Culture Screening Research Articles

Prevention of possible vancomycin resistant enterococcus outbreak caused by infants transferred due to earthquake-trauma

Infection control measures are disrupted due to the large number of patients admitted in a very short time, in the early post-earthquake period. Inter-hospital or intra-hospital transfers can cause the spread of resistant bacteria. We reported the possible outbreak and its prevention caused by vancomycin-resistant enterococcus (VRE) strains carried by infants transferred from another unit to our neonatal intensive care unit after the earthquake. Screening cultures (rectal swab) were obtained from infants on admission and patients were kept in contact isolation. We studied the clonal analysis of VRE strains isolated from patients by Pulse Field Gel Electroforesis.

Physiological liver microtissue 384-well microplate system for preclinical hepatotoxicity assessment of therapeutic small molecule drugs.

Hepatotoxicity can lead to the discontinuation of approved or investigational drugs. The evaluation of the potential hepatoxicity of drugs in development is challenging because current models assessing this adverse effect are not always predictive of the outcome in human beings. Cell lines are routinely used for early hepatotoxicity screening, but to improve the detection of potential hepatotoxicity, in vitro models that better reflect liver morphology and function are needed. One such promising model is human liver microtissues. These are spheroids made of primary human parenchymal and nonparenchymal liver cells, which are amenable to high throughput screening. To test the predictivity of this model, the cytotoxicity of 152 FDA (US Food & Drug Administration)-approved small molecule drugs was measured as per changes in ATP content in human liver microtissues incubated in 384-well microplates. The results were analyzed with respect to drug label information, drug-induced liver injury (DILI) concern class, and drug class. The threshold IC50ATP-to-Cmax ratio of 176 was used to discriminate between safe and hepatotoxic drugs. "vMost-DILI-concern" drugs were detected with a sensitivity of 72% and a specificity of 89%, and "vMost-DILI-concern" drugs affecting the nervous system were detected with a sensitivity of 92% and a specificity of 91%. The robustness and relevance of this evaluation were assessed using a 5-fold cross-validation. The good predictivity, together with the in vivo-like morphology of the liver microtissues and scalability to a 384-well microplate, makes this method a promising and practical in vitro alternative to 2D cell line cultures for the early hepatotoxicity screening of drug candidates.

Cumulative incidence of vancomycin-resistant Enterococcus faecium detection by patient characteristics or possible exposures: prioritization of patients for active screening culture

The target population for active surveillance culture (ASC) of vancomycin-resistant Enterococcus species (VRE) by stool or rectal swabs has not been fully determined during VRE outbreaks in healthcare settings in non-VRE endemic situation. We evaluated cumulative incidences of VRE detection during a vancomycin-resistant Enterococcus faecium outbreak to determine reasonable target populations for ASC. Cases included inpatients whose first VRE-positive sample was obtained at Shizuoka General Hospital between February 2022 and January 2023, during which we conducted admission screening for possible high-risk patients, biweekly screening of all inpatients, admission and discharge screening in the high-care unit, and screening of contacts in each ward by using stool or rectal samples. We calculated cumulative incidences of VRE detection for those screened by patient characteristics or possible exposure. Among 60 cases identified, 55 (92%) were by ASC. Cumulative incidence was higher for contacts (6.4%, 15/234) than for those identified by other screening methods (0.5%, 40/8565). Among the patients identified through admission screening, those previously hospitalized in areas of reported VRE outbreaks had the highest cumulative incidence (6.6%, 5/78) followed by patients requiring toilet assistance (3.7%, 6/161). A bundle approach including ASC and prompt contact precautions by the hospital infection control team, local public health centre, and local and national infection control experts helped terminate the outbreak in seven months. Patients with contacts, prior hospitalization in areas with known VRE outbreaks, and who need toilet assistance appear to be high-risk populations for VRE detection and are candidates for ASC.

Outbreak caused by multidrug-resistant OXA-48 and NDM-1 producing Klebsiella pneumoniae inthe intensive care unit of a cancer hospital.

