Sea Buckthorn Oil Research Articles

Sea Buckthorn Oil Promotes the PI3K-Akt-ERK Signaling Pathway and Macrophage M2 Polarization to Reduce Radiation-induced Skin Injury.

In this work, we explored the role and mechanism of sea buckthorn oil in reducing radiation-induced skin damage. The radiation-induced rat skin injury model was established using strontium-90. Rats were treated with sea buckthorn oil twice a day postirradiation, and skin damage was observed at different times and evaluated using an injury score. Skin pathological changes were observed using hematoxylin and eosin (H&E) staining. Western blotting and immunohistochemistry were used to detect the expression of vascular growth and pathway proteins. ELISA was used to detect the secretion level of inflammatory factors. Immunohistochemistry was used to detect macrophage polarization marker proteins. We found that sea buckthorn oil can alleviate radiation-induced skin damage, accelerate skin vascular regeneration, and promote the up-regulation of vascular endothelial growth factor (VEGF) and its receptor (VEGFR). These results demonstrate the beneficial effects of sea buckthorn oil on radiation-induced skin damage. Furthermore, the levels of IL-1β and TNF-α in the sea buckthorn oil treatment group were significantly lower than those in the control group, while the levels of IL-4 and IL10 were significantly higher (P < 0.05). CD206 expression also increased in the sea buckthorn oil treatment group, while CD16 expression decreased compared to the control group (P < 0.05). Western blotting showed that PI3K, Akt and ERK expression increased in the sea buckthorn oil treatment group (P < 0.05). The beneficial effect of sea buckthorn oil in reducing the inflammatory response in irradiated rats was diminished when they were treated with PI3K inhibitor. We conclude that sea buckthorn oil may regulate macrophage M2 polarization by increasing the PI3K-Akt-ERK signaling pathway, thereby inhibiting the inflammatory response and promoting skin vascular regeneration to prevent and treat radiation-induced skin damage.

Revealing the effect of sea buckthorn oil, fish oil and structured lipid on intestinal microbiota, colonic short chain fatty acid composition and serum lipid profiles in vivo

In this study, the effects of sea buckthorn oil (SBO), fish oil (FO) and an enzymatically synthesized structured lipid (SL) on serum, short-chain fatty acids (SCFAs) and intestinal microbiota in Sprague–Dawley (SD) rats were investigated. The results demonstrated that FO, SBO, and SL effectively reduced the levels of high-density lipoprotein cholesterol and low-density lipoprotein cholesterol in the serum of SD rats. SBO increased serum triglyceride levels, while FO elevated total cholesterol levels. Furthermore, all three dietary lipids decreased short-chain fatty acid production and enhanced intestinal microbiota diversity. FO increased the abundance of intestinal microbiota including Romboutsia, Lactobacillus, Escherichia-Shigella, and Lachnospiraceae_NK4A136_group. Conversely, all three dietary lipids reduced the abundance of Klebsiella and Blautia. These findings provide a foundation for understanding the functionality of SBO and FO as well as their potential application in synthesizing novel SLs to regulate intestinal microbiota.Graphical

Study of the epithelial state of the oral mucosa in patients with recurrent aphthous stomatitis

