SEA Domain Research Articles

Scientific Diplomacy: An Opportunity for Security and National Defense in Colombia

The present paper introduces the concept of Science Diplomacy for Security and Defense in Colombia as a means to pursue Soft Power activities that can contribute to both the perception of security by the community, and the development of Defense strategies for the country. For this purpose, the document begins by making a review of the definition of Scientific Diplomacy for Security and National the Defense and continues into highlighting emblematic cases from Colombia, as the central argument into recognizing the great potential this area has for Security and National Defense in the domains of land, sea, air and cyberspace. The scope of the research is descriptive, as its intention is to be a starting point for further analysis on the subject. The problem identified is the lack of recognition of the potential that science diplomacy represents both in terms of competitive advantage for geopolitics and as a contribution to the image of the military forces as an organization whose functions go beyond purely war activities to include important scientific developments. The paper concludes by issuing general recommendations to achieve such recognition.

The People’s Republic of China’s Strategy in The Three Sea Domain: The East China Sea, South China Sea, Indian Ocean, and The US’s Struggle for Presence in This Region

The People’s Republic of China’s Strategy in The Three Sea Domain: The East China Sea, South China Sea, Indian Ocean, and The US’s Struggle for Presence in This Region

Research on Aircraft Engines and Advanced Power in Land, Sea and Air

This paper provides a comprehensive overview of advanced propulsion technologies in military applications across land, sea, and air domains. It examines the theoretical foundations of propulsion systems, tracing the evolution of aircraft engines from early piston designs to modern jet propulsion. The study explores current state-of-the-art technologies, including advanced turbofan engines, hybrid electric systems, and nuclear propulsion for naval vessels. Special attention is given to emerging fields such as unmanned aerial vehicle propulsion, hypersonic technologies, and stealth engine designs. The research also delves into futuristic concepts like magnetohydrodynamic propulsion and pulse detonation engines. Throughout the analysis, common themes of improved efficiency, enhanced power-to-weight ratios, and increased operational capabilities are highlighted. This paper aims to provide a holistic view of the current landscape and future trajectory of military propulsion technologies, underlining their critical role in shaping modern warfare capabilities.

Culture: a sixth domain and the introduction of the ‘C6ISRT’ framework

ABSTRACT The traditional domains of land, sea, and air warfare have long held prominence, while space and cyberspace have recently gained recognition as spheres of conflict. As conflicts continue to evolve, the domains of war must expand to accommodate new dimensions of engagement and integrate them alongside those human-centred factors that have previously held considerable sway. It is imperative, therefore, to recognise culture as the sixth domain and reflect upon its profound influence on group thinking, decision-making, and behaviour. Integrating culture into the Command, Control, Communications, Computers, Cyber, Intelligence, Surveillance, Reconnaissance and Targeting (C5ISRT) framework acknowledges its strategic relevance in shaping military strategies and responses in an increasingly complex and interconnected world. This paradigm shift highlights that the modern landscape extends beyond physical and virtual theatres to encompass the cognitive and social dimensions that define human interactions and conflicts.

Alphacoronaviruses from bats captured in European Russia in 2015 and 2021 are closely related to those of Northern Europe

