Scientific Research Research Articles

Morphological characteristics of talocalcaneal coalitions: Anatomical observation, finite element analysis and clinical application

BackgroundThe intricate anatomical structure of talocalcaneal coalitions (TCCs) presents significant challenges for clinicians in both diagnosis and treatment. This study aimed to investigate the anatomy, imaging characteristics, and biomechanical properties of TCCs, providing essential references for contemporary clinical diagnosis, treatment, scientific research, and education regarding TCCs. MethodsThe morphologies of TCCs were examined and classified in intact dry osseous specimens from 131 Asian adults. The imaging characteristics of TCCs were summarized by carefully observing the X-rays and CT scans. Additionally, finite element models of TCCs were established and validated, allowing for the simulation and analysis of stress and strain. ResultsThe TCCs were primarily located in the region between the posterior end of sustentaculum tali (ST), the medial port of the tarsal canal, and the medial edge of the posterior talar articular surface (PTF). In comparison to specimens with cartilage and ligament connections, the medial tubercle of osseous connections exhibited significant inward and downward protrusion, while the ST was longer and thicker. Statistically significant differences were noted in the widths of the calcaneus and talus, as well as in the thickness and length of the ST (P < 0.05). CT imaging provided an accurate determination of TCCs’ locations, while X-rays revealed the presence of the “C sign” and “duck beak sign” in all osseous connection specimens. Finite element model analysis indicated that stress was primarily concentrated at the osseous connection, which also reduced displacement of the subtalar joint. ConclusionsThe comprehension of the precise location, anatomical morphological characteristics, imaging features and finite element mechanical properties of TCCs is instrumental in enhancing the diagnosis and treatment of TCCs.

Coupling coordination analysis and spatiotemporal heterogeneity between urban land green use efficiency and ecosystem services in Yangtze River Economic Belt, China

Rapid urbanization has resulted in the conversion of different land types, leading to a serious disruption of the balance of ecosystems, and the contradiction between land use and ecological security becomes increasingly severe. As a key factor affecting ecosystem services (ESs), improving the urban land green use efficiency (ULGUE) can effectively mitigate the decline in the ecosystem services function. This study tried to verify the profound impact of land use on ESs by measuring the coupling coordination degree (CCD) between ULGUE and ESs from the perspective of coupling coordination. However, the traditional CCD model suffers from volatility and non-comparable confidence levels in results. For this background, this study adopted the improved CCD model to measure the association between ULGUE and ESs in the Yangtze River Economic Belt (YREB). Also, a comprehensive spatial evolution of the CCD was further explored. The main conclusions are as follows: (a) The overall level of ULGUE in YREB showed an increasing trend with the distribution of ULGUE in most cities ranging from 0.71 to 1.00, showing a higher level overall and the spatial distribution character of “small aggregation, large distribution”; (b) The ESs of YREB from 2005 to 2020 demonstrated a decreasing trend and majority of cities’ current ecological protection efforts are lagging behind the extent of ecosystem damage caused by human activities; (c) The overall average CCD of YREB was largely at basically balanced level and the average CCD change ranges from 0.38 to 0.45 between 2005 and 2020, but the overall CCD had a strong spatial correlation strength. (4) To some extent, the improved CCD model solved the problems of volatility and credibility based on the traditional CCD results, which have strong rationality and scientific validity. Identifying the coupling and coordination characteristics and mechanisms can effectively provide theoretical support for the sustainable development of watersheds.

Dreaming conundrum.

Dreaming, a common yet mysterious cognitive phenomenon, is an involuntary process experienced by individuals during sleep. Although the fascination with dreams dates back to ancient times and gained therapeutic significance through psychoanalysis in the early twentieth century, its scientific investigation only gained momentum with the discovery of rapid eye movement (REM) sleep in the 1950s. This review synthesises current research on the neurobiological and psychological aspects of dreaming, including factors influencing dream recall and content, neurophysiological correlates, and experimental models, and discusses the implications for clinical practice.