Relevance. Recurrent aphthous stomatitis (RAS) is one of the most common diseases affecting the oral mucosa. It is characterized by the recurrent appearance of ulcers (aphthae) and a prolonged course with periodic exacerbations.Purpose. To study the condition of the oral mucosa epithelium in patients with recurrent aphthous stomatitis undergoing treatment with antiseptics and reparative agents.This study examined samples of buccal epithelium from 64 patients, who were randomly divided into two equal groups: the first group used chlorhexidine as a mouthwash (control group); the second group used octenidine + phenoxyethanol for 7 days. For pain relief, patients in both groups applied a gel containing lidocaine + chamomile extract. After 7 days, the therapy continued with reparative agents (a hydrogel-based material with sodium alginate, Derinat, and lidocaine, deproteinized calf blood extract, and sea buckthorn oil). Each group was further divided into three subgroups for this part of the therapy. The condition of the buccal epithelium was assessed three times: before treatment, on the 7th day, and on the 21st day of therapy. Buccal epithelium preparations were prepared and analyzed using standard methods.Results. The analysis of cytogenetic and karyological indicators of buccal epithelial cells in patients with CRAS revealed that, at the peak of the inflammatory process, cells with micronuclei and various forms of nuclear protrusions were more frequently observed. During therapy, there was a notable reduction in buccal epithelial cells exhibiting these pathological features.Conclusion. Before therapy, patients with RAS exhibited cytogenetic instability and various karyological anomalies in the buccal epithelial cells of the oral mucosa. These anomalies included significantly higher occurrences of micronuclei, karyopyknosis, karyolysis, nuclear vacuolization, intercellular bridges, and chromatin condensation. During the different therapy regimens for RAS, normalization of the epithelial state of the oral mucosa was observed, with the most pronounced improvements seen with the use of octenidine + phenoxyethanol in combination with a hydrogel-based material containing sodium alginate, Derinat, and lidocaine.

Compositional Characterization and Bioactive Potential of Two Bee Formulas with the Addition of Natural Oils

Bee products and natural oils are appreciated due to their beneficial effects on health, which are strongly related to several pharmacological and biological activities. The present study aimed to assess the bioactive potential of two bee products with the addition of natural oils, the first product consisting of honeydew honey, soft propolis extract and Thymus sp. (thyme) essential oil, whereas the second product comprises honeydew honey and Hippophae rhamnoides L. (sea buckthorn) oil. In order to reach the proposed goal, the following analyses were carried out: determination of the physico-chemical parameters of the honeydew honey sample, used as a common basis for the two products; compositional analysis of sea buckthorn oil (total carotenoids, fatty acids, tocopherols, sterols) and thyme essential oil (organic compounds); evaluation of the total polyphenols and flavones/flavonols content; evaluation of the antioxidant capacity (DPPH and FRAP methods). The results highlighted the superior quality and compliance with the legal framework of the honeydew honey sample and natural oils. Moreover, the two investigated products exhibited a rich content of bioactive compounds as well as good antioxidant activity.

Morphological changes in gum tissue during thiram intoxication followed by correction with resveratrol and sea buckthorn oil

Background: The widespread use of pesticides can lead to a violation of the ecological balance between the environment and the human body, provoking the development of oxidative stress, which leads to an increase in toxic forms of damage to organs and systems of the macroorganism. In order to suppress oxidative stress that develops in many pathological processes, it is advisable to use antioxidants that inhibit the formation of free radicals. The aim of the study: To study the morphological changes in gum tissues in case of thiram intoxication followed by correction with resveratrol and sea buckthorn oil. Materials and methods: The experiment was performed on 50 rats that received the fungicide thiram for 28 days, followed by correction with resveratrol or sea buckthorn oil. To assess the severity of inflammatory changes in histological preparations, morphofunctional changes in the mucosal epithelium and the cellular composition of the subepithelial layer were analysed. Fibroblastic differon cells, macrophages and inflammatory cells of granulocytes and lymphocytes were differentiated by karyological signs, and the thickness of the epithelial layer, the area occupied by the fibrous, vascular and cellular components were measured. Results: In the experimental groups “Thiram 28 days” and “Thiram+standard diet” in all rats, the development of an inflammatory reaction of the gum tissue was observed, manifested in hyperkeratosis and an increase in the thickness of the epithelium up to 173.438 microns and 230.568 microns, diffuse polymorphocellular infiltration of the subepithelial layer mainly due to granulocytes to 21.40 and 21.92, respectively, expansion of the lumen and blood filling of the vessels of the microvasculature. After the use of resveratrol and sea buckthorn oil, the thickness of the epithelial layer was restored to 114,07 and 190,03 microns, respectively, interstitial edema and cell infiltration of the subepithelial layer decreased. At the same time, due to migration and increase of fibroblastic differon cells (28.96 and 29.44), neocollagenogenesis processes and an increase in the fibrous component were observed up to 65.62 and 33.69 after application of resveratrol and sea buckthorn oil, respectively. Conclusion: The effectiveness of monotherapy with resveratrol and sea buckthorn oil in normalizing the morphology of gum tissue has been proven. At the same time, resveratrol has both an anti-inflammatory effect and a high regenerative effect.