IntroductionBats are considered the natural reservoirs of several viruses including coronaviruses (CoVs), two genera of which, alpha- and betacoronaviruses, infect humans. Despite widespread screening of bat samples for novel viruses, information on the diversity of coronaviruses in bats inhabiting Russian territory remains scarce. Here, we analyzed the presence and diversity of alphacoronaviruses (Alpha-CoVs) in bats from European Russia.MethodsFourty three fecal samples from bats of 8 species: P. nathusii, P. kuhlii, M. brandtii, M. daubentonii, N. noctula, V. murinus, M. dasycneme, and P. auritus were taken to study.Results and discussionWe detected Alpha-CoV RdRp gene fragments in 30% (13/43) of samples examined in 75% (6/8) of species sampled. Phylogenetic analysis of RdRp showed that most of the identified Alpha-CoV sequences fall into clades within the Pedacovirus subgenus, with minor clusters of nyctacoviruses or myotacoviruses. We assert that closely related pedacoviruses have been circulating for a long time (from 2015 to 2021) in a large region from European Russia to Northern Europe. We propose that closely related pedacoviruses collected from common areas represent a separate species, which we name NE-Alpha coronavirus, with its host being bats of the genus Pipistrellus that inhabit a region from the European part of Russia to Northern Europe. Among the animals sampled, 4.6% (2/43) carried two Alpha-CoVs related to different subgenera (pedacovirus/myotacovirus or pedacovirus/nyctacovirus) simultaneously. We confirmed the presence of two different Alpha-CoV subgenera related to pedacovirus and nyctacovirus in P. kuhlii captured in 2021 and kept in captivity using whole genome sequencing of these viruses. The presence of two or more coronaviruses in one individual animal host is an essential prerequisite for recombination to occur. We also obtained two Alpha-CoV whole genomes from two specimens of P. nathusii captured in 2015. The genomic organization of BatCoV/MOW15-21 and BatCoV/MOW15-23 was similar to other Alpha-CoVs, but the assembled genomes contained a long insertion in the ORF1ab gene which has not been described in other Alpha-CoVs, except for a single sequence from P. nathusii captured in the Netherlands. We propose that the insertion encodes a previously undescribed domain of unknown function, probably related to the SEA domain superfamily.

Structural Basis for Multivalent MUC16 Recognition and Robust Anti-Pancreatic Cancer Activity of Humanized Antibody AR9.6.

Mucin-16 (MUC16) is a target for antibody-mediated immunotherapy in pancreatic ductal adenocarcinoma (PDAC) among other malignancies. The MUC16-specific monoclonal antibody AR9.6 has shown promise for PDAC immunotherapy and imaging. Here, we report the structural and biological characterization of the humanized AR9.6 antibody (huAR9.6). The structure of huAR9.6 was determined in complex with a MUC16 SEA (Sea urchin sperm, Enterokinase, Agrin) domain. Binding of huAR9.6 to recombinant, shed, and cell-surface MUC16 was characterized, and anti-PDAC activity was evaluated in vitro and in vivo. HuAR9.6 bound a discontinuous, SEA domain epitope with an overall affinity of 88 nmol/L. Binding affinity depended on the specific SEA domain(s) present, and glycosylation modestly enhanced affinity driven by favorable entropy and enthalpy and via distinct transition state thermodynamic pathways. Treatment with huAR9.6 reduced the in vitro growth, migration, invasion, and clonogenicity of MUC16-positive PDAC cells and patient-derived organoids (PDO). HuAR9.6 blocked MUC16-mediated ErbB and AKT activation in PDAC cells, PDOs, and patient-derived xenografts and induced antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity. More importantly, huAR9.6 treatment caused substantial PDAC regression in subcutaneous and orthotopic tumor models. The mechanism of action of huAR9.6 may depend on dense avid binding to homologous SEA domains on MUC16. The results of this study validate the translational therapeutic potential of huAR9.6 against MUC16-positive PDACs.

A Revised Molecular Model of Ovarian Cancer Biomarker CA125 (MUC16) Enabled by Long-read Sequencing.

Despite its crucial role in clinical management of ovarian cancer, the exact molecular sequence and structure of the biomarker, CA125, are not defined. Here, we combine long-read sequencing, mass spectrometry, and in silico modeling to provide the foundational dataset for a more complete characterization of the CA125 epitope.

Development of a Numerical Prediction Model for Marine Lower Atmospheric Ducts and Its Evaluation across the South China Sea

The Coupled Ocean–Atmosphere–Wave–Sediment Transport (COAWST) model serves as the foundation for creating a forecast model to detect lower atmospheric ducts in this study. A set of prediction tests with different forecasting times focusing on the South China Sea domain was conducted to evaluate the short-term forecasting effectiveness of lower atmospheric ducts. The assessment of sounding observation data revealed that the prediction model performed well in predicting the characteristics of all types of ducts. The mean values of the forecasting errors were slightly lower than the reanalysis data but had lower levels of correlation coefficients. At an altitude of about 2000 m, the forecasted error of modified atmospheric refractivity reached peak values and then decreased gradually with increasing altitude. The accuracy of forecasted surface ducts was higher than that of elevated ducts. Noticeable land–sea differences were identified for the spatial distributions of duct characteristics, and the occurrence rates of both the surface and elevated ducts were high at sea. As for the differences among the forecasts of 24, 48, and 72 h ahead, the differences primarily occurred at altitude levels below 20 m and 500 m~1500 m, which are consistent with the differences in the duct height.