We report a nosocomial outbreak caused by a multidrug-resistant carbapenemase-producing Klebsiella pneumoniae (MDRCPKp), that was detected in six patients admitted to the medical intensive care unit between 20th of December 2023 and 15th of January 2024 in Ankara, Turkey. The investigation of this outbreak was started on 29th of December 2023. During the outbreak 11 samples were collected from the six patients with MDRCPKp. Pulsed-field gel electrophoresis (PFGE) was performed to determine the genetic relatedness and clonality of MDRCPKp strains. MDRCPKp was isolated in the tracheal aspiration culture, blood, urine, and screening samples. Five patients with MDRCPKp colonization developed healthcare-associated infection. In one patient MDRCPKp was isolated from tracheal aspirate and the screening cultures were considered as colonization not infection. PFGE analysis revealed that all isolates belonged to the same K. pneumoniae clone. MDRCPKp strain of this outbreak exhibited multidrug resistance and co-produced OXA-48 and NDM-1. This outbreak ended after application of strict infection control measures. An outbreak of MDRCPKp can occur in hospitals, especially in the intensive care units; thus, it should be detected early by infection control teams. A strong collaboration between infection control team and microbiology laboratory is essential to cope with MDR bacterial outbreaks in hospitals.

Multiplexed real-time PCR for the detection and differentiation of Klebsiella pneumoniae O-antigen serotypes.

Klebsiella pneumoniae has emerged as a global health threat due to its role in the spread of antimicrobial resistance and because it is a frequent cause of hospital-acquired infections and neonatal sepsis. Capsular and lipopolysaccharide (LPS) O-antigen polysaccharide surface antigens are major immunogens that are useful for strain classification and are candidates for vaccine development. We have developed real-time PCR reagents for molecular serotyping, subtyping, and quantitation of the most prevalent LPS O-antigen types (i.e., O1, O2, O3, and O5) of Klebsiella pneumoniae. We describe two applications for this O-typing assay: for screening culture isolates and for direct typing of Klebsiella pneumoniae present in stool samples. We find 100% concordance between the results of the O-typing assay and whole-genome sequencing of 81 culture isolates, and >90% agreement in O-typing performed directly on specimens of human stool, with disagreement arising primarily from a lack of sensitivity of the culture-based comparator method. Additionally, we find evidence for mixed O-type populations at varying levels of abundance in direct tests of stool from a hospitalized patient population. Taken together, these results demonstrate that this novel O-typing assay can be a useful tool for K. pneumoniae epidemiologic and vaccine studies.IMPORTANCEKlebsiella pneumoniae is an important opportunistic pathogen. The gastrointestinal (GI) tract is the primary reservoir of K. pneumoniae in humans, and GI carriage is believed to be a prerequisite for invasive infection. Knowledge about the dynamics and duration of GI carriage has been hampered by the lack of tools suitable for detection and strain discrimination. Real-time PCR is particularly suited to the higher-throughput workflows used in population-based studies, which are needed to improve our understanding of carriage dynamics and the factors influencing K. pneumoniae colonization.

The cloth pass and soft glitch

In this article, sound artist Julie Rose Bower examines the relationship between the Foley ‘cloth pass’ and the ‘fabric sounds’ autonomous sensory meridian response (ASMR) trigger. Starting with Serres’s sensory appraisal that ‘the world is a mass of laundry’ the tactile qualities of cloth are proposed as a key component in accounting for the ‘felt rather than heard’ status of Foley sound. Writing from a practitioner perspective, Bower argues for textile manipulation as a form of embodied sound design and cloth sounds as a curiously generative media object. Through theorizing developments in the scope of attention within screen cultures, Bower develops a speculative ontology of soft glitch and conceptualizes a politics of folded listening. Alongside accounts of sound practice from industry Foley artists Vanessa Theme Ament (Die Hard, Edward Scissorhands), Joanna Fang (Sony PlayStation) and from her own ASMR work using costume and textiles (ASMR at the Museum for the Victoria and Albert Museum [V&A]), Bower argues for cloth sounds as transcendent cyberfeminist technique.

Формирование «новой зрелищности». Как устроен современный стриминг (на примере Netflix и др.)

