Objective: To evaluate the intervention effects of seated Baduanjin exercise on patients undergoing maintenance hemodialysis (MHD). Methods: A total of 108 MHD patients admitted between July 2022 and July 2023 were selected. They were randomly assigned into two groups: the experimental group, with 55 patients, who received seated Baduanjin exercise combined with leg exercises during dialysis; and the control group, with 53 patients, who only performed leg exercises during dialysis. The psychological state, fatigue symptoms, quality of life, and sleep quality scores were compared between the two groups, and exercise endurance was recorded. Results: After the intervention, the psychological state scores in the experimental group were lower than those in the control group, the fatigue symptom scores were lower, the quality of life scores were higher, the sleep quality scores were lower, and the exercise endurance was higher (P < 0.05). Conclusion: Seated Baduanjin exercise can improve the negative psychological state and fatigue symptoms of MHD patients, enhance their quality of life and sleep quality, and effectively increase their exercise endurance.