Huanglongbing (HLB) disease is a threat to most citrus (Citrus sp.) producing areas and is associated with the bacterium Candidatus Liberibacter asiaticus. The disease is transmitted by the vector asian citrus psyllid [ACP (Diaphoria citri)]. Antipsyllid screen houses can potentially reduce and eliminate HLB development in young citrus plantings by excluding the insect vector. These structures are also anticipated to represent a new environmental platform to cultivate high-valued fresh citrus. The purpose of this investigation was to evaluate the effect of screen houses on excluding infective ACP from inoculating grapefruit (Citrus ×paradisi) trees and determine changes on environmental conditions caused by the screen cloth. We tested two coverings [enclosed screen house and open-air (control)] and two planting systems (in-ground and container-grown), with four replications arranged in a split-plot experimental design. Psyllid counting and HLB diagnosis were performed monthly, and the antipsyllid screen excluded the HLB vector from the houses. ACP and HLB-positive trees were found only at the open-air plots. Weather monitoring was performed every 30 minutes from 22 Feb. to 31 July 2014. Solar radiation accumulation averaged 6.7 W·m−2·minute−1 inside the screen houses and 8.6 W·m−2·minute−1 in the open-air. Air temperature was greater inside the screen houses whereas wind gusts were higher in the open-air. Reference evapotranspiration accumulation averaged 3.2 mm·day−1 inside the screen houses and 4.2 mm·day−1 in the open-air. There was no difference in cumulative rainfall between screen houses and open-air. The antipsyllid screen houses reduced solar radiation, maximum wind gust, and reference evapotranspiration (ETo). The environmental conditions inside the protective screen houses are suitable for grapefruit production.