellulases, keratinases, and tannases. Also, this fungus has the capacity for bioremediation as well as the biocatalysis of various chemical reactions. The current work aimed at focusing on the bright side of this fungus. In this review, published studies on isolated metabolites from A. sydowii, including their structures, biological functions, and biosynthesis, as well as the biotechnological and industrial significance of this fungus, were highlighted. More than 245 compounds were described in the current review with 134 references published within the period from 1975 to June 2023.

Three new species of the sea fan genus Muricea (Cnidaria, Octocorallia, Plexauridae) from the northwest region of Mexico.

Twenty-one nominal species of Muricea have been reported in the Eastern Pacific with nine of them reported in the Mexican Pacific. We describe three new species of Muricea: Muriceaambaraesp. nov. and Muriceacacaosp. nov., from rocky reefs on the central and the northern Gulf of California and the Pacific coast of Baja California Sur, and Muriceamolinaisp. nov., from the Pacific coast of Baja California Sur. Muriceaambaraesp. nov. and M.cacaosp. nov. are taxonomically allied to the nominal species Muriceafruticosa Verrill, 1869 due to the morphological similarity of colony growth patterns and the phylogenetic closeness based on the mitochondrial MutS gene (mtMutS); but differ mainly in the calyx form and composition of sclerites. The main morphological differences between the new Muricea species are in their sclerite forms and color; M.ambaraesp. nov. has orange-colored colonies, thin leaf spindles and tuberculated blunt spindles, while M.cacaosp. nov. has dark brown colored colonies, strong spinous spindles and an absence of tuberculated blunt spindles. Muriceamolinaisp. nov. is phylogenetically close and morphologically similar to Muriceasquarrosa Verrill, 1869 in the growth form of the colony and tubular calyces; but has dark brown colored colonies and has calyces from the base to the branch tips. With these three new species, the total number of Muricea species reported in the Mexican northwest region increases to twelve and a total of 24 nominal species in the Eastern Pacific.

A combined diagnostic approach for the investigation of lesions resembling aspergillosis in Caribbean sea fans (Gorgonia spp.).

Aspergillosis of gorgonian sea fans is a Caribbean-wide disease characterized by focal, annular purple pigmentation with central tissue loss. We applied a holistic diagnostic approach including histopathology and a combination of culture and direct molecular identification of fungi to evaluate these lesions with the goal of determining the diversity of associated micro-organisms and pathology. Biopsies were collected from 14 sea fans without gross lesions and 44 sea fans with lesions grossly consistent with aspergillosis in shallow fringing reefs of St. Kitts. Histologically, the tissue loss margin had exposure of the axis and amoebocyte encapsulation with abundant mixed micro-organisms. Polyp loss, gastrodermal necrosis, and coenenchymal amoebocytosis were at the lesion interface (purpled area transitioning to grossly normal tissue) with algae (n = 21), fungus-like hyphae (n = 20), ciliate protists (n = 16), cyanobacteria (n = 15), labyrinthulomycetes (n = 5), or no micro-organisms (n = 8). Slender, septate hyaline hyphae predominated over other morphological categories, but were confined to the axis with little host response other than periaxial melanization. Hyphae were absent in 6 lesioned sea fans and present in 5 control biopsies, questioning their pathogenicity and necessary role in lesion causation. From cultivation, different fungi were isolated and identified by sequencing of the nuclear ribosomal internal transcribed spacer region. In addition, 2 primer pairs were used in a nested format to increase the sensitivity for direct amplification and identification of fungi from lesions, thereby circumventing cultivation. Results suggest mixed and opportunistic infections in sea fans with these lesions, requiring longitudinal or experimental studies to better determine the pathogenesis.

The First Genome of the Cold-Water Octocoral, the Pink Sea Fan, Eunicella verrucosa.