Masculine Violence in Turkish Folk Dances

As a social issue, violence continues to manifest and remains a current topic of academic research. Scientific studies mostly focus on the individual and societal dimensions of violence; however, studies that address violence in the context of everyday life practices remain limited. This study argues that violence has a masculine character as a result of gender inequality and within the framework of the masculinity-power relationality. On the other hand, this study considers folk dances as a product of social culture and views them as an effective form of non-verbal communication within society. The study is based on the claim that, in folk dances observed in formal or informal ceremonial events, which are part of everyday life and contain elements of violence, violence is legitimized as a spectacle. In this context, local folk dances from different regions of Turkey, performed exclusively by male dancers and portraying themes of heroism, victory, bravery, and courage, have been selected as examples. These include Trabzon Bıçak Horonu, Bursa Kılıç-Kalkan, Manisa Seğmen Sekmesi, Balıkesir Bengi, Erzurum Köroğlu Barı, Giresun Tüfekli Çandır Karşılaması, and Diyarbakır Şur-u Mertal. The violent content of these dances has been analyzed through the method of semiotic analysis. Weapons used in these dances, fast and rhythmic tunes, and rigid movements have been considered as indicators of violence. It has been concluded that these dances, admired and enthusiastically applauded, normalize violence. This study makes a significant contribution by addressing folk dances within the context of violence and aims to fill the gap in the literature in this field.

Optimized protocol for the multi-omics processing of cryopreserved human kidney tissue.

Biobanking of tissue from clinically obtained kidney biopsies for later use with multi-omic and imaging techniques is an inevitable step to overcome the need of disease model systems and towards translational medicine. Hence, collection protocols ensuring integration into daily clinical routines using preservation media not requiring liquid nitrogen but instantly preserving kidney tissue for clinical and scientific analyses are of paramount importance. Thus, we modified a robust single nucleus dissociation protocol for kidney tissue stored snap frozen or in the preservation media RNAlaterand CellCover. Using porcine kidney tissue as surrogate for human kidney tissue, we conducted single nucleus RNA sequencing with the Chromium 10X Genomics platform. The resulting data sets from each storage condition were analyzed to identify any potential variations in transcriptomic profiles. Furthermore, we assessed the suitability of the preservation media for additional analysis techniques (proteomics, metabolomics) and the preservation of tissue architecture for histopathological examination including immunofluorescence staining. In this study, we show that in daily clinical routines the RNAlater facilitates the collection of highly preserved human kidney biopsies and enables further analysis with cutting-edge techniques like single nucleus RNA sequencing, proteomics, and histopathological evaluation. Only metabolome analysis is currently restricted to snap frozen tissue. This work will contribute to build tissue biobanks with well-defined cohorts of the respective kidney disease that can be deeply molecularly characterized, opening new horizons for the identification of unique cells, pathways and biomarkers for the prevention, early identification, and targeted therapy of kidney diseases.

Improving horizon computation algorithm with quasirandom sequences

The topographic horizon can be used to estimate shading for applications requiring an accurate solar resource estimate, including the effects of the local terrain. With high-resolution topography data, horizon estimation significantly contributes to the computation time needed for irradiance simulations. Approximate horizon algorithms have been proposed previously, sampling topography in a finite number of directions. This paper extends this idea by presenting a sampling approach based on the quasirandom sequences. The sampling strategy is optimized with 300,000 km2 of topography data from Europe and the USA. We demonstrate that our algorithm is several orders of magnitude faster than the precise horizon calculation and significantly improves the previous approximate algorithms. In particular, at the ground level with a topography resolution of 1 m/pixel, the proposed method achieved the same accuracy as the old approximate method four times faster. The horizon is one of the key methods in geographical information science and a part of many modeling tools in the built environment. Improvements in computation time benefit all applications that require irradiance modeling, e.g., the yield of vehicle- and building-integrated photovoltaic or temperature modeling of buildings.