Development of Novel Cost Efficient Process for Extraction of Sterols and Tocopherols from Wild Plant Seed

The present work focuses on the compositional characterization of tamarind and sea buckthorn seed oils, which have a wide range of uses in the cosmetic, pharmaceutical and food sectors. The characterization includes the analysis of tocopherols, sterols and fatty acid profile in both oils. The tamarind and sea buckthorn oils were converted to fatty acid methyl ester and the fatty acid composition was analyzed by using gas chromatography technique. The quantitative and qualitative analysis of tocopherols performed using high performance liquid chromatography. This study reveals that the oil samples consist of a variety of saturated and unsaturated fatty acids, such as myristic acid (0 and 0.4%), palmitic acid (11 and 30.5%), palmitoleic (0 and 39.6%) stearic acid (0 and 0.5%), oleic acid (18.3 and 26.1%), linoleic acid (45.8 and 2.1%), linolenic acid (4.1% and 0.5%), arachidic acid (1.8% and 0%) behenic acid (9.6 and 0%) and lignoceric acid (9.4 and 0%). Additionally, the analysis detects various tocopherol components, including α-tocopherol (21.4 and 32.6%), β-tocopherol (14.49 and 7.24%), γ-tocopherol (53.72 and 45.66%), δ-tocopherol (9.64 and 9.11%) and α-tocotrienol (0 and 5.13%) in tamarind and sea buckthorn oil, respectively.

Comfort Properties and Antimicrobial Activity of Cotton and Nylon/PU Knits Treated with Microcapsules Containing Sea Buckthorn Oil

This study was performed to investigate comfort properties and antimicrobial activity of cotton and nylon knits treated with microcapsules containing sea buckthorn fruit oil reported as having antimicrobial efficacy depending on concentration of microcapsules and treatment types. Chitosan-arabic gum microcapsules containing sea buckthorn fruit oil were prepared and they were treated on cotton and nylon/PU knit respectively by an experimental design of 2 different treatment types (bath immersion method and screen printing method) Χ 5 different concentration levels of microcapsules (20, 30, 40, 50, and 60% on the weight of fabric). As comfort properties, stiffness, water-vapor permeability, and air permeability were measured objectively. Antimicrobial activity was evaluated by calculating the percentage reduction of the bacterium. As results, stiffness values of the treated knits were slightly higher than those of untreated ones, which was thought not to affect touch in wear. The values of water-vapor permeability and air permeability of both cotton and nylon after the treatment were not significantly decreased. These results means that microcapsules loaded on the knits might not worsen their wear comfort. As for antimicrobial activity, more than 90% of bacterial reduction rates against S. Aureus were shown in cotton jersey by screen printing even after repeated laundry while those was found in nylon/PU tricot by bath immersion. From these results, it was concluded that chitosan-arabic gum microcapsule containing sea buckthorn fruit oil could be utilized for textiles to provide antimicrobial activity.

Assessment of the Nutritional Potential and Resistance to Oxidation of Sea Buckthorn and Rosehip Oils