Development of New Models of Oral Mucosa to Investigate the Impact of the Structure of Transmembrane Mucin-1 on the Mucosal Pellicle Formation and Its Physicochemical Properties.

The mucosal pellicle (MP) is a biological film protecting the oral mucosa. It is composed of bounded salivary proteins and transmembrane mucin MUC1 expressed by oral epithelial cells. Previous research indicates that MUC1 expression enhances the binding of the main salivary protein forming the MP, MUC5B. This study investigated the influence of MUC1 structure on MP formation. A TR146 cell line, which does not express MUC1 natively, was stably transfected with genes coding for three MUC1 isoforms differing in the structure of the two main extracellular domains: the VNTR domain, exhibiting a variable number of tandem repeats, and the SEA domain, maintaining the two bound subunits of MUC1. Semi-quantification of MUC1 using dot blot chemiluminescence showed comparable expression levels in all transfected cell lines. Semi-quantification of MUC5B by immunostaining after incubation with saliva revealed that MUC1 expression significantly increased MUC5B adsorption. Neither the VNTR domain nor the SEA domain was influenced MUC5B anchoring, suggesting the key role of the MUC1 N-terminal domain. AFM-IR nanospectroscopy revealed discernible shifts indicative of changes in the chemical properties at the cell surface due to the expression of the MUC1 isoform. Furthermore, the observed chemical shifts suggest the involvement of hydrophobic effects in the interaction between MUC1 and salivary proteins.

Conceptual Approach to Multi-Domain Operations

This article delves into multi-domain operations in the context of future warfighting. A multi-domain approach to operations is being progressively implemented in allied nations and within NATO, as well. It should also become a core interest for the Czech Republic, even if the sea and space domains do not represent the crucial area of potential operational engagement of the Army of the Czech Republic. The complexity of operations in a complex operating environment requires mutual interaction across all operational domains. The integration of the physical and virtual spheres of operations suggests that operations planning cannot be strictly oriented to individual operational domains. The interaction between operational domains must be perceived as a sine qua non for effective planning and conducting military operations. This is also a fundamental prerequisite for the successful activity of forces in a complex operating environment.

Challenges and Opportunities in Meeting the Needs of IKN Weapon Systems in the Era of Modern Weaponry Technologies

The relocation of Indonesia's capital city to East Kalimantan has implications for defense and security. This research analyzes the challenges and opportunities of fulfilling the main equipment of the National Capital Integrated Coastal Development (IKN) defense system in the era of modern weaponry technology. Through the Systematic Literature Review (SLR) method, the evolution of warfare from the first generation to the information and cyber era is analyzed in relation to IKN. The research also highlights the development of weaponry technology and its impact on the IKN transition, as well as the urgency of fulfilling modern defense equipment and integrating advanced technology. The results indicate that the relocation of IKN requires strategic adaptation to the evolution of warfare and the use of new technologies in defense. It is crucial to comprehensively fulfill the needs of defense equipment in the air, land, sea, and cyber domains to maintain the sovereignty and security of IKN. With the integration of advanced technology and wise planning, the relocation of IKN can be approached with thorough preparation and an effective defense system. In facing these complex dynamics, international collaboration efforts and adaptation of defense policies become important to respond to global challenges and generate appropriate solutions.

Developing a Comparative Effective Methodology forTechnology Usability During a Simulated Casualty Event.