The comparatist fervor. Transfers, contagions and influences in history and social sciences

Abstract How do scientific research methods circulate?1 Why do they acquire, at a given moment, such dynamism and fecundity in various disciplines? How did the comparative fervor catch on in the social sciences of the 19th and 20th centuries, resulting in comparative history and global history in the Annales project? The field of transfers through which the comparative method circulated between Victorian anthropology, Durkheimian sociology and the linguistics associated with it and history, through the pen of French-speaking medievalists, explains the dynamism of transmissions and contagions, of crossed, filtered or direct influences. This interplay of exchanges between disciplines, authors and books represents a moment in the history of the comparative method, in which it was considered a paradigm of renewal in the disciplines in which it was practiced, as well as for the social sciences of its time. Within the French methodological tradition, the relations between comparative history and global history are close. The scales of comparison make it possible to characterize civilizations, societies and economies, and to measure their dimensions in different times and spaces. But the latter corresponds to differences of social milieu and historical peculiarities, which are an original innovation, the result of historians’ borrowings from and rejections of other social scientists. In this comparative fervour the games of intellectual exchange defined comparative history as the history of civilizations considered in the long term.

The Methodology of Mas’ud Samarkandi in Solving Jurisprudential Issues

This article discusses the scientific and theoretical sources and methods used by Mas'ud ibn Mahmud ibn Yusuf Samarkandi, who lived at the end of the 13th century and the beginning of the 14th century, in writing his work "Saloti Mas'udi". The article provides a detailed analysis, supported with examples, of his approach to resolving jurisprudential issues.

Fractal Analysis and Fractal Dimension in Materials Chemistry

Using fractal analysis and computing fractal dimension to understand materials chemistry and solve problems such as catalysis, chemical reactions, fuel storage, and material design is an important scientific research domain [...]

Academic freedom: Global variations in norm conceptualization, diffusion and contestation – an introduction

Abstract This Introduction provides an overview of the topics covered in this special issue on ‘Academic freedom: Global variations in norm conceptualization, diffusion and contestation’, which explores what academic freedom means, how this may vary on a global level, how the norm spread around the world and what current contestations look like. The Introduction defines some terms essential to this debate, such as the freedom of science, academic freedom, freedom of scientific research and the right to science, and offers an analytical framework for the various contributions of the special issue. This includes, in particular, a distinction between illiberal and liberal science scripts and their relationship with academic freedom, as well as between different forms of contestations and limitations of academic freedom. Authors from varying disciplinary and regional backgrounds address different aspects of this theme in their respective contributions, of which the introduction gives a brief summary.

Growth-phase-dependent control of rRNA synthesis in Saccharomyces cerevisiae.

Saccharomyces cerevisiae is one of the most well-studied model organisms used in the scientific community. Its ease of manipulation, accessible growth conditions, short life cycle, and conserved eukaryotic metabolic pathways make it a useful model organism. Consequently, yeast has been used to investigate a myriad of phenomena, from microbial to human studies. Most of the research performed using this model organism utilizes yeast cell populations when they are growing exponentially, a growth phase aptly termed exponential or log phase. However, log phase encompasses several yeast generations and ranges several hours of yeast growth, meaning that there is a potential for variability during this "homogenous" growth phase. Cells in log phase require robust ribosome biogenesis to support their rapid growth and cell division. Interestingly, during log phase, ribosomal RNA (rRNA) synthesis (which is the first and rate limiting step in ribosome biosynthesis) has been shown to decrease prior to growth rate decline in stationary phase. In this study, we utilized several genomic and biochemical methods to elucidate the relationship between subphases of log phase and rRNA synthesis. Our results indicate that as yeast cells progress through subphases of log growth, both polymerase I transcription and rRNA processing are repressed. Overall, this study establishes a growth-phase-dependent control of rRNA synthesis that unexpectedly begins prior to the switch to stationary phase (i.e., pre-diauxic shift) as a putative mechanism of anticipating nutrient starvation.IMPORTANCESaccharomyces cerevisiae is a ubiquitously used model organism in a wide range of scientific research fields. The conventional practice when performing yeast studies is to investigate its properties during logarithmic growth phase. This growth phase is defined as the period during which the cell population doubles at regular intervals, and nutrients are not limiting. However, this growth phase lasts hours and encompasses several yeast cell generations which consequently introduce heterogeneity to log growth phase depending on their time of harvest. This study reveals significant changes in the transcriptomic landscape even in early stages of exponential growth. The overall significance of this work is the revelation that even the seemingly homogenous log growth phase is far more diverse than was previously believed.