The paper aims to identify the characteristics of the “new spectacle” in the context of screen digitization. The author’s focus on identifying the stages of screen digitization and the emergence of new cultural practices when viewing content on streaming services is crucial. The scientific novelty of the study lies in its multifaceted analysis of media consumption and media production transformations in the digital age, particularly emphasizing the significant role of streaming services in these processes. The author investigates the interactive aspects of streaming services where viewers can influence the plot, which is a significant contribution to understanding contemporary media practices. As a result, the author emphasizes that streaming services have become catalysts for numerous changes in the media industry, leading to democratization of content access and production. The work notes the possibility of a functional differentiation of modern media consumption culture under the influence of screen culture before the streaming era and during the streaming era. The emergence of new demands for content creation in the context of the “new spectacle” is highlighted.

Prospective Analysis of urINe LAM to Eliminate NTM Sputum Screening (PAINLESS) study: Rationale and trial design for testing urine lipoarabinomannan as a marker of NTM lung infection in cystic fibrosis.

Routine screening for nontuberculous mycobacterial (NTM) lung disease is dependent on sputum cultures. This is particularly challenging in the cystic fibrosis (CF) population due to reduced sputum production and low culture sensitivity. Biomarkers of infection that do not rely on sputum may lead to earlier diagnosis, but validation trials require a unique prospective design. The rationale of this trial is to investigate the utility of urine lipoarabinomannan (LAM) as a test to identify people with CF with a new positive NTM culture. We hypothesize that urine LAM is a sensitive, non-invasive screening test with a high negative predictive value to identify individuals with a relatively low risk of having positive NTM sputum culture. This is a prospective, single-center, non-randomized observational study in adults with CF, 3 years of negative NTM cultures, and no known history of NTM positive cultures. Patients are followed for two year-long observational periods with the primary endpoint being a positive NTM sputum culture within a year of a positive urine LAM result and a secondary endpoint of a positive NTM sputum culture within 3 years of a positive urine LAM result. Study implementation includes remote consent and sample collection to accommodate changes from the COVID-19 pandemic. This report describes the study design of an observational study aimed at using a urine biomarker to assist in the diagnosis of NTM lung infection in pwCF. If successful, urine LAM could be used as an adjunct to traditional sputum cultures for routine NTM screening.

Active follow-up of patients identified with multidrug-resistant Gram-negative bacteria to discontinue contact precautions and isolation measures

It is essential to refrain from unnecessary isolation measures indicated for patients identified with multidrug-resistant Gram-negative bacteria (MDR-GNB). To evaluate whether a pro-active follow-up strategy to discontinue isolation measures of patients identified with MDR-GNB (without carbapenemase production) resulted in reduced isolation days during hospitalization, compared to passive follow-up. A comparison was made between active and passive follow-up strategies over a two-year period after first MDR-GNB identification. Patients could be declared negative after two consecutive negative screening cultures. Active follow-up patients received a questionnaire for screening cultures within six months of MDR-GNB identification. Of the 2208 patients included, 1424 patients (64.5%) underwent passive follow-up and 784 patients (35.5%) underwent active follow-up. A significantly higher proportion of active follow-up patients who had sufficient (at least two) screening cultures were declared MDR-GNB negative compared to those with passive follow-up; 66.9% vs 20.6% (P < 0.001) for adult patients and 76.0% vs 17.1% (P < 0.001) for paediatric patients. A comparison between active follow-up patients with sufficient versus those with active follow-up but insufficient cultures revealed a reduction of isolation days for paediatric patients (median 10.6 vs 1.6 days; P= 0.031). Though this difference was not statistically significant for adults (median 5.3 vs 4.2 isolation days), there was a valuable decrease in the number of isolation days for both adult and paediatric patients under active follow-up with sufficient (≥2) cultures, indicating clinical relevance. We recommend an active follow-up strategy for patients identified with an MDR-GNB, to prevent further unneeded infection prevention measures.

Millennial Screen Cultures in Spain: Queering the Mainstream*

This article interrogates queer representations in twenty-first century Spanish film and digital media, focusing on a group of emerging millennial filmmakers: Paco León, Eduardo Casanova, and the duo Javier Ambrossi and Javier Calvo (‘Los Javis’). It argues that films and digital series by these directors are representative of a Spanish queer aesthetics which navigates between combative subversion and mainstream inclusion. It assesses to what extent contemporary screen cultures are still challenging hegemonic social and ideological constructs in relation to gender, sexuality and identity and in what ways they grapple with LGBTQ + visibility and subjectivity vis-à-vis the mainstream audio-visual media industry.