Cold-pressed oils from non-typical materials, such as wild rose and sea buckthorn, should meet certain requirements to be approved for consumption. The aim of this study was to evaluate the nutritional potential and selected quality parameters with particular emphasis on oxidative stability of two commercially available cold-pressed oils: rose hip oil and sea buckthorn oil. The fatty acid composition, including nutritional indexes (polyunsaturated fatty acid/saturated fatty acid ratio PUFA/SFA; atherogenicity index IA is as follows: hypocholesterolemic/hypercholesterolemic index HH; health-promoting index HPI), positional distribution of fatty acids, melting profile and oxidative stability were analyzed. The tested oils were characterized by a high content of unsaturated fatty acids, which were mainly represented by oleic and linoleic acids. In the case of one of the analyzed rosehip oil oils (R1), α-linolenic acid was also found at the level of 26%, which contributed to obtaining the recommended ratio of omega-6 to omega-3 fatty acids. The lowest value of IA and the highest values of HH and HPI were also recorded for R1 rosehip oil. This oil was also characterized by the highest resistance to oxidation, as indicated by induction times measured at various temperatures. The distribution of fatty acids in triacylglycerols molecules in the analyzed oils was typical for vegetable oils. In the case of tested oils, similar melting profiles with endothermic peaks recorded at negative temperatures, which resulted from the presence of triacylglycerol fractions with a high proportion of mono- and polyunsaturated fatty acids, were observed.

Optimization of ultrasonic-assisted solvent extraction process for sea buckthorn (Hippophae rhamnoids L.) oil using response surface methodology

Optimization of ultrasonic-assisted solvent extraction process for sea buckthorn (Hippophae rhamnoids L.) oil using response surface methodology

The impact of selected xanthophylls on oil hydrolysis by pancreatic lipase: in silico and in vitro studies

Lipase inhibition is one of the directions to control obesity. In vitro assays have confirmed the inhibitory effect of selected xanthophylls, including astaxanthin, fucoxanthinol, fucoxanthin, and neoxanthin. Similarly, an in-silico study also demonstrated the successful inhibition of pancreatic lipase by astaxanthin. Unfortunately, the efficacy of these protocols in the emulsion state typical of lipid digestion remains untested. To address this issue, the current study employed the pH–stat test, which mimics lipid digestion in the gastrointestinal tract, to evaluate native and prepared sea buckthorn and rapeseed oils with varying xanthophyll contents from 0 to 1400 mg/kg oil. Furthermore, a molecular docking of zeaxanthin and violaxanthin (commonly found in plant-based foods), astaxanthin (widely distributed in foods of marine origin) and orlistat (approved as a drug) was performed. The in-silico studies revealed comparable inhibitory potential of all tested xanthophylls (variation from − 8.0 to − 9.3 kcal/mol), surpassing that of orlistat (− 6.5 kcal/mol). Nonetheless, when tested in an emulsified state, the results of pH–stat digestion failed to establish the inhibitory effect of xanthophylls in the digested oils. In fact, lipolysis of native xanthophyll-rich sea buckthorn oil was approximately 22% higher than that of the xanthophyll-low preparation. The key insight derived from this study is that the amphiphilic properties of xanthophylls during the digestion of xanthophyll-rich lipids/meals facilitate emulsion formation, which leads to enhanced fat lipolysis.

Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro.

Skeletal muscle is the main edible part of meat products, and its development directly affects the yield and palatability of meat. Sea buckthorn oil (SBO) contains plenty of bioactive substances and has been recognized as a potential functional food product. The study aimed to explore the effects and possible mechanisms of SBO on sheep primary myoblast proliferation and myogenic differentiation. The results implied that SBO exhibited a pro-proliferative effect on primary myoblasts, along with up-regulated proliferating cell nuclear antigen (PCNA) and Cyclin D1/cyclin-dependent kinase 4 (CDK4) abundances. And, SBO promoted myotube formation by increasing the expression of myogenin. Meanwhile, we found that SBO inhibited the expression of miRNA-292a. Moreover, the regulatory effect of SBO on myogenic differentiation of myoblasts was attenuated by miRNA-292a mimics. Of note, SBO activated protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway and augmented glucose uptake and glucose transporter 4 (GLUT4) content, which might be attributed to AMP-activated protein kinase (AMPK) activation. Additionally, the results were shown that SBO increased the abundance of antioxidative enzymes, including glutathione peroxidase 4 (Gpx4) and catalase. In summary, these data suggested that SBO regulated the proliferation and myogenic differentiation of sheep primary myoblasts in vitro, which might potentiate the application of SBO in muscle growth.

