Objective: Arterial hypertension is a major cardiovascular risk factor that contributes to cardiovascular diseases and associated target organ injuries. World Health Organization estimated that hypertension affected around 1.28 billion adults aged between 30 to 79 years worldwide making it a prime cause of premature death. Design: Observational study Methods: The present study compared the blood derived extracellular vesicles from the American Heart Association guidelines classified study subjects as normal elevated stage 1 stage 2 and hypertensive crisis. EVs were isolated and characterized for surface tetraspanin expression marker CD 41 using flow cytometer and nano tracking analysis Total protein from each sample was extracted and mass spectrometry was performed. Results: Study subjects with mean age of 57 years with arterial hypertension more prevalent in women 62 percent compared to men 38 percent. Positive expressions of tetraspanin CD41 were found in EVs isolated from all patients of each group. Nanotracking analysis of isolated EVs showed an average size of 167 nm with no comparable differences among study groups. Significant differences were found in the mass spectrometric analysis within the study groups in 28 proteins compared using ANOVA with the Kruskall Wallis test followed by Dunns test. Conclusion: The results of the study concluded that proteomic analysis of isolated proteins within the study groups can be explored as diagnostic biomarkers for screening of arterial hypertension and these specific proteins can be further explored for fabricating point of care devices for a community screening of arterial hypertension