Large-Scale S. aureus Screening with Molecular Epidemiology; the Role of MSSA and Community MRSA in Hospital Transmissions

Background: The frequency of Staphylococcus aureus transmission in hospitals is unknown: symptomatic infection may occur months after transmission and colonization, and infection prevention efforts rely on indirect measurements, rather than direct detection of transmission events. We implemented a hospital-based S. aureus screening program, combined with whole genome sequencing of S. aureus surveillance and clinical cultures and data extracted from the electronic health record, to identify S. aureus clonal complex-, patient- and location-specific factors associated with S. aureus transmission in our health system. Methods: Screening S. aureus cultures were obtained at admission by nasal swab for adults admitted to Medicine, Transplant, Oncology and intensive care, and weekly by swab of nares, axilla and groin for children admitted to intensive care and Oncology at NYU Langone Health in New York City. All methicillin-resistant S. aureus (MRSA) from screening and clinical (blood, wound, sputum) cultures and all methicillin-susceptible S. aureus (MSSA) from screening and blood cultures underwent whole genome sequencing. Isolates from distinct patients with &lt; 2 0 single nucleotide pair differences were considered genetically related. Electronic health data was extracted for descriptive statistics and for spatiotemporal plots to assess plausible transmissions. We used REDCap electronic data capture tools hosted at NYU Grossman School of Medicine and SAS software for data analysis to evaluate S. aureus transmissions between November 2022 and November 2023. Results: We analyzed 8,567 S. aureus isolates: including 6,552 screening cultures, 1,008 blood cultures, and 1,007 clinical cultures. We found 424 plausible S. aureus hospital transmissions using sequencing and electronic health data. Screening cultures identified 75% of transmissions that would have otherwise been missed with blood and clinical cultures alone. The majority of positive screening cultures isolated MSSA, but the proportion of transmissions due to MSSA differed by age. In children, MSSA colonization accounted for 62% of transmissions. In adults, only 15% of transmissions were due to MSSA colonization, whereas MRSA colonization accounted for 56% of transmissions. Analysis of adult MRSA isolates by clonal complex found that 45% of transmissions were due to CC8, higher than the 17% among isolates agnostic of transmissions. Emergency departments and the neonatal intensive care unit had the highest number of transmissions. Patients involved in transmissions had longer lengths of stay and frequent hospitalizations. Conclusions: A S. aureus screening program, coupled with genome sequencing and electronic health data, can identify patient group, hospital locations and clonal complexes that are at high risk for S. aureus transmissions.

Exploring treatment and antifungal resistance in an outbreak of tinea caused by Microsporum audouinii

AbstractBackgroundMicrosporum audouinii has resurged recently. Infections with the dermatophyte are difficult to treat, which raises the question if we treat M. audouinii infections with the most effective antifungal (AF) agent.ObjectivesThe aims of this study was to investigate an outbreak of tinea capitis (TC) in Denmark, address the challenges in outbreak management and to conduct two reviews regarding previous outbreaks and minimal inhibitory concentration (MIC).MethodsWe used Wood's light, culture, direct microscopy, and PCR for screening and antifungal susceptibility testing (AFST) for treatment optimization. We performed two reviews to explore M. audouinii outbreaks and MIC values using broth microdilution method.ResultsOf 73 screened individuals, 10 had confirmed M. audouinii infections. Clinical resistance to griseofulvin was observed in 4 (66%) cases. While previous outbreaks showed high griseofulvin efficacy, our study favoured terbinafine, fluconazole and itraconazole in our hard‐to‐treat cases. AFST guided the choice of AF. Through the literature search, we identified five M. audouinii outbreaks, where differences in management included the use of Wood's light and prophylactic topical AF therapy. Terbinafine MIC values from the literature ranged from 0.002 to 0.125 mg/L.ConclusionUse of Wood's light and preventive measurements were important for limiting infection. The literature lacked MIC data for griseofulvin against M. audouinii, but indicated sensitivity for terbinafine. The clinical efficacy for M. audouinii treatment was contradictory favouring both terbinafine and griseofulvin. AFST could have a key role in the treatment of difficult cases, but lack of standardisation of AFST and MIC breakpoints limits its usefulness.