Echo of extinction: The Ivory-billed Woodpecker's tragic legacy and its impact on scientific integrity

Abstract Among the current surge in extinction rates, exceeding between 100 and 1000 times the prehuman background rate, a multitude of species quietly fade away, largely escaping the notice of our collective awareness. The extinction of only a select few species has the power to captivate public attention, stir emotions, and ignite debates comparable to the case of the ivory-billed woodpecker (Campephilus principalis). Reports purporting a rediscovery of this long-extinct species periodically surface and find their way into peer-reviewed journals, attracting worldwide attention from the public media. This phenomenon persists despite a glaring lack of evidence supporting such an exceptional claim, in disconnect with principles of population biology or even common sense, thereby risking erosion of public trust in the foundations of nature conservancy and the integrity of scientific methodologies in general. The tragic fate of this iconic species therefore holds significance beyond ecology, providing deep insights into extinction and conservation while highlighting the need for refinement of scientific rigor and standards of evidence in the peer review process and beyond.

Scientific Approaches to the Characterization, Processing, Production, and Marketing of Novel Herbal Plants

Scientific Approaches to the Characterization, Processing, Production, and Marketing of Novel Herbal Plants

DevOps Adoption Guidelines, Challenges and Benefits: A Systematic Literature Review

The DevOps methodology in software engineering is currently trending and many software development organisations are adopting it to stay competitive. However, these organisations face difficulties in adopting DevOps due to a lack of common standard guidelines and methodology. We argued that guidelines across multiple platforms are needed to succeed in DevOps implementation. This paper aims to identify the benefits, challenges, and guidelines associated with DevOps adoption using a systematic literature review. The search spanned over ten digital libraries such as ACM Digital Library, ScienceDirect, EBSCOhost, SAGE Journals, Wiley Online Library, and so on. The review also included keyword searches in relevant studies i.e DevOps guidelines, DevOps and guidelines, DevOps benefits, DevOps and benefits, DevOps challenges, DevOps and challenges. Papers written in English and published from 2011 through July 2020 were considered. 33 studies passed the two-step quality assessment. Finally, the data were extracted and analysed based on the themes and research questions, revealing the benefits and challenges of DevOps adoption guidelines. The findings show that improved software delivery speed increases code quality and application quality with frequent releases. It also shows that there was no transparent scientific approach to determining the DevOps capabilities regarding the process, practice, tools, and implementations, cost implications, and insufficient knowledge of the automation process. Finally, the reviewed studies indicated that software quality increases whenever DevOps best practices are adopted. DevOps is about continuous software delivery. Previous studies have not considered the guidelines for scientifically adopting DevOps for organisations that want to migrate. The present review identified the DevOps adoption guideline model represented in a cyclic format that indicates an infinite process representing continuous integration, continuous delivery, and continuous deployment, with an understanding of benefits and challenges.

Boron Salicylate Ester Compounds as Boron Therapeutics. Their Synthesis, Structural Characterizations and Anticancer Effects against MDA-MB-231.