Cold-water corals form an important part of temperate benthic ecosystems by increasing three-dimensionality and providing an important ecological substrate for other benthic fauna. However, the fragile three-dimensional structure and life-history characteristics of cold-water corals can leave populations vulnerable to anthropogenic disturbance. Meanwhile, the ability of temperate octocorals, particularly shallow-water species, to respond to adjustments in their environment linked to climate change has not been studied. This study reports the first genome assembly of the pink sea fan (Eunicella verrucosa), a temperate shallow-water octocoral species. We produced an assembly of 467 Mb, comprising 4,277 contigs and an N50 of 250,417 bp. In total, 213 Mb (45.96% of the genome) comprised repetitive sequences. Annotation of the genome using RNA-seq data derived from polyp tissue and gorgonin skeleton resulted in 36,099 protein-coding genes after 90% similarity clustering, capturing 92.2% of the complete Benchmarking Universal Single-Copy Orthologs (BUSCO) ortholog benchmark genes. Functional annotation of the proteome using orthology inference identified 25,419 annotated genes. This genome adds to the very few genomic resources currently available in the octocoral community and represents a key step in allowing scientists to investigate the genomic and transcriptomic responses of octocorals to climate change.

Conservation status of upper-mesophotic octocoral habitats at Sporades Archipelago (Aegean Sea)

Octocoral forests created by the yellow sea fan Eunicella cavolini and the red sea fan Paramuricea clavata were studied at the National Marine Park of Alonissos Northern Sporades (Aegean Sea, Greece), between 30 and 45 m depth, in order to assess their conservation status and the occurrence of both natural and anthropogenic stressors. The area was characterized by rich and dense coral forests, with densities up to 55.2 colonies m−2 for E. cavolini and 28.0 colonies m−2 for P. clavata. The coral population showed signs of stress, although mortality was low. A combination of stressors linked to global warming and fishing impacts, including macroalgal epibiosis, tip necrosis, increasing coral feeders, and abandoned fishing gears, could impair the status of these habitats in the near future. Although the effects of climate change are global, local conservation actions may reduce direct anthropogenic impacts and enhance habitats' resilience.

Multiscale flow between the branches and polyps of gorgonians.

Gorgonians, including sea fans, are soft corals well known for their elaborate branching structure and how they sway in the ocean. This branching structure can modify environmental flows to be beneficial for feeding in a particular range of velocities and, presumably, for a particular size of prey. As water moves through the elaborate branches, it is slowed, and recirculation zones can form downstream of the colony. At the smaller scale, individual polyps that emerge from the branches expand their tentacles, further slowing the flow. At the smallest scale, the tentacles are covered in tiny pinnules where exchange occurs. In this paper, we quantified the gap to diameter ratios for various gorgonians at the scale of the branches, the polyp tentacles and the pinnules. We then used computational fluid dynamics to determine the flow patterns at all three levels of branching. We quantified the leakiness between the branches, tentacles and pinnules over the biologically relevant range of Reynolds numbers and gap-to-diameter ratios, and found that the branches and tentacles can act as either leaky rakes or solid plates depending upon these dimensionless parameters. The pinnules, in contrast, mostly impede the flow. Using an agent-based modeling framework, we quantified plankton capture as a function of the gap-to-diameter ratio of the branches and the Reynolds number. We found that the capture rate depends critically on both morphology and Reynolds number. The results of the study have implications for how gorgonians modify ambient flows for efficient feeding and exchange.