Formation of national and cultural identity as a function of spectacularity and spectacularity culturee

“Spectacularity” in the modern information society is one of the crucial factors and tools for the formation of national and cultural identity, since its role in festive, ceremonial, visual, television, screen, Internet culture, and other similar types of postmodern culture, which have the most important ideological and educational resources for the formation of national and cultural self-awareness and self-identifi cation of citizens in society, is paramount. In modern times, namely in the era of postmodernism, the screen, as well as screen, television, Internet, and visual culture are a powerful ideological weapon, an infl uential educational tool, participating in the creative national and cultural policy of the state. The materials of the article can be used in the educational process in the Cultural Studies major.

Challenging the Mainstream Portrayal of Islam in Indonesian Screen Culture: Religious Representation in Hati Suhita Film

This article examines the representation of Islam in Indonesian screen culture, focusing on the genre of film Islami. It discusses the dominant portrayal of muslims piety and modernity in these films and explores the smaller number of films that depict pesantren culture, a manifestation of traditional Islam. The article analyzes the Hati Suhita film as a significant shift in representing a solid traditional Islam rooted in history, culture, and the modern values to shape religiosity. It highlights the film's exploration of a love triangle within an arranged marriage in a non-cliché manner through strength and resilience, grounded on Javanese philosophy and sufism, while promoting a gender justice perspective. It also discusses the film's impact on the pesantren community, and its role in becoming a convergence point between pesantren and screen culture, two domains that have been considered distant from each other. This article also observes that the success of this film is the result of a long endeavor to negotiate pesantren with popular culture. Overall, the article contributes to the ongoing discourse on the representation of Islam in Indonesian screen culture, and the intersection of tradition, modernity, and religion.

Staphylococcus aureus carriage and prevalence of skin and soft tissue infections among people who inject drugs: a longitudinal study

People who inject drugs are frequently colonized with Staphylococcus aureus and have an increased risk for skin and soft tissue infections. This longitudinal study aims to describe S. aureus carriage in this group and the risk for infections during a 1-year follow-up. We included 61 participants from the Malmö Needle Exchange Program. Mapping of S. aureus carriage was conducted by screening cultures every third month and S. aureus growth was semi-quantified. Data regarding infections and living conditions were collected from structured interviews. Statistics included univariate analysis with the Fischer’s exact test, univariate logistic regression and multivariate logistic regression. S. aureus carriage was detected in 46–63% of participants, and 75% reported one or more infections during the study period. Self-reported infections were associated with carriage in perineum (OR 5.08 [95% CI 1.45–17.73]), in skin lesions (OR 1.48 [95% CI 1.21–1.81]), and unstable housing situation (OR 12.83 [95% CI 1.56–105.81]). Thus, people who inject drugs are frequent carriers of S. aureus and report a surprisingly high prevalence of skin and soft tissue infections. Homeless people and those with skin carriage seem to be at highest risk. Effective clinical interventions are needed, aiming at preventing infections in this vulnerable group.

Clinical impact of active screening cultures for carbapenem-resistant Acinetobacter baumannii: A systematic review and meta-analysis

Carbapenem-resistant Acinetobacter baumannii (CRAB) has emerged as a significant health care-associated infection carrying substantial mortality. We assessed the clinical impact of active screening cultures for CRAB. A systematic review and meta-analysis, aiming to answer 2 questions: (1) Does screening versus no screening improve clinical outcomes? (2) Does positive screening ("CRAB carrier") predict CRAB infections? We searched the literature until January 2024 for comparative studies reporting clinical outcomes (mortality, invasive CRAB infections). Of 5,407 screened publications, 9 studies (10,865 individuals) were included. Invasive CRAB infection rate was significantly higher among CRAB carriers (OR 11.14, 95% CI 4.95-25.05, with substantial heterogeneity stemming from size rather than direction of the effect). Negative predictive value of noncarriage for invasive infection was 97%. CRAB bloodstream infectionrate was significantly higher among carriers (odds ratio 16.23, 95% confidence interval 2.9-110.08). No difference was demonstrated between the groups forCRAB ventilator-associated pneumonia, length of stay, and mortality. Only 1 study reported outcomes for study question #1. Data to support active CRAB screening are scarce regarding its clinical benefit for patients. Positively screened patients are at significantly higher risk for invasive CRAB infections, with high negative predictive value for noncarriage. This did not translate to reduced mortality.