The impact of oral sea-buckthorn oil on skin, blood markers, ocular, and vaginal health: A randomized control trial

Sea buckthorn have been used for centuries as food and traditional medicine in Asia and Europe. This study evaluated the effects of a nutraceutical consisting of sea buckthorn oil as a functional supplement (Omegia™ sea buckthorn oil). Subjects were randomly divided into two groups and were given capsules containing or without sea buckthorn oil. Skin elasticity, color, collagen, pore, and redness were measured using skin assessment instruments. Blood samples were taken to assess serum antioxidant levels by using ELISA (enzyme-linked immunosorbent assay) kit. Additionally, a questionnaire survey was conducted to gather information on subjects' experiences in eye discomfort and vaginal dryness issues. After 12 weeks, skin parameters showed improvement, and participants reported relief from dry eyes and vulvar issues. This study highlights the potential of Omegia™ supplements demonstrating the ability to delay skin aging, increase high-density lipoprotein cholesterol, and antioxidant enzyme activity when used continuously for a period of 12 weeks.

Fabrication and characterization of shikonin and sea buckthorn endowed nanoemulsion reinforcing elecrospun scaffolds for treatment of diabetic wounds

Electrospun polymeric nanofibers represent a novel class of wound dressing owing to their structural resemblance with the natural extracellular matrix within which pro and anti-apoptotic functions can be orchestrated comprehensively resulting in effective wound management especially in case of diabetic wounds. Recent epidemiological reports demonstrate the role of matrix metalloproteinases (MMPs) as a clear indicator of recalcitrant healing in diabetic wounds that can specifically ablate the growth factors and other pro-healing molecules hindering the healing process. Beguiling the anti-inflammatory, antimicrobial, anti-diabetic and MMP-9 modulatory properties of naphthoquinone pigments, shikonin has enticed interest of researchers in diabetic wound management. Poor solubility and photosensitivity demands novel delivery of this compound for better therapeutic efficacy. The study, thus, aims to develop reinforced shikonin and sea buckthorn oil loaded nanoemulsion (SS-NE) based chitosan scaffolds by incorporation of SS-NE as nanofiller in fibers. Subsequent characterization confirmed the stability and encapsulation of shikonin in prepared scaffold system. The results of burn wound model in diabetic Wistar rats (Type 2 diabetes) indicated that the micro-nanostructure of the fiber mats was able to promote wound healing by substantially downregulating the level of MMP-9 and stimulating collagen deposition and re-epithelization. The salient findings of the present study proposed that the bioenthused asymmetric wettable scaffolds can be employed as a propitious delivery system for treatment of diabetic wounds.

Sea Buckthorn Pretreatment, Drying, and Processing of High-Quality Products: Current Status and Trends.

Sea buckthorn is a kind of berry rich in nutritional and industrial value. Due to its thin skin, juicy pulp, and short shelf life, it is usually preserved via freezing methods or directly processed into sea buckthorn puree after harvest. It can also be dried and processed into products such as dried sea buckthorn fruit, freeze-dried sea buckthorn powder, and sea buckthorn oil. This review, therefore, provides an overview of the existing state of drying and high-quality processing of sea buckthorn. The effects of different pretreatment and drying techniques on the drying characteristics and quality of sea buckthorn and the existing problems of superior-quality processing of sea buckthorn products are summarised. The development trend of sea buckthorn drying methods and the ways to achieve high-quality processing of sea buckthorn products are indicated. These ways are mainly related to the following: (1) The application of combined pretreatment and drying techniques to find a balance between economy, ecology, and efficiency; (2) Introducing new online measurement and control technology into drying equipment; (3) Optimising the existing process to form a complete sea buckthorn industrial chain and develop the sea buckthorn deep-processing industry.

Bioconjugation of Vegetable Oils with UV Absorbers: New Approach in Skin Photoprotection.