The element boron forms a wide range of borate minerals with different properties. Borate minerals make it possible to design boron-containing molecules with new biological properties in terms of their chemical structure and properties. It is known that boron compounds have antioxidant, anti-inflammatory, anti-tumor and anti-cancer properties. This makes boron compounds important for the future development of boron chemotherapeutics, boron supplements and new drugs. Reliable scientific studies on boron compounds will facilitate the clear presentation of their functions in its biological applications and metabolism. In this study, boron monoester and boron diester structures were synthesized with salicylic acid ligand. To stabilize boron ester structures, Na+, K+, Mg2+, Ca2+ cations were used as counter-ions. Structural properties of the synthesized substances, molecules obtained by crystallization/precipitation from aqueous solutions in solid state, elemental analysis, melting point determination, infrared spectroscopy analysis (FT-IR), thermal analysis (TGA/DTA), mass analysis (GC-MS) and single crystal analysis. Structural properties were tried to be explained by structure analysis (SC-XRD) methods. Additionally, the anticancer potential of boron salicylate esters against the MDA-MB-231 human breast adenocarcinoma cell line was examined. The K-B salicylate diester molecule was found to have the most potential potency with the lowest IC50 value against the MDA-MB-231 cell line. The anticancer potential of boron salicylate esters can be further investigated with other cancer models with the combination of anticancer drugs. It is also thought that the mechanism of action of these molecules may help reveal their further applications.

Roles of defects in perovskite CsPbX3 (X=I, Br, Cl): a first- principles investigation

Inorganic lead halide perovskite CsPbX3 (X=I, Br, Cl) have a promising application in optoelectronic fields due to their excellent photovoltaic properties. The defects, which have a significant impact on the performance of materials, are often introduced during the synthesis process. However, there is still a lack of systematic theoretical investigation of the effects of these defects. In this study, the effects of vacancies and H-atom interstitial point defects on the structural, electronic and optical properties of CsPbX3 are systematically investigated by using first-principles approach based on density-functional theory. The calculated results show that the introduction of different defects have significantly effect on the band gap, effective mass, semiconductor properties, ion migration and optical absorption coefficient of the perovskite materials. It is also found that VCs and VPb defects introduce shallow transition levels that do not negatively impact the optoelectronic properties. However, VX and interstitial H defects generate deep transition levels within the bandgap, which acts as non-radiative recombination centers and reduce the optoelectronic performance of the perovskite material. This study contributes to the understanding of the nature of halide chalcogenides and optimally regulating the performance of optoelectronic devices.

Buckwheat containing-bread: a scientific inquiry into insulin, polyphenols, antioxidants status, and oxidative stress markers in type-II diabetic individuals

Buckwheat grains contain bioactive components with known effects on cardio-metabolic biomarkers. Previous research on oxidative stress markers made exclusively from wheat flour or two-hour postprandial levels of antioxidant status, plasma total polyphenols, and advanced glycation end products (AGEs) were considerably higher than fasting values after consuming buckwheat-incorporated bread (BWB), including clinical, animal, and epidemiological studies, suggesting the potential of buckwheat intake to influence parameters such as polyphenol intake, antioxidant status, insulin levels, and oxidative stress markers. However, the specific impact of buckwheat-containing bread on these biomarkers in individuals with type-II diabetes remains a significant gap in our understanding. Our current work aims to address this gap by exploring the effects of buckwheat-containing bread on insulin levels, polyphenol intake, antioxidant status, and markers of oxidative stress. In a randomized crossover study, 16 individuals with type-II diabetes were assigned to consume control bread made exclusively from wheat flour or bread incorporating 50% buckwheat flour. The research followed a crossover design, with a period of one to two washout interventions. Test breads were given at breakfast following a 12 h fast the previous night. After 2 h of bread intake, blood samples were collected at baseline (fasting). The two-hour postprandial levels of antioxidant status, plasma total polyphenols, and advanced glycation end products (AGEs) were significantly higher than fasting values after consuming buckwheat-incorporated bread (BWB). This suggests that the buckwheat-containing bread improved plasma total polyphenol levels and total antioxidant status. Our research concludes that the intake of bread containing buckwheat positively influences polyphenols in plasma and the status of antioxidants. This suggests that incorporating buckwheat into bread may have favorable effects on biomarkers associated with cardio-metabolic health in individuals with type-II diabetes. By addressing these knowledge gaps, our research aims to inform and engage the scientific community in further exploration of the potential health benefits of buckwheat-containing bread.