РЕПРЕЗЕНТАЦИЯ ПОСТЧЕЛОВЕЧЕСКОЙ СУБЪЕКТИВНОСТИ В СОВРЕМЕННОЙ ФАНТАСТИКЕ НА ПРИМЕРЕ СЕРИАЛА «ЛЮБОВЬ. СМЕРТЬ. РОБОТЫ»

Today, the conversation about the impact of technology on humans is a common place. In humanitarian theory, there is a growing number of approaches that develop new ways to describe a person in a technologized culture. Special attention is paid to it by posthumanism, based on the experience of critical analysis of culture, it offers a description of alternative, posthuman subjectivity. Where is also a reflection on changing human relations with the environment in visual art. Although mass screen culture has transformed and moved away from big screens to gadgets for private viewing, it has not lost its ability to respond sensitively to cultural trends and offers examples of images of posthuman subjectivity. The theory of posthumanism turns out to be a relevant tool for analyzing posthuman images in modern science fiction films. The article will examine representations of posthuman subjectivity in the genre of modern fiction on the example of the TV series “Love. Death. Robots.” The focus of the analysis will be on the images of the main characters and the peculiarities of plot development in relation to traditional cultural constructs.

Asymmetry of Screen and Book Culture

The digital tsunami, the invasion of which was unimaginable just a generation ago, raises numerous questions among philosophers, cultural scientists, and specialists in other professions. Some of these questions run as follows: is the transition from book culture to screen culture irreversible; are there intermediate stages of this transition; and, why is there no reverse development of the book from the film? Answers are offered in this article. The accepted view is that the standard of book culture is literature (presumably a novel), and in particular, philosophical treatises; and the genuine “screen” paragon is cinema (a film), and a play on television, as a mediator. Other forms of cultural phenomena, events and facts are also discussed, which are associated with the indicated invasion of “digitals” into the life of society and of the individual, causing a restructuring of private and public consciousness.

Evaluating the Clinical Relevance of Routine Sonication for Periprosthetic Hip or Knee Joint Infection Diagnosis.

Periprosthetic joint infection (PJI) is a serious complication after joint arthroplasty. PJI screening and conventional cultures may be inconclusive. Sonication fluid culturing stands out as a valuable adjunct technique for PJI diagnosis. This study aims to determine the clinical relevance of routine sonication for all (a)septic revisions. All patients who underwent (partial) hip or knee revision arthroplasty between 2012 and 2021 were retrospectively reviewed. We formed three groups based on the European Bone and Joint Society PJI criteria: infection confirmed, likely, and unlikely. We analyzed clinical, laboratory, and radiological screening. Sensitivity and specificity were calculated for synovial fluid (preoperative), tissue, and sonication fluid cultures. We determined the clinical relevance of sonication as the percentage of patients for whom sonication confirmed PJI; 429 patients who underwent (partial) revision of hip or knee arthroplasty were included. Sensitivity and specificity were 69% and 99% for synovial fluid cultures, 76% and 92% for tissue cultures, and 80% and 89% for sonication fluid cultures, respectively. Sonication fluid cultures improved tissue culture sensitivity and specificity to 83% and 99%, respectively. In 11% of PJIs, sonication fluid cultures were decisive for diagnosis. This is applicable to acute and chronic infections. Sonication fluid cultures enhanced the sensitivity and specificity of PJI diagnostics. In 11% of PJI cases, causative pathogens were confirmed by sonication fluid culture results. Sonication fluid culture should be performed in all revision arthroplasties.

The Work of Care

This essay examines feminist curation as a work of care with the example of Purplay, a South Korean streaming service. In less than four years since its official launch, Purplay has grown into a platform that currently streams 330 films made by women, hosts a community of 30,000 subscribers, and produces new discourse on women’s cinema through its various initiatives. I analyze an array of Purplay’s curatorial practices as a feminist intervention that not only provides increased access to women’s films but also forges a sense of community among the platform’s participants. These practices, I argue, have constituted a sober reckoning with the inequitable dynamics that characterize South Korea’s current social fabric, and are redefining its screen culture.