Hurricane Kay (September 2022) Impacts Rocky Shores in the Loreto Area of Baja California Sur, Mexico

This project follows a tradition of survey work undertaken to appraise physical and biological damage in the aftermath of hurricane-strength winds and waves at a given locality where conditions were well documented prior to the arrival of a particular storm. The locality is the 12 m limestone terrace at Arroyo Blanco on the eastern shores of Isla del Carmen in Baja California Sur, Mexico. A study undertaken in February 2018 established that the surface of the terrace is covered by a coastal boulder deposit that features large slabs of limestone pealed from the outer edge by strong surf attributed to storms of hurricane intensity but unknown date. The largest slabs tend to be rectilinear in shape vulnerable to dislodgement along horizonal bedding planes and weaknesses in vertical joints. These blocks are sufficiently large and weigh enough that movement by humans without necessary mechanical equipment would be impossible. Hurricane Kay, rated as a Category 2 storm, struck the island on 8 September 2022 and an effort was made to visit the area for reconnaissance and detailed survey work soon afterwards. Although a Category 2 storm lacked the energy to remobilize the largest limestone slabs on the terrace, it was found that the storm was sufficient to disturb the adjacent seabed and redeposit as many as 44 sea fans onto the terrace by overwash; the sea fans belonged to the species Pacifigorgia adamsi. Moreover, a species of land plant common to the limestone terrace is the Gulf Star Violet (Stenotis mucronate); it suffered significant desiccation and death due to saltwater exposure. The occurrence of large potholes on the limestone terrace represents a style of physical erosion previously undocumented at the locality and rarely seen elsewhere on rocky shores.

Leptogorgia sarmentosa (Anthozoa: Octocorallia) in NE Sardinia (Mediterranean Sea): distribution and growth patterns

Distribution and occurrence data of the sea fan Leptogorgia sarmentosa (Esper, 1791) in the Mediterranean Sea were updated thanks to new photographic surveys, highlighting the presence of the species off NE Sardinia and, in particular, in the Tavolara-Punta Coda Cavallo Marine Protected Area (TPCCMPA). The estimated average distance among colonies strongly suggests that they are not able to sexually reproduce and that they probably can be considered a sterile pseudo-population supported by inputs of larvae from the Italian mainland coast towards Sardinia. The putative conveyor belt for larval input to this area is a small permanent gyre (the North-Tyrrhenian Gyre) counterclockwise moving from the Italian to the Sardinian coasts. In TPCCMPA, the growth of one colony settled at 30 m depth was investigated across 7 years (2016–2022). The morphometric parameters indicated a linear development of the colony, in terms of branch development and 3D complexity estimated through the fractal dimension. The observed growth rates were not regular but were reached through a highly variable positive and negative oscillation. A significant correlation was obtained between growth rate and average Autumn and Winter water temperature, suggesting that somatic growth occurs in these periods. In the context of long-lasting elevated seawater temperatures and local damage linked to fishing activities, L. sarmentosa was confirmed to be a resilient species compared with other erect seascape components, like other gorgonians and sponges.

Optical coherence tomography angiography patterns of type 1 macular neovascularization in age-related macular degeneration patients.

To compare morphologic characteristics of type 1 macular neovascularization (MNV) flow pattern in treatment-naïve and previously treated patients with age-related macular degeneration (AMD) as assessed by optical coherence tomography angiography (OCTA). Cross-sectional study. Macular OCT angiography images were acquired using RTVue XR Avanti with AngioVue. Distinct morphologic biomarkers and quantifiable features of the neovascular membranes were studied on en-face projection images comparing treatment-naïve and previously treated patients. The study included 68 eyes of 58 patients. Among them, 24 eyes were treatment-naïve, and the remaining eyes had received a mean of 19.6 injections. Immature lesions were more associated with treatment-naïve eyes and hyper-mature lesions were associated with previously treated eyes (p = 0.005). Tangle pattern was associated with treatment-naïve eyes (p = 0.013), whereas mature core vessels and sea fan pattern were associated more with previously treated eyes (p = 0.001 and p = 0.044, respectively). Vascular density of the neovascular membrane was higher in the treatment-naïve group (p = 0.036) and the average MNV area was similar between the 2 groups (p = 0.683). Based on OCTA, morphologic biomarkers of type 1 MNV might be an indication of previous treatment. The MNV pattern can improve our understanding of its maturation under anti-VEGF treatment and might be valuable to better guide therapeutic decisions and provide more personalized care to patients with AMD.