We reported the tunable synthesis of new vegetable oil-UV filter bioconjugates using sea buckthorn oil (SBO) and p-methoxycinnamic acid (p-MCA) as an alternative to the common UV filter, ethylhexyl-p-methoxycinnamate (octinoxate). The synthetic strategy is based on the sustainable ring-opening reaction of epoxidized SBO with p-MCA in heterogenous catalysis in eco-friendly solvents. The amount of UV-absorptive moieties grafted on the triglyceride backbone is controlled by different epoxidation degrees as determined by NMR spectroscopy. The performance of the new UV-absorber bioconjugates was assessed by in vitro sun protection factor (SPF) measurements after inclusion in SBO-ethylcellulose (EC) oleogels and comparison with the SPF value of the SBO-EC-octinoxate oleogel with equivalent p-MCA acid moieties (10% wt/wt). The concentration obtained for the SBO-EC oleogel formulated with the bioconjugate with the lowest degree of functionalization, namely 55%, represents 45% of the SPF determined for the SBO-EC-octinoxate oleogel, regardless of the concentration of measured solutions. The new concept of vegetable oil-UV-absorber bioconjugates has potential UV-B photoprotective properties when included in oleogel formulations and deserves further investigation of their properties and stability including association with UV-A absorbers, respectively.

Research by choice of excipients ingredients of the gel for the therapy of radiation lesions of the skin based on rheological studies

The aim of this work is to conduct a research analysis of changes in the structural and mechanical properties of the gel from the addition of active substances and surfactants.&#x0D; Materials and methods. In the presented work, model samples of the gel with active substances were studied: freshly obtained stonecrop juice, sea buckthorn oil, rosehip oil, St. John's wort oil and quercetin. As substances for the manufacture of bases hydrocolloids of cellulose derivatives were used: sodium carboxymethylcellulose (NaCMC) and hydroxyethylcellulose (HEC) with a surfactant Lanette SX.&#x0D; A study of the rheological properties of the samples was performed using a rheoviscometer Rheolab QC (Anton Paar, Austria) using a system of coaxial cylinders C-CC27 / SS. The Rheolab QC rheometer is equipped with RheoPlus software, which allows you to set the necessary experimental conditions (shear rate gradient range, number of measuring points and measurement time of one point).&#x0D; Results. The rheological properties of model samples of NaCMC and HEC gels, depending on the used concentration, were studied. With increasing concentrations of both NaCMC and HEC thixotropy of dispersed systems decreases. The change of structural and mechanical parameters of hydrogels from the addition of active ingredients and surfactant is analyzed, and established that adding them into the hydrogels increases the structural and mechanical parameters of model samples.&#x0D; A study of gels with a constant concentration of sodium carboxymethylcellulose and hydroxyethylcellulose and different content of surfactant Lanette SX established that as the surfactant concentration increases, the area of the hysteresis loop and the resistance of the dispersed system to the applied mechanical fracture increase.&#x0D; Conclusions. According to the results of the obtained structural and mechanical parameters and rheograms of the studied model samples of gels, it is rational to use in further studies of sodium carboxymethyl-cellulose (1.5 %) and hydroxyethylcellulose (1.25 %) stabilized Lannete SX in concentrations of 4-5 % and 3-5 % respectively

Non-targeted Profiling of Sea Buckthorn Fruit Oil Fingerprints from 3 Regions and Study on Its Antioxidant Activity.

This study investigated the chemical and volatile characteristics of sea buckthorn fruits from three different regions in China. The chemical composition of the volatile oil was determined by using a non-targeted gas chromatography and mass spectrometry (GC/MS) method and the differences in chemical composition among the three producing areas were compared by heatmap providing a visual basis for researchers. A total of 93 compounds were identified, including 52 compounds from the Northeast China, 51 from the Xinjiang region, and 37 from Inner Mongolia region. Then, the in vitro antioxidant activity of sea buckthorn fruit oil was measured using DPPH, ABTS, and SOD inhibition tests, and the results showed that sea buckthorn fruit oil in northeast China was the strongest antioxidant, followed by Inner Mongolia and Xinjiang. The results of the CCK-8 experiment indicated that within the tested concentration, there is no cell cytotoxicity of the essential oil in human umbilical vein endothelial cells (HUVECs) cells. The results could supply reference to distinguish sea buckthorn fruit from different production areas and, meanwhile, clarify the activity and safety of sea buckthorn oil.