Future combat environments will be complex, making effective care for multi-domain battlefield injuries more challenging. Technology and resources are essential to reduce provider burden enabling more accurate assessments, decision-making support, expanded treatment, and outcome improvements. Experimentation exercises to evaluate concepts and technologies to incorporate into the Army's future force ensure rapid and continuous integration across air, land, sea, space, and cyberspace domains to overmatch adversaries. A medical lane was first integrated on the communications networks for experimentation in 2022. We describe a project to develop a method for empirically comparing devices intended to support combat casualty care through high-fidelity simulation in preparation for an Army experimentation exercise. Six medics participated in a series of high-fidelity simulation medical casualty injury scenarios with and without technology devices. The participants provided usability information about their care delivery experiences using the System Usability Scale and Adapted Telehealth Usability Questionnaire-Telemedicine and Advanced Technology Research Command and qualitative feedback. A comparative effectiveness design compared the devices regarding their usability, size, weight, and power with the addition of cost, connectivity, and cyber security, and the qualitative feedback this methodology holistically assessed the technologies as they were applied in the combat casualty care scenario. Results were used by decision makers to determine technology inclusion in experimentation exercise, develop proof of concept methodology to scale for the exercise, and provide technology developers feedback for iterative updates of their devices before participation in experimentation exercise. This project supports the body of simulation studies conducted to understand combat casualty care. It is one of few empirical medical technology assessments with medical personnel end user input that has been reported. The methodology incorporates a user-centered design for rapid technology improvements before fielding.

Bacterial SEAL domains undergo autoproteolysis and function in regulated intramembrane proteolysis

Gram-positive bacteria use SigI/RsgI-family sigma factor/anti-sigma factor pairs to sense and respond to cell wall defects and plant polysaccharides. In Bacillus subtilis, this signal transduction pathway involves regulated intramembrane proteolysis (RIP) of the membrane-anchored anti-sigma factor RsgI. However, unlike most RIP signaling pathways, site-1 cleavage of RsgI on the extracytoplasmic side of the membrane is constitutive and the cleavage products remain stably associated, preventing intramembrane proteolysis. The regulated step in this pathway is their dissociation, which is hypothesized to involve mechanical force. Release of the ectodomain enables intramembrane cleavage by the RasP site-2 protease and activation of SigI. The constitutive site-1 protease has not been identified for any RsgI homolog. Here, we report that RsgI's extracytoplasmic domain has structural and functional similarities to eukaryotic SEA domains that undergo autoproteolysis and have been implicated in mechanotransduction. We show that site-1 proteolysis in B. subtilis and Clostridial RsgI family members is mediated by enzyme-independent autoproteolysis of these SEA-like domains. Importantly, the site of proteolysis enables retention of the ectodomain through an undisrupted β-sheet that spans the two cleavage products. Autoproteolysis can be abrogated by relief of conformational strain in the scissile loop, in a mechanism analogous to eukaryotic SEA domains. Collectively, our data support the model that RsgI-SigI signaling is mediated by mechanotransduction in a manner that has striking parallels with eukaryotic mechanotransducive signaling pathways.

Glycosylated extracellular mucin domains protect against SARS-CoV-2 infection at the respiratory surface.

Mucins play an essential role in protecting the respiratory tract against microbial infections while also acting as binding sites for bacterial and viral adhesins. The heavily O-glycosylated gel-forming mucins MUC5AC and MUC5B eliminate pathogens by mucociliary clearance. Transmembrane mucins MUC1, MUC4, and MUC16 can restrict microbial invasion at the apical surface of the epithelium. In this study, we determined the impact of host mucins and mucin glycans on epithelial entry of SARS-CoV-2. Human lung epithelial Calu-3 cells express the SARS-CoV-2 entry receptor ACE2 and high levels of glycosylated MUC1, but not MUC4 and MUC16, on their cell surface. The O-glycan-specific mucinase StcE specifically removed the glycosylated part of the MUC1 extracellular domain while leaving the underlying SEA domain and cytoplasmic tail intact. StcE treatment of Calu-3 cells significantly enhanced infection with SARS-CoV-2 pseudovirus and authentic virus, while removal of sialic acid and fucose from the epithelial surface did not impact viral entry. In Calu-3 cells, the transmembrane mucin MUC1 and ACE2 are located to the apical surface in close proximity and StcE treatment results in enhanced binding of purified spike protein. Both MUC1 and MUC16 are expressed on the surface of human organoid-derived air-liquid interface (ALI) differentiated airway cultures and StcE treatment led to mucin removal and increased levels of SARS-CoV-2 replication. In these cultures, MUC1 was highly expressed in non-ciliated cells while MUC16 was enriched in goblet cells. In conclusion, the glycosylated extracellular domains of different transmembrane mucins might have similar protective functions in different respiratory cell types by restricting SARS-CoV-2 binding and entry.