RNA transcription assisted universal CRISPR/Cas12a system for programmable analysis of multiple colorectal cancer-associated microRNAs

Accurate analysis of multiple microRNA (miRNA) levels is significantly valuable for early diagnosis of colorectal cancer noninvasively considering the miRNA expression is highly relevant to the occurrence and progression of cancer. However, the low abundance and high sequence homology of miRNAs make their precise determination extremely challenging. Here, we developed a universal and programmable diagnostic strategy allowing for analyzing multiple colorectal cancer-associated miRNAs. The system combined sequentially programmable rolling circle transcription (RCT) and the CRISPR/Cas12a system with high trans-cleavage activity to achieve highly sensitive and specific detection of four target miRNAs. Owing to the remarkable performance of universal RCT-Cas12a strategy, this biosensor could detect miR-21, miR-17, miR-31 and miR-92a with a LOD of 2.1, 1.6, 3.7 and 1.0 pM, respectively. This strategy had a unique advantage in distinguishing human normal colon epithelial cells lines (NCM460) from human colon cancer cells (HT29). In particular, the designed system exhibited superior analytical capability in distinguishing paracancerous and colorectal cancer tissues from patients undergoing colorectal cancer surgery. This arbitrarily programmable, scalable, fast and specific strategy potentially offered an attractive alternative to handle varied challenges encountered with CRISPR-based systems, and held immense promise in scientific research and clinical applications.

Using immersive virtual environments for teaching and socializing children with autism spectrum disorder

The authors' research interests are focused on the development of preschool children with autism spectrum disorder and the potential of virtual reality technologies for teaching and developing children in a gaming environment. The article reviews articles published in peer-reviewed journals and posted in the Scopus database and the Google Scholar search engine for scientific publications over the past 10 years. The objective of the review is to identify international research experience regarding the impact of virtual environments and hardware on children with autism spectrum disorder, their potential effectiveness, recommended conditions and limitations of using virtual reality for social adaptation, development of communication, cognitive and motivational skills. The articles analyzed in the review focus on the following issues: the potential and results of using different types of virtual reality technologies for developing various skills and abilities in children with autism; using virtual reality to work with problems and risks; as well as the features, limitations and safety of using virtual reality headsets by children with autism spectrum disorder. It is recommended to use immersive virtual environments for children with autism under the following conditions: the presence of close and trusted people (caregivers or parents), the presence of real game objects for switching children after exiting the virtual environment and distracting attention from possible negative effects of using gadgets, continuing communication with the child after a virtual reality session in a normal physical space to practice the skill being taught, specific time limits for staying in the virtual environment, etc. It is concluded that the results of using virtual reality technologies remain contradictory, but valid data from scientific research allow us to assert that the use of immersive virtual environments is acceptable, can be effective for teaching children with autism spectrum disorder and, if certain conditions are met, is adequately perceived by them.

THE ROLE OF THE EUROPEAN UNION IN ACHIEVING SUSTAINABLE DEVELOPMENT AT THE GLOBAL LEVEL

In the context of globalization and demographic change, states are currently more vulnerable to non-military, asymmetric, hybrid and cyber threats and threats from non-state actors. Russia's war on Ukraine, Hamas's military attack on Israel indicate the likelihood of armed aggression against the sovereignty and territorial integrity of the state in modern global conditions. In the introductory part of the paper, the author analyzes the notion of national security as the ability of states, taking into account global changes and development, to protect their own identity, survival and interests. Furthermore, the author analyzes the need to achieve Sustainable Development at the global level. In the final part of the paper, the author states that Investing in the common future with its closest neighbors is fundamental for the long-term political stability of the European Union itself. Therefore, the enlargement of the European Union is based on development programs aimed at achieving political stability, secure economic conditions, social cohesion and environmental sustainability. The content will be created based on the analysis of foreign literature and using electronic content. In preparing the content of the paper, the author will apply the general scientific methods: the descriptive method, the normative method, the comparative method and the content analysis method as a special scientific method.