The Composition of Fatty Acids in Bee Pollen, Royal Jelly, Buckthorn Oil and Their Mixtures with Pollen Preserved for Storage.

Honey produced by A. mellifera contains minor components present in the nectar collected from plants. Various studies of honey components and all other bee products can be informative in assessing their quality. The aim of the present study was to determine the content and composition of fatty acids (FAs) in sea buckthorn oil (SBO), royal jelly (RJ) and bee pollen (BP) and the changes in FAs content in these products during storage. The diversity of FAs and the effect of storage time on FAs content was also evaluated for the prepared-for-preservation mixtures, which included the following samples: pollen mixed with honey at a ratio of 1:2 (w/w); sample BPH, a well; BPH + 1% (w/w) SBO; and BPH + 1% (w/w) SBO + 2% (w/w) RJ. Fresh bee-collected pollen and RJ were stored at -20 °C, whereas the conserved samples were stored at +4 °C in hermetically sealed jars. The data revealed that RJ demonstrated the highest diversity of fatty acids compared to BP and BP prepared for storage with honey along with SBO and RJ. Palmitic and stearic acids were found in the highest amounts out of the eight saturated fatty acids identified in the studied SBO and RJ. The amount of these fatty acids in RJ compared to SBO was 1.27 and 6.14 times higher, respectively. In total, twenty-two unsaturated fatty acids (USFA) were identified in RJ and fourteen were found in SBO. The SBO used in this study was found to be high in linoleic acid, resulting in an increased n-6 fatty acids ratio in the prepared samples. Essential fatty acids eicosapentaenoic (EPA) and docosahexaenoic (DHA) were found in RJ, as well as in BP and BP mixed with honey. These FAs were not identified in the samples prepared with SBO even in the sample supplemented with RJ. The highest decrease in docosadienoic fatty acid was found in the BPH sample compared to BP, while arachidonic acid mostly decreased in BPH + 1% SBO compared to the BPH + 1% (w/w) SBO + 2% (w/w) RJ samples stored at +4 °C. Bee-collected pollen had the greatest influence on the number of FAs in its mixture with honey.

Получение облепихового масла экстракцией жома плодов облепихи сверхкритическим диоксидом углерода с последующим центрифугированием

A simple method for production of buckthorn buckthorn oil (Hippophae rhamnoides) with a total carotenoid content sufficient to meet the pharmacopoeial requirements for sea buckthorn oil used as a wound healing agent is proposed. The method is based on the extraction of dry pulp of sea buckthorn fruit with supercritical carbon dioxide at high pressures (50 MPa) to achieve a high content of carotenoids in the oil, followed by centrifugation to separate ballast solid wax fractions. Chromatographic and photometric analysis of the chemical composition of the oil confirm the prospects of this approach to obtaining sea buckthorn oil.

Comprehensive untargeted lipidomic analysis of sea buckthorn using UHPLC-HR-AM/MS/MS combined with principal component analysis

Sea buckthorn is an important ecological and economic plant which has multiple bioactivities. The fruits and seeds of sea buckthorn are rich in oil. However, there are few studies on the differences of lipid profiles of sea buckthorn varieties. Herein, the lipidomic fingerprints of sea buckthorn was established. First, a mixture solvent of methanol and chloroform (2:1, v/v) was selected to extract the lipid of the flesh and seed of sea buckthorn. Then, global lipidomic analysis of different varieties of sea buckthorn was conducted. A total of 16 lipid classes and 112 lipid molecular species were determined. Several molecular species, such as PE (phosphatidylethanolamine) 18:1/18:3, PE18:0/18:1, PE18:0/18:2, etc. were selected as the potential biomarkers to classify the samples. Our study provides a scientific basis for quality control of sea buckthorn and promotes the development of sea buckthorn oil.