High-resolution GEOS-Chem model for Indian monsoon region: Seasonal cycle and budget of tropospheric CO2

A nested GEOS-Chem atmosphere transport model has been set up for the Indian monsoon region (40° E-110° E & 15° S–45° N at 0.250X0.3125°) spatial resolution to study the variability of the tropospheric CO2 over India and surrounding oceans during 2012–2020. The model has been constrained by the influxes of CO2 at its four lateral boundaries derived from the GEOS-Chem global simulations and surface fluxes of CO2 over the land and oceans based on historical databases. It is initialized from a known state and driven by the GEOS reanalysis's meteorological forcing. Model simulations were evaluated at three in situ measurement locations and with respect to the satellite retrievals from OCO-2 and GOSAT. Model parameters were tuned to have reasonable solutions to resolve observed latitudinal gradient and seasonal and inter-annual variability. Three additional model runs were made to evaluate the role of long-range transport, net terrestrial ecosystem exchange, air-sea fluxes, and regional emissions in controlling seasonal oscillations of tropospheric CO2 over the study region. It revealed that the net terrestrial ecosystem exchanges are responsible for 35% control in the surface layer CO2 seasonal tendency for India and the Bay of Bengal domains, and 10% for the Arabian Sea. The air-sea fluxes have 8% control on the CO2 seasonal tendency for the Arabian Sea and Bay of Bengal and 5% for India. The anthropogenic emissions have 4% control for India and <1% for the Arabian Sea, and 9% for the Bay of Bengal. The long-range transport, as the residual, is responsible for 60% variability in the seasonal cycle of CO2 over India and the Bay of Bengal and 75% for the Arabian Sea domain.

Detect, Deny, Degrade, Disrupt, Destroy, Deceive: which is the greatest in OCO?

In the cyber kill chain literature, possible courses of action are listed as detect, deny, degrade, disrupt, destroy, and deceive (a.k.a. “the 6Ds”). These verbs denote defensive action to be taken against an intruder. By comparison, military doctrine for cyberspace operations encompasses cyberspace exploitation and attack, as well as defence. The question arises whether the 6Ds are also applicable to offensive action, i.e. exploitation and attack, or whether additional action verbs are needed. Military doctrine is evolving towards all-domain operations, in which action in cyberspace is integrated with action in the physical domains of land, sea, air, and space. This prompts the question as to whether the 6Ds are also suited to action in a physical domain. A pilot study of actual military operations that integrated cyber and physical action suggests that deception, delay, and denial of organisational and cyber entities is suited to cyber action, while seizure, capture, and destruction of physical entities is suited to physical action. Preference among action verbs may indicate when it is best to engage targets using cyber or physical resources and which action is preferred. This paper identifies which action verbs are best suited to offensive cyber operations in the context of all-domain operations. The paper reviews related theory on cyberspace and the cyber kill chain. It identifies action verbs in US Department of Defense (DoD) doctrine on information and cyberspace operations, comparing them to those in the US DoD Dictionary of Military and Associated Terms. After discussing the findings, the paper draws conclusions and recommends further work.

Screening, classification, capacity estimation and reservoir modelling of potential CO2 geological storage sites in the NW Adriatic Sea, Italy

In Italy, the central-northern Apennines and Adriatic Sea domains have been identified as potential CO2 storage areas. Although capacity estimates have been undertaken at a regional scale, more detailed research is required to reduce uncertainty. The present study helps address this gap by conducting a detailed screening of a representative area in the north-western Adriatic Sea for suitable CCS sites, using an extensive dataset composed of both public and private seismic and borehole data. Work included selecting suitable sites based on proposed selection criteria, calculating their theoretical and effective storage capacities applying a probabilistic approach, and then performing dynamic flow modelling on one of the selected sites that is representative of fractured carbonate reservoirs. Based on the screening results, a total of 21 out of 38 structures were selected as having the required characteristics suitable for CO2 storage. As expected, the clastic rock reservoirs have the greatest potential capacity, although some fractured carbonate reservoirs did yield important volumes due to their dual porosity / permeability characteristics. Modelling of one such site highlighted the complex interplay between primary and secondary porosity / permeability on plume migration and capacity estimation. Our results highlight the need to integrate analytical calculations with dynamic simulations for more detailed, site-specific studies to improve the estimates of CO2 storage capacity.

Crystal Structure of the Extracellular Domains of GPR110

Adhesion G protein-coupled receptors (aGPCRs) play a pivotal role in human immune responses, cellular communication, organ development, and other processes. GPR110 belongs to the aGPCR subfamily VI and was initially identified as an oncogene involved in lung and prostate cancers. GPR110 contains tandem adhesion domains at the extracellular region that mediate inter-cellular signaling. However, the structural organization and signaling mechanism for these tandem domains remain unclear. Here, we report the crystal structure of a GPR110 fragment composing the SEA, HormR, and GAIN domains at 2.9 Å resolution. The structure together with MD simulations reveal rigid connections between these domains that are stabilized by complementary interfaces. Strikingly, we found N-linked carbohydrates attached to N389 of the GAIN domain form extensive contacts with the preceding HormR domain. These interactions appear to be critical for folding, as removal of the glycosylation site greatly decreases expression of the GPR110 extracellular fragment. We further demonstrate that the ligand synaptamide fits well within the hydrophobic pocket occupied by the Stachel peptide in the rest state. This suggests that the agonist may function by removing the Stachel peptide which in turn redocks to the orthosteric pocket for receptor activation. Taken together, our structural findings and analyses provide novel insights into the activation mechanism for aGPCRs.

Tsunami free oscillations excited by potential local earthquake sources in Bohai Sea, China

The Bohai Sea is the most seismic active region offshore mainland China. Its coastal region is highly populated. The present work proposed a comprehensive scheme for identifying and examining free oscillations with limited number of tide gauges in an irregular semi-enclosed basin. We combined modal analysis, spectral analysis, and multiple-scenario simulation to investigate tsunami free oscillations in the Bohai Sea Basin and three local bays including Liaodong Bay, Bohai Bay, and Laizhou Bay. The Bohai Sea had broadband periods of free oscillations ranging from 228 to 30 min. There existed both along-shore and cross-shore oscillations in most eigenmodes. The ultra-long dominant periods above 50 min were attributed to the shallow water depth (<20 m). Some free oscillation modes appeared in both the Bohai Sea and local bay domains. In addition, the spatial patterns of dominant eigenmodes were characterized and validated by performing spectral analysis for an Mw 7.8 tsunami scenario. The energy was mainly concentrated in the first three modes (171, 146, and 93 min). This is the first study to characterize the areas prone to tsunami hazards in the Bohai Sea, and the methodology can be further extended to other coastal regions.

Emergence of MUC1 in Mammals for Adaptation of Barrier Epithelia.

Simple SummaryThe evidence reviewed here indicates that MUC1-C evolved in mammals to promote inflammatory adaptation in barrier tissues that extends to resident stem cells and immune cells. Inflammatory memory is an essential process that protects barrier niches against future insults. Evolutionary adaptations arising from natural selection, as for example the MUC1 gene, can be beneficial for survival. However, prolonged activation of MUC1-C in settings of chronic inflammation represents an adverse adaptation that promotes cancer.The mucin 1 (MUC1) gene was discovered based on its overexpression in human breast cancers. Subsequent work demonstrated that MUC1 is aberrantly expressed in cancers originating from other diverse organs, including skin and immune cells. These findings supported a role for MUC1 in the adaptation of barrier tissues to infection and environmental stress. Of fundamental importance for this evolutionary adaptation was inclusion of a SEA domain, which catalyzes autoproteolysis of the MUC1 protein and formation of a non-covalent heterodimeric complex. The resulting MUC1 heterodimer is poised at the apical cell membrane to respond to loss of homeostasis. Disruption of the complex releases the MUC1 N-terminal (MUC1-N) subunit into a protective mucous gel. Conversely, the transmembrane C-terminal (MUC1-C) subunit activates a program of lineage plasticity, epigenetic reprogramming and repair. This MUC1-C-activated program apparently evolved for barrier tissues to mount self-regulating proliferative, inflammatory and remodeling responses associated with wound healing. Emerging evidence indicates that MUC1-C underpins inflammatory adaptation of tissue stem cells and immune cells in the barrier niche. This review focuses on how prolonged activation of MUC1-C by chronic inflammation in these niches promotes the cancer stem cell (CSC) state by establishing auto-inductive nodes that drive self-renewal and tumorigenicity.